n-CdS_(0.46)Se_(0.54)/p-Cu_92-x)S_(0.46)Se_(0.54) 이종접합 태양전지의 제작과 그 특성에 관한 연구

유상하,최승평,이상열,홍광준,서상석,김혜숙,전승룡,윤은희,문종대,신영진,정태수,신현길,김택성,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

승화방법에 의해 CdS_0.46Se_0.54 단결정을 성장하여 결정구조를 조사하고, Van der Pauw 방법으로 Hall effect를 측정하여 carrier density의 온도 의존성과 mobility의 온도 의존성을 조사하였다. 성장된 CdS_0.46Se_0.54 단결정을 치환반응하여 n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 이종접합 태양전지를 제작하였다. Spectral response, 전류-전압특성 및 전력변환 효율을 조사하여 그 결과로부터 개방전압은 0.48V, 단락 전류 밀도는 21mA/㎠, fill factor와 전력변환효율은 각각 0.75와 9.5%를 얻었다. CdS_0.46Se_0.54 single crystal was grown by a sublimation method. The crystal structure and the temperature dependence of carrier density and mobility of CdS_0.46Se_0.54 single crystal were studied. Heterojunction solar cells on n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 were fabricated by the substitution reaction. The spectral response, the J-U characteristics and the conversion efficiency of the n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells were studied. The open-cricuit voltage, short-circuit density, fill factor and conversion efficiency of n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells under 80mW/㎠ illumination were found to be 0.48V, 21mA/㎠, 0.75 and 9.5%, respectively.

소배양법으로 배양한 생쥐췌장 베타세포의 형태학적 검정

유광성,전현우,박주홍,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

생쥐췌장 베타세포를 보다 순수하게 배양하기 위해 변형 고안한 소배양법을 검정하였다. 이 소배양법에 있어서는 96well plate에 islet cell을 이식하고 decantation한 다음 thimerosal에 노출시켜 많은 viable cell을 얻을 수 있었고, 생후 7일째의 생쥐의 췌장베타세포의 생존율이 가장 높았다. 또 이 소배양법으로 배양한 생쥐췌장 베타세포들은 위상차현미경, FITC-labelled anti-insulin antibody 염색 및 전자현미경으로 검정한 결과 형태학적으로 정상이었다. 기계적 선택방법으로서 생쥐췌장 베타세포의 decantation 최적시간은 세포를 이식한 후 12∼15시간 이었으며, 화학적 선택방법으로 0.6∼0.8㎍/㎖의 thimerosal에 노출시켜 fibroblastoid cell로 부터 생쥐췌장 베타세포의 분리를 증가시킬 수 있었으며, 두가지 방법을 동시에 응용하여 더 좋은 효율을 얻을 수 있었다. 또 이 소배양법에 있어서 cell duplication은 배양후 36시간후에 2배, 72시간후에 3배가 되었으며, 평균 generation시간은 46.8±7.0시간 이었다. 이들 결과로 미루어 보아 이소배양법은 생쥐췌장 베타세포 배양의 유용한 방법으로서, 췌장 베타세포에 연관된 각종 연구에 이용할 수 있을것으로 사료된다.

부산·경남지역의 토양오염물질간의 상관도에 관한 연구

주유연,조상원,오광중,손병현 부산대학교 환경문제연구소 1994 環境硏究報 Vol.12 No.-

It appeared over all regions expect common life area that the pollutants level of soil heavy metals in Pusan and kyoung-Nam Province, was higher than the standard on in agriculture area. Especially, Atmosphere and waste area appeared much high and it necessitates monitoring these areas continually. Where we analyzed pollutants level of heavy metals in paddy soils in correspondence with Pusan, Ulsan, and Yangsan region, the order of Pb and Cd concentration were Yangsan 〉 Pusan 〉 Ulsan, the order of Cu and Zn were Yangsan 〉 Ulsan 〉 Pusan, the order of Hg and As were Ulsan 〉 Yangsan 〉 Pusan respectively. It appeared the whole concentration of heavy metals expect Hg and As were the highest ones in Yangsan. So, it was thought that special management should be needed. The analysis of correlation between heavy metals in soil showed that the correlation level between Cd and Pb, Cu and Pb and Zn, Hg and As was high in agricultural area and the correlation level between Zn and Pb, Cd and Hg, Cd and Cu was high but the one between Cd and As was low in atmosphere area. And the correlation between Pb and Cd, Hg and Zn, Hg and Cu was high but the one between Hg and Cu was low in waste area and the correlation level between As and Pb, As and Zn was slightly high but the one between Cd and Cu is low in water area.

Trapezoidal 구동형 브러시리스 직류 전동기의 최적 정류

禹廣俊,李現彰,庾正鳳 단국대학교 1995 論文集 Vol.29 No.-

In this paper, we analyze the optimal commutation of the trapezoidal driving brushless dc motor which has the trapezoidal flux in the airgap. On the basis of this analysis, we design the hard-wired digital-based optimal commutation control system of the trapezoidal driving brushless dc motor. The optimal commutation circuit consists of a commutation angle ROM, a commutation pattern ROM, and presettable counter. Preposed scheme is simple and quite adequate to the high speed operation. As the analysis shows, we confirm that experimental results show the improved driving characteristics, e. g., the torque is increased over all of the operating range, and the operating range is widely enlarged.

DSR을 기반으로 라우팅 테이블을 적용한 개선된 Ad hoc라우팅에 관한 연구

김승수,유충렬,이광배,김현욱 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

In this paper, an improved source routing protocol is designed for mobile ad hoc network with unidirectional link. In the Mobile ad hoc network, nodes communicates with each other by usingmulti-hop method without any base station. In order for a source mobile node to send its data to a destination node through other intermediate nodes, mobile ad hoc routing protocol is required. DSR and AODV are the representative routing protocols for Mobile ad hoc network. However, unfortunately, current DSR and AODV routing protocol versions support mobile ad hoc network only with bidirectional links. We proposed and simulated a source routing protocol supporting mobile ad hoc network with unidirectional links which was based on DSR and AODV routing protocol. The result of simulation show average route setting time of 0.004~0.007 second and average date receiving rate of 97~97%.

Escherichia coli에서 발현된 재조합 인간 상피세포 증식인자의 정제 및 특성

박세철,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

E. coli BL21(DE3, pYHB101)을 이용한 rhEGF의 최적 생산조건은 25℃, 48시간 배양에서 변형된 MBL 배지에 glucose를 10g /l 첨가한 배지로 배양한 경우이었고 유도물질로 1mM IPTG를 사용하여 2시간 배양 후에 유도 배양하였을 때 최대 rhEGF가 68.7 mg/l가 발현되었다. 배양액으로부터 Amberlite XAD-7, ultrafiltration, DEAE Sepharose column chromatography 정제 과정을 거쳐 rhEGF가 정제되었으며 이때 정제도는 267배이었으며 66.6%의 회수율을 보였다. 정제된 rhEGF는 HPLC 분석 결과 2개의 분획으로 나누어졌으며 N말단 아미노산 서열 분석결과 Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His로 나타났다. 또한 5'-bromo-2'-deoxy-uridine (BrdU)의 삽입에 의한 DNA 생성능을 조사하였다. 상기의 결과로 E. coli BL21(DE3, pYHB101)에 의하여 생산된 rhEGF는 nhEGF와 동일한 것으로 추정되었다. Recombinant human epidermal growth factor (rhEGF) was produced strain was cultured at 25℃ for 48 hours in the modified MBS medium containing 10 g/l glucose with 1 mM IPTG induction at 2 hours after inoculation. The rhEGF was purified upto 267 folds by Amberlite XAD-7 chromatography, ultrafiltration, and DEAE Sepharose fast fow ion exchange chromatography with an overall yield of 66.6%. The purfied rhEGF was further separated into two fractions by HPLC. The N-terminal amino acid sequence of the second fraction was Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His. The effect of rhEGF on the DNA synthesis was examined using in vitro biological assay based on the incorporation of 5'-bromo-2'-deoxy-uridine (BrdU). The purified rhEGF shows no difference with natural human epidermal growth factor (nhEGF) in N-terminal amino acids residues and biological activity. From the results, we concluded that rhEGF produced from E. coli harboring the plasmid pYHB101 was apparently the same as nhEGF.

임신부 뇨로부터 정제된 인간 상피세포 증식 인자 유사체의 in vitro bioassay 및 특성

박세철,전재현,남정현,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

벤조산흡착, 음이온 교환수지, 단일클론항체을 이용한 immunoaffinity chromatography를 통하여 임신부 뇨로부터 천연의 hEGF를 정제하였다. 정제된 hEGF는 μ Bonda C_18 column을 사용한 HPLC 분석을 통하여 4개의 fraction으로 분리가 가능하였으며 western blot과 double immunodiffusion 실험 결과, 각각의 fraction이 hEGF의 특성을 가진 유사체인 것을 알 수 있었다. 또한 hEGF 표준 물질과의 spiking 및 아미노산 분석 등을 통하여 두 번째 fraction이 nhEGF와 동일한 것으로 확인하였다. nhEGF 및 그 유사체의 생물학적 활성 비교를 위하여 NIH 3T3 세포주에서 5'-Brdu incorporation 측정을 위한 labelling 시간, 혈청 농도의 최적 조건을 결정하였다. NIH 3T3 세포주의 DNA 합성능은 0.2% FCS가 포함된 저혈청 배지에서 hEGF가 0.1~10 ng/ml 농도로 첨가하였을 때 증가하는 경향을 나타냈다. HPLC를 통하여 분리된 두번째 hEGF 유사체가 다른 유사체보다도 생물학적인 활성이 우수하였으며, rhEGF 표준물질과의 spiking 및 아미노산 서열 분석등을 통하여 nhEGF로 밝혀졌다. 임신부의 뇨의 hEGF 유사체 함량중 natural hEGF는 46%이었다. Natural human epidermal growth facto (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C_18 HPLC system. Following characterization by Western blot and double immunodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporation of 5-bromo-2'-deoxy-uridine (BrdU), a non-radioactive alternative for [^3H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1~10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others.

MANET 라우팅 프로토콜 QoS 지원서비스의 성능평가에 관한 연구

鄭燦赫,金鉉郁,李光培,姜景仁,朴京培,劉忠烈,吳世德,裵振勝,趙榮泰 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In this paper, we evaluated two main QoS supporting routing protocol on wireless ad hoc network. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS support service between source and destination becoming a very important issue. Of all routing protocols, DSR and AODV are very important routing protocol in MANET. So we simulated DSR and AODV QoS Routing Protocol Through simulation evaluation tool NS(Network Simulation) based on various environments.

PelB Signal Sequence로 유도된 제조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

박세철,남정현,김정근,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

전사개시 단계중에 삽입되는 N말단의 methionine이 제거되고 pelB signal sequence를 삽입하여 인체 epidermal growth factor (hEGF)가 직접 분비발현 되도록 고안된 T7과 tac promoter에 의하여 각각 발현되는 pYHB101과 pYHB2를 제조하였다. 또한 T7 promoter에 의하여 발현되지만 signal sequence를 삽입하지 않은 pYHB1도 제조하여 각각 제조된 재조합 plasmid들의 발현 및 분비효율을 비교하였다. pelB signal sequence hEGF 유전자를 갖도록 제조된 pYHB101을 포함하고 있는 균주가 pYHB1보다 균체 성장속도는 낮았지만 pYHB101을 1 mM IPTG로 5시간 induction 하였을 때 13 mg/l의 rhEGF가 분비발현되어 T7 promoter의 발현 체계를 갖는 경우가 tac promoter보다 높은 발현 효율을 보였다. 이때 pYHB101에서 발현되는 rhEGF의 발현량은 준최적 조건에서 14 mg/l이었다. We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

GMAHN에서의 네트워크 성능 향상에 관한 연구

金在浩,李光培,金玄郁,劉忠烈,河載承,裵振勝 明知大學校 産業技術硏究所 2008 産業技術硏究所論文集 Vol.27 No.-

Recently, Wire-rireless combination study is being studied very actively with the advent of Ubiquitous environment, using wireless device such as PDA, Cellular Phone loading multi-hop communication that connects wired internet network very easily and controls the system and shares the information in anyplace. The past algorithm, wired and wireless combination that connect between Ad Hoc Network and wired network is being focused only on the study of heterogeneous network interface. The proposed algorithm established independent multi-path between source node and destination node to prevent data loss when errors happen in route. The proposed algorithm established independent multi-path between source node and destination node to prevent data loss, and trasmitted data packet to destination node very quickly and promoted network reliabiliy when errors happen in route.