Drug Target Identification and Elucidation of Natural Inhibitors for Bordetella petrii: An In Silico Study

Rath, Surya Narayan,Ray, Manisha,Pattnaik, Animesh,Pradhan, Sukanta Kumar Korea Genome Organization 2016 Genomics & informatics Vol.14 No.4

Environmental microbes like Bordetella petrii has been established as a causative agent for various infectious diseases in human. Again, development of drug resistance in B. petrii challenged to combat against the infection. Identification of potential drug target and proposing a novel lead compound against the pathogen has a great aid and value. In this study, bioinformatics tools and technology have been applied to suggest a potential drug target by screening the proteome information of B. petrii DSM 12804 (accession No. PRJNA28135) from genome database of National Centre for Biotechnology information. In this regards, the inhibitory effect of nine natural compounds like ajoene (Allium sativum), allicin (A. sativum), cinnamaldehyde (Cinnamomum cassia), curcumin (Curcuma longa), gallotannin (active component of green tea and red wine), isoorientin (Anthopterus wardii), isovitexin (A. wardii), neral (Melissa officinalis), and vitexin (A. wardii) have been acknowledged with anti-bacterial properties and hence tested against identified drug target of B. petrii by implicating computational approach. The in silico studies revealed the hypothesis that lpxD could be a potential drug target and with recommendation of a strong inhibitory effect of selected natural compounds against infection caused due to B. petrii, would be further validated through in vitro experiments.

A Stable Organic Radical Delocalized on a Highly Twisted π System Formed Upon Palladium Metalation of a Möbius Aromatic Hexaphyrin

Rath, Harapriya,Tokuji, Sumito,Aratani, Naoki,Furukawa, Ko,Lim, Jong Min,Kim, Dongho,Shinokubo, Hiroshi,Osuka, Atsuhiro WILEY-VCH Verlag 2010 Angewandte Chemie Vol.122 No.8

<B>Graphic Abstract</B> <P>Ein neuer Dreh bei Porphyrinen: Die Metallierung von Möbius-aromatischem Hexakis(pentafluorphenyl)[28]hexaphyrin ergibt ein stabiles organisches Radikal, das über ein zweifach verdrehtes π-System delokalisiert ist. Der Zweiphotonenabsorptionsquerschnitt ist deutlich höher als bei nichtaromatischen expandierten Porphyrinen (Pd rosa, Cl grün, N blau; ohne Phenylringe). <img src='wiley_img/00448249-2010-122-8-ANGE200906017-content.gif' alt='wiley_img/00448249-2010-122-8-ANGE200906017-content'> </P>

Rate of Oxygen Uptake in Antheraea mylitta in Various Stages of Development and during Diapause

Rath S. S.,Negi B. B. S.,Singh B. M. K.,Thangavelu K. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.10 No.1

Respiration in Antheraea mylitta was studied using constant pressure respirometer. Oxygen uptake in different stages of life showed that moth has highest rate of oxygen uptake as compared to larva and pupa. At each stage significant sexual differentiation was observed. Depression in the rate of oxygen uptake in diapausing pupa was found to be higher in male ($65.75\%$) than female ($60.65\%$) as compared to non-diapausing counterparts. During diapause lowest oxygen uptake was recorded in the month of February, and were in the order of February < January < December < March < November < April < May < June in male, and, February < January < December < March < April < November < May < June in female. Significant sexual differentiation in oxygen uptake was recorded throughout the period of diapause (November to June) where female pupae registered lower rate of oxygen uptake than that of male. Oxygen uptake in female pupae declined upto $28.89\%$ in non-diapausing and $18.29\%$ in diapausing broods over male. Highest respiratory quotient value was recorded in the moth of November (0.68 in male and 0.70 in female) and, the lowest in the month of March in male (0.54) and May in female (0.55). Percentage loss in pupa weight always remained at a significantly higher level in male (except in February and March). The lowest oxygen uptake rate and weight loss in the month of February shows that the pupae were at peak of their dormancy during this month.

In Silico Study of miRNA Based Gene Regulation, Involved in Solid Cancer, by the Assistance of Argonaute Protein

Rath, Surya Narayan,Das, Debasrita,Konkimalla, V Badireenath,Pradhan, Sukanta Kumar Korea Genome Organization 2016 Genomics & informatics Vol.14 No.3

Solid tumor is generally observed in tissues of epithelial or endothelial cells of lung, breast, prostate, pancreases, colorectal, stomach, and bladder, where several genes transcription is regulated by the microRNAs (miRNAs). Argonaute (AGO) protein is a family of protein which assists in miRNAs to bind with mRNAs of the target genes. Hence, study of the binding mechanism between AGO protein and miRNAs, and also with miRNAs-mRNAs duplex is crucial for understanding the RNA silencing mechanism. In the current work, 64 genes and 23 miRNAs have been selected from literatures, whose deregulation is well established in seven types of solid cancer like lung, breast, prostate, pancreases, colorectal, stomach, and bladder cancer. In silico study reveals, miRNAs namely, miR-106a, miR-21, and miR-29b-2 have a strong binding affinity towards PTEN, TGFBR2, and VEGFA genes, respectively, suggested as important factors in RNA silencing mechanism. Furthermore, interaction between AGO protein (PDB ID-3F73, chain A) with selected miRNAs and with miRNAs-mRNAs duplex were studied computationally to understand their binding at molecular level. The residual interaction and hydrogen bonding are inspected in Discovery Studio 3.5 suites. The current investigation throws light on understanding miRNAs based gene silencing mechanism in solid cancer.

Impact of Quantitative Feeding on Nutritional Parameters of Fifth Instar Larvae of Antheraea mylitta

Rath, Sudhansu Sekhar Korean Society of Sericultural Science 2010 International Journal of Industrial Entomology Vol.20 No.2

Feeding is an important aspect of insect nutrition which in turn supports growth, development, reproduction and survival. The impact of quantitative feeding on nutritional parameters of fifth instar Antheraea mylitta larvae was studied by providing fresh leaves of Terminalia tomentosa for 1 to 4 times a day. All the parameters improved upon providing fresh diets more times over the single diet (1FD). The absolute body weight increased with increase in number of feeds/day significantly (P<0.001). While the maximum weight recorded was 29.61 g in 1FD, the respective weights for two feeds/day (2FD), three feeds/day (3FD) and four feeds/day (4FD) were 39.41 g, 40.63 g and 42.66 g respectively. Feeding period and instar duration were declined (P<0.001) and survival increased significantly (P<0.001) upon increase in the number of diets. Nutritional indices like ingestion, digestion, relative consumption rate (RCR), relative growth rate (RGR) and gain in body weight increased significantly with the increase in number of feeds/day, but approximate digestibility (AD) and efficiency of conversion of ingested food (ECI) declined, while efficiency of conversion of digested food (ECD) did not change.

Output Feedback Active Suspension Control With Higher Order Terminal Sliding Mode

Rath, Jagat Jyoti,Defoort, Michael,Karimi, Hamid Reza,Veluvolu, Kalyana Chakravarthy Institute of Electrical and Electronics Engineers 2017 IEEE transactions on industrial electronics Vol. No.

<P>The control of an automotive suspension system using hydraulic actuators is a highly complex nonlinear control task dealing with system nonlinearities, external disturbances, and uncertainties. In this work, an output feedback active suspension control scheme is proposed to achieve a ride comfort while maintaining the road holding for the vehicle. To design the controller, the states of the nonlinear system are first estimated using a highgain observer where the suspension stroke is the only measurable output. The controller is then designed using a recursive derivative nonsingular higher order terminal sliding mode approach that avoids singularity. The practical stability for the closed-loop observer-controller pair is established. Simulation results for the quarter-wheel vehicle over various road conditions demonstrate the effectiveness of the proposed control in improving the suspension performance in both the time and frequency domains.</P>

The effect of root canal irrigants on dentin: a focused review

Rath, Priti Pragati,Yiu, Cynthia Kar Yung,Matinlinna, Jukka Pekka,Kishen, Anil,Neelakantan, Prasanna The Korean Academy of Conservative Dentistry 2020 Restorative Dentistry & Endodontics Vol.45 No.3

Despite the vast literature on the effects of root canal irrigants on the dentin characteristics, the precise effects of clinically relevant irrigation sequences remain unclear. In this review, we systematically dissect the role of different sequential irrigation approaches that are used in clinical endodontics. Using a systematic search strategy, we attempt to answer the question: 'Which irrigating sequence has the most deleterious effects on dentin structure and properties?' The effect of irrigants on the dentin composition and mechanical properties have been reviewed. A wide variety of concentrations, duration and techniques have been employed to characterize the effects of chemicals on dentin properties, thus making it impossible to draw guidelines or recommendations of irrigant sequences to be followed clinically. It was apparent that all the studied irrigation sequences potentially result in some deleterious effects on dentin such as decrease in the flexural strength, microhardness, modulus of elasticity and inorganic content and organic-inorganic ratio of the dentin. However, the literature still lacks comprehensive investigations to compare the deleterious effect of different irrigation sequences, using a wide variety of qualitative and quantitative methods. Such investigations are essential to make clinical recommendations and strategize efforts to minimize chemically-induced damage to dentin characteristics.

Properties and electrochemical performance of Sr_0.8La_0.2TiO_3-δ-Ce_0.8Gd_0.2O_2-δ composite anodes for intermediate temperature solid oxide fuel cells

Rath, M.K.,Lee, K.T. ELSEVIER SCIENCE 2016 JOURNAL OF ALLOYS AND COMPOUNDS Vol.657 No.-

A series of Sr<SUB>0.8</SUB>La<SUB>0.2</SUB>TiO<SUB>3-δ</SUB> (SLT)-Ce<SUB>0.8</SUB>Gd<SUB>0.2</SUB>O<SUB>2-δ</SUB> (GDC) composite anodes with various volume ratios of SLT and GDC has been synthesized using a sucrose modified combustion technique. The percolation thresholds of Sr<SUB>0.8</SUB>La<SUB>0.2</SUB>TiO<SUB>3-δ</SUB> and Ce<SUB>0.8</SUB>Gd<SUB>0.2</SUB>O<SUB>2-δ</SUB> in the composite calculated based on the Kusy's percolation theory were 75.6 vol.% and 36.1 vol.%, respectively. While the rate of carbon deposition increased with an increase in the Ce<SUB>0.8</SUB>Gd<SUB>0.2</SUB>O<SUB>2-δ</SUB> content, the total electrical conductivity decreased in humidified H<SUB>2</SUB> as well as in CH<SUB>4</SUB>. Based on the electrochemical performance measured using AC-impedance spectroscopy, the optimal anode composition was 65 vol.% Sr<SUB>0.8</SUB>La<SUB>0.2</SUB>TiO<SUB>3-δ</SUB>-35 vol.% Ce<SUB>0.8</SUB>Gd<SUB>0.2</SUB>O<SUB>2-δ</SUB>. This composite has polarization resistances of 2.80 @? cm<SUP>2</SUP> and 4.60 @? cm<SUP>2</SUP> in H<SUB>2</SUB> and CH<SUB>4</SUB>, respectively, measured at 800 <SUP>o</SUP>C. Diffusion or concentration polarization was the rate determining steps for the fuel oxidation process in the Sr<SUB>0.8</SUB>La<SUB>0.2</SUB>TiO<SUB>3-δ</SUB>-Ce<SUB>0.8</SUB>Gd<SUB>0.2</SUB>O<SUB>2-δ</SUB> composite anode.

Adaptive Super-Twisting Observer for Estimation of Random Road Excitation Profile in Automotive Suspension Systems

Rath, J. J.,Veluvolu, K. C.,Defoort, M. Hindawi Publishing Corporation 2014 The Scientific World Journal Vol.2014 No.-

<P>The estimation of road excitation profile is important for evaluation of vehicle stability and vehicle suspension performance for autonomous vehicle control systems. In this work, the nonlinear dynamics of the active automotive system that is excited by the unknown road excitation profile are considered for modeling. To address the issue of estimation of road profile, we develop an adaptive supertwisting observer for state and unknown road profile estimation. Under Lipschitz conditions for the nonlinear functions, the convergence of the estimation error is proven. Simulation results with Ford Fiesta MK2 demonstrate the effectiveness of the proposed observer for state and unknown input estimation for nonlinear active suspension system.</P>

Nutritional Efficiency in Antheraea mylitta D. during Food Deprivation

Rath, S.S.,Sinha, B.R.R.P.,Thangavelu, K. Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.9 No.1

Nutrition plays an important role in maintaining the larval health, cocoon quality and reproductive potential in Antheraea mylitta D. Nutritional efficiency greatly influenced if food is not adequate and of quality. A. mylitta silkworms were subjected to food deprivation for the period of 0 hr to 12 hrs /day to assess its effect on various nutritional parameters and indices, and its manifestation at different levels. Food ingesta, digesta, gain in body weight declined significantly at each level of deprivation, so also food utilization efficiency like consumption index (CI), growth rate (GR), approximate digestibility (AD), and efficiency of conversion of ingested food (ECI). This stress leads to decline in mean daily food ingesta by 16.73% to 39.76% and digesta by 28.98% to 54.01 % following a significant reduction in average daily body weight gain (27.68% to 55.09%). Food deprivation a1so caused significant loss in the silk gland weight, cocoon and shell weight (14.37% to 53.69%), lowered the fecundity (35.86 % to 83.59%) and in number of eggs laid per gram body weight, but simultaneously the number of non-chorionated eggs increased significantly.