The LIM-only transcription factor LMO2 determines tumorigenic and angiogenic traits in glioma stem cells

Kim, S-H,Kim, E-J,Hitomi, M,Oh, S-Y,Jin, X,Jeon, H-M,Beck, S,Jin, X,Kim, J-K,Park, C G,Chang, S-Y,Yin, J,Kim, T,Jeon, Y-j,Song, J,Lim, Y C,Lathia, J D,Nakano, I,Kim, H Macmillan Publishers Limited 2015 CELL DEATH AND DIFFERENTIATION Vol.22 No.9

Glioblastomas (GBMs) maintain their cellular heterogeneity with glioma stem cells (GSCs) producing a variety of tumor cell types. Here we interrogated the oncogenic roles of Lim domain only 2 (LMO2) in GBM and GSCs in mice and human. High expression of LMO2 was found in human patient-derived GSCs compared with the differentiated progeny cells. LMO2 is required for GSC proliferation both in vitro and in vivo, as shRNA-mediated LMO2 silencing attenuated tumor growth derived from human GSCs. Further, LMO2 is sufficient to induce stem cell characteristics (stemness) in mouse premalignant astrocytes, as forced LMO2 expression facilitated in vitro and in vivo growth of astrocytes derived from Ink4a/Arf null mice and acquisition of GSC phenotypes. A subset of mouse and human GSCs converted into vascular endothelial-like tumor cells both in vitro and in vivo, which phenotype was attenuated by LMO2 silencing and promoted by LMO2 overexpression. Mechanistically, the action of LMO2 for induction of glioma stemness is mediated by transcriptional regulation of Jagged1 resulting in activation of the Notch pathway, whereas LMO2 directly occupies the promoter regions of the VE-cadherin gene for a gain of endothelial cellular phenotype. Subsequently, selective ablation of human GSC-derived VE-cadherin-expressing cells attenuated vascular formation in mouse intracranial tumors, thereby significantly prolonging mouse survival. Clinically, LMO2 expression was elevated in GBM tissues and inversely correlated with prognosis of GBM patients. Taken together, our findings describe novel dual roles of LMO2 to induce tumorigenesis and angiogenesis, and provide potential therapeutic targets in GBMs.

자연과에서의 협동적 포트폴리오 작성활동과 효과

오진태,임채성,권해성 부산교육대학교 과학교육연구소 1998 科學敎育硏究 Vol.23 No.-

This study examined the influences of cooperative construction of portfolio about some concepts in life science in the elementary school. These included four concepts of classification of living organism, comparison, ecosystem, and adaptation to environment. Cooperative activities in the construction of portfolio facilitated the acquisition of correct concepts. Especially, highly significant difference in the area of classification of living things appeared between the traditional and cooperative portfolio groups. When we compared the four concepts, the scores of cooperative group about correct concepts were higher than those of individualistic group. It was revealed that the individualistic construction limited the interaction among children, and children and teacher, while the activities of cooperative construction portfolio activated reflective thinking of children. These results suggest the importance of active and various interaction among children-children and children-teacher. There were no significant differences between the individualistic and traditional groups. As the interaction among children-children and children-teacher activated, it became general trends that children corrected, modified, or complemented their portfolios and became more enthusiastic in such activities. Whole results implied that cooperative and interactive activities have positive influences in the acquisition of correct science concepts.

자연 동굴 토양 방선균의 속 다양성 분포

임채영,권오성,김판경,박동진,이동희,김창진 한국산업미생물학회 1996 한국미생물·생명공학회지 Vol.24 No.5

국내에 소재 하는 자연 동굴 중 충청북도의 고수굴, 천둥굴, 노동굴, 강원도의 고씨굴, 경상북도의 성류굴, 제주도의 협재굴, 쌍용굴, 만장굴 등 8개 동굴의 내부에 존재하는 토양 방선균 분포를 조사하였다. 그 결과 전체적인 속 수준의 분포로서는 Streptomyces가 52.5%, Micromonospora가 16.3%, Nocardioform 22.8% 정도 분포하고 있었으며 Kineosporia, Actinomadura, Nocardiopsis, Streptosporangium의 순서로 방선균이 0.3~1.4% 정도 분포하고 있었다. 지금까지 보고된 일반적인 방선균 분포와 비교해 볼 때 특히 Streptomyces의 분포 비율이 낮고 상대적으로 Micromonospora, Nocardioform이 많이 분포하고 있었다. 한편 노동굴 지역을 대상으로 동굴 내부와 외부로 나누어 방선균 분포를 비교해 보았다. 그 결과 동굴의 내부에는 Streptomyces가 52.0%, Micromonospora가 16.8%, Nocardia가 32.0% 정도 분포하였고 외부에서는 Streptomyces가 75.8%, Micromonospora가 10.7%, Nocardia가 7.1% 정도 분포하였다. 따라서 동일 지역 내에서도 동굴의 내부와 외부에 따라서 큰 차이를 나타내고 있었다. 그리고 분리된 방선균 중에서 Streptomyces속 균주의 형태적인 특징을 조사한 결과, 기균사의 색에 있어서는 회색 계열 45.7%, 적색 계열 7.8%, 황색 계열 2.3%, 녹색 계열 13.2%, 백색 계열 27.1%, 불량 3.9%의 분포를 나타내었다. 일반적으로 Streptomyces속 방선균에서 잘 분리되는 회색과 적색 계열의 분리 비율은 크게 낮아 졌으나 희소한 녹색과 백색 계열은 분리 비율이 크게 증가하였다. 그리고 성류굴에서는 황색, 녹색, 백색, 계열등 다양한 계열의 Streptomyces속 방선균이 많이 분리되었다. 포자 연쇄의 형태에 있어서는 retinaculiaperti 형태가 35.7%, spirales 형태가 12.4%, rectiflexibiles 형태가 51.9%로 나타났다. Total 276 soil actinomycete strains were isolated from 46 soil samples collected at domestic natural caves; the Kosu, Chundong, and Nodong caves at Chungbook province, the Kossi cave at Kangwon province, the Sungruye cave at Kyungbook province, the Hyupjae, Ssangyoug, and Manjang caves at Cheju province. All of these isolates were identified to the genus level based n morphological and physiological characteristics. As the result, 52.5% of those isolates were Streptomyces, 16.3% were Micromonospora, 22.8% were Nocardioform group, 1.1% were Actinomadura, 0.3% were Nocardiopsis, 0.3% were Streptosporangium, 0.3% were Nocardioides, 1.4% were Kineosporia, 4.7% wee the others. Streptomycete strains were the most abundant, but were relatively less comparing to general distribution pattern. Nocardioform and Micromonospora strains were quite abundant, and other rare actinomycete groups were somewhat abundant comparing to general distribution pattern previously reported. Especially Nocardioform strains were highly abundant at almost of the natural caves.

수분 조건에 따른 수종 식물의 기공 분포 변화

오진태,임채성 부산교육대학교 초등교육연구소 1995 초등교육연구 Vol.6 No.-

This study examined the changes of stomatal distribution(density, frequency) in a few plants with the variation of changes in soil-water. Commelina communis L., which is one of C_3 plants, have anomocytic type of stomata. Digitaria sanguianalis Scop. which belongs to C_4 plants, and Portulaca oleracea L. which is CAM plants, have paracytic, and anisocytic types of stomata, respectively. In C. communis L., the stomatal size of upper side of leaves was greater than that of lower parts. In both of D. sanguianalis Scop. and P. oleracea L., this trend was opposite. As the moisture in soils decreased, the stomatal size was decreased. The stomatal densities in these three species were greater in lower sides than those of upper sides. Finally, the stomatal densities of these plants showed trends of more stomata per unit area as the moisture of soil decrease. These results suggest that the plants adopt the efficient water-use strategies.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

교육대학원에 있어서 초등 과학교육 영역의 중요성과 그 운영방안

오진태,옥치율,한영욱,이형철,김용권,임채성 釜山敎育大學校 1994 부산교육대학 논문집 Vol.30 No.1

The future society is expected to be rapidly change in all areas, that is, the length of change time is becoming much smaller than that of generation time. It is very important to prepare the citizens who possess scientific literacy which actively cope with the rapid changes and specialization of society. The sources of rapid changes may be due to the developments in science and technology. Therefore, the importance of science education will be increasingly high. Scientific literacy should be improved basically and systematically from the early stages of formal education. Teachers who are responsible to educate children should be able to research in the areas of science education as well as science itself, and to have capacity to transmit them to the classroom. To do this, the graduate course and the science education within the system beyond the level of college education are essential parts of teacher education. In the graduate course, it is important that science itself and pedagogy of science should be balanced.

다발성 외상환자에서 필수적 단순 방사선검사의 의의

임경수,황성오,임종천,이부수,이강현,이진웅 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

In the guidelines of Advanced Trauma Life Support(ATLS), essential plane roentgenograms (chest AP vIew, cervical spine lateral view and pelvis AP view) are recommanded when primary survey and resuscitation phase are finished in care of multiple injured patients. A prospective study was carried on 376 multiple trauma patients who admitted to Wonju Christian Hospital from July, 1995 to October. We analyzed clinical significance of essential X-ray in multiple trauma patients, and classified the patients by their status of mentality. The one is alert group when their Glasgow Coma Scale(GCS) is over 13 point, the other is disoriented mentality group when their GCS are below 12 point. The alert group was redivided by presence of clnical symptoms and/or signs, and clinical significances of essential X-ray were analyzed. Among the 376 patients, 330 patients are alert group that their GCS score is over 13 point and 46 patients are disoriented group who's GCS were below 12 point. The detection rate of abnormality in essential X-ray is higher in disoriented group than alert group(cervical-spine lateral view : 13.5% vs 3.0% cheat AP view : 0.4%, pelvis AP view : 0%). This study suggests that their are no needs of taking essential X-ray in patients who is alert in mental state and have no clinical symptoms and/or signs.

혼수상태의 외상환자에서 초음파를 이용한 흉부 및 복부손상의 진단

임경수,이강현,이진웅,이부수,황성오,유수영,강성준 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

The need for rapid diagnosis and treatment of life-threatening thoracic and intra-abdominal injury result in controversy over the appropriate triage of unconscious blunt trauma patients. To aid in early decisions for these patients, a prospective analysis of 98 patients with glasgow coma scale(GCS) scores≤8 was undertaken. Although intra-thoracic and intra-abdominal injuries were frequently identified based on systolic blood ressure, the use of clinical signs alone resulted in more missed injuries than did using the emergency ultrasonography. In normotensive patients(n=34), intra-thoracic injuries was identified in one patient(2.9%), and intra-abdominal injuries were in 7 patients(20.6%). In shock(systolic blood pressure<90mmHg) Pa-tients(n=64), thoracic injuries and intra-abdominal injuries were diagnosed in 8(12.5%) and 27 patients(42.3%), thoracic and abdominal combined injuries were identified in 11 patients(17.2%). The sensitivity, specificity, and accuracy of emergency ultrasonography were 96.3%, 91.7%, 94.9%. This study suggests that all unconscious trauma patients undergo immediate emergency ultrasonography to prevent missing life-threatening injuries.

Terfenadine과 Ketoconazole 병용투여에 의해 유발된 Torsade de Pointes1예

오석규,국향,임수빈,정진원,박양규,박옥규 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.3

외상후 혈뇨환자에서 응급 초음파검사의 의의

임경수,이강현,황성오,이진웅 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

The Ultrasonography is rapid, mobile, and noninvasive diagnostic tool in emergency center. As a result, we estimate it`s value as an advocated primary diagnostic modality in the immediate evaluation of patients with multiple trauma. We investigated 60 patients with traumatic hematuria(microscopic hematuria or gross hematuria) who visited our emergency department from 1995, July to 1996, March. To evaluate the injury of urinary systems, emergency physician examined patients with hematuria by ultrasonography, and comapared the results with those of excretory urography and computed tomography. We demonstrated 21 positive findings for abnormal fluid collection and renal parenchymal injury and bladder rupture on ultrasonography. All bladder injuries were diagnosised by ultrasonography, and all major renal injury(grade Ⅲ,Ⅳ,Ⅴ) were also diagnosised by that. The accuracy of emergency ultrasound examination in traumatic hematuria was 97.0%, and sensitivity was 95.0% with specificity of 97.4%. The ultrasonography was an accurate diagnostic adjunct in detecting renal injury and bladder injury in traumatic hematuria patients. Ultrasonography provides an excellent noninvasive diagnostic tool for the initial screening study of patients with traumatic hematuria.