Anti-inflammatory Effect of LFR on LPS-stimulated THP-1 Cells

정승기,Sul, Mu-Chang,Kim, Hong-Yeoul,Roh, Young-Lae,Jung, Hee-Jae,Jung, Sung-Ki 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.2

Background and Objective: Luffae Fructus Retinervus (LFR) is used for investigating symptoms of inflammation. We have evaluated the anti-inflammatory effect of LFR by analyzing the expression of pro-inflammatory cytokines. Materials and Methods : We differentiated THP-l cells into macrophage-like cells by treatment with PMA. Inflammation was induced by treatment with LPS and PMA. We determined the safe concentration of LFR by using the MTS and MTT assays and using PD 98059 as a negative control for comparison of the anti-inflammatory effect of LFR. Results : The MTS and MTT analysis showed that the cell survival rate was >80% within the LFR concentration range of 10-100 ng/ml and began to decrease to >80% at 1 ${\mu}g/ml$. By RT-PCR analysis, the gene expression of TNF-${\alpha}$, IL-8, TGF-${\beta}$, IL-6, IL-${\beta}$1, and IL-10 levels were down-regulated when monocyte-derived macrophages were treated with concentrations of LFR between 10 ng/mL and 100 ng/mL. Conclusion : We conclude that LFR exerts an anti-inflammatory effect by inhibiting the expression of pro-inflammatory activity. The results suggest a promising way to treat general inflammatory diseases.

UHF RFID 태그 칩용 저전력, 저면적 비동기식 EEPROM 설계

백승면,이재형,송성영,김종희,박문훈,하판봉,김영희,Baek, Seung-Myun,Lee, Jae-Hyung,Song, Sung-Young,Kim, Jong-Hee,Park, Mu-Hun,Ha, Pan-Bong,Kim, Young-Hee 한국정보통신학회 2007 한국정보통신학회논문지 Vol.11 No.12

본 논문에서는 $0.18{\mu}m$의 EEPROM cell을 사용하여 수동형 UHF RFID 태그 칩에 사용되는 저전력, 저면적의 1Kbits 비동기식 EEPROM IP를 설계하였다. 저면적 회로 설계 기술로는 $0.18{\mu}m$ EEPROM 공정을 이용하여 비동기식 EEPROM IP를 설계하므로 command buffer와 address buffer를 제거하였고 separate I/O 방식을 사용하므로 tri-state 데이터 출력 버퍼(data output buffer)를 제거하였다. 그리고 저전압(low voltage)의 VDD에서 EEPROM cell이 필요로 하는 고전압(high voltage)인 VPP와 VPPL 전압을 안정적으로 공급하기 위해 기존의 PN 접합 다이오드 대신 Schottky 다이오드를 사용한 Dickson 전하펌프를 설계하므로 전하펌프의 펌핑단(pumping stage)의 수를 줄여 전하펌프가 차지하는 면적을 줄였다. 저전력 회로 설계 기술로 Dickson 전하 펌프(charge pump)를 이용하여 VPP generator를 만들고 Dickson 전하펌프의 임의의 노드 전압을 이용하여 프로그램과 지우기 모드에서 각각 필요로 하는 VPPL 전압을 선택하도록 하게 해주는 VPPL 전원 스위칭 회로를 제안하여 쓰기전류(write current)를 줄이므로 저전력 EEPROM IP를 구현하였다. $0.18{\mu}m$ 공정을 이용하여 설계된 비동기식 EEPROM용 테스트 칩은 제작 중에 있으며, 비동기식 1Kbits EEPROM의 레이아웃 면적은 $554.8{\times}306.9{\mu}m2$로 동기식 1Kbits EEPROM에 비해 레이아웃면적을 11% 정도 줄였다. In this paper, a low-power and small-area asynchronous 1 kilobit EEPROM for passive UHF RFID tag chips is designed with $0.18{\mu}m$ EEPROM cells. As small area solutions, command and address buffers are removed since we design asynchronous I/O interface and data output buffer is also removed by using separate I/O. To supply stably high voltages VPP and VPPL used in the cell array from low voltage VDD, Dickson charge pump is designed with schottky diodes instead of a PN junction diodes. On that account, we can decrease the number of stages of the charge pump, which can decrease layout area of charge pump. As a low-power solution, we can reduce write current by using the proposed VPPL power switching circuit which selects each needed voltage at either program or write mode. A test chip of asynchronous 1 kilobit EEPROM is fabricated, and its layout area is $554.8{\times}306.9{\mu}m2$., 11% smaller than its synchronous counterpart.

포톤 계수 방식의 $32{\times}32$ 픽셀 어레이를 갖는 디지털 CMOS X-ray 이미지 센서 설계

성관영,김태호,황윤금,전성채,진승오,허영,하판봉,박무훈,김영희,Sung, Kwan-Young,Kim, Tae-Ho,Hwang, Yoon-Geum,Jeon, Sung-Chae,Jin, Seung-Oh,Huh, Young,Ha, Pan-Bong,Park, Mu-Hun,Kim, Young-Hee 한국정보통신학회 2008 한국정보통신학회논문지 Vol.12 No.7

본 논문에서는 $0.18{\mu}m$ triple-well CMOS 공정을 사용하여 포톤계수 방식의 $32{\times}32$ 픽셀 어레이를 갖는 CMOS ray 영상센서를 설계하였다. 설계된 영상센서의 카픽셀은 $100{\times}100\;{\mu}m2$ 면적을 가지고 있고 약 400개의 트랜지스터로 구성되어 있으며, 범프 본딩을 통해 ray 검출기와 CSA(Charge Sensitive Amplifier)의 연결을 위한 $50{\times}50{\mu}m2$의 오픈패드를 가지고 있다. 각각의 싱글픽셀 CSA에서 전압 바이어스 회로를 사용한 folded cascode CMOS OP amp 대신 레이아웃 면적을 줄이기 위하여 self biased folded cascode CMOS OP amp를 이용하였으며, 계수 모드 진입 전후에 CLK에서 발생 할 수 있는 short pulse를 제거하는 15bit LFSR 계수기 (Linear Feedback Shift Register Counter) 클럭 발생회로를 제안하였으며, 읽기 모드에서 CMOS X-ray 영상센서의 최대 전류를 줄이기 위하여 열 어드레스 디코더를 이용하여 한 열씩 읽도록 설계하였다. In this paper, x-ray image sensor of photon counting type having a $32{\times}32$ pixel array is designed with $0.18{\mu}m$ triple-well CMOS process. Each pixel of the designed image sensor has an area of loot $100{\times}100\;{\mu}m2$ and is composed of about 400 transistors. It has an open pad of an area of $50{\times}50{\mu}m2$ of CSA(charge Sensitive Amplifier) with x-ray detector through a bump bonding. To reduce layout size, self-biased folded cascode CMOS OP amp is used instead of folded cascode OP amp with voltage bias circuit at each single-pixel CSA, and 15-bit LFSR(Linear Feedback Shift Register) counter clock generator is proposed to remove short pulse which occurs from the clock before and after it enters the counting mode. And it is designed that sensor data can be read out of the sensor column by column using a column address decoder to reduce the maximum current of the CMOS x-ray image sensor in the readout mode.

DMBA로 유발한 Mouse 피부암에 대한 Astaxanthin이 함유된 난황의 항암효과

이상호,박철우,이영춘,최의성,김무남,하영래,Lee, Sang-Ho,Park, Cheol-U,Lee, Yeong-Chun,Choe, Ui-Seong,Kim, Mu-Nam,Ha, Yeong-Rae 한국환경성돌연변이발암원학회 1998 한국환경성돌연변이·발암원학회지 Vol.18 No.1

Anticarcinogenic activity of astaxanthin-containing egg yolks (designate AEY) was investigated for 7,12-dimethylbenz[a]anthracene (DMBA)-induced two stage mouse epidermal carcinogenesis. Female ICR mouse (6-7 weeks of age) were house in a humidity-and-temperature-controlled facility and subjected to feed and water ad libitum. AEY (10 mg/0.2 ml acetone) was painted on the back of mice 7 days, 3 days and 5 min before DMBA treatment (50 nmole/0.2 ml acetone). One week later after DMBA treatment, 6 ${\mu}g$ tetradecanoyl 12-phorbol 13-O-acetate (TPA) dissolved in 0.2 ml acetone was applied on the mouse twice weekly over a period of 22 weeks. No sample was given to control mice. Control egg yolk (CEY) and astaxanthin-containing oil (designate AO) from Phaffia rhodozyma were used as positive controls. Mouse treated with AEY exhibited 10 tumors per mouse whereas control mouse exhibited 15 tumors per mouse, the fact that 33% reduction of tumor per mouse by AEY treatment. Tumor incidence was also reduced to 15% by AEY treatment when compared to that of control group. Such effects were also seen in CEY and AO treatment groups, but leaser extent. AO gave reduction of food intake and body weights relative to those of AEY and CEY, indicating toxicity of AO. These results suggest that AEY exhibits anticarcinogenic activity for DMBA-induced mouse epidermal carcinogenesis.

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Son, Ji Yeon,Kang, Yoon Jong,Kim, Kyeong Seok,Kim, Tae Hyung,Lim, Sung Kwang,Lim, Hyun Jung,Jeong, Tae Cheon,Choi, Dal Woong,Chung, Kyu Hyuck,Lee, Byung Mu,Kim, Hyung Sik Korean Society of ToxicologyKorea Environmental Mu 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

넓은 전압 범위와 개선된 파워-업 특성을 가지는 밴드갭 기준전압 발생기의 스타트-업 회로

성관영,김종희,김태호,카오투안부,이재형,임규호,박무훈,하판봉,김영희,Sung, Kwang-Young,Kim, Jong-Hee,Kim, Tae-Ho,Vu, Cao Tuan,Lee, Jae-Hyung,Lim, Gyu-Ho,Park, Mu-Hum,Ha, Pan-Bong,Kim, Young-Hee 한국정보통신학회 2007 한국정보통신학회논문지 Vol.11 No.8

본 논문에서는 넓은 동작전압 범위와 저소비 전력 그리고 개선된 파워-업 특성을 가지는 캐스코드 전류 거울형 CMOS 밴드갭 기준전압 발생기의 스타트-업 회로를 제안하였다. 새롭게 제안된 스타트-업 회로는 기존의 스타트-업 회로에 비해 안정적인 파워-업 특성을 가지며 공급전압(VDDA)이 높아지더라도 밴드갭 기준전압 발생기 회로의 동작에 영향을 미치지 않는 것을 모의실험을 통하여 확인하였고 $0.18{\mu}m$ tripple-well CMOS 공정을 이용하여 테스트 칩을 제작하고 측정하였다. 측정 된 기준전압 Vref는 평균값이 738mV이고 $3{\sigma}$는 29.88mV이다. A start-up circuit of the bandgap reference voltage generator of cascode current mirror type with wide operating voltage range and enhanced power-up characteristics is proposed in the paper. It is confirmed by simulation that the newly proposed start-up circuit does not affect the operation of the bandgap reference voltage generatory even though the supply voltage(VDDA) is higher and has more stable power-up characteristic than the conventional start-up circuit. Test chips are designed and fabricated with $0.18{\mu}m$ tripple well CMOS process and their test has been completed. The mean value of measured the reference voltage(Vref) is 738mV and The three sigma value($3{\sigma}$) is 29.88mV.

X-ray 이미지 센서용 싱글 픽셀 포톤 카운터 설계

백승면,김태호,강형근,전성채,진승오,허영,하판봉,박무훈,김영희,Baek, Seung-Myun,Kim, Tae-Ho,Kang, Hyung-Geun,Jeon, Sung-Chae,Jin, Seung-Oh,Huh, Young,Ha, Pan-Bong,Park, Mu-Hun,Kim, Young-Hee 한국정보통신학회 2007 한국정보통신학회논문지 Vol.11 No.2

본 논문에서는 디지털 의료 영상 및 진단 분야 그리고 산업용으로도 활용 가능한 싱글 포톤 계수형 영상센서를 $0.18{\mu}m$ triple-well CMOS(Complementary Metal Oxide Semiconductor) 공정을 사용하여 설계하였다. 설계된 Readout 칩용 싱글 픽셀은 디지털 X-ray 이미지 센서모듈을 간단화 하기 위해 단일 전원전압을 사용하였으며, Preamplifier의 출력 전압인 signal voltage(${\Delta}Vs$)를 크게 하기 위해 Folded Cascode CMOS OP amp를 이용한 Preamplifier를 설계하였으며, 기존의 Readout 칩 외부에서 인가하던 threshold voltage를 Readout 칩 내부에서 생성해 줄 수 있도록 Externally Tunable Threshold Voltage Generator 회로를 새롭게 제안하였다. 그리고, Photo Diode에서 발생하는 Dark Current Noise를 제거하기 위한 Dark Current Compensation 회로를 제안하였으며, 고속 counting이 가능하고, layout 면적이 작은 15bit LFSR(Linear Feedback Shift Resister) Counter를 설계하였다. A single pixel photon counting type image sensor which is applicable for medical diagnosis with digitally obtained image and industrial purpose has been designed with $0.18{\mu}m$ triple-well CMOS process. The designed single pixel for readout chip is able to be operated by single supply voltage to simplify digital X-ray image sensor module and a preamplifier which is consist of folded cascode CMOS operational amplifier has been designed to enlarge signal voltage(${\Delta}Vs$), the output voltage of preamplifier. And an externally tunable threshold voltage generator circuit which generates threshold voltage in the readout chip has been newly proposed against the conventional external threshold voltage supply. In addition, A dark current compensation circuit for reducing dark current noise from photo diode is proposed and 15bit LFSR(Linear Feedback Shift Resister) Counter which is able to have high counting frequency and small layout area is designed.

Characterization of Inkjet-Printed Silver Patterns for Application to Printed Circuit Board (PCB)

Shin, Kwon-Yong,Lee, Minsu,Kang, Heuiseok,Kang, Kyungtae,Hwang, Jun Young,Kim, Jung-Mu,Lee, Sang-Ho The Korean Institute of Electrical Engineers 2013 Journal of Electrical Engineering & Technology Vol.8 No.3

In this paper, we describe the analysis of inkjet-printed silver (Ag) patterns on epoxy-coated substrates according to several reliability evaluation test method guidelines for conventional printed circuit boards (PCB). To prepare patterns for the reliability analysis, various regular test patterns were created by Ag inkjet printing on flame retardant 4 (FR4) and polyimide (PI) substrates coated with epoxy for each test method. We coated the substrates with an epoxy primer layer to control the surface energy during printing of the patterns. The contact angle of the ink to the coated epoxy primer was $69^{\circ}$, and its surface energy was 18.6 $mJ/m^2$. Also, the substrate temperature was set at $70^{\circ}C$. We were able to obtain continuous line patterns by inkjet printing with a droplet spacing of $60{\mu}m$. The reliability evaluation tests included the dielectric withstanding voltage, adhesive strength, thermal shock, pressure cooker, bending, uniformity of line-width and spacing, and high-frequency transmission loss tests.

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Ji Yeon Son,Yoon Jong Kang,Kyeong Seok Kim,Tae Hyung Kim,Sung Kwang Lim,Hyun Jung Lim,Tae Cheon Jeong,Dal Woong Choi,Kyu Hyuck Chung,Byung Mu Lee,Hyung Sik Kim 한국독성학회 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

Original Article : Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

( Ji Yeon Son ),( Yoon Jong Kang ),( Kyeong Seok Kim ),( Tae Hyung Kim ),( Sung Kwang Lim ),( Hyun Jung Lim ),( Tae Cheon Jeong ),( Dal Woong Choi ),( Kyu Hyuck Chung ),( Byung Mu Lee ),( Hyung Sik Ki 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.