Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.

간호사의 이미지에 따른 간호전공만족도

가미정,김수미,김영경,남수진,오루미,육지수,이혜인,최미나,최혜민,허다은 이화여자대학교 간호과학대학 2010 이화간호학회지 Vol.- No.44

Purpose: The purpose of this descriptive study is to examine the correlation between nursing students’ perception of nurses and the level of their satisfaction with the major. Method: The survey was done with the total number of 192 nursing students from 10 different universities in S city selected by a convenience samplingmethod. In order to measure the perception of nurses, the measurement which was invented by Lee(1992) and revised by Jang(1998) was used. Also, the measurement designed by Shim, and modified by Kim(2007) was used for measuring of the students’ satisfaction in their major. The collected data was analyzed by SPSS 17.0. Results: 1.The most decisive factor of the conceptualization of the image of nurses for the nursing college students is their experience of meeting nurses as a patient. 2. Students reconsider the role of nurses by experiencing immediate relationships with nurses in field training. 3. Other factors of the survey such as the students’academic year, religion, the field training situation (individual experience, type of the hospital where they experienced the training, and a relationship with the nurse in charge of the training) or the existence of nurses among their family members do not affect the students’ satisfaction. 4. There is a positive correlation between the image of nurses of nursing college students and their satisfaction with the major(0.66). Conclusion: The positive image of nurses is directly related to the students’ satisfaction in the nursing major, which hugely affects on their academic achievements and decisions of career path. Therefore, there should be more efforts to increase the positive perception of nurses among nursing students.

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

직업훈련 프로그램이 장애인 직업준비도에 미치는 효과

이달엽,김다혜,이승욱 한국장애인재활협회 2004 재활복지 Vol.8 No.1

직업재활을 위해서는 무엇보다 장애인의 직업적 능력을 최대한으로 개발하고 적응시키기 위한 직업훈련이 필수적이며, 이러한 훈련을 통해 직업인으로서의 바탕이 되는 자질과 기능을 충분히 갖도록 해야 한다. 직업훈령에 있어서 단순한 직업적 기능의 훈련만으로는 효과를 거둘 수 없고 직업인으로서의 전인적 훈련이 필요하다. 따라서 장애인에게 적용되고 있는 직업훈련프로그램이 직업적응, 직업유지를 위해 가장 바탕이 되는 직업준비도에 어느 정도 영향을 미치는 효과가 있는지를 파악하여 직업훈련서비스 제공에 있어서 개선을 위한 기초자료로 활용하고자 하였다. 구체적인 연구문제로 직업훈련프로그램이 장애인의 직업태도, 대인관계 및 사회기술, 직업생활 유지에 필요한 직업에 대한 인식과 직업적응에 미치는 효과와 장애인 직업준비도 요인간의 영향에 대해서 알아보았다. 본 연구에서는 WPP, VALPAR 17, 진로태도성숙도, 자기효능감 측정 도구를 사용하여 대구직업전문학교 입학생 16명을 대상으로 4-5개월 동안 직업훈련프로그램 적용 전과 적용 이후 직업인성, 대인관계 및 사회기술, 직업인식, 자기효능감을 측정하여 직업준비도의 변화정도를 파악하였다. 직업준비도에 미치는 효과를 알아보기 위해 실시한 연구를 통해 나타난 결과들은 다음과 같다. 첫째, 직업준비도 요인 변화에 있어서 직업에서 요구하는 일에 적응하는데 중요한 요인이 되는 직업인성은 직업훈련프로그램 적용 이후 향상되었고, 직업준비를 위한직업인성 형성에 효과가 있는 것으로 나타났다. 둘째, 직업훈련프로그램 적용이 장애유형에 따른 직업준비도에 미치는 영향은 직업인성과 직업인식, 자기효능감에서 장애유형에 따라 효과의 차이가 발생하였다. 셋째, 직업준비도 요인간의 상관관계는 직업인성이 높으면 자기효능감도 높아지며, 또한 자기효능감이 높을수록 직업인식이 높아지는 것으로 나타났다. 넷째, 직업준비도 요인에 대한 독립변수들의 상대적 기여도를 알아보기 위해 실시한 회귀분석 결과에서 대상자들의 나이와 장애유형, 대인관계와 사회기술이 직업인성에 큰 영향을 미치는 것으로 나타났으며, 대인관계 및 사회기술은 아니와 직업인성이 큰 영향을 미치는 것으로 나타났다. 결론적으로 직업생활 유지에 있어서 직업적응을 위한 작업준비도를 높이기 위해서는 직업인성을 향상시킬 수 있는 직업훈련프로그램이 필요하며, 이러한 직업인성을 향상시킬 수 있는 방법은 대인관계 및 사회기술 영역에 비중을 둘 수 있는 직업훈련프로그램이 필요하다. 그리고 직업훈련프로그램의 직업유지나 직업적응에 대한 효과성 조사와 훈련교사들에 대한 교육도 지속적으로 이루어져야 하며, 이러한 것들이 프로그램구성이나 교과과정 작성 시 반영하는 연구설계가 필요하다. It is essential for rehabilitating persons with disabilities, to develop employment skills. To maximize vocational potential and abilities of persons with disabilities, it is required for them to obtain work attitudes and skills. However, the work skills training alone may not be enough for persons with severe disabilities, requiring them t o have work preparation services. Therefore, this study was aimed to understand whether the vocational training program provided by Dae-Gu Metropolitan Training Institute for Persons with Disabilities had impact on the improvement of both work skills and job readiness skills for them. Data gathered included the following areas. The effect of the disabled's vocational behaviour. the personal relations, the social skills, the vocational consciousness and the job readiness areas. The WPP, VALPAR 17, Course Attitude Maturity, Self-Efficacy Feeling Measurement Tool were utilized for the data gathering purposes. For vocational Rehabilitation, first of all, it is essential for disabled trainees to develop and adapt through individualized service provisors. They are required to have enough appropriate attitudes and skills which basic to average workers. For the vocational training, a simple vocational skills training alone may not be enough. Therefore, this study was aims to figure out whether the vocational training program which have applied to the trainees in Dae-Gu Vocational Training Institute (DGVTI) was effective or not. This training program was composed of the work adaptation training and job readiness training. Initial study questions posed are as follows. Whether this training program had effect on the research participants' vocational behaviours, personal relationship skills, social skills, the vocational consciousness and the job readiness skills. The current researcher utilized the WPP, VALPAR 17, Course Attitude Maturity. Self-Efficacy Feeling Measurement Tool. They measure types of vocational personality. personal relations and social skills, vocational and self-efficacy feeling in comparison with before and after the application of training program. The 16 research participants had exposed to 4-5 months in Dae-Gu Vocational Training Institute(DGVTI), operated by Korea Employment Promotion Agency for the Disabled (KEPAD). Statistical analyses focused on the means, standard deviations, and frequencies. Futher analyses included t-tests, correlation Analyses, regression analyses. The results of this study can be summarized as follows. First, in the job readiness skills area, after training, vocational personality (which is the important factor adapting to assignment demanding occupation) is improved, and reveals that the formation of vocational personality for job readiness and, in the vocational recognition and self-efficacy feeling are positively changed. Second. job readiness skills had effect to improve research participants' vocational adaptation skills, vocational recognition and self-efficacy feeling. Third. "correlation between job readiness factors" appeared that if scores of the vocational personality is high, then the self-efficacy feeling also rises. When the self-efficacy scores are high, the vocational recognition rises. Fourth, the results of regression analyses indicated that the age and the types of disabilities of the research participants had influenced on the occupational personality. Also, research participants who showed good personal relation skills and social skills had good job readiness. It is conclusive that the method of improving occupational personality would influence on personal relations and social skills. In conclusion, it is necessary for the rehabilitation practitioners to provide skills and interpersonal skills with their clients and trainees. The implication of this study is that there is a strong need to improve quality of vocational training programs.

Reports : Structure-activity relationships of the intramolecular disulfide bonds in coprisin, a defensin from the dung beetle

( Jae Ho Lee ),( Da Eun Lee ),( Hye Min Choi ),( Ha Hyung Kim3 ),( Ho Kim ),( Jae Sam Hwang ),( Dong Gun Lee ),( Jae Il Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.11

Defensins, which are small cationic molecules produced by organisms as part of their innate immune response, share a common structural scaffold that is stabilized by three disulfide bridges. Coprisin is a 43-amino acid defensin-like peptide from Copris tripartitus. Here, we report the intramolecular disulfide connectivity of cysteine-rich coprisin, and show that it is the same as in other insect defensins. The disulfide bond pairings of coprisin were determined by combining the enzymatic cleavage and mass analysis. We found that the loss of any single disulfide bond in coprisin eliminated all antibacterial, but not antifungal, activity. Circular dichroism (CD) analysis showed that two disulfide bonds, Cys20-Cys39 and Cys24- Cys41, stabilize coprisin’s α-helical region. Moreover, a BLAST search against UniProtKB database revealed that coprisin’s α-helical region is highly homologous to those of other insect defensins. [BMB Reports 2014; 47(11): 625-630]

The efficacy and toxicity of docetaxel in patients with recurrent or persistent epithelial ovarian cancer

Da Hye Ju,Yong Man Kim,Min Gyun Kim,Eu Gene Kim,Dae Yeon Kim,Dae Sik Seo,Jong Hyeok Kim,Young Tak Kim,Joo Hyun Nam 대한산부인과학회 2008 대한산부인과학회 학술대회 Vol.94 No.-

Effects of 365 nm Wavelength UV LED on Bacteria

Da-Hye Lee,Sun-Hee Park,Xiaotong Xu,Koth-Bong-Woo-Ri Kim,Min-Ji Kim,Ji-Hye Kim,Kwang-Su Cho,Go-Eun Kim,Ga-Ryeong Park,Dong-Hyun Ahn 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Differentiation of highbush blueberry (Vaccinium corymbosum L.) fruit cultivars by GC-MS-based metabolic profiling

Kim, Da Yeon,Kim, So-Hyun,Ahn, Hye Min,Lim, Sa Rang,Oh, Junsang,Choi, Seulgi,Lee, Hong-Jin,Auh, Joong-Hyuck,Choi, Hyung-Kyoon The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1

Highbush blueberries (Vaccinium corymbosum L.) are cultivated worldwide for their fruit with unique taste and potential health benefits. Blueray, Bluecrop, and Spartan are prominent among the various blueberry cultivars. We performed gas chromatography-mass spectrometry (GC-MS)-based metabolic profiling to differentiate the fruits of these three cultivars, and built an optimal partial least squares-discriminant analysis (PLS-DA) model to separate them. Amino acids, fatty acids, organic acids, phenolic compounds, and sugars were identified in the fruits. The optimized PLS-DA model for different cultivars of the fruits was obtained by selecting variables based on a variable importance in the projection (VIP) cut-off value of 1.0. Caffeic acid, aspartic acid, acetic acid, threonolactone, inositol, xylose, glucoside, linolenic acid, mannose, altrose, glycine alanine, and valine were found to be relevant and contributing compounds for differentiating cultivars. In addition, a hierarchical cluster analyses dendrogram pattern was correlated with the PLS-DA. This study suggested that GC-MS-based metabolic profiling coupled with multivariate statistical analysis could be used to differentiate the fruits of three major highbush blueberry cultivars.

Topography of the Submental Artery That Should Be Considered in Bleeding During Dentoalveolar Surgery

Kim, Da-Hye,Won, Sung-Yoon,Choi, Da-Yae,Kim, Hong-San,Jung, Ui-Won,Kim, Hee-Jin,Hu, Kyung-Seok Mutaz B. Habal, MD 2012 JOURNAL OF CRANIOFACIAL SURGERY - Vol.23 No.5

ABSTRACT: The purpose of the present study was to provide precise data regarding the branching pattern of the submental artery, which should be considered in occasions of bleeding during various dentoalveolar surgical procedures of the mandible, such as implant surgeries, tori removal, and iatrogenic injuries.Twenty-six embalmed adult hemifaces from Korean cadavers were used in this study. The vertical distance, horizontal distance, and diameter of the submental artery were measured from the site of the first premolar to the third molar. In cases where there was penetration of the mylohyoid muscle by the main branches of the submental artery, the same items were measured at that point.The vertical distance between the submental artery and the inferior border of the mandible decreased toward the premolar, whereas the horizontal distance from the lingual plate of the mandible increased gradually as it traveled in the anterior direction. The diameter of the artery narrowed slightly toward the premolar. The main branches of the submental artery perforated the mylohyoid muscle in 14 (54%) of the 26 specimens.As a result of this study, the submental artery is located higher from the inferior border and closer to the lingual plate of the mandible in the region of the molar than that of the premolar. Therefore, clinicians should be more careful of bleeding when performing surgery in the molar region compared with the premolar region. Where the mylohyoid muscle is perforated by the main branches of the submental artery, its point of insertion can be observed in diverse locations.

골밀도를 이용한 대퇴근위부 형태 변화의 상관관계 평가

김다혜(Da-hye Kim),고성진(Seong-Jin Ko),강세식(Se-Sik Kang),김정훈(Jung-Hoon Kim),김동현(Dong-hyun Kim),예수영(Soo-Young Ye),김창수(Changsoo Kim) 한국콘텐츠학회 2012 한국콘텐츠학회논문지 Vol.12 No.12

우리나라는 급격한 경제성장과 의료기술의 발달로 고령화 사회가 되면서 골다공증 발병이 증가하고 있다. 또한 서구화된 식생활로 암질환이 증가하였고 항암치료과 방사선 치료 등으로 인해 골다공증이 발생하게 된다. 골다공증은 골밀도의 감소로 인한 것으로 대퇴근위부의 형태학적 구조 변화와도 밀접한 관계를 가지고 있으며, 대퇴 근위부의 형태학적 구조 변화는 고관절 골절의 위험을 나타내는 요인이다. 본 연구는 T-score변화에 따라 정상, 골감소증, 골다공증으로 분류하여 각 군별로 대퇴 근위부의 형태학적 변화를 관찰하고 상관관계를 분석하였다. 조사대상은 부산지역의 D종합병원의 남녀 350명을 대상으로 연령, 성별, T-score변화로 질환을 구분하여 분석하였다. 결과는 연령, 성별에 따라 골다공증의 발병빈도는 유의한 차이를 보였고, T-score변화에 따른 질환 분류와 대퇴근위부 형태학적 특성의 7가지 파라미터로서 Cortical ratio calcar, Cortical ratio shaft, Hip/shaft Angle, Strength index, Section modulus, CSMI, CSA는 유의한 차이를 보였으며 골다공증과 높은 상관관계가 있었다. 그러므로 연구 결과는 T-score변화에 따른 대퇴근위부의 형태학적 변화를 상관관계 분석함으로써 골다공증을 진단하는데 대퇴 근위부의 형태학적 변화가 지표로서 활용가능하리라 사료되며, 향후에 대퇴근위부의 형태학적 파라미터가 골다공증 진단의 정확성을 높이고 예측인자로서 임상활용이 가능하리라 판단된다. Osteoporosis is increasing in Korea as it becomes an aging society with the rapid economic growth and the development of medical technology. Osteoporosis also develops due to chemo and radiation therapy of cancer which also increases owing to Westernized diet. Osteoporosis is caused by reduced bone density, has close relationship with the change of geometry of proximal femur, which is a factor of hip fracture risk. The purpose of this study was the analysis of the correlations of osteoporosis and the change of geometry of proximal femur, which was observed according to T-score variance. The 350 male and female patients are chosen from D hospital in Busan, who were classified by age, sex and T-score values (normal, osteopenia, and osteo porosis). The results show that the age and gender have significant difference in the incidence of osteoporosis; the disease classification according to T-score value has significant difference in the geometry of the proximal femur such as Cortical ratio calcar, Cortical ratio shaft, Hip/shaft Angle, Strength index, Section modulus, CSMI, and CSA, and is highly correlated with the incidence of osteoporosis. Therefore, the findings of this research is that the change of the geometry of the proximal femur could be used as an indicator in the diagnosis of osteoporosis, could enhance the accuracy of the diagnosis in the future, and could be used as a clinical predictive factors through the analysis of the correlations of T-score variance and the geometry changes of the proximal femur.