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ShuangWang,Lei Sun,DanWei,Baoku Zhou,Junzheng Zhang,XuejiaGu,Lei Zhang,Ying Liu,Yidan Li,Wei Guo,Shuang Jiang,Yaqing Pan,Yufeng Wang 한국미생물학회 2014 The journal of microbiology Vol.52 No.7
An alkaliphilic, moderately halophilic, bacterium, designated strain X10-1T, was isolated from saline-alkaline soil in Daqing, Heilongjiang Province, China. Strain X10-1T was determined to be a Gram-positive aerobe with rod-shaped cells. The isolate was catalase-positive, oxidase-negative, non-motile, and capable of growth at salinities of 0–16% (w/v) NaCl (optimum, 3%). The pH range for growth was 7.5–11.0 (optimum, pH 10.0). The genomic DNA G+C content was 47.7 mol%. Its major isoprenoid quinone was MK-7 and its cellular fatty acid profile mainly consisted of anteiso-C15:0, anteiso-C17:0, iso-C15:0, C16:0, and iso-C16:0. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that X10-1T is a member of the genus Bacillus, being most closely related to B. saliphilus DSM15402T (97.8% similarity) and B. agaradhaerens DSM 8721T (96.2%). DNA-DNA relatedness to the type strains of these species was less than 40%. On the basis of the phylogenetic, physiological, and biochemical data, strain X10-1T represents a novel species of the genus Bacillus, for which the name Bacillus daqingensis sp. nov. is proposed. The type strain is X10-1T (=NBRC 109404T =CGMCC 1.12295T).
폐플라스틱 열분해유 분리 및 촉매 업그레이딩을 통한 열분해유 분리공정 개발
( Shuang Wang ),김하나 ( Hana Kim ),이도연 ( Doyeon Lee ),황병욱 ( Byung Wook Hwang ),원유섭 ( Yooseob Won ),김대욱 ( Dae Wook Kim ),남형석 ( Hyungseok Nam ),류호정 ( Ho-jung Ryu ) 한국폐기물자원순환학회(구 한국폐기물학회) 2020 한국폐기물자원순환학회 추계학술발표논문집 Vol.2020 No.-
본 연구에서는 폐플라스틱을 열분해하여 얻은 폐플라스틱 열분해유에 대해 2 kg/hr 규모의 열분해 오일 분리 시스템에서 열 및 촉매 크래킹 실험을 수행하였다. 연료 분리 과정에서 바이오 오일의 특성을 개선하기 위해 Zeolite 4A와 MDC-7 (Cu-based catalyst) 의 두 가지 촉매가 사용되었다. 결과는 중유 (HO, heavy oil) 분획(> C23)이 열 및 촉매 크래킹에 의해 23.6 wt.%에서 19.8 wt.%로 감소되었음을 보여준다. 그리고 바이오 오일의 산소 함량은 3.36 wt.%이나 열 및 촉매 크래킹을 통해 1.76 wt.% (열 크래킹), 0.95 wt.% (Zeolite 4A 촉매크래킹)와 0.75 wt.% (MDC-7 촉매 크래킹)로 감소되었다. 또한 MDC-7 (Cu-based catalyst) 촉매는 경유 (LO: light oil, 19.72 wt.%) 분획 (C6-C10)을 생성하는 데 도움이 되고, Zeolite 4A 촉매는 중간 오일 (MO: mediumoil, 62.42 wt.%) 분획 (C11-C22)을 생성하는 데 도움이 되었다. MDC-7 촉매는 dehydration, decarboxylation와 oligomerization 반응을 주로 일으키지만 Zeolite 4A 촉매는 주로 decarbonylation와 aromatization 반응이 일어나기 때문으로 사료된다. 마지막으로 촉매 크래킹으로 업그레이드 된 폐플라스틱 열분해유의 물성 (HHV, 점도, Cloud & Pour points, PH, 등)은 폐플라스틱 열분해유보다 우수하며 석유 연료에 매우 가깝다. 따라서 열 및 촉매 크래킹 결과를 바탕으로 업그레이드 된 폐플라스틱 열분해유를 얻기 위한 효과적인 촉매 및 공정을 설계하는 데 도움이 될 것이다.
Shuang Wang,Wenju Zhu,Jian Shi,Hua Ji,Surong Huang 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.6
A predictive functional control (PFC) scheme for permanent magnet synchronous motor (PMSM) servo systems is proposed in this paper. The PFC-based method is first introduced in the control design of speed loop. Since the accuracy of the PFC model is influenced by external disturbances and speed detection quantization errors of the low distinguishability optical encoder in servo systems, it is noted that the standard PFC method does not achieve satisfactory results in the presence of strong disturbances. This paper adopted the Kalman filter to observe the load torque, the rotor position and the rotor angular velocity under the condition of a limited precision encoder. The observations are then fed back into PFC model to rebuild it when considering the influence of perturbation. Therefore, an improved PFC method, called the PFC+Kalman filter method, is presented, and a high performance PMSM servo system was achieved. The validity of the proposed controller was tested via experiments. Excellent results were obtained with respect to the speed trajectory tracking, stability, and disturbance rejection.
Shuang Wang,Jingmin Gu,Meng Lv,Zhimin Guo,Guangmou Yan,Ling Yu,Chongtao Du,Xin Feng,Wenyu Han,Changjiang Sun,Liancheng Lei 한국미생물학회 2017 The journal of microbiology Vol.55 No.5
Bacteriophage endolysin is one of the most promising antibioticsubstitutes, but in Gram-negative bacteria, the outermembrane prevents the lysin from hydrolyzing peptidoglycansand blocks the development of lysin applications. Theprime strategy for new antibiotic substitutes is allowing lysinto access the peptidoglycan from outside of the bacteria byreformation of the lysin. In this study, the novel Escherichiacoli (E. coli) phage lyase lysep3, which lacks outside-in catalyticability, was fused with the N-terminal region of theBacillus amyloliquefaciens lysin including its cell wall bindingdomain D8 through the best manner of protein fusionbased on the predicted tertiary structure of lysep3-D8 to obtainan engineered lysin that can lyse bacteria from the outside. Our results showed that lysep3-D8 could lyse both Gramnegativeand Gram-positive bacteria, whereas lysep3 and D8have no impact on bacterial growth. The MIC of lysep3-D8on E. coli CVCC1418 is 60 μg/ml; lysep3-D8 can inhibit thegrowth of bacteria up to 12 h at this concentration. The bactericidalspectrum of lysep3-D8 is broad, as it can lyse of allof 14 E. coli strains, 3 P. aeruginosa strains, 1 Acinetobacterbaumannii strain, and 1 Streptococcus strain. Lysep3-D8 hassufficient bactericidal effects on the 14 E. coli strains testedat the concentration of 100 μg/ml. The cell wall binding domainof the engineered lysin can destroy the integrity of theouter membrane of bacteria, thus allowing the catalytic domainto reach its target, peptidoglycan, to lyse the bacteria. Lysep3-D8 can be used as a preservative in fodder to benefitthe health of animals. The method we used here proved to bea successful exploration of the reformation of phage lysin.
Shuang Wang,Qian Yang,Zhi-Hua Liu,Lei Sun,Dan Wei,Jun-Zheng Zhang,Jin-Zhu Song,Yun Wang,Jia Song,Jin-Xia Fan,Xian-Xin Meng,Wei Zhang 한국미생물학회 2011 The journal of microbiology Vol.49 No.1
A moderately halophilic bacterial strain 15-13^T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13^T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13^T were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13^T clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13^T was Halomonas pantelleriensis DSM 9661^T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661^T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13^T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13^T (=CGMCC 1.9129^T =NBRC 106539^T).
Shuang Wang,XingYao Liu,Yun Liu 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.5
Background: The treatment of acute kidney injury (AKI), a common disease in dogs, is limited. Therefore, an effective method to prevent AKI in veterinary clinics is particularly crucial. Objectives: Hydrogen sulfide (H2S) is the third gaseous signal molecule involved in various physiological functions of the body. The present study investigated the effect of H2S on cisplatin-induced AKI and the involved mechanisms in dogs. Methods: Cisplatin-injected dogs developed AKI symptoms as indicated by renal dysfunction and pathological changes. In the H2S-treated group, 50 mM sodium hydrosulfide (NaHS) solution was injected at 1 mg/kg/h for 30 min before cisplatin injection. After 72 h, tissue and blood samples were collected immediately. We performed biochemical tests, optical microscopy studies, analysis with test kits, quantitative reverse-transcription polymerase chain reaction, and western blot analysis. Results: The study results demonstrated that cisplatin injection increased necroptosis and regulated the corresponding protein expression of receptor interacting protein kinase (RIPK) 1, RIPK3, and poly ADP-ribose polymerase 1; furthermore, it activated the expressions of inflammatory factors, including tumor necrosis factor-alpha, nuclear factor kappa B, and interleukin-1β, in canine kidney tissues. Moreover, cisplatin triggered oxidative stress and affected energy metabolism. Conversely, an injection of NaHS solution considerably reduced the aforementioned changes. Conclusions: In conclusion, H2S protects the kidney from cisplatin-induced AKI through the mitigation of necroptosis and inflammation. These findings provide new and valuable clues for the treatment of canine AKI and are of great significance for AKI prevention in veterinary clinics.