S6K1 Phosphorylation of H2B Mediates EZH2 Trimethylation of H3: A Determinant of Early Adipogenesis

Yi, S.,Um, S.,Lee, J.,Yoo, J.,Bang, S.,Park, E.,Lee, M.,Nam, K.,Jeon, Y.,Park, J.,You, J.,Lee, S.J.,Bae, G.U.,Rhie, J.,Kozma, Sara C.,Thomas, G.,Han, J.W. Cell Press 2016 Molecular Cell Vol.62 No.3

S6K1 has been implicated in a number of key metabolic responses, which contribute to obesity. Critical among these is the control of a transcriptional program required for the commitment of mesenchymal stem cells to the adipocytic lineage. However, in contrast to its role in the cytosol, the functions and targets of nuclear S6K1 are unknown. Here, we show that adipogenic stimuli trigger nuclear translocation of S6K1, leading to H2BS36 phosphorylation and recruitment of EZH2 to H3, which mediates H3K27 trimethylation. This blocks Wnt gene expression, inducing the upregulation of PPARγ and Cebpa and driving increased adipogenesis. Consistent with this finding, white adipose tissue from S6K1-deficient mice exhibits no detectable H2BS36 phosphorylation or H3K27 trimethylation, whereas both responses are highly elevated in obese humans or in mice fed a high-fat diet. These findings define an S6K1-dependent mechanism in early adipogenesis, contributing to the promotion of obesity.

Salmonella Typhi, Salmonella Enteritidis 및 Salmonella Typhimurium균에 대한 Phage typing 및 antibiogram 분석

신영학,유정식,오경수,이점규,이상원,고운영,양병국 국립보건연구원 2000 국립보건원보 Vol.37 No.-

벼 육묘트레이를 이용한 어린 잎 채소 생육에 미치는 광 조절 영향

최기영 ( K. Y. Choi ),김시홍 ( S. H. Kim ),김재경 ( J. K. Kim ),유형주 ( H. J. Yoo ),김일섭 ( I. S. Kim ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2016 강원 농업생명환경연구 Vol.28 No.2

벼 육묘 트레이를 활용한 어린잎 채소 재배 가능성을 탐색하고자 재배 기간 중 차광 처리하여 다채, 청경채, 알파인배추, 청치마 상추 등 4종을 17일간 재배하였다. 차광 처리는 무차광 17일(NS), 차광 8일+무차광 9일 (S8NS9), 무차광 8일+차광 9일(NS8S9), 차광 17일(S17) 하였다. 처리에 따른 누적 광량은 NS>S8NS9>NS8S9>S17처리구 순으로 높았으나, 누적 온도는 처리에 따른 차이가 크지 않았다 차광 8일 째 어린잎채소 4종의 생육과 엽록소함량은 감소하였다. 광 조절 17일 째 어린잎채소 4종의 초장, 엽장, 엽폭, 경경, 생체중, 엽록소 함량 등의 생육은 무차광 NS 처리구에서 가장 높았다. 생육 초기 광조절에 따른 생육 후기 초장과 생체중 증가율이 어린잎채소 4종 모두 NS 또는 S8NS9 처리구에서 높았다. 따라서 벼 육묘장 다층 시스템에서의 공간 활용을 고려한 어린잎채소 재배시 차광이 많은 하부 공간은 생육초기에 활용하고, 수확기에는 광 확보가 이루어지는 상부에서 재배가 이루어짐이 적합하리라 본다. This study was conducted to investigate the effect of light control on growth of baby leaf vegetables (tatsoi, pak-choi, chinese cabbage ‘alpine’ and leaf lettuce ‘cheongchima’) in rice seedling tray. Light treatment controlled in non-shading (NS), shading for 8 days+non-shading for 9 days (S8NS9), non-shading for 8 days+shading for 9 days (NS8S9), and shading for 17 days (S17). Integrated solar radiation in NS was highest, and in S8NS9>NS8S9>S17 order. Integrated temperature did not shown difference among the treatments for growing periods. Growth and chlorophyll content of baby leaf vegetables were lowest in shading treatment for 8days. The highest growth characteristics (plant height, leaf length, leaf width, stem diameters, and fresh weight) and chlorophyll content of baby leaf vegetables was shown in NS. Increment rate of plant height and fresh weight of baby leaf vegetables were high in NS or S8NS9 as affected by light control for 8 days in early growth periods. These results suggest that we can utilize in low layer for early period and in high layer for harvest periods for baby vegetable production in rice seedling factory.

국내 분리 다제내성 장티프스균의 Plasmid 및 β-lactamase의 특성에 관한 연구

유정식,신영학,오경수,이점규,김기상 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Review of the subgenus Aethina Erichson s. str. (Coleoptera: Nitidulidae: Nitidulinae) in Korea, reporting recent invasion of small hive beetle, Aethina tumida

Lee, S.,Hong, K.J.,Cho, Y.S.,Choi, Y.S.,Yoo, M.S.,Lee, S. 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol. No.

<P>Four species of the subgenus Aethina Erichson sensu stricto are recognized in Korea, including two species new to Korea: Aethina (s.str) aeneipennis, and the small hive beetle (SHB), A. (s.str) tumida. This is the first report of the small hive beetle in the Far Eastern Asia, which has been recently spreading worldwide, causing big economic loss in beekeeping industry. Comments on external and genital structures, brief biology, illustrations of the genitalia of both male and female, and a key to species of the subgenus Aethina s. str. are provided. As SHB becomes worldwide issues in apiculture, the invasion history of A. tumida into Korea and its larval description are also provided. (C) 2017 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.</P>

A phase II study of everolimus (RAD001), an mTOR inhibitor plus CHOP for newly diagnosed peripheral T-cell lymphomas

Kim, S. J.,Shin, D.-Y.,Kim, J. S.,Yoon, D. H.,Lee, W. S.,Lee, H.,Do, Y. R.,Kang, H. J.,Eom, H. S.,Ko, Y. H.,Lee, S. H.,Yoo, H. Y.,Hong, M.,Suh, C.,Kim, W. S. Oxford University Press 2016 ANNALS OF ONCOLOGY Vol.27 No.4

<P>Everolimus plus CHOP is effective for newly diagnosed, chemotherapy-naive peripheral T-cell lymphoma patients with acceptable toxicity.Everolimus, an oral mTOR inhibitor, has single-agent activity against relapsed lymphomas. Thus, we carried out a phase II study of everolimus in combination with CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) as a first-line treatment for patients with peripheral T-cell lymphoma (PTCL) based on our phase I study results. Participants (n = 30) received CHOP with 5 mg everolimus per day from day 1 to 14 every 21 days for a total of six cycles. The primary end point was the overall response rate (ORR), which included complete response (CR) and partial response (PR) to this regimen. Immunohistochemistry was used to evaluate the expression of phosphatase and tensin homology (PTEN) and phosphorylated S6 kinase (pS6K) as a response. The objective response rate was 90% with CR (n = 17) and PR (n = 10). The CR rate was different among subtypes; angioimmunoblastic T-cell lymphoma (AITL, n = 3) had a CR whereas PTCL-not-otherwise specified and ALK-negative anaplastic large-cell lymphoma (ALCL) patients showed 63% (12/19) and 29% (2/7) of CR rate, respectively. This difference in CR rate among subtypes was associated with PTEN loss because PTEN loss was not seen in AITL but 33% of ALCL patients. The most common toxicity was hematological, with 80% of patients experiencing at least one event of grade 3/4 neutropenia, and 60% of patients had grade 3/4 thrombocytopenia. The everolimus plus CHOP was effective for PTCL patients, and its efficacy might be related with the preservation of PTEN.</P>

Progress in the development of heating systems towards long pulse operation for KSTAR

Kwak, J.G.,Bae, Y.D.,Chang, D.H.,Chang, D.S.,Hong, B.G.,Hwang, C.K.,In, S.R.,Jeong, S.H.,Jin, J.T.,Jung, K.S.,Kim, B.R.,Kim, J.,Kim, S.K.,Kim, T.S.,Lee, D.W.,Lee, K.W.,Oh, B.H.,Seo, C.S.,Seo, M.S.,Yoo International Atomic Energy Agency 2007 Nuclear fusion Vol.47 No.5

<P>Construction of the Korea superconducting tokamak advanced research (KSTAR) tokamak is in its final phase. For the long-pulse KSTAR discharges, the ion cyclotron range of frequencies (ICRF) and neutral beam injection (NBI) heating systems are expected to play important roles through a selective heating of ions and electrons, control of the plasma pressure and current profiles, a core fuelling and beam diagnostics for the KSTAR. In addition, the ICRF system is expected to be used for possible discharge cleaning and assisting in the tokamak startup. In this paper, the recent progress in the development of the ICRF and the NBI heating systems is described. The four-strap ICRF antenna has been successfully tested for a voltage up to 41 kV for a pulse length of 300 s (to 46 kV for 20 s) in a test chamber. A prototype KSTAR NBI system has been developed. At present, the system has successfully produced a 1 MW beam power for 200 s and a 3.5 MW output beam power for 4 s.</P>

보완대체요법에 대한 국내 논문분석

김희자,이경숙,이미현,정동신,유재순,한혜숙,정승은,문미숙,이순희,박미숙 충주대학교 2006 한국교통대학교 논문집 Vol.41 No.-

Purpose: The purpose of this study was to analyze a tendency of Korean research on complementary and alternative therapy. Method: The data were collected 55 researches on complementary and alternative therapy which were published in Korea from 1996 to 2004. Data were analyzed according to published year, type of research and published journal, type of study design, subject, and effective variable of therapy. Result: 1) The number of published research were 5 from 1996 to 1999, and 50 from 2000 to 2004. 2) The number of dissertation were 41 and published articles in journals were 14. 3) According to type of study design, experimental design was 51, survey was 3, and combined study of experimental and survey was 1. 4) The most subjects were surgery patients who were applied foot massage. 5) Foot massage was shown to be more effective for fatigue than others. Conclusion: It is important to construct scientific knowledge by analyzing effect of various complementary and alternative therapies to more nursing clients. Furthermore it is necessary to develop and apply appropriate nursing implementation used complementary and alternative therapy for client's nursing problem.

Detection of Genotype associated with Disease Activity and Development of Probe

Jang,S. I.,Yoo,S. K.,Im,M. K.,Kim,J. H.,Kim,W. S.,You,Y. O.,Lee,D. K.,Kim,K. J.,Kim,W. S. 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.4

질환성과 관련된 세균의 분포 및 유전자형을 탐색하고자 구강농양 및 골수염의 급성감염 혼자와 진료실 및 실험실의 정상인을 대상으로 시료를 채취하여 포도상구균을 분리 및 동정을 시행하고, 특성을 규명하였으며, plasmid 및 염색유전자를 분리하여 제한효소를 처리후 전기영동을 실시하고 분리된 plasmid로 탐색자를 제작하여 dot blot을 시행하였다. 대부분의 급성환자에서 분리된 포도상구균을 S. lugdunensis와 S. aureus이었으나, 진료실 및 실험실에서는 coagulase 음성 staphylococci가 분리되었다. 급성환자에서 분리된 포도상구균은 ampicillin과 penicillin에 내성을 보였다. 분리된 S. lugdunensis균주중 네 균주는 δ형의 용혈소를 생산하였다. Plasmid를 분리한 결과 S. lugdunensis균주중 세 균주는 약 6.5 kilobases이었으나 S. aureus는 약 4.3 kilobases 정도 크기의 band를 보였다. S. lugdunensis에서 분리된 plasmid로 제작한 탐색자로 dot blot를 시행한 결과 치과 영역에서 분리한 plasmid를 갖는 균주는 양성반응을 보였다. 염색체유전자의 유전자형을 분석한 결과 δ형의 용혈소를 생산한 네 균주의 S. lugdunensis는 유사한 유전자형을 보였다. 이러한 연구결과 질환의 진행에 S. lugdunensis가 중요한 역할을 하는 것으로 생각되고, 치과영역에 존재하는 plasmid는 공통적인 유전자 서열을 갖는 것으로 추정된다.

야구경기에서 타격순서별 출루와 득점에 관한 연구 : 추계대학 야구연맹전을 중심으로

김정주,유승희 경희대학교 체육과학연구소 1978 體育學論文集 Vol.6 No.-

In order to examine not only the details how batter's advanced by hitting a safety, or four ball, or deall ball but also score in each battering order, Yungnam, Kunkook, Donga, Inha, Hanyang, and Yonsei Universities were selected as subjects for this study, which were successful in pushing into the final league match in Fall Season Nation-Wide College Base-ball Championship held from September 14 till 18, 1977. The results obtained by the test being conducted carefully to those teams are as follows; 1. The details of batter's frequency in advancing to another base, which was sinvestigated on the percentage basis. (1)The ratio, having made it possible for the batters in each team to advance to another was revealed to be 35.6% and the ratio of opposite case was exposed to be 64.4%. Accordingly the ratio between the former and the latter was proved to be 1:2. (2)The ratio of each batter's hit was as follows; 1st batter's hit (40.2%), 4th batter's hit (42.7%), 7th batter's hit (39.8%), 9th batter's hit(39.1%). Generally, those batter's abve mentioned are in the state of being good and high level in hitting. 8th batter's hit(21.3%), 2nd batter's hit (28/3%). These batters were not good in the ratio of hitting. In battings per one game, the first batter hit 4.4 times. The nineth; 3.5 times, there-fore there was a noticeable difference being revealed in the ability of batting between 1st and 9th batter. However no distinct difference in battings was being found between 3rd and fourth batters. (3)The more detailed ratio of batters' activity throughout the whole games are recorded as follows; one base hit (16.2%), four ball (7%) two base hit (3.9%), home run (1.2%). Grounder out (24.3%), flyout(22.4%), strike out(13.3%), sacrifice batting(fly & bunt) (2.3%) can be regarded as the direct cause of their having been prevented from advancing to the 2nd base. (4) The ratio of the 4th batter's strike out was 12.9%, which was fourth in low level. (5) The ratio of batters'advancing frequency to 2nd base in each team are as follows; Kunkook(41.5%), Hanyang(39.5%), Yungnam(38.2%), Inha(32.4%), Yonsei(32.4%), Donga(29.5%) II. Score (1) Score being made in each battering order can be described as follows; 1st & 9th batter (17 points) 4th & 9th batter (11 points) 3rd batter (1Opoints) 8th batter (7 points) 2nd batter (5 points) (2) In the aspect of score being recorded throughout the whole games, we were able to get such results as follows. In such a case as a base hit(a one timer), the teams got 38 points (36.5%). In such a case as home run hit, 27 points (26%). In such a case as a two-base hit(a two-beggar), 18 points(17.3%), and by errors and a sacrifice batting, they got merely 6 points(5.85%). No score, being made by a three-base hit (a triple). (3) Mutual relation between the batting order and the position of fielders was recorded as follows; 1st batter; short stop (15 times) 2nd " ; 2nd base man (15 times) 3rd " ; left fielder (11 times) 4th " ; 1st base-man (12 " ) 5th " ; catcher (10 " ) 6th " ; pitcher (6 " ) 7th " ; 2nd baseman (7 " ) 8th " ; Pitcher (19 " ) 9th " ; center fielder(8 " ) The above data show most popular frequency of each batter's position being changed while they are defending.