한방 이화주의 미백 및 피부 주름 개선 효과

이상진(Sang-Jin Lee),권이영(Yi-Young Kwon),조성원(Sung-Won Cho),권희숙(Hee-Suk Kwon),신우창(Woo-Chang Shin) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.4

본 연구에서는 한방이화주의 피부 생리기능 활성을 알아보기 위해 70% EtOH 추출물의 피부 미백, 주름 개선 및 항염증 효과를 조사하였다. HEE은 tyrosinase 활성 억제 및 tyrosine을 기질로 melanin이 형성되는 pathway에 관여하는 주요한 인자인 TRP-1과 TRP-2를 저해하는 작용 기전을 통해 피부 색소침착의 주요 원인 물질인 melanin 생합성을 농도 의존적으로 저해하는 것을 확인하였다. HEE은 피부진피 내 피부 탄력을 유지하는 elastin을 분해하는 효소인 elastase의 활성을 저해하였고, 피부의 keratinocyte가 생성분비하는 MMP-2와 MMP-9의 단백질 발현과 proteolytic 활성을 억제하여 노화에 따른 피부 주름 생성 억제할 수 있는 가능성을 확인하였다. 또한 세포 독성 없이 LPS에 의해 유도된 염증 반응을 50% 저해하는 HEE의 농도(IC50)는 24.9 μg/mL이며, 50 μg/mL 농도로 처리하였을 때 염증 반응 저해 효과가 70%로 높은 효과를 가지는 것을 확인하였다. 이상의 결과를 종합하면 HEE의 피부 미백, 주름 개선 및 항염증에 우수한 효과를 나타내고 있으므로 기능성 화장품의 주요한 소재로 이용 가치가 높을 것으로 사료된다. Extrinsic skin aging is characterized by the loss of skin tone and resilience, irregular pigmentation, and deep wrinkles. The aim of this study was to investigate the effects of Ehwa Makgeolli containing oriental herbs (Glycyrrhiza uralensis Fisch., Lycium chinense MILL., Morus alba L., and Saururus chinensis Baill) on skin whitening and wrinkling in human skin cells. We prepared Makgeolli extracts (HEE) with 70% ethanol. HEE significantly inhibited in vitro mushroom tyrosinase activity and reduced the cellular and secreted melanin content of mouse melanoma melanocytes (B16F1 cells). HEE down-regulated the protein expression of tyrosinase related protein (TRP)-1/-2, a key player in melanogenesis. Treatment with HEE in human keratinoctyes (HaCaT cells) inhibited the proteolytic activities of matrix metalloproteinase (MMP)-2/-9 in a dose-dependent manner and dramatically reduced the expression of MMP-2/-9. In addition, HEE attenuated lipopolysaccharide (LPS)- induced nitric oxide production in murine macrophages (RAW264.7 cells). These results indicate that HEE may be a great cosmeceutical ingredient for its whitening, anti-wrinkle, and anti-inflammatory effects.

A Study of the Yang-gyeong-gyu-il-ui (兩景揆日儀) in the Joseon Dynasty

Lee, Yong Sam,Kim, Sang Hyuk,Mihn, Byeong-Hee The Korean Space Science Society 2015 Journal of Astronomy and Space Sciences Vol.32 No.1

The Yang-gyeong-gyu-il-ui (兩景揆日儀) is a kind of elevation sundial using three wooden plates. Sang-hyeok Lee (李尙爀, 1810~?) and Byeong-cheol Nam (南秉哲, 1817~1863) gave descriptions of this sundial and explained how to use it in their Gyu-il-go (揆日考) and Ui-gi-jip-seol (儀器輯說), respectively. According to Gyu-il-go (揆日考) there are two horizontal plates and two vertical plates that have lines of season and time. Subseasonal (節候) lines are engraved between seasonal (節氣) lines, subdividing the interval into three equal lines of Cho-hu (初候, early subseason), Jung-hu (中候, mid subseason) and Mal-hu (末候, late subseason); there are 13 seasonal lines for a year, thus resulting in 37 subseasonal lines; also, there are 12 double-hour (時辰) lines for a day engraved on these plates. The only remaining artifact of Yang-gyeong-gyu-il-ui was made in 1849 (the $15^{th}$ year of Heon-jong) and is kept at the Korea University Museum. We have compared and analyzed Yang-gyeong-gyu-il-ui and similar western sundials. Also, we have reviewed the scientific aspect of this artifact and built a replica. Yang-gyeong-gyu-il-ui is a new model enhanced from the miniaturization development in the early Joseon Dynasty and can be applied to the southern part of the tropic line through a structure change.

Plenary Session 2 : PS-2-1 ; Outcomes of hepatitis B virus recurrence after liver transplantation: a multicenter analysis in Korea

( Hee Yeon Kim ),( Jong Young Choi ),( Dong Goo Kim ),( Myoung Soo Kim ),( Soon Il Kim ),( Shin Hwang ),( Sung Gyu Lee ),( Kwang Woong Lee ),( Kyung Suk Suh ),( Young Seok Han ),( Dong Lak Choi ),( Se 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: The outcome of hepatitis B virus (HBV) infection after liver transplantation (LT) was improved by hepatitis B immunoglobulin (HBIG) and nucles(t)ide analogue (NUA). However, HBV recurrence after LT is critical because the recurrence is occasionally accompanied by a progressive destruction of graft and poor survival. The aims of this study were to investigate the significance HBV recurrence and identity factors associated with HBV recurrence. Methods: From October 1999 to February 2011, a total of 2684 consecutive LT recipients who underwent HBV-associated LT were retrospectively enrolled from 7 transplantation centers in Korea. Results: Prophylaxis regimens were HBIG monotherapy (67.7%) or a combination of HBIG with NUA (22.3%). The recurrence rate of HBV was 6.1% (164 recipients) during mean follow-up duration of 10.9 years. The median time from transplantation to recurrence was 2.1 years (0.1-7.9 years). Of the 1,071 patients with hepatocellular carcinoma (HCC) prior to LT, 155 patients (14.5%) had HCC recurrence after transplantation, and 48 patients (31.0%) had HBV recurrence. Of the 48 patients with recurrence of both HBV and HCC, 25 patients (52.1%) experienced HBV recurrence after HCC recurrence. In the multivariate analysis, pretransplant HCC, pretransplant HBV DNA above 5.5 log copies/mL was independent clinical factors influencing HBV recurrence after LT. The mortality rate among the recipients with HBV recurrence was 34.1% (56 recipients). Mean overall survival was 6.4 years in the HBV-recurrence group and 9.9 years in the HBV-nonrecurrence group (p<0.001). HBV recurrence was not an independent prognostic factor for overall survival. HCC recurrence was the most important factor for overall survival. Conclusions: The overall outcome of LT in HBV-related liver disease was excellent with the current prophylaxis regimen Choon Hyuck David Kwon,8 Suk-Koo Lee8 Pretransplant HBV DNA and HCC were important factors for HBV recurrence. HBV recurrence after LT did not significantly influence on the overall survival without combining of HCC recurrence.

Studies on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+ , P and ALP Activity in Osteoporosis-Induced Rats

Sang-keun Kim,Myung-hun Lee,Man-hee Rhee 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+, P andALP Activity in Osteoporosis-Induced RatsSang-keun Kim, Myung-hun Lee1 and Man-hee Rhee2College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yusong-Gu, Daejeon 305-764, Korea1National Veterinary Research and Quarantine Service, Anyang 430-824, Korea2College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived April 3, 2003 / Accepted July 9, 2003J. Vet. Sci. (2003), 4(2), 151-154JOURNAL OFVeterinaryScience*Corresponding author: Sang-keun Kim Dept. of Vet. Med., Chungnam National University, Daejeon 305764, Korea Tel: +82-42-821-6754, Fax: +82-42-821-6754 E.-mail: kskkim@cnu.ac.kr

Antigastritis effects of <i>Armillariella tabescens</i> (Scop.) Sing. and the identification of its anti-inflammatory metabolites

Lee, Seulah,Lee, Dahae,Park, Jun Yeon,Seok, Soonja,Jang, Tae Su,Park, Hyun Bong,Shim, Sang Hee,Kang, Ki Sung,Kim, Ki Hyun Pharmaceutical Society of Great Britain 2018 Journal of pharmacy and pharmacology Vol.70 No.3

The Influence of Anxiety and Dyadic Adjustment on Maternal–Fetal Attachment in High-Risk Pregnant Women

Sung–Hee Lee,Eun-Young Lee 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.12

Purpose: This study attempted using a descriptive survey to elucidate the influence of anxiety and dyadic adjustment on maternal–fetal attachment in high-risk pregnant women. Methods: The data used in this study were collected from March 3rd, 2015, to March 30th, 2015, and the participants were 118 pregnant women including those undergoing prenatal tests and those admitted to a delivery room in the obstetrics outpatient center of 3 university hospitals located in B, D, and Y after diagnosis with high-risk pregnancy during 20–38 weeks of gestation. Collected data were analyzed using frequency, percentage, mean, standard deviation, t-test, ANOVA, Pearson’s correlation, and stepwise regression analysis using the IBM SPSS 22.0 program. Results: The level of maternal–fetal attachment according to participants’ general and obstetric characteristics showed significant differences in history of childbirth, prenatal tests, and planned pregnancy. The factors that influenced maternal–fetal attachment were history of childbirth, prenatal tests, anxiety, and dyadic adjustment. Lower anxiety and high dyadic adjustment of high-risk pregnant women led to a high maternal–fetal attachment. Among them, anxiety was the factor with the greatest impact, explaining 20.5%. Conclusion: This study presents the need for development and application of prenatal nursing intervention to enhance maternal–fetal attachment by lowering anxiety through prenatal care in high-risk pregnant women and improving dyadic adjustment.

Performance of electrochemical double layer capacitors using highly porous activated carbons prepared from beer lees

Sang Guk Lee,Hee Moon,Kwang Hyun Park,심왕근,M.S. balathanigaimani 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3

Beer lees precursor is chosen to prepare activated carbons having different physical and chemical properties. The beer lees-based activated carbons (BL-ACs) are characterized by N2 adsorption/desorption isotherms, adsorption energy distributions (AEDs), and X-ray photoelectric spectroscopy (XPS). Furthermore the electrochemical properties of the BL-ACs are assessed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and constant current charge/discharge method. The maximum specific capacitance (about 188 F/g at discharge current of l mA/cm2) is obtained in 0.1 M H2SO4 electrolyte solution, which is a relatively low electrolyte concentration. The overall results suggest that the BL-ACs are good candidates for EDLC electrode materials.

자동차 탑승자 사고에서 외상계수를 이용한 구급대원의 중증도 분류 평가

김상철 ( Sang Chul Kim ),김병우 ( Byung Woo Kim ),탁양주 ( Yang Ju Tak ),이상희 ( Sang Hee Lee1 ) 대한외상학회 2013 大韓外傷學會誌 Vol.26 No.3

Purpose: The assessment of trauma patients in the prehospital setting is difficult, but appropriate field triage is critical to the prognosis of trauma patients. We sought to evaluate the triage given by the emergency medical technicians (EMTs) using the trauma score to patients injured in motor vehicle collisions (MVCs). Methods: From June 2012 to July 2012, questionnaires were distributed to EMTs, who had transported injured patients to the study hospital. Scene records, photos of the damaged vehicle, and ambulance run sheets were used to provide physiologic, physical, and mechanistic information about the MVC. To evaluate the appropriateness of the injury assessment by EMTs, we compared their impressions with the hospital`s final diagnosis within a 3 level triage system comprising both the maximum abbreviated injury scale (MAIS) and the injury severity score (ISS). Kappa (k) was calculated to evaluate the agreement between the triage by EMTs and the triage based on hospital`s final diagnosis. Results: A total of 91 patients were analyzed by 31 EMTs. The percentage of males was 57.1%, the mean age was 44.5, and the mean MAIS and ISS were 2.7 and 16.6 respectively. While EMTs correctly diagnosed patient injuries to the extremities in 35.7%, and to the neck in 32.1%, pelvic injuries were missed in 80.0%. The agreement between the triage by the EMTs and the triage based on the hospital`s final diagnosis was 62.6%(k=0.366) by the MAIS and 50.5%(k=0.234) by the ISS. The kappa value was higher in EMT-I than in EMT-II. Conclusion: In MVC, the assessment of injured patients by EMT-I was more appropriate, and the 3-level triage method based on the MAIS could contribute to a more accurate triage. Prospective studies to search for appropriate methods of field triage are required for programming practical education for EMTs.

BEZ235 (PIK3/mTOR inhibitor) Overcomes Pazopanib Resistance in Patient-Derived Refractory Soft Tissue Sarcoma Cells

Kim, Hee Kyung,Kim, Sun Young,Lee, Su Jin,Kang, Mihyeon,Kim, Seung Tae,Jang, Jiryeon,Rath, Oliver,Schueler, Julia,Lee, Dong Woo,Park, Woong Yang,Kim, Sung Joo,Park, Se Hoon,Lee, Jeeyun Neoplasia Press 2016 Translational oncology Vol.9 No.3

<P><I>BACKGROUND:</I> Although pazopanib treatment has become the standard chemotherapy in salvage setting for metastatic sarcoma patients, most patients progress after pazopanib treatment in 4 to 6 months. After failure to pazopanib, patients have limited options for treatment. Therefore, subsequent therapy in patients who failed to pazopanib is urgently needed and the use of patient derived cells or patient derived tumors for accompanying testing with various pharmacological inhibitors could offer additional treatment options for these patients. <I>METHODS:</I> Patient derived tumor cells were collected from ascites at the time of progression to pazopanib and a 13-drug panel was tested for drug sensitivity. We confirmed the results using <I>in vitro</I> cell viability assay and immunoblot assay. We also performed the genomic profiling of PDX model. <I>RESULTS:</I> The growth of patient derived tumor cells was significantly reduced by exposure to 1.0 μM AZD2014 compared with control (control versus AZD2014, mean growth = 100.0% vs 16.04%, difference = 83.96%, 95% CI = 70.01% to 97.92%, <I>P</I> = .0435). Similarly, 1.0 μM BEZ235 profoundly inhibited tumor cell growth <I>in vitro</I> when compared to control (control versus BEZ235, mean growth = 100.0% vs 7.308%, difference = 92.69%, 95% CI = 78.87% to 106.5%, <I>P</I> < .0001). Despite the presence of CDK4 amplification in the patient-derived tumor cells, LEE011 did not considerably inhibit cell proliferation when compared with control (control vs LEE011, mean growth = 100.0% vs 80.23%, difference = 19.77%, 95% CI = 1.828% to 37.72%, <I>P</I> = .0377). The immunoblot analysis showed that BEZ235 treatment decreased pAKT, pmTOR and pERK whereas AZD2014 decreased only pmTOR. <I>CONCLUSION:</I> Taken together, upregulation of mTOR/AKT pathway in sarcoma patient derived cells was considerably inhibited by the treatment of AZD2014 and BEZ235 with downregulation of AKT pathway (greater extent for BEZ235). These molecules may be considered as treatment option in STS patient who have failed to pazopanib in the context of clinical trials.</P>

수사적 상황과 역할에 따른 대통령 수사학 장르 분석

박성희(Sung Hee Park),이자원(Jawon Lee),석지윤(Ji Yoon Seok) 한국수사학회 2013 수사학 Vol.0 No.19

Rhetorical genre represents a class of messages having important structural and content similarities that creates special expectations among listeners. Presidential rhetoric delivers textual features to the similar effect of creating trust and bonding people. Based on the arguments of Bitzer and Hart, this study attempts to compare and contrast two presidential addresses that were delivered in two different time and spacial settings. For this purpose, former President Park Jung Hee’s 1963 National Press Club address in Washington and President Park Geun Hye’s 2013 address in U.S. Congress in Washington were chosen. As analytic tools, Hart’s credibility strategy index and I-reference style were used, along with Bitzer’s elements in rhetorical situation. The results indicated that two Presidents showed similar patterns in their use of credibility strategy and use of I-reference positioning them in a certain role in their speeches. While two speeches, each of which 48 years apart, revealed different rhetorical situations in terms of their exigences, audiences, and contraints, respective presidents retained similar generic features in their addresses. From the results, it could be discussed that the presidential rhetoric as a generic form has a unique construct of communicating messages that pronounce their unique social role and solidate trust system in society.