http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Han Gyung Kim,Woo Seok Yang,Jae Youl Cho 한국버섯학회 2014 버섯 Vol.18 No.2
The inhibitory activities of the Cordyceps pruinosa butanol fraction (Cp-BF) were investigated by determining inflammatory responses of lipopolysaccharide (LPS)-treated RAW264.7 macrophage cells and by evaluating HCl/ethanol (EtOH)-triggered gastric ulcers in mice. The molecular mechanisms of the inhibitory effects of Cp-BF were investigated by identifying target enzymes using biochemical and molecular biological approaches. Cp-BF strongly inhibited the production of NO and TNF-α, release of reactive oxygen species (ROS), phagocytic uptake of FITC-dextran, and mRNA expression levels of interleukin (IL)-6, inducible NO synthase (iNOS), and tumour necrosis factor-alpha (TNF)-α in activated RAW264.7 cells. Cp-BF also strongly down regulated the NF-κB pathway by suppressing IKKβ according to luciferase reporter assays and immunoblot analysis. Furthermore, Cp-BF blocked both increased levels of NF-κB-mediated luciferase activities and phosphorylation of p65/p50 observed by IKKβ overexpression. Finally, orally administered Cp-BF was found to attenuate gastric ulcer and block the phosphorylation of IκBα induced by HCl/EtOH. Therefore, these results suggest that the anti-inflammatory activity of Cp-BF may be mediated by suppression of IKKα and its downstream NF-κB activation. Since our group has established the mass cultivation conditions by developing culture conditions for Cordyceps pruinosa, the information presented in this study may be useful for developing new anti-inflammatory agents.
Anti-inflammatory functions of the CDC25 phosphatase inhibitor BN82002 via targeting AKT2
Kim, Han Gyung,Yang, Woo Seok,Hong, Yo Han,Kweon, Dae-Hyuk,Lee, Jongsung,Kim, Sunggyu,Cho, Jae Youl Elsevier 2019 Biochemical pharmacology Vol.164 No.-
<P><B>Abstract</B></P> <P>This study presents BN82002 as an anti-inflammatory drug candidate. It was found that BN82002 inhibited the production of nitric oxide (NO) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in RAW 264.7 cells and peritoneal macrophages that were activated by toll-like receptor (TLR) 4 ligand, lipopolysaccharide (LPS). BN82002 dose-dependently down-regulated mRNA levels of nitric oxide synthase, tumor necrosis factor-α, and cyclooxygenase-2. The nuclear translocation of nuclear factor (NF)-κB (p65 and p50) was also blocked by BN82002 in RAW265.7 cells stimulated by LPS. According to reporter gene assay performed with NF-κB construct, BN82002 clearly reduced increased level of luciferase activity mediated by transcription factor NF-κB in LPS-treated RAW264.7 cells and in MyD88- and AKT2-overexpressing HEK293 cells. However, BN82002 did not inhibit NF-κB activity in AKT1- or IKKβ-overexpressing HEK293 cells. NF-κB upstream signaling events specifically targeted AKT2 but had no effect on AKT1. The specific target of BN82002 was Tyr-178 in AKT2. BN82002 bound to Tyr-178 and interrupted the kinase activity of AKT2, according to a cellular thermal shift assay analysis of the interaction of BN82002 with AKT2 and an AKT2 mutant (Tyr-178 mutated to Ala; AKT2 Y178A). These results suggest that BN82002 could reduce inflammatory pathway by controlling NF-κB pathway and specifically targeting AKT2.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kim, Han Gyung,Choi, Subin,Lee, Jongsung,Hong, Yo Han,Jeong, Deok,Yoon, Keejung,Yoon, Deok Hyo,Sung, Gi-Ho,Lee, Seungihm,Hong, Suntaek,Yi, Young-Su,Kim, Jong-Hoon,Cho, Jae Youl Hindawi 2018 Evidence-based Complementary and Alternative Medic Vol.2018 No.-
<P><I>Celtis choseniana</I> is the traditional plant used at Korea as a herbal medicine to ameliorate inflammatory responses. Although<I> Celtis choseniana</I> has been traditionally used as a herbal medicine at Korea, no systemic research has been conducted on its anti-inflammatory activity. Therefore, the present study explored an anti-inflammatory effect and its underlying molecular mechanism using<I> Celtis choseniana </I>methanol extract (Cc-ME) in macrophage-mediated inflammatory responses.<I> In vitro</I> anti-inflammatory activity of Cc-ME was evaluated using RAW264.7 cells and peritoneal macrophages stimulated by lipopolysaccharide (LPS), pam3CSK4 (Pam3), or poly(I:C).<I> In vivo</I> anti-inflammatory activity of Cc-ME was investigated using acute inflammatory disease mouse models, such as LPS-induced peritonitis and HCl/EtOH-induced gastritis. The molecular mechanism of Cc-ME-mediated anti-inflammatory activity was examined by Western blot analysis and immunoprecipitation using whole cell and nuclear fraction prepared from the LPS-stimulated RAW264.7 cells and HEK293 cells. Cc-ME inhibited NO production and mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and tumor necrosis factor-alpha (TNF-<I>α</I>) in the RAW264.7 cells and peritoneal macrophages induced by LPS, pam3, or poly(I:C) without cytotoxicity. High-performance liquid chromatography (HPLC) analysis showed that Cc-ME contained anti-inflammatory flavonoids quercetin, luteolin, and kaempferol. Among those, the content of luteolin, which showed an inhibitory effect on NO production, was highest. Cc-ME suppressed the NF-<I>κ</I>B signaling pathway by targeting Src and interrupting molecular interactions between Src and p85, its downstream kinase. Moreover, Cc-ME ameliorated the morphological finding of peritonitis and gastritis in the mouse disease models. Therefore, these results suggest that Cc-ME exerted<I> in vitro</I> and<I> in vivo</I> anti-inflammatory activity in LPS-stimulated macrophages and mouse models of acute inflammatory diseases. This anti-inflammatory activity of Cc-ME was dominantly mediated by targeting Src in NF-<I>κ</I>B signaling pathway during macrophage-mediated inflammatory responses.</P>
Pro-Apoptotic Activity of 4-Isopropyl-2-(1-Phenylethyl) Aniline Isolated from Cordyceps bassiana
Kim, Mi Seon,Lee, Yunmi,Sung, Gi-Ho,Kim, Ji Hye,Park, Jae Gwang,Kim, Han Gyung,Baek, Kwang Soo,Cho, Jae Han,Han, Jaegu,Lee, Kang-Hyo,Hong, Sungyoul,Kim, Jong-Hoon,Cho, Jae Youl The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.4
Cordyceps species including Cordyceps bassiana are a notable anti-cancer dietary supplement. Previously, we identified several compounds with anti-cancer activity from the butanol fraction (Cb-BF) of Cordyceps bassiana. To expand the structural value of Cb-BF-derived anti-cancer drugs, we employed various chemical moieties to produce a novel Cb-BF-derived chemical derivative, KTH-13-amine-monophenyl [4-isopropyl-2-(1-phenylethyl) aniline (KTH-13-AMP)], which we tested for anti-cancer activity. KTH-13-AMP suppressed the proliferation of MDA-MB-231, HeLa, and C6 glioma cells. KTH-13-AMP also dose-dependently induced morphological changes in C6 glioma cells and time-dependently increased the level of early apoptotic cells stained with annexin V-FITC. Furthermore, the levels of the active full-length forms of caspase-3 and caspase-9 were increased. In contrast, the levels of total forms of caspases-3, caspase-8, caspase-9, and Bcl-2 were decreased in KTH-13-AMP treated-cells. We also confirmed that the phosphorylation of STAT3, Src, and PI3K/p85, which is linked to cell survival, was diminished by treatment with KTH-13-AMP. Therefore, these results strongly suggest that this compound can be used to guide the development of an anti-cancer drug or serve as a lead compound in forming another strong anti-proliferative agent.
Simultaneous Expression of the Protease Inhibitors in a Rice Blast-Resistant Mutant
Han, Chong U.,Lee, Chan-Hui,Choi, Gyung-Ja,Kim, Jin-Cheol,Ahn, Sang-Nag,Choi, Jae-Eul,Cha, Jae-Soon,Cho, Kwang-Yun,Lee, Seon-Woo The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.4
We have previously identified genes for four different protease inhibitors (PIs) that were induced upon rice blast infection in a rice blast resistant mutant SHM-11. Our expression analysis of the PIs indicated that induction of the PIs was the highest 24 hr after rice blast inoculation in the rice mutant SHM-11. Three PIs in the group of serine PIs were highly expressed while a cystein PI was weakly expressed upon rice blast inoculation. Four PIs were weakly induced 48 hr after pathogen inoculation in rice blast susceptible wild type rice plant. The simultaneous expression of three serine PIs was apparent from SHM-11 and two of them were induced in rice blast resistant Taebaegbyeo. One of them was induced in rice blast resistant Hwayeongbyeo while none of them were expressed in rice blast susceptible Nagdongbyeo and rice blast resistant Dongjinbyeo. Our results suggest that the expression of PI gene is rice cultivar specific and may be linked with the rice blast resistance in a specific rice mutant by the simultaneous expression of the PI genes.
췌장 낭종성 종양으로 오인되었던 부신 기원의 신경절성아세포종
한동수,이민호,김홍주,조윤주,최호순,윤병철,손주현,이오영,전용철,함준수,기춘석,박경남,박석규,임현철 대한소화기학회 2000 대한소화기학회지 Vol.36 No.4
Neuroblastoma, a tumor of the peripheral nervous system, is the most common extracranial solid tumor of childhood. Neurogenic tumor which includes neuroblastoma is classified into neuroblastoma, ganglioneuroblastoma and ganglioneuroma according to cytologic differentiation. Many cases of ganglioneuroblastoma which is the most rare tumor of neurogenic tumors have been reported during past few decades in U. S and European literature. However, in Korea, only 3 cases of ganglioneuroblastoma in adult have been reported. Recently, we experienced a case of ganglioneuroblastoma which was initially misdiagnosed as pancreatic cystic neoplasm by imaging study. A 27-year-old male patient admitted our hospital with chief complaint of left upper quadrant discomfort and intermittent vomiting. Imaging studies, such as abdominal sonogram, abdominal computed tomography, endoscopic retrograde cholangiopan creatography and angiogram revealed a cystic mass in pancreatic tail portion. The patient underwent mass excision, left nephrectomy and adrenalectomy. Diagnosis of ganglioneuroblastoma of adrenal origin was made on the basis of its histopathologic findings.