Preliminary Study on the Vacuum Protection Valve of the PEFP Beamline

LEE, HWA-RYUN,Park, B. S.,Kim, H. S.,Kwon, H. J.,Cho, Y. S. Korean Physical Society 2010 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.56 No.6

내시경적 조직검사로 진단된 위 분선충증 1 예

이석호,이화영,이규택,강인구,최규완,백승운,이종균,이준혁,고광철,이종철,오영륜,현재근,이풍렬,김재준,채종일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2

Strongyloidiasis is a parasitic disease caused by Strongyloides stercoralis which exists in two forms : the free living and parasitic forms. It exists in warm, moist climate in areas where there is frequent fecal contamination of the soil. After cutaneous invasion by the filariform larvae, petechial hemorrage, pruritus, papular rashes, edema, and urticaria occur. Infection commonly occurs in the proximal intestine of the gastrointestinal (G-I) tract but may extend from the stomach to the anus. Once the worm is established in the small intestine, the physical findings may include epigastric tenderness to palpation. The mucosal biopsy is an inefficient way of making the diagnosis because the worm is found in the biopsy specimen in only 2% of patients. Gastric strongyloidiasis is rare. We experienced a case of gastric strongyloidiasis diagnosed by the endoscopic biopsy and serologic test for parasite specific IgG antibody by micro-ELISA.

(−)-Nyasol, isolated from <i>Anemarrhena asphodeloides</i> suppresses neuroinflammatory response through the inhibition of I-κBα degradation in LPS-stimulated BV-2 microglial cells

Lee, Hwa Jin,Li, Hua,Chang, Hee Ryun,Jung, Hyeyoun,Lee, Da Yeon,Ryu, Jae-Ha Informa Healthcare 2013 Journal of enzyme inhibition and medicinal chemist Vol.28 No.5

<P>Microglial activation has been associated with neurodegenerative diseases by inducing the neuroinflammatory mediators such as nitric oxide (NO), TNF-α and IL-1β. (−)-Nyasol, a norlignan isolated from a medicinal plant <I>Anemarrhena asphodeloides</I>, showed anti-inflammatory potential in lipopolysaccharide (LPS)-activated BV-2 microglial cells. (−)-Nyasol inhibited the production of NO and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) and also the expression of inducible nitric oxide synthase and cyclooxygenase-2, which are responsible for the respective production of NO and PGE<SUB>2</SUB>. It also suppressed the mRNA levels of TNF-α and IL-1β in activated microglial cells. These effects of (−)-nyasol were correlated with the inactivation of p38 MAPK and the suppression of LPS-induced I-κBα degradation. Taken together, these results suggest that (−)-nyasol can be a modulator in neuroinflammatory conditions induced by microglial activation.</P>

유아교사의 발달단계에 따른 전문성 및 교사-유아 상호작용

이수련(Lee, Soo-Ryun),이정화(Lee, Jeong-Hwa) 한국유아교육·보육복지학회 2014 유아교육·보육복지연구 Vol.18 No.3

본 연구는 유아교사의 발달단계에 따라 전문성과 교사-유아 상호작용이 어떤 차이를 나타내는지를 알아보고자 실시하였다. 본 연구를 수행하기 위해 P시에 근무하는 어린이집과 유치원 교사 294명이 연구대상이 되었으며, 이들을 대상으로 배부된 설문지 중 263부가 최종 분석 되었다. 연구결과는 다음과 같다. 첫째, 유아교사의 발달단계에 따른 전문성 인식은 입문수용과 안일안주 단계보다 능력구축과 열중성장 단계에서 더 높은 것으로 나타났다. 둘째, 유아교사의 발달단계에 따른 교사-유아 상호작용은 입문수용과 안일안주 단계보다 능력구축 단계에서 더 높게 나타났다. 이와 같은 결과를 토대로, 교사발달단계에 따른 세분화된 지속적 교육과 훈련이 필요하며 특히 안일안주 단계의 교사들을 지원하기 위한 교사교육 및 정책이 요구됨을 제안하였다. The purpose of this study was to examine whether preschool teachers’ professionalism and teacher-child interaction would differ in terms of the teachers’ developmental stages. A total of 263 preschool teachers in P city responded to three kinds of questionnaires to measure teachers’developmental stages, professionalism, and teacher-child interaction. The collected data were analyzed using ANOVA and Scheffe test. The findings of this study were as followings: First, there was a significant difference on the teachers’professionalism according to the developmental stages. Both the teachers in the competency building stage and in the enthusiastic and growing stage showed higher scores on the professionalism than those in the induction stage and in the stable and stagnant stage. Second, there was a significant difference on the teacher-child interaction in terms of teacher career stage. The teachers in the competency building stage showed higher score on the interaction than those in a the induction stage and in the stable and stagnant stage. The implications for teacher education were discussed on the basis of the results.

유치원 교사의 배경변인과 멘토링 경험에 따른 멘토링의 인식과 실태 및 요구

이정화(Lee, Jeong Hwa),이수련(Lee, Soo Ryun),조부월(Cho, Boo Wall) 한국유아교육·보육복지학회 2011 유아교육·보육복지연구 Vol.15 No.1

본 연구의 목적은 유치원 교사의 멘토링에 대한 실태, 인식, 요구를 교사 배경변인뿐 아니라 멘토링 경험여부, 경험한 멘토링의 유형에 따라 구체적으로 분석해보는 것이다. 연구대상은 부산시 소재 유치원에 근무하는 초임 및 경력 교사 409명이었다 연구방법은 멘토링에 대한 인식과 실태, 요구를 묻는 설문조사를 통해 이루어졌고, 그 결과는 교사의 배경변인, 멘토링 경험여부, 경험한 멘토링의 유형에 따라 분석되었다. 그 결과를 보면, 첫째, 연령, 학력, 직위가 높을수록 멘토링에 대한 이해가 높고 멘토링 경험자가 많았다. 둘째 멘토링 미경험집단은 다양한 경험의 전수에 도움을 받고자 멘토링에 참가하고 교수학습지도, 생활지도에 도움을 줄 것으로 기대하였다. 그리고 멘토링 경험집단은 교육활동에 도움을 받고자 멘토링에 참가하였고, 자기능력이 개발되고, 교육활동에 관심이 많아졌으며, 인간관계가 좋아진 점이 멘토링의 가장 큰 보람이며, 좋은점이라고 밝혔다. 셋째, 멘토링 미경험 집단과 경험 집단간 멘토링에 대한 요구사항에는 차이가 있었다. 이러한 연구의 결 과는 멘토링 프로그램 개발 및 적용을 위한 기초자료로서 활용할 수 있을 것으로 기대된다. This study was conducted to study mentoring realities the teachers of young children in the field have experienced and their recognition and needs for the mentoring. A total of 445 teachers participated in this study and responded to a questionnaire about mentoring. The collected data were analyzed by Frequency and x2 analyses using SPSS 15.0 computer program. As a result of analyses, there were plenty of differences in the teachers' understanding of and needs for the mentoring as well as the mentoring realities according to their backgrounds variables(i.e., age, education, experience, and status), yes or no experience of mentoring, and the type of mentoring they have experienced.

생팥과 증자팥의 성분 및 생리활성 비교

이륜경 ( Ryun Kyung Lee ),김미선 ( Mi Sun Kim ),이예슬 ( Ye Seul Lee ),이만효 ( Man Hyo Lee ),이종화 ( Jong Hwa Lee ),손호용 ( Ho Yong Sohn ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.2

본 연구에서는 팥의 유용기능을 이용한 고부가가치 식품 개발을 목표로 무처리한 생팥 분말과 1차 자숙 후 고온 증자하고 동결건조하여 제조된 식용 팥 분말을 대상으로 에탄올 추출물을 조제하고, 각각의 유용성분 및 항산화, 항균, 항당뇨, 및 항혈전 활성을 평가하였다. 추출효율의 경우 생팥보다 증자팥이 약 1.2배 증가되었으며, 추출물의 총폴리페놀 함량도 증자팥이 1.2배 증가되었다. 그러나, 증자팥의 총플라보노이드 함량은 생팥 대비 30% 수준으로 감소하였으며, 총당 및 환원당 함량 역시 생팥 대비 27.9% 및 30.8% 수준으로 감소하였다. 항산화 활성은 생팥 및 증자팥에서 모두 우수한 음이온 및 양이온 소거능을 나타내었으나, 생팥이 증자팥보다 강력하였으며, 환원력 및 nitrite 소거능에서도 생팥 추출물이 우수하였다. 한편 α-amylase 저해활성은 생팥에서 우수하였으며, α-glucosidase 저해활성은 증자팥에서 상대적으로 우수하였다. 가장 특이한 활성은 항혈전 활성 평가에서 확인되었으며, 생팥 추출물은 매우 강력한 프로트롬빈 및 혈액응고인자 저해를 나타낸 반면 증자팥 추출물에서 는 거의 활성이 나타나지 않았다. 상기 결과는 생팥의 증자과정 중 유용성분의 소실 및 유용활성의 손실이 나타남을 의미하며, 향후 생팥의 적합한 삶기 공정, 열처리 및 건조공정의 개발이 필요하며, 특히 현재까지 대부분 폐기되고 있는 증자팥 제조단계의 팥 자숙액의 효율적인 이용에 대한 연구가 필요함을 제시하고 있다. 본 연구결과는 기능성 팥 음료 및 양갱 제조 등을 위한 팥 고부가가치 식품 개발 기본자료로 활용될 것이다. In the course of study for the development of functional food using red beans (azuki beans, Phaseolus radiatus L.), the ethanol extracts from raw-red bean (RRB) and boiled-red bean (BRB) were prepared, and the components and various biological activities of both were compared. It was observed that the extraction yield, and the total polyphenol content, of the BRB were 1.2 times higher than that of the RRB. However, the contents of total flavonoid, total sugar and reducing sugar in the BRB were 30, 27.9 and 30.8% respectively when compared with those of RRB. In relation to antioxidative activity, both RRB and BRB exhibited moderate DPPH anion, ABTS cation, and nitrite scavenging activities and reducing power, though in all cases RRB demonstrated stronger activities than BRB. The extracts of RRB and BRB did not reveal any antimicrobial activities. In a α- amylase inhibitory activity assay, RRB was higher than BRB, while BRB showed higher α-glucosidase inhibitory activity than RRB. A strong and particular activity was observed in an anti-thrombosis activity assay of RRB. The extract of RRB demonstrated strong inhibitions against prothrombin and blood coagulation factors, with moderate thrombin inhibition. However, the extract of BRB did not exhibit any significant anti-thrombosis activity. Our results indicate that RRB has different, but useful biological activities, and loss or elimination of the biologically active substances in RRB occurs during the production of BRB. Therefore, to develop more functional foods from red beans, a study of suitable boiling, heating and drying processes is essential, and the efficient re-use of boiled waste water from the boiling process is necessary. These results could be applied to the further development of functional red bean beverages and sweat red bean pastes.

Red Liriope platyphylla stimulated the insulin secretion through the regulation of calcium concentration in rat insulinoma cells and animal models

Hye-Ryun Lee,Ji-Eun Kim,Young-Ju Lee,Moon-Hwa Kwak,Dong-Soon Im,Dae-Youn Hwang 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.2

The aim of this study was to investigate the effects of Red L. platyphylla (RLP) on calcium and glucose levels during insulin secretion. To achieve this, alteration of insulin and calcium concentrations was measured in rat insulinoma-1 (INS-1) cells and animal models in response to RLP treatment. In INS-1 cells, maximum secretion of insulin was detected upon treatment with 200 μg/mL of RLP for 20 min. Nifedipine, an L-type calcium channel blocker, effectively inhibited insulin secretion from INS-1 cells. Regarding calcium levels, the maximum concentration of intracellular calcium in INS-1 cells was obtained by treatment with 100 μg/mL of RLP, whereas this level was reduced under conditions of 200 μg/mL of RLP. Further, RLP-treated INS-1 cells showed a higher level of intracellular calcium than that of L. platyphylla (LP), Korea White Ginseng (KWG), or Korea Red Ginseng (KRG)-treated cells. This RLP-induced increase in intracellular calcium was abrogated but not completely abolished upon treatment with 40 μM nifedipine in a dose-dependent manner. Furthermore, the insulin level was dramatically elevated upon cotreatment with high concentrations of glucose and RLP, whereas it was maintained at a low level in response to glucose and RLP co-treatment at low concentrations. In an animal experiment, the serum concentration of calcium increased or decreased upon RLP treatment according to glucose level compared to vehicle treatment. Therefore, these results suggest that insulin secretion induced by RLP treatment may be tightly correlated with calcium regulation, which suggests RLP is an excellent candidate for diabetes treatment.

산란-되튐 동시 측정 방법에 의한 박막 중 수소 정량법

이화련(Hwa-Ryun Lee),음철헌(Chul Hun Eum),최한우(Han-Woo Choi),김준곤(Joonkon Kim) 한국분석과학회 2006 분석과학 Vol.19 No.5

유아 주도적 협동게임이 유아의 친사회적 행동에 미치는 영향

이정화(Lee Jeong hwa),이수련(Lee So Ryun) 한국열린유아교육학회 2008 열린유아교육연구 Vol.13 No.2

본 연구의 목적은 유아 주도적 협동게임 활동이 유아의 친사회적 행동에 어떠한 형향을 미치는지 알아보는 것이다. 유아 주도적 협동게임이란 집단 내 팀 구성원들이 집단의 공동목표 성취를 위해 서로 돕고 즐거움을 느낄 수 있도록 유아들이 주도적으로 게임의 방법 규칙 역할 및 순서 등을 토의하고 계획한 후 적극적으로 참여하는 그룹게임을 의미한다. 연구대상은 경기도 소재의 두 개 초등학교 병설유치원에 재원중인 5세 유아 66명이었다. 유치원 한 곳은 실험집단으로 다른 한 곳은 비교집단으로 무선 선정하였다. 12가지의 그룹게임을 실험집단과 비교집단에 실시하였는데 실험집단 유아들에게는 유아 주도적 협동게임 방법을 비교집단에는 교사가 주도가 되는 일반적인 그룹게임 방법을 적용하였다. 그 결과 유아 주도적 협동게임은 교사 주도적 일반 그룹게임에 비해 유아의 유치원 적응능력 대인관계 형성능력 개인정서 조절능력 등 친사회적 행동에 유의미한 효과를 보이는 것으로 나타났다. The purpose of this study was to examine the effects of child-initiated cooperative group games on children's prosocial behaviors. 66five-year-old children attending two kindergartens in Kyunggi-Do participated in this study. The participants were randomly assigned to an experimental or a control group. The children in the experimental group took part in twelve 30-40 minute sessions of cooperative group games, while the control group did teacher-directed group games. Before and after activities, all children were individually tested on the 'Skill Situations Measure' by Goldstein, which was translated and adapted by Lee Won Young Park Chan Ok & Noh Young hee (1993). The data were analyzed in ANOVA using SPSS/PC+. As a result of analyses. the children in the experimental group participating in child-initiated cooperative group games showed significantly higher increments of 'kindergarten adaptability', 'interrelationship ability', and ontrol of personal emotions than their counterparts experienced in teacher-directed group games. These findings imply that child-initiated cooperative group games have a positive effect on young children's prosocial behaviors.

Construction and In vitro Study of a Prx 6/Luc Vector System for Screening Antioxidant Compounds in the Transgenic Mice

Young Ju Lee(이영주),So Hee Nam(남소희),Ji Eun Kim(김지은),In Sik Hwang(황인식),Hye Ryun Lee(이혜련),Sun Il Choi(최선일),Moon Hwa Kwak(곽문화),Jae Ho Lee(이재호),Young Jin Jung(정영진),Beum Soo An(안범수),Dae Youn Hwang(황대연) 한국생명과학회 2013 생명과학회지 Vol.23 No.2

Peroxiredoxin 6 (Prx 6)는 티올-특이적 항산화 단백질에 속하는 효소로서 산화적 스트레스로부터 세포를 보호할 뿐만 아니라 과산화물의 환원작용을 촉매한다. 본 연구에서는 인간 Prx 6의 promoter를 이용하여 항산화반응을 유발하는 추출물을 효과적으로 스크리닝하는 새로운 형질전환마우스를 개발하는 최종목적을 달성하기 위한 중간단계로서, hPrx 6/Luc 벡터를 개발하고, 이들 벡터의 안정적 발현과 성공적 반응성을 세포주를 이용하여 확인하고자 하였다. 이를 위해, 인간 Prx 6 promoter를 증폭하여 luciferase cDNA와 결합한 hPrx 6/Luc 벡터를 제조하였으며, 제조된 벡터를 제한효소 절단과 염기서열분석을 통해 확인하였다. hPrx 6/Luc 벡터는 NCI-H460 세포에 transfection한 후 인삼(KWG), 홍삼(KRG), 맥문동(LP), 홍문동(RLP)의 4가지 추출물을 처리하여 luciferase activity를 측정하였다. 그 결과, luciferase activity는 4가지 추출물에 의해 효과적으로 증가하였고, 특히 KRG과 LP를 처리한 그룹이 KWG과 RLP를 처리한 그룹보다 높았다. 또한, luciferase activity는 RLP 농도에 의존적으로 증가하였다. hPrx 6/Luc 벡터와 hPrx 6 mRNA반응의 차이를 비교하기 위해, 4가지 추출물을 처리한 후 hPrx 6 mRNA의 양을 RT-PCR로 분석하였다. 그러나, hPrx 6 mRNA의 양은 비록 고농도의 RLP 추출물에서는 약간의 증가가 관찰되었지만, 대조군에 비하여 4가지 추출물에서 유의적인 차이가 없었다. 한편, 4가지 추출물에 의한 superoxide dismutase (SOD) 활성의 변화는 비록 일부 차이는 있었지만 hPrx 6/Luc 벡터와 유사한 반응을 나타내었다. 따라서, 이러한 결과는 hPrx 6/Luc 벡터는 성공적으로 제조되었고, 세포내에서 안정적으로 발현하면서 항상화물질에 민감하고 정량적으로 반응할 수 있음을 제시하고 있다. 더불어, 이러한 세포주에서 확인결과를 바탕으로 형질전환마우스가 개발된다면, 항산화물질을 정량적으로 스크리닝하는 시스템으로 적용가능성이 매우 높음을 보여주고 있다. Peroxiredoxin 6 (Prx 6) is a member of the thiol-specific antioxidant protein family, which may play a role in protection against oxidative stress and in regulating phospholipid turnover. The aim of this study was to determine whether a human Prx 6/Luc vector was stably expressed and responded to antioxidants in a lung cell line (NCI-H460). To achieve this, the luciferase signal, hPrx 6 mRNA expression, and superoxide dismutase (SOD) activity were measured in transfectants with a hPrx 6/Luc plasmid after treatment with four antioxidant extracts, including Korea white ginseng (KWG), Korea red ginseng (KRG), Liriope platyphylla (LP), and red Liriope platyphylla (RLP). First, the hPrx 6/Luc plasmid was successfully constructed with DNA fragments of human Prx 6 promoter, amplified by PCR using genomic DNA isolated from NCI-H460 cells, and cloned into the pTransLucent reporter vector. The orientation and sequencing of the hPrx 6/Luc plasmid were identified with restriction enzyme and automatic sequencing. A luciferase assay revealed significant enhancement of luciferase activity in the four treatment groups compared with a vehicle-treated group, although the ratio of the increase was different within each group. The KRG- and LP-treated groups showed higher activity than the KWG- and RLP-treated groups. Furthermore, the luciferase activity against RLP occurred roughly in a dose-dependent manner. However, the level of endogenous hPrx 6 mRNA did not change in any group treated with the four extracts. The SOD activity was in agreement with the luciferase activity. Therefore, these results indicate that the hPrx 6/Luc vector system may successfully express and respond to antioxidant compounds in NCI-H460 cells. The data also suggest that the Prx 6/Luc vector system may be effectively applied in screening the response of hPrx 6 to antioxidant compounds in transgenic mice.