Improvement of skin inflammation and moisturizing effect of UVB-exposed HaCaT cells of low molecular weight fucoidan obtained from Sargassum siliquastrum

Hyeong Nam Jeon,Ilekuttige Priyan Shanura Fern,Seo-Yeong Park,Kirinde Gedara Isuru Sandanuwa,Arachchige Maheshika Kumari Ja,Eui Jeong Han,Ginnae Ahn 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

The present study evaluated the effect of a low molecular weight fucoidan obtained from a celluclast extract of Sargassum siliquastrum (S. siliquastrum) against improvement of skin inflammation and moisturizing effect caused by ultraviolet B (UVB) exposure in HaCaT cells. First of all, we prepared the four kinds of polysaccharides (SSS1, SSS2, SSS3 and SSS4) from the celluclast extract of S. siliquastrum by a step gradient ethanol precipitation. Among them, SSS4 consisted of the higher sulfate content showed the higher antioxidant activities. Also, UVB protective effect confirmed that SSS4 reduced intracellular ROS production while improving cell viability. In addition, SSS4 inhibited the activation of MAPK and NFκB signaling in UVB-exposed HaCaT cells. Moreover, SSS4 significantly increased the amount of hyaluronic acid by modulating the expression levels of skin barrier-related proteins and moisturizing factors. This study suggested that SSS4 is a fucoidan and has potential therapeutic effects on skin damage caused by UVB exposure in HaCaT cells. This work (Grants No. 2020R1A6A3A 0109950011) was supported by National Research Foundation of Korea in 2021.

Description and Application of a Marine Microalga Auxenochlorella protothecoides Isolated from Ulleung-do

Hyeong Seok Jang(장형석),Nam Seon Kang(강남선),Kyeong Mi Kim(김경미),Byung Hee Jeon(전병희),Joon Sang Park(박준상),Ji Won Hong(홍지원) 한국생명과학회 2017 생명과학회지 Vol.27 No.10

단세포 녹조류 균주를 경상북도 울릉군 울릉도 거북바위 주변 조수웅덩이로부터 순수분리하여 형태적, 분자적, 및 생화학적 특성을 분석한 결과 옥세노클로렐라 프로토테코이드에 속하는 것으로 밝혀졌다. 본 종은 현재까지 한국에서 공식 기록이 없는 미기록종으로 옥세노클로렐라 프로토테코이드 MM0011 균주라고 명명하였으며, 생장, 지질/광합성 색소 조성 및 바이오매스 특성에 대해 조사를 실시하였다. 분리균주는 광범위한 온도(5-35°C)에서 생장할 수 있었으며 1.5 M 염화나트륨 농도까지 생존할 수 있었다. 가스크로마토그래프/질량분석기를 이용한 분석 결과, 본 균주에는 영양적으로 중요한 불포화지방산이 풍부한 것으로 나타났으며, 특히 리놀네산(27.6%) 및 알파 리놀렌산(37.2%)이 주요 지방산 성분으로 확인되었다. 따라서 본 토착 미세조류 균주는 어유 또는 식물성유를 대체할 수 있는 잠재적인 오메가-3 및 오메가-6 불포화지방산 원료가 될 수 있을 것으로 사료된다. 또한, 고부가가치 항산화 물질인 루테인이 보조색소로서 본 균주에 의해 생합성 되는 것으로 밝혀졌다. 일반성분분석 결과 MM0011 균주의 휘발성물질 함량은 85.6%였으며, 원소분석 결과 총 발열량은 20.3 MJ kg<SUP>-1</SUP>으로 나타났다. 또한 배지로부터 40.5%의 전질소와 27.9%의 전인을 각각 제거할 수 있어 향후 바이오연료 원료물질 생산과 오 · 폐수처리를 연계할 수 있는 가능성 역시 제시하였다. 추가적으로 MM0011 바이오매스는 높은 단백질 함량(51.4%)을 갖고 있어 우수한 동물사료의 원료가 될 수 있는 가능성도 보여주고 있다. 따라서, 본 균주는 미세조류 기반 생화학 물질 생산 및 바이오매스 원료로서 상업적인 이용 가능성이 높음을 시사한다. A unicellular green alga was axenically isolated from a tidal pool on Ulleung-do, Korea. Morphological, molecular, and biochemical analyses revealed that the isolate belonged to Auxenochlorella protothecoides. The current study is the first record of this species in Korea. The microalgal strain was named as A. protothecoides MM0011 and its growth, lipid and pigment compositions, and biomass properties were investigated. The strain is able to thrive in a wide range of temperatures (5~35°C) and to withstand up to 1.5 M NaCl. The results of GC/MS analysis showed that the isolate was rich in nutritionally important polyunsaturated fatty acids (PUFAs). Its major fatty acids were linoleic acid (27.6%) and α -linolenic acid (39.6%). Thus, this indigenous microalga has potential as an alternative source of ω3 and ω6 PUFAs, which currently come from fish and plant oils. Also, the HPLC analysis revealed that the value-added antioxidant, lutein, was biosynthesized as the accessory pigments by the microalga. A proximate analysis showed that the volatile matter content was 85.6% and an ultimate analysis indicated that the gross calorific value was 20.3 MJ kg<SUP>-1</SUP>. Since 40.5% of total nitrogen and 27.9% of total phosphorus were removed from the medium, respectively, it also has potential as a feedstock for biofuel applications which could be coupled to wastewater treatment. In addition, the biomass may also serve as an excellent animal feed because of its high protein content (51.4%). Therefore, A. protothecoides MM0011 shows promise for application in production of microalgae-based biochemicals and as a biomass feedstock.

동결분쇄에 따른 아로니아, 자몽, 서리태, 발아현미의 이화학적 특성

전형주 ( Hyeong-ju Jeon ),이일남 ( Il-nam Lee ),한예은 ( Ye-eun Han ),정호준 ( Ho-jun Jeong ),박하은 ( Ha-eun Park ),정주영 ( Ju-yeong Jung ),이진규 ( Jin-kyu Rhee ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.4

식품중의 영양성분을 잘 보존하고 섭취가 용이하며 소화가 잘 될 수 있는 가공방법의 최적화를 위하여 식품원료의 동결분쇄물과 일반분쇄물을 비교하였다. 단백질의 공급원으로 알려진 서리태는 동결분쇄한 것이 일반분쇄한 것에 비해 미세하나 에너지원으로서 영양성분 보존율(이하보존율)이 4.57% 높고, 미네랄은 큰 차이가 없었다. 특히, β-carotene의 경우, 동결분쇄가공분말에서 11.54% 높게 포함되어 있어 비타민 A의 전구물질인 공급에 유리함을 보였다. 아로니아의 총 안토시아닌은 동결분쇄가 453.49 mg으로 일반분쇄 158.98mg 보다 보존율이 높았다. 자몽에서 열에 약한 베타카로틴은 동결분쇄가 129.47%, 니아신은 439.39%로 동결분쇄가 보존율이 높았다. 발아현미는 동결분쇄에서 단백질, 탄수화물, 니아신이 107.74%, 103.87%, 156.52%로 각각 높았다. Protease에 의한 단백질 소화율은 일반분쇄에 비하여 아로니아가 120.5%, 서리태가 101.14%로 동결분쇄방법이 단백질 소화율이 높았다. 그러나, 자몽과 발아현미는 동결분쇄에서 94.06%와 93.14%로 소화율이 떨어졌다. We compared the ingredients present in freeze-dried ground food samples with those present in hot air-dried and ambient ground food samples to optimize processing conditions for preserving nutrients in and for improving the ingestion of different foods. The freeze-dried ground sample of black bean showed 4.57% higher retention rate than the ambient ground sample of black bean. Mineral content was also significantly different between these two samples. Moreover, the freeze-dried ground sample of black bean contained 11.54% higher content of β-carotene, a precursor of vitamin A, than the ambient ground sample of black bean. Total anthocyanin content was 453.49 mg in the freeze-dried ground sample of Aronia compared with 158.98 mg in the ambient ground sample of Aronia. Contents of β-carotene and niacin increased by 129.47% and 439.39%, respectively, in the freeze-dried ground sample of grapefruit. Retention rates of proteins, car-bohydrates, and niacin were 107.74%, 103.87%, and 156.52%, respectively, in the freeze-dried ground sample of germinated brown rice. Protein digestibility increased by 120.5% and 101.14% in the freeze-dried ground samples of Aronia and black bean, respectively, but did not increase in the freeze-dried ground samples of grapefruit and germinated brown rice.

제3발표장 : 정보처리 및 복합기술 분야 ; 안개수막장치에 의한 단동형 플라스틱 온실의 보온 효과

윤남규 ( Nam Kyu Yun ),김형권 ( Hyeong Kwon Kim ),백이 ( Yee Baek ),전종길 ( Jong Kil Jeon ) 한국농업기계학회 2013 한국농업기계학회 학술발표논문집 Vol.18 No.1

카메라를 이용한 고압 증기 배관 누설/진동 감시시스템 개발

전형섭(Hyeong-Seop Jeon),서장수(Jang-Su Suh),채경선(Gyung-Sun Chae),손기성(Ki-Sung Son),김세오(Se-Oh Kim),이남희(Nam-Hee Lee) 한국비파괴검사학회 2016 한국비파괴검사학회지 Vol.36 No.6

발전소나 석유화학 플랜트 구조물에 누설이 발생하면 인명 피해 및 경제적인 손실을 초래한다. 이러한 누설은 플랜트 배관의 진동으로 인한 피로파괴나 배관 감육으로 인해 발생한다. 플랜트 배관의 진동을 감시하기 위한 방법으로 주로 가속도센서나 레이저센서가 사용되지만 설치 및 운용의 어려움이 따르고 동시에 광범위 측정 시 비용 증가가 발생하게 된다. 이러한 문제점들을 해결하기 위해 최근 카메라를 이용한 누설 및 진동 변위 측정 방법에 대한 연구가 이루어지고 있다. 카메라를 이용한 누설 및 진동 변위 측정 방법은 설치가 간단하고 원거리 측정 및 넓은 범의의 측정이 가능한 장점을 가지고 있다. 따라서 본 연구에서는 카메라를 이용해 누설 및 진동 변위를 측정할 수 있는 시스템을 개발하였고 실험을 통해 성능을 검증하였다. Leakages at plant structures of power and petrochemistry plants have led to casualties and economic losses. These leakages are caused by fatigue failure of pipelines and their wall thickness. Vibration measurement methods for plant pipelines mainly use acceleration and laser sensors. These sensors are difficult to install and operate and thus lead to an increase in operational cost especially for wide area surveillance. Recently, measurements of leak and vibration displacements using cameras have attracted the interest of many researchers. This method has advantages such as simple installation, long distance monitoring, and wide area surveillance. Therefore, in this paper, we have developed a system that can measure the leakage and vibrational displacement by using a camera. Furthermore, the developed system was verified with experimental data.

Improving the Quality of Music on a Mobile Phone Using EVRC

Young Han Nam,Jae Woong Jeong,Tae Kyoon Ha,Yunho Jeon,Jae Soo Kim,Seop Hyeong Park 대한전자공학회 2003 ITC-CSCC :International Technical Conference on Ci Vol.2003 No.7

α-Synuclein Disrupts Vesicle Fusion by Two Mutant-Specific Mechanisms

Gyeongji Yoo,Hyeong Jeon An,Sanghun Yeou,Nam-Ki Lee 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.11

Synaptic accumulation of α-synuclein (α-Syn) oligomers and their interactions with VAMP2 have been reported to be the basis of synaptic dysfunction in Parkinson’s disease (PD). α-Syn mutants associated with familial PD have also been known to be capable of interacting with VAMP2, but the exact mechanisms resulting from those interactions to eventual synaptic dysfunction are still unclear. Here, we investigate the effect of α-Syn mutant oligomers comprising A30P, E46K, and A53T on VAMP2-embedded vesicles. Specifically, A30P and A53T oligomers cluster vesicles in the presence of VAMP2, which is a shared mechanism with wild type α-Syn oligomers induced by dopamine. On the other hand, E46K oligomers reduce the membrane mobility of the planar bilayers, as revealed by single-particle tracking, and permeabilize the membranes in the presence of VAMP2. In the absence of VAMP2 interactions, E46K oligomers enlarge vesicles by fusing with one another. Our results clearly demonstrate that α-Syn mutant oligomers have aberrant effects on VAMP2-embedded vesicles and the disruption types are distinct depending on the mutant types. This work may provide one of the possible clues to explain the α-Syn mutant-type dependent pathological heterogeneity of familial PD.

대퇴골 전치환술 받은 악성 골종양 환자의 생존인자와 합병증

조완형(Wan Hyeong Cho),전대근(Dae-Geun Jeon),송원석(Won Seok Song),박환성(Hwan Seong Park),남희승(Hee Seung Nam),김경훈(Kyung Hoon Kim) 대한정형외과학회 2020 대한정형외과학회지 Vol.55 No.3

목적: 대퇴골 전치환술은 사지재건술의 극단적 술식 중 하나이며 증례가 드물어 이에 대한 연구는 주로 술식 후의 합병증에 대한 분석이며 본 술식의 적응증에 대한 분석은 미미하다. 저자들은 대퇴골 전치환술 36예의 1) 종양적 문제로 치환술을 받은 환자의 생존에 관련된 예후인자, 2) 치환물 및 하지의 생존율, 3) 치환물을 3년 이상 추시한 예의 합병증, 최종 하지 상태 및 기능적 결과를 알아보고자 하였다. 대상 및 방법: 대퇴골 전치환술을 받은 이유에 따라 원발성 종양에 의한 경우(15예, 1군), 오인 수술 및 국소재발로 인한 경우(16예, 2군), 재건물 실패로 인한 경우(5예, 3군)의 세 군으로 분류하였다. 환자 생존인자 분석 항목은 나이, 성별, 전치환술 원인, 항암요법 전후 종양 부피 변화, 절제연, 국소재발이었다. 결과: 전체 36예의 5년 환자 생존율은 31.5%±16.2%였다. 종양과 관련된 적응증으로 치환술을 받은 31예의 5년 생존율은 21.1%±15.6%였다. 항암요법 전후 종양의 크기가 같거나 감소한 10예의 5년 생존율은 50.0%±31.0%로 크기가 증가한 13예의 0.0%±0.0%보다 유의하게 높았다(p=0.02). 광범위 절제연을 얻은 12예의 5년 생존율은 41.7%±27.9%로 변연부 절제연의 0.0%±0.0%보다 유의하게 높았다(p=0.03). 성별, 나이, 대퇴골 전치환술을 시행받은 원인, 치환술 후 국소 재발여부와 생존율과의 유의한 차이는 없었다. 치환물 36예에서 5년 생존율은 16.6%±18.2%였다. 하지의 10년 생존율은 85.9%±14.1%였다. 최종 추시상 종양인공관절을 유지한 것이 12예, 회전성형술은 2예, 슬관절 상부 절단술 1예, 관절고정술 1예였다. 대퇴골 전치환술 후 3년 이상 추시가 가능했던 16예 중 수술이 필요했던 주 합병증이 14예, 보존적 치료만 했던 저등급 감염이 2예였다. 수술한 환자 14예 중 감염이 10예(71.4%)예, 국소재발이 2예, 고관절 탈구, bushing 파손, 대퇴동맥 폐색이 각각 1예였다. 결론: 종양과 관련된 적응증으로 대퇴골 전치환술을 시행한 환자 중 항암요법 후 종양의 크기가 커지거나 절제연이 불량한 경우, 환자가 조기에 사망하는 경우가 많았다. 따라서 전치환술의 시행에 신중해야 할 것으로 생각된다. 장기 생존 환자에서도 감염률이 높고 기능적 결과도 만족스럽지 않으나 현재 적응증을 고려했을 때 대퇴골 전치환술은 사지보존을 위한 최선의 방법으로 보인다. Purpose: Total femoral replacement (TFR) is an extreme form of limb salvage. Considering the rarity of this procedure, reports have focused on the complications and a proper indication is unclear. This study analyzed 36 patients with TFR who were asked the following: 1) prognostic factors related to survival in patients who underwent TFR with a tumoral cause; 2) overall implant and limb survival; 3) complications, functional outcome, and limb status for patients surviving for more than 3 years. Materials and Methods: According to the causes for TFR, 36 patients were categorized into three groups: extensive primary tumoral involvement (group 1, 15 cases), tumoral contamination by an inadvertent procedure or local recurrence (group 2, 16 cases), and salvage of a failed reconstruction (group 3, 5 cases). The factors that may affect the survival of patients included age, sex, cause of TFR, and tumor volume change after chemotherapy. Results: The overall five-year survival of the 36 patients was 31.5%±16.2%. The five-year survival of 31 patients with tumoral causes was 21.1%±15.6%. The five-year survival of 50.0%±31.0% in patients with a decreased tumor volume after chemotherapy was higher than that of increased tumor volume (p=0.02). The five-year survival of 12 cases with a wide margin was 41.7%±27.9%, whereas that of the marginal margin was 0.0%±0.0% (p=0.03). The ten-year overall implant survival of 36 patients was 85.9%±14.1%. The five-year revisionfree survival was 16.6%±18.2%. At the final follow-up, 12 maintained tumor prosthesis, three underwent amputation (rotationplasty, 2; above knee amputation, 1), and the remaining one had knee fusion. Among 16 patients with a follow-up of more than three years, 14 patients underwent surgical intervention and two patients had conservative management. Complications included infection in 10 cases, local recurrences in two cases, and one case each of hip dislocation, bushing fracture, and femoral artery occlusion. Conclusion: Patients showing an increased tumor volume after chemotherapy and having an inadequate surgical margin showed a high chance of early death. In the long-term follow-up, TFR showed a high infection rate and the functional outcome was unsatisfactory. Nevertheless, this procedure is an inevitable option of limb preservation in selected patients.

자율주행로봇 플랫폼을 위한 ESP32 모듈 기반 인터페이스 보드 설계

전호남(Ho-Nam Jeon),이규영(Kyu-Young Lee),노형석(Hyeong-Seok Roh),김갑순(Gab-Soon Kim) 한국기계가공학회 2024 한국기계가공학회지 Vol.23 No.4

This paper explores the design and development of an interface board crafted for seamlessly integrating diverse electronic components and computers into autonomous mobile robots. The distinct communication protocols among these components often result in internal complexity when using a protocol converter. To address this issue, a communication interface board for the autonomous mobile robot platform was developed in this study, featuring the incorporation of the ESP32 module with built-in Wi-Fi functionality. This interface board comes equipped with communication conversion capabilities for various inputs, including analog, digital, SPI, RS485, and I2C communications. Additionally, a DC/DC converter circuit is configured to supply various direct current voltages, such as 5V and 3.3V. The designed interface board, based on the ESP32, underwent manufacturing, and communication experiments were executed with the central processing unit. The experimental results validate smooth data communication. These findings suggest that the designed interface board can be effectively applied to autonomous mobile robot systems.

Legendre 다항식을 이용한 주파수 응답 함수의 곡선접합과 모드 매개변수 규명

박남규(Park, Nam-Gyu),전상윤(Jeon, Sang-Youn),서정민(Suh, Jeong-Min),김형구(Kim, Hyeong-Koo),장영기(Jang, Young-Ki),김규태(Kim, Kyu-Tae) 한국소음진동공학회 2006 한국소음진동공학회 논문집 Vol.16 No.7

A measured frequency response function can be represented as a ratio of two polynomials. A curve-fitting of frequency responses with Legendre polynomialis suggested in the paper. And the suggested curve-fitting algorithm is based on the least-square error method. Since the Legendre polynomials satisfy the orthogonality condition, the curve-fitting with the polynomials results to more reliable curve-fitting than ordinary polynomial method. Though the proposed curve-fitting with Legendre polynomials cannot cover all frequency range of interest, example shows that the suggested method is quite applicable in a limited frequency band.