Di-(2-ethylhexyl) phthalate (DEHP)를 처리한 흰쥐 호르몬 및 정소 미세구조 변화

김다함,김완종 순천향대학교 기초과학연구소 2006 순천향자연과학연구 논문집 Vol.12 No.2

Di-(2-ethy1hexyl) phthalate (DEHP) has been commonly used as a plasticizer in polyvinyl chloride resins for manufacturing flexible vinyl products. The toxicity of DEHP has been the topic of extensive research, especially concerning endocrine disrupting properties. This study was carried out to investigate the effects of DEHP on the ultrastructural changes of the spermatogenesis in the rat. And also, some hormones in serum were measured to evaluate the physiological effects of DEHP. The rats were orally intubated with either corn oil (experimental control) or 0.5㎎, 1.5㎎ and 4.5 ㎎ DEHP/㎏/day in 0.5㎖ of corn oil for 15 days before sacrificing and sampling. DEHP reduced the growth of the testes, inhibited spermatogenesis and altered ultrastructural features of most cell types of the rat testis depending on the dosage treated. Briefly, Differentiations of the Leydig cells, the Sertoli cells and the developing germ cells seemed to be impaired. DEHP increased the level of LH(ICSH), but lowered the level of testosterone. The detrimental effect of DEHP on spermatogenesis seems to be derived from the decreased level of testosterone by the Leydig cells, followed by the weak activity of the Sertoli cells and the germ cells, In conclusion, DEHP inhibited the spermatogenesis via lowering testosterone in the rats. The results suggest that DEHP could show properties of endocrine disrupter impairing the spermatogenesis and has effects on the ultrastructures in rat

절식에 의한 흰쥐 췌장의 기능 및 구조 변화

김다함,신길상,김완종 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

Ultrastructural characteristics of the pancreatic acinus and the islet of Langerhans of Sprague-Dawley rats after 4-day fasting were observed. Food-restricted rats showed a low glucose concentration of blood. On transmission electron microscopy, acinar cells had well developed rough endoplasmic reticulum, Golgi complex and zymogen granules in control groups. In case of experimental groups, ecocytosis of zymogen material into the lumen obviously decreased, distribution of zymogen granules in cytoplasm was eccentric, and swellings of rough endoplasmic reticulum and nuclear envelope were observed. And, in control groups, many capillaries were present near islet of Langerhans and insulin secreting B cells or glucagon secreting A cells were observed. Meanwhile, ultrastructural changes including the swellings of nuclear envelope and rough endoplasmic reticulum and the decrease of secretory granules of endocrine cells in experimental groups. These results suggest that food deprivation altered the ultrastructure and function of pancreas in rat.

축구 로봇의 전략 알고리즘 개선

김재헌,이대훈,이성민,최환도,김중완 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

This paper presents an strategy algorithm of a soccer robot. We simply classified strategy of soccer robot as attack and defense. We use DC-motor in our Soccer Robot. We use the vision system made by MIRO team of Kaist and Soty team for image processing. Host computer is made by Pentium III. The RF module is used for the communication between each robot and the host computer. Fuzzy logic is applied to the path planning of our robot. We improve strategy algorithm of soccer robot. Here we explain improvement of strategy algorithm and fault of the our soccer robot system.

중국거주 조선족 여성의 영적 안녕정도가 정신건강에 미치는 영향

정성덕,이종범,김진성,서완석,배대석,박순재,주열,염형욱,김승원,김구묘,안영록,황대홍,표미자,조창열,정태길 영남대학교 의과대학 2004 Yeungnam University Journal of Medicine Vol.21 No.2

자아초월 정신의학이 체계화되면서 영성(spirituality)은 인성의 한 부분이라는 이론이 대두되었는데 이 영성은 정신건강에 큰 영향을 미친다고 했다. 인간의 성향 중 영적 안녕 정도를 파악하여 그 정도가 정신건강 중 허위성 경향, 정신병적 경향 및 불안과 우울 경향 등에 어떠한 영향을 미치는지를 조사하고저 본 연구를 실시하였다. 대상은 중국의 연변 조선족 자치구에 거주하는 여성 400명을 대상으로 하여 한국판 영적 안녕척도를 사용하여 영적 안녕정도를 파악하였다. 영적 안녕척도의 총점과 이 척도의 2개 하위 척도인 종교적 안녕과 실존적 안녕 척도 양자로 평가한 성적이 불안-우울통합척도와 정신분열증 척도 및 허위성 척도로 평가한 성적에 미치는 영향을 검증하기 위하여 상관관계분석 및 회기분석을 실시하였다. 그 결과는 다음과 같다. 1) 영적 안녕척도로 평가한 조선족 여성의 총점은 68.29로 한국의 기독교 여성이 평가한 100.65보다 훨씬 낮은 점수였다. 2) 불안·우울통합척도로 평가한 총점은 44.88로 연변노인이 평가한 점수와 일개지역의 한국농촌주민이 평가한 점수와 비슷한 결과였다. 3) 허위성 척도로 평가한 성적은 평균 74.57로 70점 이상이 86%(344명)이었으나 영적 안녕 성적과 허위성 성적 간에는 유의한 상관이 없었다. 4) 영적 안녕총점은 정신분열증 척도로 평가한 정신병적 경향에 유의한 영향을 미치지 않았으나 종교적 안녕하위척도의 성적은 정신병적 경향을 높여준데 비하여 실존적 안녕하위척도의 성적은 정신병적 경향을 낮게 해주었다. 5) 영적 안녕척도의 총점 및 두 하위척도인종교적 안녕과 실존적 안녕은 불안과 우울에유의한 상관을 보였는데 영적 안녕총점이 높을수록 불안·우울 총점이 다소 낮아지는 경향을 보였다. 6) 종교적 안녕 하위척도와 불안과 우울과의 관계는 종교적 안녕점수가 높을수록 불안과 우울을 각각 다소 유의하게 높여주었으며 이에 비하여 실존적 안녕하위척도와 불안과 우울과의 관계는 실존적 안녕정도가 높을수록 불안 및 우울점수는 유의하게 낮아졌다. 이와 같은 성적을 미루어볼 때 연변에 거주하는 조선족 여성이 평가한 영적 안녕정도는 정신병적 경향과 불안 및 우울에 유의한 상관을 보였으면 이 척도의 하위 척도인 종교적 안녕정도는 정신건강에 부정적인 영향을 미치는 반면 실존적 안녕정도는 정신건강에 긍정적 영향을 미친 결과로 평가되었다. 이러한 결과는 공산주의 사회에서 실존적 안녕은 긍정적인 가치관으로 평가되는 반면 종교적 안녕은 정신건강에 부정적으로 작용한 것을 입증해 주었다고 하겠다. Background: Spirituality has been an important part of Transpersonal Psychology and is believed to have a large effect on the mental health because it has been systematized. The aim of this study was to determine the level of spiritual disposition on human beings along with its effects on one's mental health. Materials and Methods: The study targeted 400 women residing in Youn-Gil city of JiLin Prov., which is a district of the Cho-Sun tribe in China. Their spiritual well-being was studied using the Spiritual Well-being Scale-Korean Version. The spiritual well-being scale consists of 2 sub-scales of religious well-being and existential well-being. The study was evaluated using a lie scale, psychotic trend, and a combined anxiety-depression scale. The results were considered to be factors of one's mental health. The correlation between the spiritual well-being and each tendency was analyzed by regression analysis. Results: The total score of the Cho-Sun tribal women according to the spiritual well-being scale was 68.29 which was much less than the 100.65 of Korean Christian women. There was no significant correlation between the spiritual well-being and the Lie trend. However, it was found that 86%(344) of Cho-Sun tribal women scored above 70 in the Lie trend with a mean score of 74.57 which is higher than normal populations. Regarding the correlation between the spiritual well-being and psychotic trend, the psychotic trend became significantly higher when the religious well-being was at a high level. On the other hand, the psychotic trend became significantly lower when the existential well-being was at a high level. Regarding the correlation between the spiritual well-being and anxiety, the anxiety was significantly higher when the religious well-being was at a high level. However, the anxiety level was significantly low when the existential well-being was at a high level. Regarding the correlation between the spiritual well-being and depression, the depression level was somewhat significantly high when the religious well-being was at a high level. However, the depression level was significantly low when the existential well-being was at a high level. Conclusion: This study evaluated the effects of spiritual well-being on a person's mental health among Cho-Sun tribal women in Youn-Gil city of JiLIn Prov., P.R. of China. The results found that the religious well-being, which is a sub-scale of spiritual well-being, had negative effects while the existential well-being had positive effects on the mental health. These results proved that a person's religious disposition had negative effects on their mental health in a communitarian society.

The Intestinal Function and Constipation Improvement Effect of Kombucha Added with Purun Concentrate

Da-Bin Lee,Seung-Hyun Kim,Ha-Neul Kim,Da-Yeong Kim,Tae-Wan Kim 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Frog-inspired programmable nano-architectures for skin patches and medical applications

Da Wan Kim,Sang Yul Baik,Jungwoo Kim,Ji Won Kim,Changhyun Pang 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.2

International academics in Malaysian public universities: recruitment, integration, and retention

Chang Da Wan,Sirat Morshidi 서울대학교 교육연구소 2018 Asia Pacific Education Review Vol.19 No.2

Malaysia aspires for its higher education to be relevant, referred, and respected globally. To achieve that, the Malaysia Education Blueprint (Higher Education) 2015–2025 spelt out the need to search for talent beyond national boundaries, as well as develop the capacity to attract, develop and retain this talent. International academics, who are non-Malaysian citizens, also make up part of this pool of talent. This paper examines issues surrounding the recruitment, integration, and retention of international academics in three public research universities. In addition to understanding the trend for employing international academics, a typology of international academics is proposed to enable a more focused understanding of the challenges and constraints in recruiting, integrating, and retaining international academics in Malaysian public universities.

Academic culture in Malaysia: sources of satisfaction and frustration

Chang Da Wan,David W. Chapman,Ahmad Nurulazam Md Zain,Sigrid Hutcheson,Molly Lee,Ann E. Austin 서울대학교 교육연구소 2015 Asia Pacific Education Review Vol.16 No.4

This paper examines the sources of satisfaction and frustration among Malaysian academics across three types of higher education institutions (HEIs)—public research university, public comprehensive university and private non-profit university. Based on interview with 67 academics across six HEIs, there is a clear pattern and relationship between the sources of satisfaction and frustration and the types of institutions these academics were affiliated. The major sources of satisfaction are related to the nature of academic work, which includes supervising, mentoring, teaching and interacting with students, as well as conducting research and disseminating knowledge through publication. However, the major sources of frustration are predominantly related to the governance of higher education, resulting from unrealistic expectations, lack of transparency of the promotion and reward system, and a strong bureaucratic culture. The understanding of satisfaction and frustration has helped us to understand issues of morale, retention and, possibly, productivity of academics across these HEIs, and in turn, the understanding of these issues about academics has implications in understanding the governing structure of HEIs. Although this study is limited only to HEIs in Malaysia, the findings have wider implications in contributing to the understanding of governance and academic culture in the broader context of higher education.

RAW 264.7 대식세포에서 지질 다당류에 의한 미세먼지(PM<SUB>2.5</SUB>) 유발 염증 반응 증진에 미치는 ROS-NF-κB 신호 전달 경로의 역할

권다혜(Da Hye Kwon),김다혜(Da Hye Kim),김민영(Min Yeong Kim),황보현(Hyun Hwangbo),지선영(Seon Yeong Ji),박세광(Seh-Kwang Park),정지원(Ji-Won Jeong),김미영(Mi-Young Kim),이혜숙(Hyesook Lee),정재훈(JaeHun Cheong),남수완(Soo-Wan Nam),황혜 한국생명과학회 2021 생명과학회지 Vol.31 No.12

본 연구의 목적은 LPS가 처리된 RAW 264.7 대식세포에서의 염증 반응이 미세먼지(PM2.5)에 의해 더욱 증가될 수 있는지를 조사하는 것이다. 이를 위하여 LPS와 미세먼지(PM2.5)가 단독으로 처리되거나 LPS가 존재하는 조건에서 미세먼지(PM2.5)가 처리된 RAW 264.7 세포에서 염증 매개변수와 ROS의 생성 정도 및 염증 조절 유전자들의 발현 수준을 조사하였다. 본 연구의 결과에 의하면 세포 독성이 없는 범위에서 LPS가 처리된 세포에서 미세먼지(PM2.5)는 염증성 매개 인자(NO 및 PGE₂) 및 cytokine (IL-6 및 IL-1β)의 생성 수준이 각각의 단독 처리군에 비하여 매우 증가되었으며. 이는 이들의 생성에 관여하는 유전자들의 전사 및 번역 수준에서의 발현 증가와 연관성이 있었다. 또한, LPS가 처리된 RAW 264.7 세포에 미세먼지(PM2.5)가 노출되었을 때, 핵에서 NF-κB의 발현이 더욱 증가하였고, 세포질에서는 NF-κB 뿐만 아니라 IκB-α의 발현이 감소되었다. 이러한 결과는 LPS와 미세먼지(PM2.5)의 단독 처리에 비하여 동시 처리된 경우 NF-κB 신호계의 활성이 더욱 증가하여 염증성 유전자들의 전사 활성 촉진에 기여하였음을 의미한다. 나아가 LPS가 처리된 RAW 264.7 세포에서 미세먼지(PM2.5)에 의해 ROS 생성이 크게 증가되었지만 NF-κB 억제제는 ROS의 생성을 감소시키지 못하였다. 그러나, ROS 생성을 인위적으로 억제하였을 경우, 미세먼지(PM2.5)에 의해 증가된 염증 매개 인자의 발현 및 생성과 NF-κB의 활성화가 모두 감소되었다. 따라서, 본 연구의 결과는 LPS가 처리된 RAW 264.7 세포에서 미세먼지(PM2.5)에 의해 유도된 NF-κB 매개 염증 반응의 증가는 ROS 생성 의존적 현상임을 시사한다. The purpose of this study was to investigate whether the inflammatory response in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages could be promoted by particulate matter 2.5 (PM2.5) stimulation. To this end, the levels of inflammatory parameters, reactive oxygen species (ROS) and inflammation-regulating genes were investigated in RAW 264.7 cells treated with PM2.5 in the presence or absence of LPS. Our results showed that the production levels of pro-inflammatory mediators (nitric oxide and prostaglandin E₂) and cytokines (interleukin-6 and -1β) were significantly increased by PM2.5 stimulation in LPS-treated RAW 264.7 cells, which was correlated with increased expression genes involved in their production. In addition, when LPS-treated RAW 264.7 cells were exposed to PM2.5, nuclear factor-kappaB (NF-κB) expression was further increased in the nucleus, and the expression of inhibitor of NF-κB as well as NF-κB in the cytoplasm was decreased. These results suggest that the co-treatment of PM2.5 and LPS further increases the activation of the NF-κB signaling pathway compared to each treatment alone, thereby contributing to the promotion of transcriptional activity of inflammatory genes. Furthermore, although the generation of ROS was greatly increased by PM2.5 in LPS-treated RAW 264.7 cells, the NF-κB inhibitor did not reduce the generation of ROS. In addition, when the generation of ROS was artificially suppressed, the production of inflammatory mediators and the activation of NF-κB were both abolished. Therefore, our results suggest that the increase in the NF-κB-mediated inflammatory response induced by PM2.5 in LPS-treated RAW 264.7 macrophages was a ROS generation-dependent phenomenon.

생체 모사기반 웨어러블 진단 패치 개발

김다완(Da Wan Kim),정수연(Su Yeon Jeong),방창현(Chang Hyun Pang) 한국고분자학회 2014 고분자 과학과 기술 Vol.25 No.5