아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

Carotenoids 식이와 알코올이 혈액학적 성분과 간조직에 미치는 영향에 관한 연구

조만희,김연선,이상한,우기민,장예진,김창세 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Alcohol is well known agent which can damage the human tissues such as liver via stimulating lipid peroxidation and storage, denaturation of macromolecules, and inhibiting protein metabolism. On the other hand, carotenoids in addition to vitamins A, C, E and lipoic acid, play important roles in protecting these oxidative damages as well as preventing the production of free radicals. This study was carried out to elucidate the precise effects of alcohol administration into rats on the antioxidative functions of dietary carotenoids and isolated β-carotene, and to find out any parameters to uncover more detailed biochemical mechanisms of these agents. For these purposes, two different approaches were performed: 1) examination of the changes in hematological parameters (e.g., total proteins, A/G ratio, ALT/AST et. al) and statistical correlations among inter- and intragroups, 2) examination of the histopathological changes by an electron microscope. The results were analyzed and summarized as following; (1) Carrot diet for one week caused a slight increase in albumin. A/G ratio and AST levels, and a slight decrease in ALT, ALP, BUN and uric acid levels. Two-week alcohol administration following carrot diet increased total proteins, albumin A/G ratio and BUN levels. However, both groups were shown to have little significant changes in cholesterol concentrations. (2) Cheese diet for one week caused a significant increase in total proteins, albumin, AST, ALP, uric acid and total cholesterol levels, and a significant decrease in A/G ratio and glucose concentrations. However, two-week β-carotene diet following cheese intake increased total proteins albumin, A/G ratio, BUN and total cholesterol levels, whereas AST, ALT, ALP and uric acid levels were decreased. (3) Alcohol administration for one week caused an increase in AST activities and a decrease in total proteins, albumin, ALT, ALP and BUN levels. A successive β-carotene diet following alcohol administration increased total proteins, albumin, ALT, ALP and BUN levels, whereas decreased AST and uric acid levels. However, there were not significant changes in A/G ratio, glucose and total cholesterol levels in both groups. (4) β-carotene diet for one week caused a slight increase in albumin, glucose, AST and uric acid levels, and a slight decrease in ALP and BUN levels. Two-weeks alcohol administration following β-carotene diet increased albumin, glucose, BUN and total cholesterol levels, whereas decreased AST, ALT, ALP and uric acid levels. (5) Although the pathological investigation on the liver did not reveal significant changes, cheese diet group (CH-BC/1-CH) was shown to have some lipid deposits. Some results were unexpected and different from typical hematological changes shown by other researchers. Nonetheless, these results strongly suggest that the liver damage or hepatism caused by alcohol intake affects many kinds of biochemical metabolisms, which results in significant changes in many hematological parameters. In addition, dietary carotenoid and isolated β-carotene were shown to have protective roles against the biochemical changes by alcohol intake.

Projections of summertime ozone concentration over East Asia under multiple IPCC SRES emission scenarios

Lee, Jae-Bum,Cha, Jun-Seok,Hong, Sung-Chul,Choi, Jin-Young,Myoung, Ji-Su,Park, Rokjin J.,Woo, Jung-Hun,Ho, Changhoi,Han, Jin-Seok,Song, Chang-Keun Elsevier 2015 Atmospheric environment Vol.106 No.-

<P><B>Abstract</B></P> <P>We have developed the Integrated Climate and Air Quality Modeling System (ICAMS) through the one-way nesting of global–regional models to examine the changes in the surface ozone concentrations over East Asia under future climate scenarios. Model simulations have been conducted for the present period of 1996–2005 to evaluate the performance of ICAMS. The simulated surface ozone concentrations reproduced the observed monthly mean concentrations at sites in East Asia with high R<SUP>2</SUP> values (0.4–0.9), indicating a successful simulation to capture both spatial and temporal variability. We then performed several model simulations with the six IPCC SRES scenarios (A2, A1B, A1FI, A1T, B1, and B2) for the next three periods, 2016–2025 (the 2020s), 2046–2055 (the 2050s), and 2091–2100 (the 2090s). The model results show that the projected changes of the annual daily mean maximum eight-hour (DM8H) surface ozone concentrations in summertime for East Asia are in the range of 2–8 ppb, −3 to 8 ppb, and −7 to 9 ppb for the 2020s, the 2050s, and the 2090s, respectively, and are primarily determined based on the emission changes of NO<SUB>x</SUB> and NMVOC. The maximum increases in the annual DM8H surface ozone and high-ozone events occur in the 2020s for all scenarios except for A2, implying that the air quality over East Asia is likely to get worse in the near future period (the 2020s) than in the far future periods (the 2050s and the 2090s). The changes in the future environment based on IPCC SRES scenarios would also influence the change in the occurrences of high-concentrations events more greatly than that of the annual DM8H surface ozone concentrations. Sensitivity simulations show that the emissions increase is the key factor in determining future regional surface ozone concentrations in the case of a developing country, China, whereas a developed country, Japan would be influenced more greatly by effects of the regional climate change than the increase in emissions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Change in ozone level is determined by the combination of NO<SUB>x</SUB> and NMVOC emissions. </LI> <LI> Ozone level over East Asia would get worse in the near future (the 2020s) than far future. </LI> <LI> A high-concentration event generally increases and more frequently occurs in the future. </LI> <LI> Change in ozone air quality in a developing country depends more on emissions change. </LI> <LI> Change in ozone air quality in a developed country depends more on climate change. </LI> </UL> </P>

Thermoplasma acidophilum의 20S Proteasome에 의한 ATPase-활성적 단백질분해에 관한 연구

우기민,장예진,조만희,김창세,김완종,조성호,이상한 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

The eukaryotic 26S proteasome is an ATP/ubiquitin-dependent proteolytic complex consisting of the 20S core particle and 19S ATPase complex. However, because of its complexity and unstable properties, this study was carried out to present more simple and stable model for the ATP-activated proteolytic complex in prokaryotes which can take the place of the eukaryotic 26S proteasome. For this purpose, recombinant Thermoplasma 20S proteasome (T20S) and Methanococcus MS4, a sequence homolog of one ATPase subunit in the 19S ATPase complex, were successfully isolated from Escherichia coli (E. coli). The α and β subunits of T20S expressed in E. coli could assemble for themselves, and showed the peptide-hydrolyzing activity. Whereas both T20S and R20S (the 20S complex from rabbit skeletal muscle) had the highest peptidase activity against Suc-LLVY-AMC, a good substrate for chymotrypsin-like peptidase activity, the specific activity of T20S was slightly lower than that of R20S. In addition, several reagents such as KCI, SDS, and ovalbumin were shown to have different effects on the peptidase activities between T20S and R20S. When the ATPase activity of the purified MS4 were assayed , the Km for ATP was about 0.5 mM, and casein could stimulate the activity more than 2 fold without the change in Km. This result implicates that protein-activated ATPase may induce the conformational change of casein, and therefore suggests that MS4 ATPase may activate the proteolytic activity of the 20S proteasome via accelerating the recognition and translocation of the protein substrates.

혐기화 시간에 따른 활성슬러지의 물리·화학적 특성변화

이창한,나영수,김도한,이송우,송승구 한국환경과학회 2002 한국환경과학회지 Vol.11 No.4

Physico-chemical properties of the activated sludges(Suyoung and Changlim treatment plant). such as SVI(sludge volume index), absorbance, specific surface area, and specific resistance using Buchener funnel test were investigated with changing anaerobic storage time. This experimental condition was found that it was possible to estimate a linear relationship between their parameters such as specific area, specific resistance, and sludge volume index(SVI). The specific surface area and the specific resistance to filtration of the activated sludges of Suyoung and Changlim treatment plant were found as 123.6~136.6㎡/gDS and 41.5 ~ 44.9㎡/gDS(dry solid), and 1.09×10^14 ~ 5.48×10^14m/㎏, respectively. The results gave a good linear relationship between the specific surface area and the specific resistance. r=2.25×10^12s-8.10×10^13(R^2=0.8885) at Suyoung treatment plant and r=1.26×10^13s-4.75×10^14(R^2=0.8756) at Canglim treatment plact.

感染性 疾患의 臨床疫學的 硏究

張友鉉,朴熙明,金漢壽,李承薰 現代醫學社 1969 現代醫學 Vol.10 No.4

The advancement of modern medicine, particularly the application of effective chemotherapeutics and preventive measures, have brought significant changes on the ecological aspect of microbial diseases of man. It was thought worthwhile to study the recent trends and present status of microbial infections in this country. Thus, the relative incidences and their etiological relationships of bacterial species, the pattern of drug sensitivities among the strains, and also the cases diagnosed by the serological methods were studied for the patients of SNU Hospital from January 1963 to December 1967. The results might be summarized as follows; 1. Relationships between bacteria and diseases (1) The species and their relative incidences of bacteria, isolated from various abscess, were α-hemolytic streptococcus 26.6%, Staphyloccus aureus 22.6%, paracolon bacilli 9.9%, coliform bacilli 8.1%, Klebsiella pneumoniae 7.6%, E. coil 6.9%, β-hemolytic streptococcus 5.9%. Staphylococcus albus 3.8%. Proteus 3.8%. Alkaligenes fecalis 2.1%, Diplococcus pneumoniae 1.8% and Pseudomonas aeruginosa 1.6%. (2) The species and their relative incidences of bacteria, isolated from the pyelonephritis cases, were E. coli, 39.5%, coliform bacilli 13.5%, Pseudomonas aeruginosa 9.3%, Proteus 7.4%, Staphylococcus albus 4.6%, α-hemolytic streptococcus 4.6%, Staphylococcus aureus 2.8%, Klebsiella pnemoniae 2.8%, and Alkaligenes fecalis 2.8%. (3) The species and their relative incidences of bacteria, isolated from the osteomyelitis cases, were Staphylococcus aureus 46.5%, β-hemolytic streptococcus 12%, Staphyococcus albus 12%, coliform bacilli 6.9%, α-hemolytic streptococcus 6.9%, paracolon bacilli 5.2% and E. coli 3.5%. (4) The species and their relative incidences of bacteria, isolated from the surgical infections were Staphylococcus aureus 33%, E. coli 15.1%, coliform bacilli 11.6%, Pseudomonas aeruginosa 10.7%, paracolon bacilli 9.8%, Proteus 7.1%, α-hemolytic streptococcus 3.5%, Klebsiella pneumoniae 2.7%, and Alkaligenes fecalis 0.9%. (5) The species and their relative incidences of bacteria, isolated from the pneumonia cases, were Diplococcus pneumoniae 14.4%, Klebsiella pneumoniae 9.6%, Staphylococcus aureus 10.3%, β-hemolytic streptococcus 4.8%, paracolon bacilli 4.8%, E. coli 3.4%, coliform bacilli 3.4%, α-hemolytic streptococcus 34.6% and Staphylococcus albus 13.6%. (6) 44 strains of Salmonella group D were isolated. (7) 207 cases of Salmonella typhosa infections, 39 cases of Salmonella paratyphi A infer ctions and 20 cases of Salmonella paratyphi B infections were diagnosed by Widal. Reactions. (8) No significant change in the yearly pattern between the bacterial species isolated and clinical cases was noticed. 2. Drug-sensitivities of the bacterial strains. (1) Percentage of Staphylococcus aureus strains sensitive to streptomycin, chloramphenicol. Or terramycin appeared to have fluctuated by year. Percentages of the strains sensitive to penicillin, erythromycin albamycin, and kanamycin were 25%, 85%, 99% and 99% respectively, without significant change by year. (2) Perentage of E. coil strain's sensitive to streptomycin, erythromycin, chloramphenicol albamycin and kanamycin were 30%, 9% 70%, and 70% respectively, without significant change by year. Percentage of the strains sensitive to terramjcin decreased from 33.3% in 1963 to 11.6% in 1967 to kanamycin increased from 72% in 1963 to 82% in 1967. None of the strains was sensitive to penicillin. (3) Percentage of proteus strains sensitive to penicillin, terramycin, streptomycin and chloramphenicol were 5%, 3%, 36% and 39%, respectively, without significant change by year. Percentage of the strains sensitive to albamycin decreased from 72.5% in 1963 to 50% in 1967 and to kaiiainycin increased from 72% in 1963 to 82% in 1967. None of the strain was sensitive to erythromycin. (4) Percentage of Pseudomonas strains sensitive to penicillin, streptomycin chloramphenicol, terramycin, erythromycin, albamycin and kanamycin were wider 20% (5) Percentage of coliform bacilli strains sensitive to penicillin, streptomycin, chloramn henicol, terramycin, erythromycin and albamycin were 10%, 24%, 28%, 20% and 10% respectively, without significant change by year. Percentage of the strains sensitive to albamycin appeared to have fluctuated by year. The strains sensitive to kanamycin decreased from 80% in 1963 to 56% in 1967. (6) Pereeentage of paracolon bacilli sensitive to penicillin, streptomycin, chloramphenicol, terrahiycin, erythromycin, kanamycin and albamycin were 3%, 27%, 33%, 20%, 8%, 80% and 65% respectively, without significant change by year.

안전상비의약품 판매 이후 중독환자 특성 변화

김창영,이의중,이성우,김수진,한갑수,Kim, Chang Yeong,Lee, Eui Jung,Lee, Sung Woo,Kim, Su Jin,Han, Kap Su 대한임상독성학회 2018 대한임상독성학회지 Vol.16 No.1

Purpose: On November 15, 2012, sales of OTC (Over-The-Counter) drugs began at convenience stores, which changed the accessibility of some drugs. As a result, the exposure and access patterns of these drugs could have changed. In this study, we reviewed the changes in the characteristics of drug poisoning patients because of the reposition of nonprescription drugs according to the revised Pharmaceutical Affairs Act. Methods: A retrospective study was conducted to evaluate changes in characteristics of drug poisoning patients between 2008 and 2016. A registry was developed by an emergency medical center in a local tertiary teaching hospital, and patients who visited the center were enrolled in this registry. We compared two periods, from 2008 to 2012 (Pre OTC) and from 2013 to 2016 (Post OTC), for type of intoxicant, time from poisoning to visiting the emergency center, intention, psychiatric history, previous suicidal attempt, alcohol status, and emergency room outcomes. The primary outcome was the number of patients who took acetaminophen and NSAIDs (nonsteroidal anti-inflammatory drugs). Secondary outcomes were ICU admission rate, mortality rate, and number of patients who visited the ER when the pharmacy was closed after taking acetaminophen and NSAIDs (nonsteroidal anti-inflammatory drugs). Results: Among 1,564 patients, 945 and 619 patients visited the emergency room during pre and post OTC periods. The number of patients with acetaminophen and NSAIDs poisoning decreased from 9.2% to 6.1% (p=0.016). The ICU admission rate and mortality rate in the emergency room did not show significant results in the relevant patient groups, and so was the number of patients visiting ER when the pharmacy was closed taking acetaminophen and NSAIDs. Conclusion: Despite the sales of nonprescription drugs at convenience stores, the number of acetaminophen and NSAIDs poisoning patients decreased.

월경전 기분 변화와 출산후 불안-우울 증상간의 상관성

한창수,김용구,이낙우,김 탁,김해중,김 현,조숙행 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.1

To investigate the relationship between premenstrual mood changes and anxiety, and mood symptoms in the perinatal period , questionaire survey was carried out. There was no significant correlation between premenstrual symptom scale value and Edinburgh postpartum depression scale value. And theme was no relationship between premenstrual symptom scale value and state anxiety inventory scale value in the perinatal period. This findings suggest that premenstrual mood changes are not directly correlated with mood changes in the perinatal period.

국내 한의학 학술지에 발표된 동의보감 연구 현황 조사

한창현(Chang-hyun Han),박상영(Sang-young Park),권오민(Oh-min Kwon),안상영(Sang-young Ahn),안상우(Sang-woo Ahn) 한국의사학회 2009 한국의사학회지 Vol.22 No.2

Background : To understand Korean medicine it is crucial to first understand medical aspect of ‘DongUiBoGam’. It is also meaningful that it became one of the influential book even to posterity. Also based on the apprehension, we can take a view of development of Korean medicine led by ‘DongUiBoGam’. Objectives : This study aims to review the status, study field, specialist of DongUiBoGam. In the process, this review will grasp trends in this field of studies and will direct further researches into the right direction. Method : The computerized Korean databases were searched from their respective inceptions up to December 2008. The search terms used were ‘DongUiBoGam’ and random or Korean language terms related to DongUiBoGam. Several specialized journals were also manually searched for relevant articles. Result : Since the 2000s, DongUiBoGam papers in the Korean Literature is increased. Published 58 papers on The Korean Journal of Oriental Medical Prescription were more than the other journals. 58 papers published in The Korean Journal of Oriental Medical Prescription are the best in many areas related to Korean medicine. Most people have submitted papers related to DongUiBoGam was Woo-yeal Jeong. Conclusions : ‘DongUiBoGam’ research is conducted and current tendency and outlook for ‘DongUiBoGam’ is carried out focusing on several associations.

토끼에서 신델라 겔 ( 송아지의 제단백혈액추출물 황산 미크로노아이신 = 20 1 혼합물 ) 의 피부자극성시험

남석우(Suk Woo Nam),이영진(Young Jin Lee),고영권(Young Kwon Ko),장만식(Man Sik Chang),최완수(Wahn Soo Choi),김규봉(Kyu Bong Kim),우태욱(Tae Wook Woo),한정환(Jeung Whan Han),홍성렬(Sung Youl Hong),이향우(Hyang Woo Lee) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.4

This study was conducted to investigate the skin irritation toxicity of Syndella gel, a combination topical drug containing a deproteinised dialysate of calfs blood and micronomicin sulfate in the ratio of 20 to 1, in New Zealand White rabbits. In the primary skin irritation test with male New Zealand White rabbits, there was no treatment-related effect on clinical sign, and body weight was not significantly changed. The Primary Irritation Index (PII) was 0.33, indicating that Syndella gel was a mildly irritating formulation.