養液濃度와 培地種類가 Black Olympia 포도나무의 生育과 果實品質에 미치는 影響

김희곤,김광수,김월수,임경호,최경주,김상철,김병삼 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

본 硏究는 養液栽培時 養液濃度와 培地種類가 대립계 포도품종인 Black Olympia의 生育과 果實品質에 미치는 영향을 구명하고자 하였다. 新梢長은 0.5배에서 1.5배의 양액농도에서 농도가 높을수록 길었고, 배지종류별로는 0.5배 농도에서는 밭흙과 퇴비를 1:1로 혼합한 배지에서 신초장이 길었으나 1.5배 농도에서는 오히려 짧게 나타났다. 節間長은 저농도의 양액농도에서는 밭흙과 퇴비를 혼합한 배지가 길었으나 1.5배의 고농도에서는 차이를 보이지 않았다. 각 배지에서 濃度가 높을수록 간경이 크게 나타났으며 배지종류간에는 펄라이트와 버미큘라이트 혼합배지가 밭흙과 퇴비를 혼합한 배지보다 컸으나 유의차가 없었다. 果房重은 각 배지에서 1.0배의 양액농도가 가장 무거웠으며 배지 종류간에는 밭흙과 퇴비를 혼합한 배지가 더 무거웠다. 糖度는 각 배지의 1.0배 양액농도가 높았으며 배지종류간에는 차이를 보이지 않았다. 果皮의 안토시아닌 함량은 배지별로 밭흙과 퇴비를 혼합한 배지에서 높았으며 양액농도별로는 1.0배에서 높게 나타났다. 收量은 펄라이트와 버미큘라이트를 혼합한 배지에서 양액농도가 높을수록 많았으나 밭흙과 퇴비 혼합배지에서는 1.0배의 농도에서 높게 나타났다. This study was carried out to investigate the effects of ionic strength and substrates on growth and fruit quality, and to establish fruit tree nutrient solution culture. The higher ionic strength showed the longer shoot length of grape. Among the substrates, upland soil+compost showed longer shoot length than perlite+vermiculite in 0.5 strength but shorter in 1.5 strength of Hoagland solution. Internode length was longer in upland soil+compost than in perlite+vermiculite in 0.5 strength, but there were no significance in 1.0 and 1.5 strength of Hoagland solution. Higher ionic strength and/or perlite+vermiculite of substrates showed tricker in trunk diameter than lower ionic strength and/or upland soil+compost. Cluster weight was heavier in 1.0 ionic strength than the others. Cluster weight in upland soil+compost was heavier than that of perlite+vermiculite. Soluble solids content of berry was the highest as much as 17.3。 Bx on 1.0 ionic strength of three strength, but there are no significances in substrates. Anthocyanin content of grape skin was higher in upland soil+compost than perlite+vermiculite, as well as the highest in 1.0 strength among three strengths. The higher ionic strength showed the more berry set. Each substrate showed uniformal berry set and growth. The best result in berry yields was obtained in 1.0 ionic strength with substrate of upland soil+compost in comparison to other treatments.

우라늄오염에 의한 신부전증에 미치는 제염제의 방호효과

김태환,정인용,김성호,김경중,방효창,류성렬,진수일 대한방사선 방어학회 1990 방사선방어학회지 Vol.15 No.2

원자력산업의 시설증대로 우라늄 오염의 가능성이 증가되고 있음에도 불구하고 종사자 및 국민에대한 안전대책 및 의료적 처치에 관한 연구가 매우 미흡한 실정에 있어 이에 대한 응급처치방안을 수립코자 우라늄을 투여한 후 제염제를 투여하여 관찰하였던 바 다음과 같은 결론을 얻었다. 1. 우라늄오염에 의한 체중변화에 미치는 제염제의 영향에 있어서 sodium bicarbonate와 생리적 식염수를 병행투여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol을 투여한 군에서는 우라늄단독투여군에 비해 체중이 현저하게 증가하였다. (P<0.05). 2. 모든 실험군은 우라늄오염에 의한 음수량과 배뇨량의 변화를 유의성 있게 호전시켰으며(P<0.05), 특히 sodium bicarbonate와 생리적 식염수를 병행투여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol을 투여한 군이 가장 높은 증가경향을 나타냈다(P<0.05). 3. 우라늄오염에 의한 BUN농도 변화에 미치는 제염제의 효과는 sodium bicarbonate와 생리적 식염수를 병행투여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군이 공히 우라늄단독투여군보다 BUN농도가 매우 감소되었다(P<0.01). 4. 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군은 우라늄오염에 의한 serum creatinine의 농도증가를 유의하게 감소시켰으나 (P<0.01), sodium bicarbonate와 생리적 식염수를 병행투여한 군은 다소 감소하는 경향으로 나타났다. 5. 우라늄오염에 의한 urine creatinine농도 변화에 미치는 제염제의 효과에 있어서 sodium bicarbonate와 생리적 식염수를 병행토여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군에서는 우라늄단독투여군에 비해 creatinine의 배설이 상당히 증가하였다(P<0.05). 6. 우라늄오염에 의한 신장의 소견에 있어 우라늄단독투여군은 근위곡세뇨관상피의 공포화 및 종창, microvilli와 brush border의 손실, 세뇨관 상피의 괴사가 관찰되었으며, 간장의 충혈, 중심성 괴사 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군에서는 우라늄단독투여군에 비해 높은 방호효과가 관찰되었으나 다른 실험군에서는 큰 효과가 없는 것으로 나타났다. 결론적으로 우라늄의 체내오염시에는 sodium bicarbonate와 생리적 식염수를 가능한 빨리 병행투여하거나 dithiothreitol을 체내오염후 30분이 지나서 투여하는 방법이 우라늄오염에 대한 제염에 매우 유효할 것으로 생각되며, 특히 우라늄에 의한 인체장해를 유의하게 경감시켜줄 것으로 사료되었다. Appreciable radiation exposures certainly were occurred m the reactor burn-up, the nuelear fall-out and the surroundings of nuclear installations with radioactive effluents. Therefore ,radioactive nuclides is not only potentially hazardous to workers of nuclear power plants and related industrials, but also the wokers who handle radioactive nuclides in biochemical research and nuclear medicine diagnostics. And in the case of occurring the nuclear accidents, the early medical treatment of radiation injury should be necessary but little is established'medical procedures to decontaminate the victims of internal contamination of radioactive nuclides in korea. Accordingly, to achieve the basic data for protective roles and medical treatment of radiation injury, the present studies were can-id out to evaluate the decontamination of uranium by the chemical drugs. The results observed were summarized as follows : 1. The combined treatmet group of sodium bicarbonate and saline with uranyl nitrate injection simultaneously and the dithiothreitol group that was administered 30 minutes after uranyl nitrate injection were increased significantly in the change of body weight than uranyl nitrate-only group(P<0.005). 2. All the experimental groups were increased the fluid intake and urine volume on the uranyl nitrate-induced acute renal failure, but the combined treatment group of sodium bicarbonate and saline with uranyl nitrate injection simultaneously and the dithiothreitol group that was administered 30 minutes after uranyl nitrate injection have the higher increment of fluid intake and urine volume(P<0.05). 3. When sodium bicarbonate and saline was treated with uranyl nitrate injection simultaneously, and dithiothreitol was administered 30 minutes after uranyl nitrate injection, there was significantly reduced in BUN concentration (P<0.0l). 4. When dithiothreitol was administered 30 minutes after uranyl nitrate injection, there was reduced more significantly on the increment of serum creatinine concentration than that observed in uranyl nitrate-only group(P<O.Ol). but when the combined treatment of sodium bicarbonate and saline with uranyl nitrate simultaneously, there was still, albeit much less marked, decrease in serum creatinine concentration. 5. The sodium bicarbonate and saline was treated with uranyl nitrate simultaneously and dithiothreitol was administered at 30 minutes after uranyl nitrate were excreted markedly higher urine creatinine concentration than the uranyl nitrate-only group. 6. Uranyl nitrate has been used in experimental animals to produce hydropic degeneration and swelling of proximal tubules, disappearance of microvilli and brush border or necrosis in the kidney and centrilobular necrosis, congestion, and telangiectasia of the liver. When the sodium bicarbonate and saline was treated with uranyl nitrate simultaneously, and dithiothreitol was administered 30 minutes after uranyl nitrate, there was more marked the protective effect than uranyl nitrate-only group. Finally, if the sodium bicarbonate and saline may administered as quickly as possible each time that some risk for internal contamination with uranium, and dithiothreitol is administered 30 minutes after uranium contamination, there ameliorates the course of uranyl nitrate-induced acute renal failure,and this effect is assocciated with prevention of uranium (heavy metal) induced alterations in BUN. serum creatinine. urine creatinine. fluid intake, urine volume and body weight.

경부 관통상으로 인한 사지 마비 1례

박 진,이경운,김춘호,김성중,조남수,안태훈 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

A case of quadriplegia resulted from cervical penetrating wound was admitted to chosun university hospital intensive care unit, and undergone steroid therapy and antibiotics therapy. On the 6th hospital day, the septic complication was developed and it proved to be bacterial meningitis by CSF examination. Broad spectrum antibiotics was administered immediately after and the diagnosis and the patient was monitored carefully. The bacterial meningitis was improved and no other serious complication was developed. The patient was discharged with permanent neurologic deficit, quadriplegia.

Direct Synthesis of Polymer Nanocapsules with a Noncovalently Tailorable Surface

Kim, Dongwoo,Kim, Eunju,Kim, Jeeyeon,Park, Kyeng ,Min,Baek, Kangkyun,Jung, Minseon,Ko, Young ,Ho,Sung, Wokyung,Kim, Hyung ,Seok,Suh, Ju ,Hyung,Park, Chan ,Gyung,Na, Oh&#x WILEY-VCH Verlag 2007 Angewandte Chemie Vol.46 No.19

<B>Graphic Abstract</B> <P>Hollow out your pumpkin: The direct synthesis of approximately 100-nm-diameter polymer nanocapsules was carried out in the absence of preorganized structures or templates. The method appears to be applicable to any monomer that has a flat core and multiple polymerizable groups at the periphery (see picture). The surface properties of the polymer shell, which comprises cucurbituril, can be easily tailored through host–guest chemistry. <img src='wiley_img/14337851-2007-46-19-ANIE200604526-content.gif' alt='wiley_img/14337851-2007-46-19-ANIE200604526-content'> </P>

Functionalized cucurbiturils and their applications

Kim, Kimoon,Selvapalam, Narayanan,Ko, Young Ho,Park, Kyeng Min,Kim, Dongwoo,Kim, Jeeyeon Royal Society of Chemistry 2007 Chemical Society reviews Vol.36 No.2

<P>Cucurbit[<I>n</I>]uril (CB[<I>n</I>], <I>n</I> = 5–10), a new family of molecular hosts comprising <I>n</I> glycoluril units, have gained much attention in the new millennium for their exceptional molecular recognition ability. The CB homologues have brought dynamism to CB chemistry, as witnessed by the heightened interest in the field for the last several years. Compared to the chemistry of cyclodextrins and calixarenes, however, that of CB[<I>n</I>] has developed slowly until recently, which may be attributed mainly to their poor solubility in common solvents, and inability to functionalize these molecules. The direct functionalization method of CB[<I>n</I>] propelled CB chemistry to a new height as this new method not only solved the solubility problem but also opened up the gateway to the generation of tailor-made CB[<I>n</I>] derivatives. The functionalization of CB[<I>n</I>] led us to investigate numerous applications including artificial ion channels, vesicles, stationary phases in chromatography, ISEs, polymers, nanomaterials, and many others. This tutorial review describes the recent advances and challenges in the functionalization of CBs along with the applications of functionalized CBs.</P> <P>Graphic Abstract</P><P>The recent advances and challenges in the functionalization of cucurbiturils (CBs) along with the applications of functionalized CBs are described. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b603088m'> </P>

만성 기도질환에서 흰쥐 뮤신에 대한 단 세포군 항체 (RTO3)를 이용한 뮤신 측정에 관한 연구

김도진 ( Do Jin Kim ),김기업 ( Ki Up Kim ),남궁은경 ( Eun Kyeng Namgung ),어수택 ( Soo Taek Uh ),김용훈 ( Young Hoon Kim ),신찬영 ( Chan Young Shin ),고광호 ( Kwang Ho Ko ),박춘식 ( Choon Sik Park ) 대한결핵 및 호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.47 No.6

초소형 무인 방제보트를 위한 원격 조종장치 개발

김헌희(Heon-Hui Kim),하윤수(Yun-Su Ha),김환성(Hwan-Sung Kim),민경식(Kyeng-Sik Min),문제은(Je-Eun Moon),최문성(Moon-Sung Choi),김대현(Dae-Hyun Kim),강군호(Goon-Ho Kang),고운용(Woon-Yong Koh) 한국마린엔지니어링학회 2003 한국마린엔지니어링학회 학술대회 논문집 Vol.2003 No.-

It is very important to monitor the spread of spilled oil for effective response planning. However, the use of traditional response ship or other response equipment for this purpose under wild sea condition such as high wave, fast tide and wind is limited because of physical fatigue and stress of crews. Therefore, the development of an unmanned pollution response vehicle which can monitor the spread of spilled oil under wild sea condition is needed. In this paper, we report the construction of remote control system for super mini-size unmanned pollution response vehicles which consists of RF controller and a GUI-based control consol.<br/> <br/>

Direct Profiling the Post-Translational Modification Codes of a Single Protein Immobilized on a Surface Using Cu-free Click Chemistry

Kim, Kyung Lock,Park, Kyeng Min,Murray, James,Kim, Kimoon,Ryu, Sung Ho American Chemical Society 2018 ACS central science Vol.4 No.5

<▼1><P/><P>Combinatorial post-translational modifications (PTMs), which can serve as dynamic “molecular barcodes”, have been proposed to regulate distinct protein functions. However, studies of combinatorial PTMs on single protein molecules have been hindered by a lack of suitable analytical methods. Here, we describe erasable single-molecule blotting (eSiMBlot) for combinatorial PTM profiling. This assay is performed in a highly multiplexed manner and leverages the benefits of covalent protein immobilization, cyclic probing with different antibodies, and single molecule fluorescence imaging. Especially, facile and efficient covalent immobilization on a surface using Cu-free click chemistry permits multiple rounds (>10) of antibody erasing/reprobing without loss of antigenicity. Moreover, cumulative detection of coregistered multiple data sets for immobilized single-epitope molecules, such as HA peptide, can be used to increase the antibody detection rate. Finally, eSiMBlot enables direct visualization and quantitative profiling of combinatorial PTM codes at the single-molecule level, as we demonstrate by revealing the novel phospho-codes of ligand-induced epidermal growth factor receptor. Thus, eSiMBlot provides an unprecedentedly simple, rapid, and versatile platform for analyzing the vast number of combinatorial PTMs in biological pathways.</P></▼1><▼2><P>An <U>e</U>rasable <U>si</U>ngle <U>m</U>olecule <U>blot</U> (eSiMBlot) assay provides an unprecedentedly simple and versatile platform for analyzing the combinatorial post-translational modifications in biological pathways.</P></▼2>

Characteristics of Gas Generation by Injecting Organic Wastewater into Lysimeters Filled with Waste

( Kyeng-ho Lee ),( Don-sik Ryu ),( Ki-hoon Lee ),( Yunhee Kim ),( Dong-hoon Lee ) 한국폐기물자원순환학회 2010 APLAS Vol.2010 No.0

Reduction-Sensitive, Robust Vesicles with a Non-covalently Modifiable Surface as a Multifunctional Drug-Delivery Platform

Park, Kyeng Min,Lee, Don-Wook,Sarkar, Bijay,Jung, Hyuntae,Kim, Jeeyeon,Ko, Young Ho,Lee, Kyung Eun,Jeon, Hyesung,Kim, Kimoon WILEY-VCH Verlag 2010 Small Vol.6 No.13

<P>The design and synthesis of a novel reduction-sensitive, robust, and biocompatible vesicle (SSCB[6]VC) are reported, which is self-assembled from an amphiphilic cucurbit[6]uril (CB[6]) derivative that contains disulfide bonds between hexaethylene glycol units and a CB[6] core. The remarkable features of SSCB[6]VC include: 1) facile, non-destructive, non-covalent, and modular surface modification using exceptionally strong host–guest chemistry; 2) high structural stability; 3) facile internalization into targeted cells by receptor-mediated endocytosis, and 4) efficient triggered release of entrapped drugs in a reducing environment such as cytoplasm. Furthermore, a significantly increased cytotoxicity of the anticancer drug doxorubicin to cancer cells is demonstrated using doxorubicin-loaded SSCB[6]VC, the surface of which is decorated with functional moieties such as a folate–spermidine conjugate and fluorescein isothiocyanate–spermidine conjugate as targeting ligand and fluorescence imaging probe, respectively. SSCB[6]VC with such unique features can be used as a highly versatile multifunctional platform for targeted drug delivery, which may find useful applications in cancer therapy. This novel strategy based on supramolecular chemistry and the unique properties of CB[6] can be extended to design smart multifunctional materials for biomedical applications including gene delivery.</P> <B>Graphic Abstract</B> <P>Biocompatible vesicles based on cucurbit[6]uril (CB[6]) can undergo surface modification by host–guest chemistry, internalization into targeted cells by receptor-mediated endocytosis, and triggered release of entrapped drugs in cytoplasm. They serve as a highly versatile multifunctional platform for targeted drug delivery. <img src='wiley_img_2010/16136810-2010-6-13-SMLL201000293-content.gif' alt='wiley_img_2010/16136810-2010-6-13-SMLL201000293-content'> </P>