Radiotherapy combined with immunotherapy could improve the immune infiltration of melanoma in mice and enhance the abscopal effect

Yufeng Zheng(Yufeng Zheng),Xue Liu(Xue Liu),Na Li(Na Li),Aimei Zhao(Aimei Zhao),Zhiqiang Sun(Zhiqiang Sun),Meihua Wang(Meihua Wang),Judong Luo(Judong Luo) 대한방사선종양학회 2023 Radiation Oncology Journal Vol.41 No.2

Purpose: To analyze the gene mutation, immune infiltration and tumor growth of primary tumor and distant tumor under different treatment modes. Materials and Methods: Twenty B16 murine melanoma cells were injected subcutaneously into the of both sides of the thigh, simulating a primary tumor and a secondary tumor impacted by the abscopal effect, respectively. They were divided into blank control group, immunotherapy group, radiotherapy group, and radiotherapy combined immunotherapy group. During this period, tumor volume was measured, and RNA sequencing was performed on tumor samples after the test. R software was used to analyze differentially expressed genes, functional enrichment, and immune infiltration. Results: We found that any treatment mode could cause changes in differentially expressed genes, especially the combination treatment. The different therapeutic effects might be caused by gene expression. In addition, the proportions of infiltrating immune cells in the irradiated and abscopal tumors were different. In the combination treatment group, T-cell infiltration in the irradiated site was the most obvious. In the immunotherapy group, CD8+ T-cell infiltration in the abscopal tumor site was obvious, but immunotherapy alone might have a poor prognosis. Whether the irradiated or abscopal tumor was evaluated, radiotherapy combined with anti-programmed cell death protein 1 (anti-PD-1) therapy produced the most obvious tumor control and might have a positive impact on prognosis. Conclusion: Combination therapy not only improves the immune microenvironment but may also have a positive impact on prognosis.

Three-Dimensional Lock and Key Colloids

Wang, Yu,Wang, Yufeng,Zheng, Xiaolong,Yi, Gi-Ra,Sacanna, Stefano,Pine, David J.,Weck, Marcus American Chemical Society 2014 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.136 No.19

<P>Colloids with well-defined multicavities are synthesized through the hydrolytic removal of silica cluster templates from organo-silica hybrid patchy particles. The geometry of the cavities stems from the originally assembled cluster templates, displaying well-defined three-dimensional symmetries, ranging from spherical, linear, triangular, tetrahedral, trigonal dipyramidal, octahedral, to pentagonal dipyramidal. The concave surface of the cavities is smooth, and the cavity shallowness and size can be varied. These particles with multicavities can act as “lock” particles with multiple “key holes”. Up to <I>n</I> “key” particles can self-assemble into the lock particles via depletion interaction, resulting in multivalent, site-specific, reversible, and flexible bonding.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2014/jacsat.2014.136.issue-19/ja502699p/production/images/medium/ja-2014-02699p_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja502699p'>ACS Electronic Supporting Info</A></P>

Optimization of coagulation–flocculation process for papermaking-reconstituted tobacco slice wastewater treatment using response surface methodology

Yufeng Wang,Lihuan Mo,Kefu Chen,Jun Li,Jun Xu 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.2

A coagulation–flocculation process was used to treat papermaking-reconstituted tobacco slicewastewater with polyaluminium chloride (PAC) as coagulant and a cationic polyacrylamide (CPAM)as flocculant. To maximize the reduction of chemical oxygen demand (COD) and color, the jar tests werecarried out in the experiments and response surface methodology (RSM) was applied to optimize theprocess. A central composite design, i.e., a standard approach in RSM, was used to evaluate the effectsand interactions of three factors, i.e. PAC dosage, CPAM dosage and pH on the treatment efficiency. Results revealed that the maximum reduction of COD and color could be achieved at an optimalconditions, i.e., PAC = 715 mg/L, CPAM = 4.8 mg/L and pH = 6.6, from which the reduction of COD andcolor were 67.8% and 77.7%, respectively. The study also showed that the regression equations could beused as the theoretical basis for coagulation–flocculation process of papermaking-reconstituted tobaccoslice wastewater. They will be very helpful to flexibly select the appropriate process parameters in theengineering applications

Synthetic Strategies Toward DNA-Coated Colloids that Crystallize

Wang, Yufeng,Wang, Yu,Zheng, Xiaolong,Ducrot, É,tienne,Lee, Myung-Goo,Yi, Gi-Ra,Weck, Marcus,Pine, David J. American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.33

Security Authentication Method of Terminal Trusted Access in Smart Grid

Yufeng Wang,Lie Wu,Yun Yang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.7

A security authentication method in smart grid terminal is proposed in this paper. This method layers the terminal authentication system structure of smart grid so as to improve the simplicity and extensibility on system deployment and realize flexible communication mechanism and the interaction mechanism among systems. This paper aims at preventing terminal information from being destroyed and achieving terminal verification integrity. Besides, this kind of authentication method has no special requirements for terminal users.

Throughput and Delay of Single-Hop and Two-Hop Aeronautical Communication Networks

Yufeng Wang,Mustafa Cenk Ertürk,Jinxing Liu,나인호,산카,Salvatore Morgera 한국통신학회 2015 Journal of communications and networks Vol.17 No.1

Aeronautical communication networks (ACN) is an emerging concept in which aeronautical stations (AS) are considered as a part of multi-tier network for the future wireless communication system. An AS could be a commercial plane, helicopter, or any other low orbit station, i.e., Unmanned air vehicle, high altitude platform. The goal of ACN is to provide high throughput and cost effective communication network for aeronautical applications (i.e., Air traffic control (ATC), air traffic management (ATM) communications, and commercial in-flight Internet activities), and terrestrial networks by using aeronautical platforms as a backbone. In this paper, we investigate the issues about connectivity, throughput, and delay in ACN. First, topology of ACN is presented as a simple mobile ad hoc network and connectivity analysis is provided. Then, by using information obtained fromconnectivity analysis, we investigate two communication models, i.e., single-hop and two-hop, in which each source AS is communicating with its destination AS with or without the help of intermediate relay AS, respectively. In our throughput analysis, we use the method of finding the maximum number of concurrent successful transmissions to derive ACN throughput upper bounds for the two communication models. We conclude that the two-hop model achieves greater throughput scaling than the single-hop model for ACN and multihop models cannot achieve better throughput scaling than two-hop model. Furthermore, since delay issue is more salient in two-hop communication, we characterize the delay performance and derive the closed-form average end-to-end delay for the two-hop model. Finally, computer simulations are performed and it is shown that ACN is robust in terms of throughput and delay performances.

The Fabrication of High-Aspect-Ratio Cylindrical Nano Tool using ECM

Yufeng Wang,Ningsong Qu,Yongbin Zeng,Xiujuan Wu,Di Zhu 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

With the miniaturization of various types of components and devices, micro- and nanometer-scale tools are increasingly in demand. Electrochemical machining (ECM) is a well-established technique for the fabrication of micro tools and nano tips. This paper presents a machining method for the fabrication of a high-aspect-ratio cylindrical nano tool using liquid membrane ECM, in which a straight reciprocating motion is applied to the anodic electrode. The method is based on periodic machining of the anodic rod within the amplitude of the straight reciprocating motion. In this process, the machining rate within amplitude varies periodically, allowing nano tools with high aspect ratios to be obtained by the presented method. Experiments were conducted to verify the proposed method. The influences of applied voltage, electrolyte concentration, amplitude and velocity of the straight reciprocating motion on the fabrication of cylindrical nano-electrode were investigated. By using this method, nano-electrodes with average diameters of several hundred nanometers and aspect ratios up to 70 were successfully fabricated.

Active Patchy Colloids with Shape-Tunable Dynamics

Wang, Zuochen,Wang, Zhisheng,Li, Jiahui,Cheung, Simon Tsz Hang,Tian, Changhao,Kim, Shin-Hyun,Yi, Gi-Ra,Ducrot, Etienne,Wang, Yufeng American Chemical Society 2019 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.141 No.37

<P>Controlling the complex dynamics of active colloids-the autonomous locomotion of colloidal particles and their spontaneous assembly-is challenging yet crucial for creating functional, out-of-equilibrium colloidal systems potentially useful for nano- and micromachines. Herein, by introducing the synthesis of active “patchy” colloids of various low-symmetry shapes, we demonstrate that the dynamics of such systems can be precisely tuned. The low-symmetry patchy colloids are made in bulk via a cluster-encapsulation-dewetting method. They carry essential information encoded in their shapes (particle geometry, number, size, and configurations of surface patches, etc.) that programs their locomotive and assembling behaviors. Under AC electric field, we show that the velocity of particle propulsion and the ability to brake and steer can be modulated by having two asymmetrical patches with various bending angles. The assembly of monopatch particles leads to the formation of dynamic and reconfigurable structures such as spinners and “cooperative swimmers” depending on the particle’s aspect ratios. A particle with two patches of different sizes allows for “directional bonding”, a concept popular in static assemblies but rare in dynamic ones. With the capability to make tunable and complex shapes, we anticipate the discovery of a diverse range of new dynamics and structures when other external stimuli (e.g., magnetic, optical, chemical, etc.) are employed and spark synergy with shapes.</P> [FIG OMISSION]</BR>

Comparative transcriptome analysis of salt-sensitive and salt-tolerant maize reveals potential mechanisms to enhance salt resistance

Mingquan Wang,Yufeng Wang,Yifei Zhang,Chunxia Li,Shichen Gong,Shuqin Yan,Guoliang Li,Guanghui Hu,Honglei Ren,Jianfei Yang,Tao Yu,Kejun Yang 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background Salt stress is a devastating environmental stress that causes plant growth inhibition and yield reduction. Objective The identification of salt-tolerant genes brings hope for the generation of salinity-tolerant crop plants through molecular breeding. Methods In this study, one salt-sensitive and one salt-tolerant maize inbred line were screened from 242 maize inbred lines. Reactive oxygen species (ROS)-related enzyme activities were detected and salt-responsive comparative transcriptome analysis was performed for control and 220 mM NaCl treated maize leaves. Results Salt-tolerant maize inbred line (L87) showed higher ROS-related enzyme (SOD, POD, APX and CAT) activities and accumulated relatively lower levels of ROS under salt stress. Of the total DEGs, 1856 upregulated DEGs were specific to L87, including stress tolerance-related members of the 70kDa family of heat shock proteins (Hsp70s) and aquaporins. The DEGs involved in the abscisic acid (ABA), ethylene, jasmonic acid (JA) and salicylic acid (SA) signal transduction pathways may determine the difference in salt tolerance between the two varieties, especially one central component SnRK2, that positively regulates ABA signaling and was only upregulated in L87. Analysis of DEGs related to ROS scavenging showed that some peroxidase (POD), glutathione S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) genes specific to L87 probably enhanced its salt tolerance. The analysis of differentially expressed transcription factors (TFs) suggested that WRKY TFs could contribute to the difference in salt tolerance between the two maize lines. Conclusion Compared with Salt-sensitive maize inbred line (L29), L87 exhibits specific regulatory mechanisms related to salt tolerance, including plant hormone interactions, ROS scavenging and the regulation of TFs. Our study identifies new candidate genes that may regulate maize tolerance to salt stress and provides useful information for breeding maize with high salt resistance.

Contact characteristics and interfacial motion states during sphere oblique impact with initial spin

Qingpeng Wang,Kai Zhang,Zhenfeng Wang,Heng Wang,Yufeng Sun,Xiankun Gao,Guangyin Xu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.5

The impact between a sphere and flat surface plays a significant role in the granular handling process. A method is proposed to investigate the evolution of interfacial states for the sphere oblique impact with initial spin. Firstly, the sphere with irregular surface structures is applied, and the imprinted surfaces are recorded with a thin carbon paper. Then the interfacial characteristics are analyzed in cases of impact angle, contact material and drop height, etc. The geometrical parameters decrease exponentially with impact angle and include three main stages: plateau, slow decrease and sharp decrease. The motional ratio corresponds to slow increase, steady state and sharp increase. For the no pre-spin, it goes through five interfacial states with impact angle. Comparatively, the forward spin decreases the critical angle, and the backward spin decreases the number of states. Furthermore, the parameters increase linearly with drop height, while the effect on interfacial states is very little.