Prevalence of Anti-deamidated Gliadin Peptide Antibodies in Asian Patients With Irritable Bowel Syndrome

( Wei Lu ),( Kok Ann Gwee ),( Kewin Tien Ho Siah ),( Jin Yong Kang ),( Ru Min Lee ),( Cecilia Cheng Lai Ngan ) 대한소화기기능성질환·운동학회 2014 Journal of Neurogastroenterology and Motility (JNM Vol.20 No.2

Background/AimsNon-celiac gluten sensitivity has been increasingly recognized as a predisposing factor for irritable bowel syndrome (IBS)-likesymptoms in Western populations where celiac disease (CD) is relatively common. In Asia where CD is rare, we wish to determinethe prevalence of gluten protein associated serology in IBS patients, which has not been formally studied, and its relationto histological and human leukocyte antigen (HLA) markers. MethodsWe reviewed a consecutive cohort of Asian patients with IBS, who had undergone serologic testing for IgA against deamidatedgliadin peptide antibodies (IgA DGP) and IgA anti-endomysium antibodies, and who also had duodenal biopsies during clinicalworkup. In addition, a subset of Chinese patients with positive serology was further tested for HLA-DQ2 and HLA-DQ8. ResultsOf 186 patients, 34 (18%) were positive for IgA DGP; bloating, abdominal pain, belching and diarrhea were the most com -monly reported symptoms but diarrhea as the most bothersome symptom was significantly more common in IgA DGP positivepatients. Mildly increased intra-epithelial lymphocytes on duodenal biopsy was also more common (29% vs. 9%, P = 0.001). Nine of 21 Chinese patients tested as IgA DGP positive undertook HLA-DQ2/DQ8 testing, with only 2 being positive forHLA-DQ8. All patients with positive IgA DGP reported symptom improvement with gluten withdrawal. ConclusionsWe have described a series of Asian, mainly Chinese, patients with IBS who were tested positive for IgA DGP, and improvedon a gluten exclusion diet. We believe this is the first report of non-celiac gluten sensitivity in Asia, a region where CD isuncommon

Expression and Characterization of Protein Latcripin-3, an Antioxidant and Antitumor Molecule from Lentinula edodes C91-3

Ann, Xiao-Hua,Lun, Yong-Zhi,Zhang, Wei,Liu, Ben,Li, Xing-Yun,Zhong, Min-Tao,Wang, Xiao-Li,Cao, Jing,Ning, An-Hong,Huang, Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

In this study, an anti-oxidant and anti-tumor protein Latcripin-3 of Lentinula edodes C91-3 was expressed in Escherichia coli. for the first time. According to the cDNA library, the full-length gene of Latcripin-3 was cloned by the methods of 3'-full rapid amplification of cDNA Ends (RACE) and 5'-full RACE. The structural domain gene of Latcripin-3 was inserted into the pET32 a(+). The functional protein of Latcripin-3 was expressed in Rosetta-gami (DE3) E. coli, evaluated by Western blotting and mass spectrometry. DPPH testing showed that the protein Latcripin-3 can scavenge free radicals remarkably well. The activity of functional protein Latcripin-3 on A549 cells was studied with flow cytometry and the MTT method. The MTT assay results showed that there was a decreases in cell viability in a dose-dependent and time-dependent manner in protein Latcripin-3 treated groups. Flow cytometry demonstrated that Latcripin-3 can induce apoptosis and block S phase dramatically in human A549 lung cancer cells as compared to the control group. At the same time, the cell ultrastructure observed by transmission electron microscopy supported the results of flow cytometry. This research offers new insights and advantages for identifying anti-oxidant and anti-tumor proteins.

Retrospective Molecular Epidemiology Study of PD-L1 Expression in Patients with EGFR-Mutant Non-small Cell Lung Cancer

조종호,Wei Zhou,최윤라,선종무,최혜주,김태은,Marisa Dolled-Filhart,Kenneth Emancipator,Mary Anne Rutkowski,김진국 대한암학회 2018 Cancer Research and Treatment Vol.50 No.1

Purpose Data are limited on programmed death ligand 1 (PD-L1) expression in epidermal growth factor receptor (EGFR)-mutant non-small cell lung cancer (NSCLC). Materials and Methods We retrospectively evaluated the relationship between PD-L1 expression and recurrencefree survival (RFS) and overall survival in 319 patients with EGFR-mutant NSCLC who were treated at Samsung Medical Center from 2006 to 2014. Membranous PD-L1 expression on tumor cells was measured using the PD-L1 IHC 22C3 pharmDx antibody and reported as tumor proportion score (TPS). Kaplan-Meier methods, log-rank test, and Cox proportional hazards models were used for survival analysis. Results All patients had ! 1 EGFR mutation—54% in exon 19 and 39% in exon 21. Overall, 51% of patients had PD-L1–positive tumors. The prevalence of PD-L1 positivity was higher among patients with stages II-IV versus stage I disease (64% vs. 44%) and among patients with other EGFR mutations (75%) than with L858R mutation (39%) or exon 19 deletion (52%). PD-L1 positivity was associated with shorter RFS, with an adjusted hazard ratio of 1.52 (95% confidence interval [CI], 0.81 to 2.84; median, 18 months) for the PD-L1 TPS ! 50% group, 1.51 (95% CI, 1.02 to 2.21; median, 31 months) for the PD-L1 TPS 1%-49% group, and 1.51 (95% CI, 1.05 to 2.18) for the combined PD-L1–positive groups (TPS ! 1%) compared with the PD-L1–negative group (median, 35 months). Conclusion PD-L1 expression is associated with disease stage and type of EGFR mutation. PD-L1 positivity might be associated with worse RFS among patients with surgically treated EGFRmutant NSCLC.

Discovery of 2-(3,5-difluoro-4-methylsulfonaminophenyl)propanamides as potent TRPV1 antagonists

Kim, Changhoon,Ann, Jihyae,Lee, Sunho,Sun, Wei,Blumberg, Peter M.,Frank-Foltyn, Robert,Bahrenberg, Gregor,Stockhausen, Hannelore,Christoph, Thomas,Lee, Jeewoo Elsevier 2018 Bioorganic & medicinal chemistry letters Vol.28 No.14

<P><B>Abstract</B></P> <P>A series of A-region analogues of 2-(3-fluoro-4-methylsufonamidophenyl) propanamide <B>1</B> were investigated as TRPV1 antagonists. The analysis of structure-activity relationship indicated that a fluoro group at the 3- (or/and) 5-position and a methylsulfonamido group at the 4-position were optimal for antagonism of TRPV1 activation by capsaicin. The most potent antagonist <B>6</B> not only exhibited potent antagonism of activation of <I>h</I>TRPV1 by capsaicin, low pH and elevated temperature but also displayed highly potent antagonism of activation of <I>r</I>TRPV1 by capsaicin. Further studies demonstrated that antagonist <B>6</B> blocked the hypothermic effect of capsaicin <I>in vivo</I>, consistent with its <I>in vitro</I> mechanism, and it showed promising analgesic activity in the formalin animal model.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A series of A-region analogues of 2-(3-fluoro-4-methylsufonamidophenyl)propanamide were investigated as TRPV1 antagonists. </LI> <LI> Compound <B>6</B> showed highly potent antagonism toward capsaicin activation. </LI> <LI> Compound <B>6</B> displayed anti-hypothermic effect and promising analgesic activity <I>in vivo</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

The accuracy and clinical applicability of a sensor based electromagnetic nonfluoroscopic catheter tracking system

Shinya Yamada,Li-Wei Lo,Yenn-Jiang Lin,Shih-Lin Chang,Fa-Po Chung,Yu-Feng Hu,Ta-Chuan Tuan,Tze-Fan Chao,Jo-Nan Liao,Chin-Yu Lin,Shih-Ann Chen 대한심장학회 2019 Korean Circulation Journal Vol.49 No.1

Background and Objectives: The differences between electromagnetic-based mapping (EM) and impedance-based mapping (IM) in 3D anatomical reconstruction have not been fully clarified. We aimed to investigate the anatomical accuracy between EM (MediGuide™) and IM (EnSite Velocity™) systems. Methods: We investigated 15 consecutive patients (10 males, mean age 58±9 years) who underwent pulmonary veins (PVs) isolation for paroxysmal atrial fibrillation (PAF). Contrast-enhanced computed tomography (CT) image of the left atrium (LA) was acquired before ablation and the 3D geometry of the LA was constructed using EM during ablation procedure. We measured the 4 PV angles between the main trunk of each PV and the posterior LA after field scaling. Additionally, the posterior LA surface area was measured. The variables were compared to those of CT-based geometry. A control group of 40 patients who underwent conventional PVs isolation using IM were also evaluated. Results: The actual and relative changes of EM and CT-based geometry in all PV angles and posterior LA were significantly smaller compared to those of IM and CT-based geometry. Intraclass correlation coefficient (ICC) between EM and CT-based geometry were 0.871 (right superior pulmonary vein [RSPV]), 0.887 (right inferior pulmonary vein [RIPV]), 0.853 (left superior pulmonary vein [LSPV]), 0.911 (left inferior pulmonary vein [LIPV]), and 0.833 (posterior LA). On the other hand, ICC between IM and CT-based geometry were 0.548 (RSPV), 0.639 (RIPV), 0.691 (LSPV), 0.706 (LIPV), and 0.568 (posterior LA). Conclusions: Image integration with EM enables high accurate visualization of cardiac anatomy compared to IM in PAF ablation.

A Model-Based Analysis of Secure Video Transmission Based on IPSec and IPv4

Quang-Dao Van,Anne Wei,Benoît Geller,Gérard Dupeyrat 한국전자통신연구원 2005 ETRI Journal Vol.27 No.2

A promising solution to protect wired Internet networks is to use the Secure Internet Protocol (IPSec); however, this has some drawbacks, particularly on the quality of service (QoS). This paper aims at evaluating the video traffic QoS in terms of end-to-end delay and packet loss rate. Based on some basic assumptions, our analysis shows that the performance with IPSec is rapidly inferior to the IPv4 performance. We thus suggest adding some QoS parameters into IPSec in order to achieve a compromise between QoS and security.

A Derivative of Chrysin Suppresses Two-Stage Skin Carcinogenesis by Inhibiting Mitogen- and Stress-Activated Kinase 1

Liu, Haidan,Hwang, Joonsung,Li, Wei,Choi, Tae Woong,Liu, Kangdong,Huang, Zunnan,Jang, Jae-Hyuk,Thimmegowda, N.R.,Lee, Ki Won,Ryoo, In-Ja,Ahn, Jong-Seog,Bode, Ann M.,Zhou, Xinmin,Yang, Yifeng,Erikson, American Association for Cancer Research 2014 CANCER PREVENTION RESEARCH Vol.7 No.1

<P>Mitogen- and stress-activated kinase 1 (MSK1) is a nuclear serine/threonine protein kinase that acts downstream of both extracellular signal-regulated kinases and p38 mitogen-activated protein kinase in response to stress or mitogenic extracellular stimuli. Increasing evidence has shown that MSK1 is closely associated with malignant transformation and cancer development. MSK1 should be an effective target for cancer chemoprevention and chemotherapy. However, very few MSK1 inhibitors, especially natural compounds, have been reported. We used virtual screening of a natural products database and the active conformation of the C-terminal kinase domain of MSK1 (PDB id 3KN) as the receptor structure to identify chrysin and its derivative, compound 69407, as inhibitors of MSK1. Compared with chrysin, compound 69407 more strongly inhibited proliferation and 12-<I>O</I>-tetradecanoylphorbol-13-acetate (TPA)-induced neoplastic transformation of JB6 P+ cells with lower cytotoxicity. Western blot data demonstrated that compound 69407 suppressed phosphorylation of the MSK1 downstream effector histone H3 in intact cells. Knocking down the expression of MSK1 effectively reduced the sensitivity of JB6 P+ cells to compound 69407. Moreover, topical treatment with compound 69407 before TPA application significantly reduced papilloma development in terms of number and size in a two-stage mouse skin carcinogenesis model. The reduction in papilloma development was accompanied by the inhibition of histone H3 phosphorylation at Ser10 in tumors extracted from mouse skin. The results indicated that compound 69407 exerts inhibitory effects on skin tumorigenesis by directly binding with MSK1 and attenuates the MSK1/histone H3 signaling pathway, which makes it an ideal chemopreventive agent against skin cancer. <I>Cancer Prev Res; 7(1); 74–85. ©2013 AACR</I>.</P>

Genistein Decreases food Intake, Body Weight, and Fat Pad Weight and Causes Adipose Tissue Apoptosis in Ovariectomized Female Mice

Hye-Kyeong Kim,Cassandra Nelson-Dooley,Mary Anne Della-Fera,Jeong-Yeh Yang,Wei Zhang.y Jiuhua Duan,Diane L. Hartzell,Mark W. Hamrick,Clifton A. Baile,성소희 전주국제발효식품엑스포 조직위원회 2006 발효 : 맛있는 잡지 Vol.2 No.2

Influence of the River Ceasing on Wetland Environment in the Yellow River Delta

( Yan Xi Shi ),( Seong Won Ann ),( Wei Feng Chen ),( Su Feng Tian ),( Hong Nam Kim ) 한국녹지환경디자인학회 2005 녹지환경학회지 Vol.1 No.1

The Yellow River came to cease, which was affected by natural factors and the unreasonable human activities. The flow broke in the Yellow River, and water and sediment flowing into the sea decreased, which lowered the speed of newly formed wetland extending to the sea. The water environment deteriorated; its composing structure tended to be unsteady; the biologic diversity decreased and wetland function reduced. To ensure that the Yellow River delta and its ecosystem develops sustainability, it is significant to reduce times and days of the ceasing, keep certain runoff and sediments in the river to the sea and make its watercourse stable.

Spontaneous, Defect-Free Kinking via Capillary Instability during Vapor–Liquid–Solid Nanowire Growth

Li, Yanying,Wang, Yanming,Ryu, Seunghwa,Marshall, Ann F.,Cai, Wei,McIntyre, Paul C. American Chemical Society 2016 Nano letters Vol.16 No.3

<P>Kinking, a common anomaly in nanowire (NW) vapor-liquid-solid (VLS) growth, represents a sudden change of the wire's axial growth orientation. This study focuses on defect-free kinking during germanium NW VLS growth, after nucleation on a Ge (111) single crystal substrate, using Au-Ge catalyst liquid droplets of defined size. Statistical analysis of the fraction of kinked NWs reveals the dependence of kinking probability on the wire diameter and the growth temperature. The morphologies of kinked Ge NWs studied by electron microscopy show two distinct, defect-free, kinking modes, whose underlying mechanisms are explained with the help of 3D multiphase field simulations. Type I kinking, in which the growth axis changes from vertical [111] to < 110 >, was observed in Ge NWs with a nominal diameter of similar to 20 nm. This size coincides with a critical diameter at which a spontaneous transition from < 111 > to < 110 > growth occurs in the phase field simulations. Larger diameter NWs only exhibit Type II kinking, in which the growth axis changes from vertical [111] directly to an inclined < 111 > axis during the initial stages of wire growth. This is caused by an error in sidewall facet development, which produces a shrinkage in the area of the (111) growth facet with increasing NW length, causing an instability of the Au-Ge liquid droplet at the tip of the NW.</P>