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세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구
이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1
Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.
홍택근,이용림,임무현,정낙현 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1
솔잎의 기능성에 관한 연구를 위하여 솔잎발효추출물(PFE)과 에탄올추출물(PE 80, PE 50)로 효소적 저해활성과 아질산염 소거작용에 대하여 연구하였다. Tyrosinase의 저해효과는 솔잎발효 추출물인 PFE가 솔잎 에탄올 추출물 PE 80과 PE 50에 비해 약 5∼38%정도 저해활성 효과가 높게 나타났다. XOase 저해효과는 PFE가 62.77%, PE 80이 64.90%, PE 50이 55.91%의 저해율을 나타내였으며 ACE저해효과는 PFE가 78.02%, PE 80이 69.82%, PE 50이 21.75%의 순으로 솔잎발효 추출물인 PFE가 가장 높은 저해효과를 나타내었다. 아질산염 소거작용은 솔잎추출물 모두 pH 3.0이하에서 높은 분해능력을 나타내였다. 유기산 함량 분석 결과, PFE, PE 80, PE 50의 세 시료 모두 항산화작용에 관여하는 ascorbi acid가 가장 많이 함유되어 있었으며, 시료별 ascorbic acid의 함량은 솔잎 발효추출물인 PFE가 가장 많이 함유되어 있는 것으로 나타났다. Effects on the physiological functionality, such as tyrosinase, xanthine oxidase, angiotensin converting enzyme and Nitrite scavenging ability were also observed by pine needle fermentation extract(PFE) and the difference in the consistency of pine needle ethanol extracts(PE 80, PE 50) was found. In the inhibition effect on tyrosinase, PFEP showed 5-38% higher than that of PE 80 and PE 50. In the inhibition on XOase, PFE, PE 80 and PE 50 showed 62.77%, 64.390%, 55.91% respectively. In the inhibition effect on ACE, PFE, PE 80 and PE 50 showed 78.02%, 69.82% and 21.75% respectively. Among these, PFE showed the highest ACE inhibition effect. In the inhibition effect on nitrite scavenging anility, the pine needle extracts showed a high effect in pH 3.0. As the result of the research using HPLC for the organic and, all the samples(PFE, PE 80, PE 50) showed higher contents of the ascorbic acid concerned with the effect of the antioxidative. PFE showed the highest contents of the ascorbic acid.
( Taek Geun Lee ),( Seung Hyun Moon ),( Hyun Hwang Bo ),( T Aegwang Kwon ),( Hyun Ho Son ),( Sook Kyung Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Epinephrine can relieve severe symptoms of urticaria. There has been no previous study on the continuous infusion of epinephrine in uncontrolled acute urticaria. Objectives: This study was aimed to assess the efficacy and safety of continuous infusion of low-dose epinephrine for severe acute urticaria. Methods: From August 2010 to July 2014, 34 severe acute urticaria patients who were uncontrolled symptoms by conventional treatment, were treated with continuous infusionof low-dose epinephrine. They received 1mg epinephrine HCl mixed with 1L of normal saline for 40cc/hour. Blood pressure were checked on 0, 30, 60 and 90 minutes after onset of epinephrine. Results: Low-dose epinephrine significantly improved the clinical symptom. The mean duration of clinical symptom after epinephrine was 3.64 days. Adverse effects were observed in 2(5.8%) patients, and were palpitation & chest discomfort. The average variation of blood pressure after continuous infusion of epinephrine was -3.23mmHg. There were no significant side effects to suggest stopping epinephrine injection. Relapse of urticaria after injection of epinephrine was occurred at 1(2.9%) patient. Of the 34 patients who were followed for at least 1 month after completion of epinephrine injection, 31(91.1%) patients weresymptom free, and 3(8.8%) patients used antihistamine. Conclusion: This study shows that continuous injection of low-dose epinephrine is an efficacious and safe treatment option for treating acute urticaria.
Lee, Jung Hyo,Kim, Dai Geun,Jang, Geun Seok,Lyoo, Won Seok,Park, Won Ho,Lee, Minjung,Lee, Taek Seung American Scientific Publishers 2010 Journal of nanoscience and nanotechnology Vol.10 No.10
<P>We have synthesized APBT and APTBT containing benzothiadiazole units by Suzuki cross-coupling reaction with good yield. The polymers showed blue emission colors in aqueous solutions, while long wavelength shift was observed in the solid state due to facilitated exciton migration. APBT and APTBT are water-soluble and highly-fluorescent conjugated polymers with negatively charged sulfonate side chains and thus they can be electrostatically assembled with oppositely charged polyelectrolyte such as cationic polymer, poly(dimethyldiallylammonium chloride) (PDAC) via layer-by-layer (LbL) deposition technique on a glass slide. According to the increased the number of bilayer, we found that the assembled film exhibited larger enhancement of the long wavelength emission relative to the blue emission, due to the increased excition migration.</P>
CASE REPORT : Cutaneous and Systemic Plasmacytosis Associated with Renal Amyloidosis
( Taek Geun Lee ),( Woo Seok Jeong ),( Seung Hyun Moon ),( Hyun Hwangbo ),( Sook Kyung Lee ),( Dong Ryeol Lee ),( Tae Gwang Kwon ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.6
Cutaneous and systemic plasmacytosis (CSP) is a rare disorder of unknown etiology characterized by cutaneous polyclonal plasma cell infiltrates associated with various extracutaneous involvement and polyclonal hypergammaglobulinemia. Here, we report on a 54-year-old male patient with chronic renal insufficiency who presented with disseminated reddish-brown macules and plaques on the face and trunk. In our evaluation, he was found to have lymphadenopathy, polyclonal hypergammaglobulinemia; benign plasma cell infiltration involving the skin, bone marrow, and retroperitoneal area; and renal amyloidosis. To the best of our knowledge, this is the first reported case of CSP associated with renal amyloidosis. (Ann Dermatol 27(6) 759∼762, 2015)
Effect of Crystalline Phase on Dynamic Deformation Properties of Zr-Based BMG Alloys
Lee, Dong Geun,Lee, Yont Tai,Yeon, Jong Taek,Kim, Jeoung Han,Park, Nho Kwang,Lee, Sung Hak Trans Tech Publications, Ltd. 2007 Materials science forum Vol.539 No.-
<P>Dynamic compressive tests were conducted on Zr-based amorphous alloys and amorphous matrix composite containing dendritic β phases. Dynamic compressive tests were conducted using a compressive Kolsky bar and then the test data were analyzed in relation to microstructure and fracture mode. Under dynamic loading, the maximum shear stress and ductility of the amorphous alloy and composite were considerably lower than those under quasi-static loading because of the decreased resistance to fracture. Deformation under dynamic loading lowered strain and compressive strength because of reduced fracture resistance, and the alloy containing dendritic β phases showed better compressive strength and ductility than the monolithic alloy.</P>
이석호,이화영,이규택,강인구,최규완,백승운,이종균,이준혁,고광철,이종철,오영륜,현재근,이풍렬,김재준,채종일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2
Strongyloidiasis is a parasitic disease caused by Strongyloides stercoralis which exists in two forms : the free living and parasitic forms. It exists in warm, moist climate in areas where there is frequent fecal contamination of the soil. After cutaneous invasion by the filariform larvae, petechial hemorrage, pruritus, papular rashes, edema, and urticaria occur. Infection commonly occurs in the proximal intestine of the gastrointestinal (G-I) tract but may extend from the stomach to the anus. Once the worm is established in the small intestine, the physical findings may include epigastric tenderness to palpation. The mucosal biopsy is an inefficient way of making the diagnosis because the worm is found in the biopsy specimen in only 2% of patients. Gastric strongyloidiasis is rare. We experienced a case of gastric strongyloidiasis diagnosed by the endoscopic biopsy and serologic test for parasite specific IgG antibody by micro-ELISA.
Lee, Myungmo,Lee, Young-Ju,Park, Eunyoung,Park, Yohan,Ha, Min Woo,Hong, Suckchang,Lee, Yeon-Ju,Kim, Taek-Soo,Kim, Mi-hyun,Park, Hyeung-geun The Royal Society of Chemistry 2013 Organic & biomolecular chemistry Vol.11 No.12
<P>An efficient enantioselective synthetic method for the synthesis of (2<I>R</I>)-5-phenyl-2-alkylproline <I>tert</I>-butyl esters was reported. The phase-transfer catalytic alkylation of <I>tert</I>-butyl-5-phenyl-3,4-dihydro-2<I>H</I>-pyrrole-2-carboxylate in the presence of chiral quaternary ammonium catalysts gave the corresponding alkylated products (up to 97% ee). The following diastereoselective reductions afforded chiral 5-phenyl-2-alkylprolines which can be applied to asymmetric synthesis as organocatalysts or synthesis of biologically active proline based compounds, such as chiral α-alkylated analogues of (+)-RP66803, as potential CCK antagonists.</P> <P>Graphic Abstract</P><P>An efficient enantioselective synthetic method for the synthesis of (2<I>R</I>)-5-phenyl-2-alkylproline <I>tert</I>-butyl esters was reported. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3ob27089k'> </P>