충청 서북부 심혈관 질환자의 항산화 영양소 섭취정도 분석

김희선,임혜성 순천향대학교 기초과학연구소 2000 순천향자연과학연구 논문집 Vol.6 No.1

Dietary intakes of vitamin A and C were studied with cardiovascular patients in Chungnam area. Seventy five out-patients(63.3±9.07 years old) with myocardial infarction(MI) history of more than 3 month past were selected and all subjects were gathered at once for cardiac rehabilitation program. Twenty four hour recall was conducted to investigate amounts of dietary vitamin A and C intakes by trained interviewers. The results showed that calorie intake was lower than recommended level for most patients while huge variation was observed in the amount of dietary vitamin A intake. Dietary vitamin C intake was adequate for most patients. When gender comparison was conducted, female patients showed much lower calorie and vitamin A intakes compared to male patients. As age increased, vitamin A intake was decreased while calorie and vitamin C intake did not show any differences. In conclusion, vitamin A and C intakes as well as calorie intake of MI patients in Chungnam area was not adequate. The present data suggested that proper nutritional advice was essential for rehabilitation of MI patients and to improve Patients' health.

대전지역 대학생의 학교 식당 이용실태 및 만족도 조사

왕수경,임영희,윤은영,구난숙,박선미 대전대학교 생활과학연구소 2000 생활과학연구 Vol.6 No.-

The aspects and satisfaction on university foodservice were investigated to provide basic information for better foodservice in university by questionnaires. The survey was conducted in Daejon, and data were analyzed by SAS. Although 87.3% of subjects were under foodservice, only 2.0% of them preferred the foods served in university. The most favorite reason for foodservice was indicated as no need on lunch bag(31.1%) and frendship of classmates(25.5%). The most unfavorite reason for foodservice was indicated as bad taste of foods(68.7%) or many dislike foods(13.2%), also dirty eating place(7.7%). The subject liked ssalbap, baechu kimchi, beef foods. The acceptance about kongbap, yulmu kirnchi, beef products was higher in men than women(p<0.05). The preference of mieokkuk was higher in women and that of beef soup was higher in men(p<0.05). The degree of satisfaction was lower in men than in women about the amount of foods(p<0.001). Also the degree of satisfaction was lower in women than in men about sanitary condition of eating place. 54.6% of them had taken the unwanted materials in foods and 3% of them experienced the deteriorated food. The results suggest that food preference of subjects should be considered in meal planning and various cooking method should be utilized to enhance taste of foods for better foodservice. Also, the importance of various foods intake must be periodically and continuously taught to them through nutrition education.

2,4-D 處理가 'Harcot' 살구와 '美白' 복숭아의 果實 成熟과 品質에 미치는 影響

윤철구,김영호,임상철,김학현,이철희,최관순,김선규 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-

Effect of 2,4- D application on the fruit maturity and quality of 'Harcot' apricot and 'Mibaek' peach was examined. Leaf area of 'Harcot' apricot was not affected by 2,4-D while that of 'Mibaek' peach was increased by 35mg · L-1 2,4-D. Application of 2,4-D at 35mgㆍL-1 increased the fruit weight of both species, and that of 'Harcot' was doubled. Soluble solids content of 'Harcot' was decreased by 2,4-D while that of 'Mibaek' was not affected. Fruit maturity of 'Harcot' and 'Mibaek' was enhanced for respective 4 and 1 day by 2,4-D application. Percent fruit cracking of 'Harcot' apricot was decreased by 35mg · L-1 2,4-D.

지베렐린과 에세폰의 葉面撒布가 '美白' 복숭아의 熟期와 品質에 미치는 影響

김영호,윤철구,임상철,김학현,이철희,최관순,김선규 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-

In order to obtain the basic data for artificial maturity control of 'Mibaek' peach, GA and ethephon were foliar sprayed 4 weeks prior to harvest, and their effects on fruit maturity and quality were examined. GA and ethephon tended to decrease the fruit weight except 100mg ㆍ L-1 GA+50 to 100mg · L-1 ethephon treatment. GA increased the fruit firmness, and ethephon at higher concentration increased the fruit drop. Harvest date was advanced for 7 to 8 days by ethephon while was delayed for 6 to 8 days by GA treatment, regardless of concentration.

GA 도포제와 염화칼슘 처리시기가 ‘신고’ 과실의 성숙 및 저장성에 미치는 영향

윤철구(Cheol Ku Youn),김선규(Seon Kyu Kim),임상철(Sang Cheol Lim),김학현(Hag Hyon Kim),김영호(Young Ho Kim),이철희(Cheol Hee1 Lee),최관순(Kwan Soon Choi) 한국원예학회 2000 원예과학기술지 Vol.18 No.2

Complementation of PAT Gene in the Tryptophan Mutants of Arabidopsis thaliana

Lim, Seon-Hee,Cheong, Hyeonsook 조선대학교 기초과학연구소 1997 自然科學硏究 Vol.20 No.1

In Arabidopsis thaliana, mutants are currently being used as tools for studing plant development and for examining metabolic pathway. Phosphoribosylanthranilate transferase (PAT) catalyzes the second step of the tryptophan biosynthetic pathway and is encoded by a single-copy gene that complements all the visible phenotypes of the tryptophan mutant (trp1-100) of Arabidopsis. The trp1-100 is blue fluorescent under UV light because it accumulates anthranilate. To obtain a plant with reducing PAT activity, PAT1 genes with several internal deletions in different promoter regions were introduced into trp1-100 using Agrobacterium. Then, homozygous T_3 plants were isolated and examined for blue fluorescence. Introduction of the PAT1 gene fusants results in the reversion of fluorescence phenotype except in the case of pHS105. A plant line carrying pHS105 gene fusant dose not completely complement the blue fluorescence but, it accumulates less anthranilate than trp1-100. The activity of PAT was reduced in the transgenic mutant as well.

Graphene oxide induces apoptotic cell death in endothelial cells by activating autophagy via calcium-dependent phosphorylation of c-Jun N-terminal kinases

Lim, Mi-Hee,Jeung, In Cheul,Jeong, Jinyoung,Yoon, Sung-Jin,Lee, Sang-Hyun,Park, Jongjin,Kang, Yu-Seon,Lee, Hansu,Park, Young-Jun,Lee, Hee Gu,Lee, Seon-Jin,Han, Baek Soo,Song, Nam Woong,Lee, Sang Chul Elsevier 2016 Acta Biomaterialia: structure-property-function re Vol.46 No.-

<P><B>Abstract</B></P> <P>Despite the rapid expansion of the biomedical applications of graphene oxide (GO), safety issues related to GO, particularly with regard to its effects on vascular endothelial cells (ECs), have been poorly evaluated. To explore possible GO-mediated vasculature cytotoxicity and determine lateral GO size relevance, we constructed four types of GO: micrometer-sized GO (MGO; 1089.9±135.3nm), submicrometer-sized GO (SGO; 390.2±51.4nm), nanometer-sized GO (NGO; 65.5±16.3nm), and graphene quantum dots (GQDs). All types but GQD showed a significant decrease in cellular viability in a dose-dependent manner. Notably, SGO or NGO, but not MGO, potently induced apoptosis while causing no detectable necrosis. Subsequently, SGO or NGO markedly induced autophagy through a process dependent on the c-Jun N-terminal kinase (JNK)-mediated phosphorylation of B-cell lymphoma 2 (Bcl-2), leading to the dissociation of Beclin-1 from the Beclin-1–Bcl-2 complex. Autophagy suppression attenuated the SGO- or NGO-induced apoptotic cell death of ECs, suggesting that SGO- or NGO-induced cytotoxicity is associated with autophagy. Moreover, SGO or NGO significantly induced increased intracellular calcium ion (Ca<SUP>2+</SUP>) levels. Intracellular Ca<SUP>2+</SUP> chelation with BAPTA-AM significantly attenuated microtubule-associated protein 1A/1B-light chain 3-II accumulation and JNK phosphorylation, resulting in reduced autophagy. Furthermore, we found that SGO or NGO induced Ca<SUP>2+</SUP> release from the endoplasmic reticulum through the PLC β3/IP<SUB>3</SUB>/IP<SUB>3</SUB>R signaling axis. These results elucidate the mechanism underlying the size-dependent cytotoxicity of GOs in the vasculature and may facilitate the development of a safer biomedical application of GOs.</P> <P><B>Statement of Significance</B></P> <P>Graphene oxide (GO) have received considerable attention with respect to their utilization in biomedical applications. However, GO-related safety issues concerning human vasculature are very limited. In this manuscript, we report for the first time the differential size-related biological effects of GOs on endothelial cells (ECs). Notably, Subnanometer- and nanometersized GOs induce apoptotic death in ECs via autophagy activation. We propose a molecular mechanism for the GO-induced autophagic cell death through the PLCβ3/IP3/Ca<SUP>2+</SUP>/JNK signaling axis. Our findings could be provide a better understanding of the GO sizedependent cytotoxicity in vasculature and facilitate the future development of safer biomedical applications of GOs.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Nutritional Status and Dietary Management According to Hemodialysis Duration

( Hee-sook Lim ),( Hee-seon Kim ),( Jin Kuk Kim ),( Mooyong Park ),( Soo Jeong Choi ) 한국임상영양학회 2019 Clinical Nutrition Research Vol.8 No.1

As the incidence of chronic diseases such as diabetes and hypertension increases, complications such as decreased renal function are also increasing in many patients. Nutritional management in hemodialysis patients is a very important factor for prognosis and quality of life. The purpose of this study was to investigate the differences in nutritional status and dietary management according to hemodialysis duration. A total of 145 patients were divided into 4 groups according to hemodialysis duration: less 1 year (D1), 1-5 years (D2), and above 5 years (D3). The rates of protein-energy wasting were 31.1% in D1 group, 49.5% in D2 group, and 47.6% in D3 group. However, there was no significant difference between the 3 groups. Nutrient intake analysis showed that protein, iron, and vitamin C were significantly lower in the D3 group than in the D1 group. Protein intake in all 3 groups was insufficient compared to the recommended dietary amount for dialysis patients. The most difficult aspect in dietary management was cooking with low sodium. In the D3 group, which had the longest duration of dialysis, the practice of diet therapy and self-perceived need for nutrition education was lowest. Observations of nutritional status are necessary to maintain the health status of dialysis patients. In addition, education plans should be prepared to mediate the nutrient intakes and identify the patient's difficulties and provide practical help.

Blood pressure measurements and hypertension in infants, children, and adolescents: from the postmercury to mobile devices

Lim Seon Hee,Kim Seong Heon 대한소아청소년과학회 2022 Clinical and Experimental Pediatrics (CEP) Vol.65 No.2

A mercury sphygmomanometer (MS) has been the gold standard for pediatric blood pressure (BP) measurements, and diagnosing hypertension is critical. However, because of environmental issues, other alternatives are needed. Noninvasive BP measurement devices are largely divided into auscultatory and oscillometric types. The aneroid sphygmomanometer, the currently used auscultatory method, is inferior to MS in terms of limitations such as validation and regular calibration and difficult to apply to infants, in whom Korotkoff sounds are not audible. The oscillometric method uses an automatic device that eliminates errors caused by human observers and has the advantage of being easy to use; however, owing to its measurement accuracy issues, the development of an international validation protocol for children is important. The hybrid method, which combines the auscultatory and electronic methods, solves some of these problems by eliminating the observer bias of terminal digit preference while maintaining measurement accuracy; however, the auscultatory method remains limited. As the age-related characteristics of the pediatric group are heterogeneous, it is necessary to reconsider the appropriate BP measurement method suitable for this indication. In addition, the mobile application-based BP measurement market is growing rapidly with the development of smartphone applications. Although more research is still needed on their accuracy, many experts expect that mobile application-based BP measurement will effectively reduce medical costs due to increased ease of access and early BP management.

Sarcopenic Obesity: Prevalence and Association With Metabolic Syndrome in the Korean Longitudinal Study on Health and Aging (KLoSHA)

Lim, Soo,Kim, Jung Hee,Yoon, Ji Won,Kang, Seon Mee,Choi, Sung Hee,Park, Young Joo,Kim, Ki Woong,Lim, Jae Young,Park, Kyong Soo,Jang, Hak Chul American Diabetes Association 2010 Diabetes care Vol.33 No.7

<P><B>OBJECTIVE</B></P><P>We investigated the prevalence of sarcopenic obesity (SO) and its relationship with metabolic syndrome in a community-based elderly cohort in Korea.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>In this study, 287 men and 278 women aged 65 or older were recruited. Sarcopenia was defined as the appendicular skeletal muscle mass (ASM) divided by height squared (Ht<SUP>2</SUP>) (kg/m<SUP>2</SUP>) or by weight (Wt) (%) of <1 SD below the sex-specific mean for young adults. Obesity was defined as a visceral fat area ≥100 cm<SUP>2</SUP>.</P><P><B>RESULTS</B></P><P>The prevalence of SO was 16.7% in men and 5.7% in women with sarcopenia defined by ASM/Ht<SUP>2</SUP>; however, it was 35.1% in men and 48.1% in women by ASM/Wt. Using ASM/Wt, the homeostasis model assessment of insulin resistance of subjects with SO was higher and they were at higher risk for metabolic syndrome (odds ratio [OR] 8.28 [95% CI 4.45–15.40]) than the obese (5.51 [2.81–10.80]) or sarcopenic group (2.64 [1.08–6.44]).</P><P><B>CONCLUSIONS</B></P><P>SO defined by ASM/Wt was more closely associated with metabolic syndrome than either sarcopenia or obesity alone.</P>