선모(仙茅) 열수(熱水) 추출물의 Collagen 유발 관절염에 대한 약리 효능 연구

서부일 ( Bu Il Seo ),노성수 ( Seong Soo Roh ),박지하 ( Ji Ha Park ),박찬익 ( Chan Ik Park ),구진숙 ( Jin Suk Koo ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.4

Objectives:In Korean medicine, Curculiginis Rhizoma was treated for arthritis in remedy. But efficacy of Curculiginis Rhizoma on collagen induced arthritis was not revealed. Methods:Anti inflammatory effect of Curculiginis Rhizoma was researched in vitro with RAW264.7 cell and cell toxicity, levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-12) and PGE2 were analyzed by ELISA assay. Inflammatory protein were analyzed by western blotting assay (JNK, ERK, COX-2, TNF-α and IL-1β). In vivo, collagen induced arthritis mice model was used to evaluate anti-inflammation effect through arthritis index, immune cell number and cytokine levels (TNF-α, IL-6 and IL-1β) in serum. Results:ECR(Extract of Curculiginis Rhizoma) has not shown cell toxicity in 200 ㎍/㎖ on RAW264.7 cell. ECR suppressed releases of NO, TNF-α, IL-1β, IL-6, IL-12 and PGE2 on RAW264.7 cell treated with lipopolysacharide (1 ㎍/㎖). And ECR inhibited regulation of TNF-α, IL-1β and IL-6 mRNA, reduced protein release of JNK, ERK, iNOS, COX-2, IL-1β and TNF-α. AI of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly decreased compared to vihicle arthritis mice, the number of immune cell in foot joint was increased on control mice but those of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly reduced. This results correspond with contens of cytokines (TNF-α, IL-1β and IL-6) in serum. Conclusions:Curculiginis Rhizoma has anti-inflammation effect on RAW264.7 cell in vitro and collagen induced arthritis in vivo . So it is necessary to research more mechanism for cascade imfact.

Association of DOCK8, IL17RA, and KLK12 Polymorphisms with Atopic Dermatitis in Koreans

( Won Il Heo ),( Kui Young Park ),( Mi-kyung Lee ),( Yu Jeong Bae ),( Nam Ju Moon ),( Seong Jun Seo ) 대한피부과학회 2020 Annals of Dermatology Vol.32 No.3

Background: Early-onset and severe atopic dermatitis (AD) in patients increase the probability of the development of allergic rhinitis or asthma. Treatment and prevention strategies in infants and young children with AD are targeted toward treating the symptoms, restoring skin barrier functions, and reducing the absorption of environmental allergens in an attempt to attenuate or block the onset of asthma and food allergy. Objective: Given that the initiating events in AD remain poorly understood, identifying those at risk and implementing strategies to prevent AD is necessary. Methods: Whole-exome sequencing (WES) was performed in a 43 control group and a disease group with 20 AD patients without atopic march (AM) and 20 with AM. Sanger sequencing was carried out to validate found variants in cohorts. Results: DOCK8, IL17RA, and KLK12 single-nucleotide polymorphisms were identified by WES as missense mutations: c.1289C>A, p.P97T (rs529208); c.1685C>A, p.P562G (rs12484684); and c.457+27>C, rs3745540, respectively. A case-control study show that total immunoglobulin E (IgE) level was significantly increased in the AA genotype of DOCK8 compared to the CA genotype in allergic patients. The rs12484684 of IL17RA increased risk of adult-onset AD (odds ratio: 1.63) compared to the control for (A) allele frequency. AD and AM Patients with the IL17RA CA genotype also had elevated IgE levels. rs3745540 of KLK12 was associated with AD in dominant model (odds ratio: 2.86). Conclusion: DOCK8 (rs529208), IL17RA (rs12484684), and KLK12 (rs3745540), were identified using a new WES filtering method. the result suggests that polymorphism of DOCK8 and IL17RA might be related to increase the total IgE level. (Ann Dermatol 32(3) 197∼205, 2020)

Regulation of interleukin-11 expression in ovulatory follicles of the rat ovary

Jang, You-Jee,Park, Jae-Il,Jeong, Seong-Eun,Seo, You-Mi,Dam, Phuong T. M.,Seo, Young-Woo,Choi, Bum-Chae,Song, Sang-Jin,Chun, Sang-Young,Cho, Moon-Kyoung Commonwealth Scientific and Industrial Research Or 2017 Reproduction, fertility, and development Vol. No.

<P> The aim of the present study was to examine the regulation of interleukin (IL)-11 expression, as well as the role of IL-11, during ovulation in gonadotropin-primed immature rats. Injection of equine chorionic gonadotropin (eCG), followed by human CG (hCG) to induce superovulation stimulated expression of the Il11 gene in theca cells within 6 h, as revealed by northern blot and in situ hybridisation analyses. Real-time reverse transcription-polymerase chain reaction analysis showed that the IL-11 receptor, α subunit gene was expressed in granulosa and theca cells and that injection of hCG had no effect on its expression. IL-11 protein expression was stimulated in theca cells by hCG. LH-stimulated increases in Il11 mRNA levels in cultured preovulatory follicles were inhibited by protein kinase A and mitogen-activated protein kinase kinase inhibitors. Toll-like receptor (TLR) 2 and TLR4 were detected in preovulatory follicles, and the TLR4 ligand lipopolysaccharide, but not the TLR2 ligand Pam3Cys, increased Il11 mRNA levels in theca cells, but not in granulosa cells. Treatment of preovulatory follicles with IL-11 stimulated progesterone production and steroidogenic acute regulatory protein (Star) gene expression. Together, these results indicate that IL-11 in theca cells is stimulated by mitogen-activated protein kinase signalling and TLR4 activation, and increases progesterone production during ovulation. </P>

사람의 자궁 내막 조직내에서 Phosphodiesterase IV Inhibitor에 의한 IL-12의 조절 및 이에 따른 Th-1, Th-2 cytokine 분비 양상의 변화

박원일 ( Park Won Il ),김은경 ( Kim Eun Gyeong ),고덕성 ( Go Deog Seong ),홍서유 ( Hong Seo Yu ),나중열 ( Na Jung Yeol ),김대운 ( Kim Dae Un ),신정환 ( Sin Jeong Hwan ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.8

목적 : 사람의 초기 임신 과정에서 phosphodiesterase type IV inhibitor인 rolipram이 탈락막내 IL-12를 억제하고 이에 따라 Th-1 계열의 cytokine이 감소하고 Th-2 cytokine이 증가하는지를 규명하는 것이 목적이다. 연구 방법 : 임신 12주 이전에 계류 유산으로 진단받은 10명과 정상임신에서 임신 중절 수술을 시행받은 10명에서 자궁 소파술을 통하여 탈락막 조직을 획득한 후 조직을 rolipram으로 Objective : To assess the capability of phosphodiesterase type IV inhibitor (rolipram) to suppress IL-12 in human decidua and the subsequent changes of Th-2 cytokine (IL-10) and Th-1 cytokine (TNF-α). Methods : Decidual tissues of 10 first-trimester pregn

The whole-exome sequencing in three families with atopic dermatitis

( Joon Hyuk Seo ),( Moo Yeol Hyun ),( Won Il Heo ),( Kapsok Li ),( Seong Joon Seo ),( Chang Kwun Hong ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: The prevalence of atopic dermatitis (AD) has increased over a recent 10-year. AD tends to run in families that passed down from generation to generation and commonly starts in childhood. It is as high as 20% in children less than 5 years old. Thus, early discovery and therapy of AD are important. It has emerged the necessity of biomarker for early detection of AD. Objectives: The goal of this study is to find novel gene markers for diagnosis of early-onset atopic dermatitis in Koreans. Methods: Peripheral blood was obtained from three families (6 early-onset AD patients and 6 controls) with autosomal dominant condition. Whole Exome Sequencing (WES) was performed using SureSelect Human All Exon V4+UTR 71 Mb. Variants of atopic dermatitis were filtered step by step to gain the important candidate genes. Results: We have confirmed overlapping genes in common variants of filter 5 in three family. 15 genes were overlapped in filter 5 and two of them reached to filter 7 can be called “rare variant”. Uniquely, COL6A6 gene appeared in all of three family. We also found variants of filaggrin, filaggrin2 and IL4R that is well-known makers of atopic dermatitis. Conclusion: We were able to detect COL6A6 gene by finding overlapping genes in rare and common variants of three family. It may become a novel gene as early-onset AD marker.

Genetic polymorphism of DOCK8, IL17RA and KLK12 in Koreans with atopic dermatitis and allergic march

( Hye Sung Han ),( Jae Min Kim ),( Won Il Heo ),( Kui Young Park ),( Mi-kyung Lee ),( Seong Jun Seo ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Atopic dermatitis (AD) is one of the most common childhood diseases and has a complex etiology involving genetic and environmental factors. Thus, a broad understanding of genetic background is needed for early diagnosis of AD. Objectives: The purpose of this study was to identify of novel candidate functional genetic variants in Koreans with atopic dermatitis and allergic march. Methods: Whole-exome sequencing (WES) was performed in 20 AD and 20 AM Korean patients, and 40 Korean personal genome project (KPGP) controls. Sanger sequencing was carried out to validate found variants in 86 AD, 72 AM patients and 81 controls. Results: Three candidate variants of DOCK8 (rs529208), IL17RA (rs1248468), and KLK12 (rs3745540) were identified. rs529208 had no correlation with the development of AD but the AA genotype of DOCK8 had significantly increased total IgE level. rs1248468 had significantly increased risk of AD and the CA genotype of IL17RA had also increased total IgE level. rs3745540 was significantly associated with AD. Conclusion: DOCK8 (rs529208), IL17RA (rs1248468) and KLK12 (rs3745540) were deemed functionally interesting based on WES. Our case-control study suggests that the three variants detected in WES may enhance the risk of AD.

The Role of Collagen VI α6 Chain Gene in Atopic Dermatitis

( Hye Jung Jung ),( Won Il Heo ),( Kui Young Park ),( Mi-kyung Lee ),( Ji Young Ahn ),( Mi Youn Park ),( Seong Jun Seo ) 대한피부과학회 2022 Annals of Dermatology Vol.34 No.1

Background: In a previous study, we carried out whole-exome sequencing to identify genetic variants associated with early onset atopic dermatitis (AD) in Koreans and found that collagen VI α6 chain (COL6A6) gene polymorphisms are associated. COL6A6 is one of the chains that makes up the triple helix of collagen VI, and little is known about its role in AD. Objective: To identify how COL6A6 changes in AD and clarify its role. Methods: Immunohistochemical staining for COL6A6 was performed on tissues of AD, other skin diseases, and healthy controls. Human keratinocytes and fibroblasts were exposed to inflammatory cytokines and cultured to evaluate changes in COL6A6 expression. COL6A6 small interfering RNA (siRNA) was transfected into cells to identify the role of COL6A6. Results: Total COL6A6 mRNA was higher in AD than in controls. In AD tissues, COL6A6 mRNA decreased significantly in the epidermis compared to controls, whereas COL6A6 protein was increased in the dermis. In the cultured cells, COL6A6 mRNA was suppressed in the epidermis by interleukin (IL)-4 and IL-13, whereas COL6A6 protein was induced in the dermis. In the COL6A6 siRNA-transfected keratinocyte, mRNA of FLG, LOR, and CASP14 decreased compared to controls; in contrast, mRNA of MMP1 increased. Conclusion: The reduction of epidermal COL6A6 due to the genetic mutation can cause skin barrier damage and it can contributes to the early onset of AD. COL6A6 is induced by IL-4 and IL-13, and it may play a role in fibrotic remodeling and inflammatory processes, which are major features of AD.

소아 두뇌외상의 임상적 고찰

서성일,송시헌,김성호,김관태,김윤 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.2

The clinical analysis was carried out with 182 cases of craniocerebral injuries under 15 years of age, admitted at the Department of Neurosurgery, Chung Nam National Universty Hospital during the period from September, 1990 to September 1993. Following results were obtained : 1) There were 133 males and 49 females and sex ratio was 2.7% 2) Age incidence of the accidents was greatest at 6 years of age and mostly distributed to the age group between 4 to 7. 3) Seasonal peak of the accidents was at May. 4) The accident occurred mostly botween 4 P.m. and 6 P.m in a day.5) The most frequent cause of the craniocerebral injuries was traffic accident(105cases, 57.69%) 6) No deterioration of cansciousness before and after the admission was doserved in 68cases(37.36%) 7) Pupillary change was observed in 25 cases and Babinski sign was elicitable in 31cases 8) Early convulsion and vomiting were frequent symptoms in childhood. 9) Skull fracture was observed in 72 cases(39.56%) in which linear fracture was most frequent and predilection site was parietal bone. 10) The accompanying rate of skull fracture in intracranial hemorrhage was 65.54% 11) Associated injures were found in 44 cases(24.18%). The frequent ones were e xtremity fracture, clavicle fracture 12) The overall mortality was 7.14%.

개두술후 발생한 술후 출혈

김성호,서성일 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

Of 1713 patients who underwent craniotomy during previous 6years, 41(2.39%) developed postoperative hemorrhages. Postoperative hemorrhages occured most frequently after the operation for spontaneous intracerebral hematomas(8.2%). The most frequent site and type of postoperative hemorrhages were ipsilateral same space and acute epidural hematomas. Patients who developed postoperative hemorrhages had a significantly worse prognosis compared to those who did not(p=0.0071). Among the various causes, coagulopathy(8 cases) and sudden brain shifting(8 cases) were most frequent probable causes.

중등도 두부 외상에 대한 임상적 고찰

김윤,서성일 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

The present study is made to evaluate prognostic factors in 275 patients with moderate head injuries collected from entire 1105 head injured patients who were admitted to the Department of Neurosurgery, Chung Nam National University Hospital from January,1990 to September,1993. The results were as follows : 1) The severity rate of head trauma was as follows : 11.8 % in head trauma were severe (GCS 3-8), 24.8 % were moderate(GCS 9-12), 63.4% were mild( GCS 13-15). 2) The most common age group in moderate head injury was 3rd decade. 3) Males outnumbered females by almost 2.6 to 1. 4) The most common type injury in moderate head injuries was cerebral contusion followed by SDH. 5) The most common cause was motor vehicle accident followed by fall. 6) The most common combined injury was facial bone fracture followed by spinal injury. 7) In the overall outcome of 275 patients, 39 % made a good recovery, 32 % were moderately disabled, 13 % were left severely disabled, 2 % were vegetative, 14 % of the patients died. 8) 83 patients (32 %) were performed cranial operation under the diagnosis of EDH (21 patients), SDH (32 patients), ICH & DTICH (19 patients), IVH, contusion and FCCD or CSF leakage. 9) The mortality rate observed in patients operated for a cranial lesion was 17 % and the mortality rate in patients not undergoing surgery was 12.5%. The difference was statistically significant. 10) The period of hospitalization in moderate head injury was 4 weeks on an average.