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Ahn Jungmin,Choi Ji Eun,Kang Ju Yong,Choi Ik Joon,Lee Myung-Chul,Lee Byeong-Cheol,Hong Sung Hwa,Moon Il Joon 대한청각학회 2021 Journal of Audiology & Otology Vol.25 No.2
Background and Objectives: Non-linear frequency compression (NLFC) technology compresses and shifts higher frequencies into a lower frequency area that has better residual hearing. Because consonants are uttered in the high-frequency area, NLFC could provide better speech understanding. The aim of this study was to investigate the clinical effectiveness of NLFC technology on the perception of speech and music in patients with high-frequency hearing loss.Subjects and Methods: Twelve participants with high-frequency hearing loss were tested in a counter-balanced order, and had two weeks of daily experience with NLFC set on/off prior to testing. Performance was repeatedly evaluated with consonant tests in quiet and noise environments, speech perception in noise, music perception and acceptableness of sound quality rating tasks. Additionally, two questionnaires (the Abbreviated Profile of Hearing Aid Benefit and the Korean version of the International Outcome Inventory-Hearing Aids) were administered.Results: Consonant and speech perception improved with hearing aids (NLFC on/off conditions), but there was no significant difference between NLFC on and off states. Music perception performances revealed no notable difference among unaided and NLFC on and off states. The benefits and satisfaction ratings between NLFC on and off conditions were also not significantly different, based on questionnaires, however great individual variability preferences were noted.Conclusions: Speech perception as well as music perception both in quiet and noise environments was similar between NLFC on and off states, indicating that real world benefits from NLFC technology may be limited in Korean adult hearing aid users.
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Sung, Hye Youn,Yang, San-Duk,Park, Ae Kyung,Ju, Woong,Ahn, Jung-Hyuck Yonsei University, College of Medicine 2017 Yonsei medical journal Vol.58 No.1
<P><B>Purpose</B></P><P>Ovarian cancer (OC) is the most fatal of gynecological malignancies with a high rate of recurrence. We aimed to evaluate the expression of <I>solute carrier family 6, member 12 (SLC6A12)</I> and methylation of its promoter CpG sites in a xenograft mouse model of metastatic OC, and to investigate the regulatory mechanisms that promote aggressive properties during OC progression.</P><P><B>Materials and Methods</B></P><P>Expression of <I>SLC6A12</I> mRNA was determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR), and DNA methylation status of its promoter CpGs was detected by quantitative methylation-specific PCR. The metastatic potential of <I>SLC6A12</I> was evaluated by <I>in vitro</I> migration/invasion transwell assays. Gene expression and DNA methylation of <I>SLC6A12</I> and clinical outcomes were further investigated from publicly available databases from curatedOvarianData and The Cancer Genome Atlas.</P><P><B>Results</B></P><P><I>SLC6A12</I> expression was 8.1–14.0-fold upregulated and its DNA methylation of promoter CpG sites was 41–62% decreased in tumor metastases. After treatment with DNA methyltransferase inhibitor and/or histone deacetylase inhibitor, the expression of <I>SLC6A12</I> was profoundly enhanced (~8.0-fold), strongly supporting DNA methylation-dependent epigenetic regulation of <I>SLC6A12</I>. Overexpression of <I>SLC6A12</I> led to increased migration and invasion of ovarian carcinoma cells <I>in vitro</I>, approximately 2.0-fold and 3.3-fold, respectively. The meta-analysis showed that high expression of <I>SLC6A12</I> was significantly associated with poor overall survival [hazard ratio (HR)=1.07, <I>p</I> value=0.016] and that low DNA methylation levels of <I>SLC6A12</I> at specific promoter CpG site negatively affected patient survival.</P><P><B>Conclusion</B></P><P>Our findings provide novel evidence for the biological and clinical significance of <I>SLC6A12</I> as a metastasis-promoting gene.</P>
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Ahn, Myung-Ju,Noh, Yun-Hee,Kim, Wo-Chul,Kim, Hyun-Soo,Lee, Young-Yul,Jung, Tae-June,Choi, Il-Young,Kim, In-Soon,Lee, Young-Sung 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.1
고용량 항암 치료와 자가조혈모세포 이식을 받은 고위험군 및 전이성 유방암 환자에서 채취한 말초조혈모세포의 암세포오염을 검출하기 위해 암세포 표지자의 하나인 CEA mRNA를 역전사중합연쇄반응을 이용하여 연구하였다. 말초혈액조혈모세포는 G-CSF 단독 또는 항암제와 G-CSF을 이용하여 가동화 하였다. 음성 대조군인 9예의 정상인의 말초혈액에서는 CEA mRNA가 검출되지 않은 반면, 7예의 고위험군 중 3예(42.8%)과 21예의 전이성 유방암 환자중 6예(28.5%)에서 각각 CEA mRNA가 검출되었다. 항암제 및 G-CSF로 가동한 18예 중 5예(27.8%)에서 CEA mRNA가 검출되었고, G-CSF만으로 가동화한 경우 10예 중 4예(40%)에서 검출되었다. 고위험군에서 CEA mRNA 양성군의 평균 림프절전이수는 32.3으로 음성군의 14보다 많았다. 전이성 유방암 환자에서 무재발생존기간 또는 생존기간에서 두군간에 차이는 없었다. 본 저자들은 많은 수의 고위험군 및 전이성 유방암 환자의 말초혈액조혈모세포에 암세포의 오염이 있음을 확인하였고, CEA mRNA를 표지자로 역전사중합연쇄반응을 이용한 암세포 검출은 향후 자가조혈모세포 이식후의 재발 등을 추적하는데 유용한 지표로 사용될 것으로 사료된다. 본 연구에서 암세포의 오염이 이식후 재발이나 생존에 어떠한 영향을 미치는가에 대하서 적은 환자수와 짧은 추적기간으로 결론 내리기는 어려우나 향후 좀 더 많은 수의 환자를 대상으로 장기적인 추적관찰을 통해 말초조혈모세포에서의 암세포 오염 임상적 의의를 밝힐수 있으리라 기대된다. We analyzed the peripheral blood stem cells obtained from patients with high risk or metastatic breast cancer undergoing high dose chemotherapy with autologous peripheral blood stem cell transplantation to assess the presence of CEA mRNA by RT-PCR(reverse transcription-polymerase chain reaction) as an indicator of malignant cell contamination and to evaluate its clinical significance. The peripheral blood stem cells were obtained from chemotherapy and/or G-CSF mobilization. A total of 7 high risk and 21 metastatic breast cancer patients and nine normal healthy subject were studied. No CEA mRNA was detected in all the nine normal subjects. Three out of 7 (42.8%) high risk and 6 out of 21 (28.5%) metastatic breast cancer were found to be positive for CEA mRNA. CEA mRNA was found in the apheresis products of 27.8%(5/18) of patients mobilized with chemotherapy plus G-CSF and 40%(4/10) of patients with G-CSF alone. In high risk patients, the number of lymph node metastasis in positive group was higher than that of negative group (mean 32.3 vs 14). In metastasis breast cancer, there was no significant difference between CEA mRNA positive and negative group in progression free or overall survival. Our study suggests that a significant number of high risk of metastatic breast cancer patients undergoing autologous transplantation are contaminated with circulating malignant cells in apheresis product. This assay may be useful in monitoring tumor cell contamination and in developing prognostic models for the risk of relapse after transplantation. Although it is unclear what role the contaminating malignant cells have in relapse in our study, further studies with large number of patients and the comparison with other prognostic factors and characteristics of the tumor may determine the significance of tumor contamination in apheresis products.
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Ahn, Myung-Ju,Choi, Jung-Hae,Shim, Sung-Gon,Na, Kyoung-Sun,Kim, Think-You,Bae, Sang-Cheol 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2
Systemic sclerosis (SSc) is a devastating systemic disease with diffuse scleroderma and visceral organ involvement is associated with high morbidity and mortality. Autologous hematopoietic stem cell transplantation (AHSCT) has been developed as a potential treatment for autoimmune disease based on the animal model and clinical evidence. Here, we report a case of 31-year-old patient with rapidly progressive diffuse scleroderma successfully treated with autologous HSCT. Peripheral blood stem cells were mobilized with cyclophosphamide (CTX, 3 g/㎡), followed by granulocyte-colony stimulating factor (G-CSF, 5μg/kg/day). The CD34 positive selected numbers were 4.33×10^(6) cells/kg. Conditioning regimen was high dose CTX (total dose 200 mg/kg) and antithymocyte globulin (ATG, total dose 90 mg/kg). The patient tolerated this regimen well with manageable toxicity. The neutrophil count (>500μ/L) was recovered by day 9. Only transient thrombocytopenia occurred on day 4. Two months after HSCT, the patient's total skin score was decreased by 55% accessed by the modified Rodnan skin thickness score (from 27 to 12). The health-related quality of life, which was measured by Korean Short Form Health Survey-36 (KSF-36) and EuroQol-5 Dimensions (KEQ-5D), was improved. And the functional disability, which was assessed by the Korean Health Assessment Questionnaire-20 (KHAQ-20), also showed an improvement.
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감자유전자 StACRE의 분리 및 풋마름병 저항성 기능 검정
Sang Ryeol Park(박상렬),Eun-Mi Cha(차은미),Tae Hun Kim(김태훈),Seyoun Han(한세연),Duk-Ju Hwang(황덕주),Il-Pyung Ahn(안일평),Kwang-Soo Cho(조광수),Shin-Chul Bae(배신철) 한국생명과학회 2012 생명과학회지 Vol.22 No.2
Ralstonia solanacearum (Rs)에 의해 유발되는 풋마름병은 감자 재배 시 발병하는 주요 병 중의 하나이다. 감자에서 풋마름병 저항성관련 유전자를 찾기 위해 기존에 기능이 알려진 다른 가지과 작물의 기능 유사 유전체를 이용하여 StACRE (HM749652) 유전자를 분리하고 염기서열을 분석하였다. 분리한 StACRE의 발현양상을 분석하기 위해 병 저항성 유도 신호전달 물질인 SA와 풋마름병원균 Rs (KACC10722)를 처리한 감자에서 RNA를 추출하여 RT-PCR을 실시한 결과 이 유전자는 SA 처리에 의해 3시간 후부터, Rs에 의해서는 12시간 후부터 발현이 현저하게 증가하였다. 따라서, 감자에서 이 유전자의 생물학적인 기능을 분석하기 위해 Gateway System을 이용하여 과발현용 vector를 만든 후 과발현 형질전환 감자를 제작하고 풋마름병균인 Rs를 접종하여 병 저항성 기능을 검정 한 결과 대조구인 수미 감자에 비해 병 저항성이 증대하였다. Bacterial wilt (brown rot) caused by Ralstonia solanacearum (Rs) is one of the most devastating bacterial plant diseases in potatoes. To isolate bacterial wilt disease resistance-related genes from the potato, the StACRE (HM749652) gene was isolated and a sequenced search was performed using functional orthologs of Solanaceae from potatoes. StACRE is homologous to the tobacco NtACRE 132 protein and belongs to the ATL family involved in ubiquitination. To analyze the expression pattern of this gene, RT-PCR was performed with potato treated with salicylic acid (SA) and Rs (KACC 10722). StACRE was strongly induced 3 hours after treatment with SA and 12 hours after infection with Rs. To investigate its biological functions in the potato, we constructed a vector for overexpression in the potato by the Gateway system, and then generated transgenic potato plants. The gene expression of transgenic potato was analyzed by northern blot analysis. In the results of disease resistance assay in relation to bacterial wilt, StACRE overexpressed transgenic potato plants were shown to have more resistance than wild-type potato.
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AHN, EUN HEE,KIM, DAE WON,SHIN, MIN JEA,RYU, EUN JI,YONG, JI IN,CHUNG, SEOK YOUNG,CHA, HYUN JU,KIM, SANG JIN,CHOI, YEON JOO,KIM, DUK-SOO,CHO, SUNG-WOO,LEE, KEUNWOOK,CHO, YOON SHIN,KWON, HYEOK YIL,PARK UNKNOWN 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.38 No.1
<P>Antioxidant 1 (ATOX1) functions as an antioxidant against hydrogen peroxide and superoxide, and therefore may play a significant role in many human diseases, including diabetes mellitus (DM). In the present study, we examined the protective effects of Tat-ATOX1 protein on streptozotocin (STZ)-exposed pancreatic insulinoma cells (RINm5F) and in a mouse model of STZ-induced diabetes using western blot analysis, immunofluorescence staining and MTT assay, as well as histological and biochemical analysis. Purified Tat-ATOX1 protein was efficiently transduced into RINm5F cells in a dose-and time-dependent manner. Additionally, Tat-ATOX1 protein markedly inhibited reactive oxygen species (ROS) production, DNA damage and the activation of Akt and mitogen activated protein kinases (MAPKs) in STZ-exposed RINm5F cells. In addition, Tat-ATOX1 protein transduced into mice pancreatic tissues and significantly decreased blood glucose and hemoglobin A1c (HbA1c) levels as well as the body weight changes in a model of STZ-induced diabetes. These results indicate that transduced Tat-ATOX1 protein protects pancreatic beta-cells by inhibiting STZ-induced cellular toxicity in vitro and in vivo. Based on these findings, we suggest that Tat-ATOX1 protein has potential applications as a therapeutic agent for oxidative stress-induced diseases including DM.</P>
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