Duplication or deletion of chromosome 17p11.2-p12 in CMT1 and HNPP peripheral neuropathy patients

Kim, Hyo-Sun,Jang, Young-Eun,Chung, Ki-Wha 公州大學校 基礎科學硏究所 2003 自然科學硏究 Vol.10 No.-

CMT와 HNPP 말초신경증은 가장 흔하게 발병하는 운동 및 감각 말초신경계의 선천성 질환이다. CMT1과 HNPP의 유전적 원인으로는 염색체 17p11.2-p12의 1.5 Mb 지역의 중복과 결실이 각각 50%이상과 70% 이상을 차지하는 것으로 알려졌다. 본 연구에서 한국인 CMT1 및 HNPP 환자를 대상으로 중복 및 결실을 조사하였는데, 31 CMT1 가족 중 13 가족에서 중복을 보였으며 (42%), 24 HNPP 가족 중 19 가족에서 결실을 보였다 (79%). 17p11.2-p12 중복을 보이는 CMT1A 환자가 HNPP 결실환자보다 발병 연령, 발병 기간, 근육 이양성 및 족 기형성에서 훨씬 심한 증상을 보였다. Charcot-Marie-Tooth disease (CMT) and hereditary neuropathy with liability to pressure palsies (HNPP) are most common inherited motor and sensory peripheral neuropathies. The majority of CMT1 patients have a 1.5 Mb duplication in chromosome 17p11.2 while most HNPP patients have a deletion of the same region. In the analysis of Korean CMT1 and HNPP patients, each HNPP deletion and CMT1A duplication was observed from 79% (19 families) among 24 HNPP families and 42% (13 families) among 31 CMT1 families. CMT1A duplication patients showed more severe clinical symptoms than HNPP deletion patients, when we examined oneset age, disease duration, muscular atrophy, foot deformity and scoliosis.

Korean English Teacher's Disempowerment in English-only Classes

Sung-Sam Hwang,Hyo-Sun Seo,Tae-Young Kim 한국사회언어학회 2010 사회언어학 Vol.18 No.1

Hwang, Sung-Sam, SeD, Hyo-Sun, Kim, Tae-Young. 2010. Korean English Teacher's Disempowerment in English-only Classes: A Case Study Focusing on Korea-specific Cultural Aspects. The Sociolinguistic journal of Korea 18(1). In this case study, we have focused on the disempowerment of a Korean English teacher (KET) in English-only classrooms. Six English as a foreign language (EFL) classrooms in a Korean high school were observed and analyzed by using interpretive discourse analysis. Out of the six classes, three representative classrooms were both video- and audio-taped. The results show that the English-only classes could weaken the KETs' power via three mechanisms: (a) calling the KETs' last name for choral greetings at the beginning of the class, (b) students' group solidarity and peer pressure between classmates, and (c) the students' excessively collaborative approach to peer assistance. It is argued that the exclusive English-only instruction disempowers the KETs' status and dismantles their vocational identity. In order to remedy the current situation, within a scaffolding supported by the eclectic use of first language where necessary for effective class management, KETs are empowered and overcome constraints imposed by the limited L2 oral competence of some non-native English teachers.

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

고등학생의 자기표현과 자아존중감 향상을 위한 독서요법효과분석

선효원,심혜숙 부산대학교 과학교육연구소 2006 교사교육연구 Vol.45 No.-

The purpose of this study is determine effects of the reading therapy program on the self-expression and self-esteem of boy students of academic high schools. For the purpose, this researcher surveyed 2nd-grade boy students of academic high schools located in Busan. All of the surveyed students, 170 in number, were asked to receive self-expression and self-esteem tests before the program is applied. Out of them, 30 who were low in both self-expression and self-esteem were selected. Then out of the thirty, 20 who wanted to participate in the program wore finally selected. Those 20 participants were divided into experimental and control groups, each consisting of 10 members. The reading therapy program used here was first developed by Watson(1994), and adapted by this researcher appropriately. The program was applied on the basis of 2 hours(120 min.) a session. Group activities under the program were made over 10 sessions totally, with 2 session a week based. The control group was not treated in any way over the same sessions. In the study, the following research questions were given. 1. Will the reading therapy program increase self-expression? 2. Will the reading therapy program improve self-esteem? Scales by Rakos & Schroeder were used as a device for measuring the subjects' self-expression. And Coopersmith's scale(1969) was applied to the measurement of the subjects' self-esteem. For data collected here, means of scores obtained from self-expression and self-esteem tests before the application of the program, similarities between the experimental and control groups, means of scores from such tests after the program and differences between the two groups were all processed through t-test. Results of the study can be summarized as follows. 1. The self-expression of the experimental group which participated in the reading therapy program was significantly higher than that of the control group, at the significance level of P < .05. 2. The self-esteem of the experimental group significantly improved more than that of the other group, at the significance level of P < .05. In conclusion, the reading therapy program improved self-expression and self-esteem significantly. This study was carried out on 2nd-grade boy students of academic high schools, so its results are difficult to be generalized. In future, therefore, it is needed to examine effects of the program on the self-expression and self-esteem o high schools for male, female and the both sexes and compare results of the examination.

졸·겔法에 의한 TiN 薄膜 製造에 關한 硏究

宣孝任,姜泰範,李奭鎬 상명대학교 논문집 1993 상명대학교논문집 Vol.31 No.-

The polymeric sols were prepared by hydrolysis and polymerization of titanium tetraisopropoxide in diethanolamine. TiO_2 coating films prepared by dip coating of alumina substrate with polymeric sols were heated at various temperature for 7 hours in NH_3 gas. TiN thin films obtained from TiO_2 were investigated by XRD. TiO_2 coating film on alumina substrate was converted into the TiN thin film by nitridation under the flow of NH_3 gas at 1100℃ for 7 hours.

[논문]재생골재의 광물학적 및 화학적 특성과 동결융해 저항성

전쌍순,이효민,진치섭 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

급속한 산업화와 인구증가 및 대량소비에 따른 페기물에 의하여 초래되는 지구 환경오염을 위한 사회시스템의 구축이 필요함을 암시한다고 볼 수 있다. 본 연구에서는 지금까지 주로 저급재료로 사용되던 재생골재의 구성광물,화학조성 및 물리화학적 특성과 알칼리-골재 반응성 여부를 조사하고, 재생굵은골재의 대체율에 따른 콘크리트 강도와 동결융해 저항성을 검토하여 재생골재의 재활용을 위한 기초자료를 제시하고자 한다. 본 연구에 사용된 재생골재는 천연골재에 비교하여 공학적 기초 물성치에서 현저히 큰 차이는 나타내지 않았으며, 이를 사용한 재생콘크리트의 공학적 특성도 보통 콘크리트의 기준에 근접하는 것으로 나타났다.

[논문]반응성 골재의 입자 크기와 입도가 모르타르의 알칼리-실리카 반응 팽창에 미치는 영향

전쌍순,이효민,진치섭 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

골재의 알칼리-실리카 반응성을 판정하는 방법으로 모르타르 바 법 (Mortar-Bar method) 이 일반적으로 사용되고 있다. 본 연구에서는 알칼리 골재 반응성을 판정하는 모르타르 바 법 중에서 촉진시험법인 ASTM C 1260 시험방법을 이용하여 반응성 골재를 판별하고l 판별된 반응성 골재의 입자 크기와 입도가 모르타르의 알칼리-실리카 반응 팽창에 미치는 영향에 관하여 검토하였다. 그 결과I 반응성 골재의 입자 크기 및 입도에 상관없이 반응성 골재의 총표면적이 커질수록 팽창률도 증대하여,알칼리-실리카 반웅에 의한 팽창과 반웅성 골재의 총표면적과의 사이에는 상관관계가 있는 것을 확인할 수 있었다.

근적외분광분석법을 이용한 우호아청심원액 중 사향 대체물질의정량

김선민,김효진 동덕여자대학교 종합약학연구소 2007 동덕약학연구지 Vol.11 No.-

IThe near infrared spectroscopy(NIRs) was applied to develop the qualty control of the WooHwangChongShimWon which has been important herbal medicine. To develope the calibration method for ℓ-muscone in ethanol solution, concentration ranges of 4.15~29.07 mM ℓ-rnuscone was prepared. Partial least squares regression (PLSR) was used for developing a calibration model over the spectral range 833~2498 nm. The comparison of ethanol and air as a reference material was also performed for the proper spectral acquisition condition. Determination of ℓ-muscone in commercial WooHwangChongShimWon solution products was tried. The concentration of 5.9~51.9 ㎛ ℓ-muscone in ethanol and 6.4~48.9 ㎛ ℓ-muscone in WooHwangChongShimWon solution was considered. The PLSR model showed a good correlation R²=0.995 and SEP= 0.08 mM between NIR value and the absolute ℓ-muscone value. This study showed that the rapid classification of WooHwangChongShimWon solution was successfully performed by NIR spectroscopy.

다른 환경에서 수확된 서양민들레 종자들의 발아 변이성에 관한 연구

임병선,양효식,조승원 木浦大學校 沿岸環境硏究所 1997 沿岸環境硏究 Vol.14 No.1

시간적으로 변화하는 환경에 대한 유전형들의 서로 다른 반응은 개체군내에서 그러한 유전자형들의 장기적인 유지활동에 일조 할 수 있다. 우리는 생물형들의 계절의존 적합도의 구성요인들이 개체군내에서 유전적인 다양성을 유지하는데 도움이 되는지를 조사하였다. 봄, 여름, 가을 및 겨울로 구분된 4계절 동안 채종된 종자들이 온도처리에 따라 실험되어졌다. 발아세와 발아율의 차이는 앞선 연구들의 결과와 같이 생물형중에서 발견되었다. 생물형들 사이에서 나타나는 생태적인 차이들은 온도처리에 대해 생물형적 특성이 나타났다. 시간을 통한 대조적인 계절적 형성의 생물형들의 유지는 만약 장기간 적합도의 값이 비슷하게 유지된다면 기대 되어 진다. Differential response of genotypes to temporal environmental heterogeneity may contribute to the long-term persistence of those genotypes within a population. We tested whether season-dependent fitness components of biotypes could be responsible for the maintain of genetic diversity within a population. Seeds collected for four season (spring, summer, autumn, winter) were experimented to temperature treatments(15℃, 20℃, 25℃, 30℃). Differences in germination velocities and rates were found among the Taraxacum officinale biotypes supporting previous findings. Ecological differences among biotypes were also detected with biotype-specific response to the temperature treatments. The persistence of biotypes of contrasting seasonal performance through time is expected if long-term fitness values remain similar.

비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)