상황버섯 추출물의 항산화 및 성분분석

김아름 ( A Reum Kim ),김정은 ( Jung Eun Kim ),박수남 ( Soo Nam Park ) 대한화장품학회 2011 대한화장품학회지 Vol.37 No.4

본 연구에서는 상황버섯 추출물의 항산화 및 항노화 활성 및 항균 효과, 그리고 성분분석에 관한 연구를 수행하였다. 상황버섯 추출물의 자유 라디칼(1,1-diphenyl-2- picrylhydrazyl, DPPH) 소거활성(FSC₅₀)은 에틸아세테이트(ethyl acetate) 분획(2.94 μg/mL)에서 가장 큰 활성을 나타내었고, 루미놀-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 상황버섯 추출물의 총항산화능은 추출물의 에틸아세테이트 분획(0.0072 μg/mL)에서 가장 큰 활성을 나타내었다. 광증감제인 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 억제 효과를 측정하였을 때 농도범위(5 ~ 50 μg/mL)에서 50 % 에탄올 추출물과 에틸아세테이트 분획 모두 농도·의존적으로 세포 보호 효과를 나타내었다. 타이로시네이즈의 활성 저해 효과(IC₅₀)를 측정한 결과 50 % 에탄올 추출물(IC₅₀ = 6.34 μg/mL)에서 우수한 효과를 나타내었으며, 엘라스테이즈의 활성 저해 효과(IC₅₀)는 에틸아세테이트 분획(IC₅₀ = 14.08 μg/mL)에서 큰 효과가 나타났다. TLC, HPLC 및 LC/ESI-MS를 이용하여 상황버섯 추출물 ethyl acetate 분획의 주성분을 분석하였고 hispidin 유도체인 interfungin A를 확인하였다. 이상의 결과들은 상황버섯 추출물이 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로써 작용할 수 있으며, 특히 상황버섯 추출물의 에틸아세테이트 분획을 항산화, 항노화 및 미백 기능성 화장품 소재로써의 응용 가능성을 확인하였다. In this study, the antioxidative effect, antibacterial, inhibitory effects on tyrosinase, inhibitory effects on elastase and component analysis of Phellinus linteus (P. linteus) extracts were investigated. The ethyl acetate fraction of P. linteus extracts (2.94 μg/mL) showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC₅₀). Reactive oxygen species (ROS) scavenging activity (OSC₅₀) of P. linteus extracts on ROS generated in Fe³⁺-EDTA/H₂O₂ system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction (0.0072 μg/mL) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of P. linteus extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The P. linteus extracts showed cellular membrane protective effects in a concentration dependent manner (5 ~ 50 μg/mL). The inhibitory effect (IC₅₀) on tyrosinase of P. linteus extract was the highest at 50 % ethanol extract (6.34 μg/mL), and the inhibitory effect (IC₅₀) on elastase of P. linteus was the highest at ethyl acetate fraction (14.08 μg/mL). TLC, HPLC chromatogram and LC/ESI-MS of the ethyl acetate fraction obtained from P. linteus extracts were identified interfungin A (PL RPT-1a). These results indicate that extract/fractions of P. linteus can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging ROS, and protect cellular membranes against ROS. Extract/fractions of P. linteus can be applicable to new cosmeceuticals for antioxidant, antiaging, antiwrinkle and whitening.

The Role of Plant Extracts in Alleviating Particulate Matter-induced Inflammation in Barrier-interrupted Skin

( Ji Yeon Hong ),( Sun Young Choi ),( Jae Wan Park ),( Jin O Park ),( A Reum Kim ),( Beom Joon Kim ) 대한피부과학회 2021 大韓皮膚科學會誌 Vol.59 No.3

Background: The adverse effects of particulate matter (PM) on the skin have been concerning recently. Objective: We investigated PM induced inflammation on barrier-interrupted skin and relieving effects of plant extracts on PM-induced skin irritation. Methods: A total of 20 participants were enrolled for the induction of skin irritation. Sodium lauryl sulfate (SLS) patch was affixed to the forearm for 24 hours. After patch removal, total suspended particulates (TSPs) were applied. After inducing skin irritation using SLS and TSP, cream containing 0.1% mixture of plant extracts (Citrus sunki, Sceptridium ternatum (Botrychium ternatum), and Korthalsella japonica) was applied. The a* value, erythema index (EI), and transepidermal water loss (TEWL) were measured. Results: The a* value was significantly higher at the site where TSPs were applied than that at the site where TSPs were not applied at 1 and 2 days after SLS patch removal. The a* value, EI, and TEWL were significantly low in the cream-applied area 7 days after the introduction of the SLS patch and TSPs. Conclusion: PM induced skin irritation in a previously disrupted skin barrier. Topical application of plant extracts alleviated skin irritation symptoms caused by dust exposure. (Korean J Dermatol 2021;59(3):217∼224)

급성 복통을 주소로 진단된 자발성 양측 부신 출혈에 의한 부신피질부전

조아름,홍주영,김동호,박민지,권미혜,박근용,원희관,임동미 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.S

Thomas Addison은 1855년 발표된 그의 논문에 일차성 부신피질부전에 의하여 발생한 근력 약화, 피로, 식욕부진, 복통, 체중감소, 색소침착 등에 대하여 처음으로 기술하였다[1,2]. 부신피질부전은 부신피질의 파괴에 의해 발생하는 당질 코르티코이드 결핍과 관련된 일차성과 뇌하수체에서 혈청 부신피질자극 호르몬(adrenocorticotropic hormone, ACTH)의 분비장애에 의해 발생하는 이차성으로 구분할 수 있다. 일차성과 이차성의 중요한 차이는 무기질 코르티코이드의 결핍이 일차성에는 있고 이차성에 는 없다는 점이다. 일차성 부신피질부전의 원인으로 이전에는 결핵에 의한 양측 부신 파괴가 가장 흔한 원인이었으나, 결핵 진단율 및 치료약제 보급률이 좋아진 20세기 후반부터는 감소 추세를 보이고 있으며, 선진국에서는 자가면역성 부신염에 의한 일차성 부신피질 부전이 80~90%로 가장 흔한 것으로 보고되었다[3]. 이외에 다른 감염성 질환, 전이성 암, 림프종, 부신출혈 또는 경색, 약물 등의 원인을 들 수 있다. 일차성 부신피질부전의 임상 증상과 징후로는 전신무력감, 식욕부진, 피로감, 체중감소, 과다한 색소침착, 오심, 복통, 기립성 저혈압, 저혈당, 전해질 장애 등이 올 수 있고, 심하면 탈수, 저혈압, 의식장애 및 쇼크가 동반될 수 있다. 일차성 부신피질부전은 본 증례와 같이 드물게 양측 부신 출혈성 경색으로 인해 발생되는 경우가 보고되었는데, 위험인자로는 항응고제 또는 헤파린 투여, 혈전성 질환, 항인지질 증후군과 같은 과응고 상태, 물리적 외상, 감염, 심한 스트레스와 관련되어 발생할 수 있으며 드물게 임신, 부신 종양, 쇼크 등이 원인이 되고 있다[4,5]. 그러나 본 증례와 같이 원인 없이 발생한 자발성 출혈에 의한 부신피질부전의 보고는 매우 드물다. 양측 부신 출혈의 임상 증상과 징후로는 저혈압, 쇼크, 복부, 등, 옆구리, 흉부의 통증, 발열, 의식저하, 복부 강직, 반발 압통 등이 있다[6]. 적절한 치료를 시행하지 않으면 쇼크가 진행하여 혼수와 사망에 이를 수 있어 정확하고 빠른 진단과 치료가 필요하다. 환자가 생존한다면 부신 기능은 드물게 수개월 후 정상으로 회복될 수 있다[7]. 저자들은 상복부 통증을 주소로 내원한 환자에서 매우 드물게 원인 없이 발생한 자발성 양측 부신 출혈에 의한 급성 부신피질부전 1예를 경험하였기에 보고하는 바이다.

조리 및 가공식품의 나트륨 함량

박영숙,이아름 순천향대학교 기초과학연구소 2011 순천향자연과학연구 논문집 Vol.17 No.2

This study was performed to know sodium levels of processed foods including fast foods, pouched foods, instant ramens, kimchi and soypaste products and snacks. We found that sodium levels of 10 different hamburgers averaged as 1.22~0.51g/each (3.1~1.3g salt) and 20 pizzas averaged as 1.69~0.32g/portion (4.3~0.8g salt). And pouched foods showed a wide range of sodium levels as 1300~790mg/pack (portion, 3.3~2.0g salt), instant ramens as 3000~1460mg/pack (portion, 7.6~3.7g salt) and snacks as 740~90mg/pack (portion, 1.9~0.2g). Sodium levels of kimchi and soypaste products in markets averaged as 1~2% and 6~8% respectively.

유기농업자재 원료 황련 추출물 중 Berberine, Coptisine 및 Palmatine 분석법 개발

문보연 ( Bo-yeon Moon ),송아름 ( A-reum Song ),류송희 ( Song-hee Ryu ),이효섭 ( Hyo-sub Lee ),박상원 ( Sang-won Park ),최근형 ( Geun-hyoung Choi ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-

최근에 식물추출물을 이용하는 병해충관리용 유기농업자재의 생산이 증가하면서 유기농업자재의 제조 개발이 활발하게 이루어지고 있다. 황련(Coptis)은 항균 효과, 제초 효과, 고추역병 방제, 배추좀나방 방제, 오이횐가루병 등 다양한 살충, 살균 효과가 있어 유기농업자재의 원료로 사용되고 있다. 이에 따라 황련 추출물로 이루어진 유기농업자재의 품질관리 및 효과적 사용을 위해 유효성분 확인 및 함량 분석법 개발에 대한 농업인, 농자재 생산사의 요구가 계속되고 있어 주성분 설정 및 분석법 개발이 필요하다. 본 시험에서는 황련의 주성분을 berberine, coptisine, palmatine으로 설정하고 정량분석을 위한 전처리법을 확립하고 제품 중 주성분의 함량을 분석하였다. 시료는 한 약재 황련을 주정과 ethanol로 추출하여 농축한 추출과 황련 추출물을 함유한 병해충관리용 유기 농업자재 시판제품 4종을 대상으로 하였다. 주성분의 함량을 HLB cartridge 60mg 3cc를 이용하여 정제하여 syringe filter(0.45um, PTFE)를 이용하여 거른 후 HPLC-UVD로 분석하였다. Column은 Luna 5u C18 (250*4.60mm 5micron), UV검줄파장은 346nm, 이동상은 용매A 0.1% Acetic acid in DW, 용매B 0.1% Acetic acid in ACN을 사용하여 gradient system으로 분석하였다. 그 결과 berberine, coptisine, palmatine의 표준품 검량선은 0.2~10 mg kg^-1 범위에서 0.999이상의 직선성 (R2)을 보였다. Coptisine의 회수율은 89.7~102.1%, Palmatine 87.1-94.1%, Berberine 86.3~97.5% 로 확인할 수 있었다. 황련의 EtOH 추출액의 함량은 3개 주성분의 합으로 3.6~8.95%가 나왔고, 황련이 포함된 유기농업자재 제품의 함량 분석 결과는 제품 A~D에서는 0.037~0.582%이었다. 이 실험에서 확립된 분석법은 황련 추출물과 황련 함유 유기농업자재 중 berberine, coptisine, palmatine의 함량분석에 활용할 수 있을 것으로 기대된다.

국내산 아로니아 두 품종(네로, 바이킹)의 영양성분 및 생리활성 분석

정연정,이아름,박재호,김영호,권의석,홍의연,한남수,엄현주,Jeong, Yeon-Jeong,Lee, A Reum,Park, Jae-Ho,Kim, Youngho,Kwon, Yeuseok,Hong, Eui Yon,Han, Nam Soo,Eom, Hyun-Ju 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.2

본 연구에서는 국내 충북에서 재배되는 아로니아의 활용성 제고를 위해 두 품종(네로, 바이킹)의 영양성분과 생리활성을 분석하였다. 수분 함량, 조회분, 조지방, 조단백, 조섬유, 탄수화물의 함량을 분석한 결과, 네로, 바이킹 각각 수분 함량은 13.21%, 13.94%, 조지방은 0.73%, 0.33%, 조단백 5.47%, 6.51%, 조섬유 15.65%, 16.15%, 조회분 0.58%, 0.64%였으며, 탄수화물은 100에서 위의 값을 뺀 값으로 64.36%, 62.43%로 검출되었다. 네로, 바이킹의 생리활성은 안토시아닌이 각각 233.23 mg%, 235.24 mg%이었으며, 총 폴리페놀 함량은 393.73 mg%, 510.60 mg%, DPPH 전자공여능은 71.31%, 74.48%, ${\alpha}$-glucosidase 저해활성은 77.93%, 88.64%로 측정되었다. 결과적으로 아로니아는 생리기능성 물질인 폴리페놀 화합물과 안토시아닌의 함량 및 항당뇨 활성이 높은 것으로 나타났다. 충북에서 재배된 아로니아의 두 품종인 네로와 바이킹을 비교하였을 때, 총 폴리페놀과 혈당강하능 면에서 네로보다 바이킹이 우수한 것으로 보아, 이를 바탕으로 바이킹을 이용한 다양한 가공품이 개발된다면 아로니아의 이용성이 증진될 것이라고 생각된다. The nutritional compositions and physicochemical properties of two aronia varieties (Viking and Nero) cultivated in Chungbuk were investigated. The proximate compositions of Nero and Viking powder were:13% moisture, 0.58% and 0.6% ash, 0.73% and 0.33% crude lipid, 5.47% and 6.51% crude protein, 15.65% and 16.15% crude dietary fiber, and 64.36 and 64.43% crude carbohydrate, respectively. The proximate contents of the aronia samples were not significantly different. Among the physicochemical properties, there was also no significant change in total anthocyanin and antioxidant activity. However, Viking had higher total polyphenol contents and ${\alpha}$-glucosidase inhibition activity than Nero. The mineral contents of Al, Fe, Mn, Mg, Ca, Na, K, Cu and Zn in the two aronias were determined. Cu and Zn were not detected in both varieties. With regard to Al, Na and K, Nero was higher than Viking, while Ca was higher in Viking than Nero. From the results, Viking could be suggested to be beneficial for food processing.

Regulation of dendritic arborization by BCR Rac1 GTPase-activating protein, a substrate of PTPRT.

Park, A-Reum,Oh, Daeyoung,Lim, So-Hee,Choi, Jeonghoon,Moon, Jeonghee,Yu, Dae-Yeol,Park, Sung Goo,Heisterkamp, Nora,Kim, Eunjoon,Myung, Pyung-Keun,Lee, Jae-Ran Cambridge University Press 2012 Journal of cell science Vol.125 No.19

<P>Dendritic arborization is important for neuronal development as well as the formation of neural circuits. Rac1 is a member of the Rho GTPase family that serve as regulators of neuronal development. Breakpoint cluster region protein (BCR) is a Rac1 GTPase-activating protein that is abundantly expressed in the central nervous system. Here, we show that BCR plays a key role in neuronal development. Dendritic arborization and actin polymerization were attenuated by overexpression of BCR in hippocampal neurons. Knockdown of BCR using specific shRNAs increased the dendritic arborization as well as actin polymerization. The number of dendrites in null mutant BCR(-/-) mice was considerably increased compared with that in wild-type mice. We found that the function of the BCR GTPase-activating domain could be modulated by protein tyrosine phosphatase receptor T (PTPRT), which is expressed principally in the brain. We demonstrate that tyrosine 177 of BCR was the main target of PTPRT and the BCR mutant mimicking dephosphorylation of tyrosine 177 alleviated the attenuation of dendritic arborization. Additionally the attenuated dendritic arborization found upon BCR overexpression was relieved upon co-expression of PTPRT. When PTPRT was knocked down by a specific shRNA, the dendritic arborization was significantly reduced. The activity of the BCR GTPase-activating domain was modulated by means of conversions between the intra- and inter-molecular interactions, which are finely regulated through the dephosphorylation of a specific tyrosine residue by PTPRT. We thus show conclusively that BCR is a novel substrate of PTPRT and that BCR is involved in the regulation of neuronal development via control of the BCR GTPase-activating domain function by PTPRT.</P>

Si/Co-CoSi₂/reduced graphene oxide ternary nanocomposite anodes for Li-Ion batteries with enhanced capacity and cycling stability

Park, A Reum,Nam, Myeong Gyun,Kim, A-Young,Kim, Kwang Su,Sher Shah, Md. Selim Arif,Lee, Jun Young,Kim, Woo-Jae,Lee, Joong Kee,Yoo, Pil J. Elsevier 2017 Journal of Alloys and Compounds Vol.724 No.-

<P><B>Abstract</B></P> <P>Silicon (Si) is a promising anode material for high-performance Li-ion batteries (LIBs), but it undergoes rapid capacity fading through severe volumetric expansion during Li insertion/extraction. Although alloying Si with various metal sources has been pursued to mitigate the structural deterioration, the resulting materials have shown the intrinsic problem of low electrical conductivity. To address this conflicting issue, here we describe a novel ternary nanocomposite of Si/Co-CoSi<SUB>2</SUB>/reduced graphene oxide (rGO) made using a facile process of mechanical mixing of Si nanoparticles, Co<SUB>3</SUB>O<SUB>4</SUB> microparticles, and rGO nanosheets, followed by carbothermal reduction. Specifically, rGO, which has high electrical conductivity and structural integrity, could work as both a conductive matrix and a reducing agent in forming the Co-CoSi<SUB>2</SUB> phase inside the Si domains during thermal treatment. The proposed ternary nanocomposites exhibited a noteworthy specific capacity of 952 mA h g<SUP>−1</SUP> with 79.3% capacity retention after 80 cycles at a current density of 100 mA g<SUP>−1</SUP>. We attribute the improved electrochemical performance to the increased structural stability offered by the Co-CoSi<SUB>2</SUB> phase and the interconnected conductive framework of the rGO nanosheets. Therefore, we expect our design process for Si/Co-CoSi<SUB>2</SUB>/rGO ternary nanocomposites to be applicable to other materials that can eventually be used as high-performance anodes for the next generation LIBs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new Si/Co-CoSi<SUB>2</SUB>/rGO ternary nanocomposite is suggested as Li-ion battery anodes. </LI> <LI> An electroconductive yet Li-inactive phase of Co-CoSi<SUB>2</SUB> is incorporated into a Si matrix. </LI> <LI> Encompassing rGO nanosheets provide mechanical stability and conductivity during cycles. </LI> <LI> Ternary nanocomposite exhibits excellent electrochemical performance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Si–Mn/Reduced Graphene Oxide Nanocomposite Anodes with Enhanced Capacity and Stability for Lithium-Ion Batteries

Park, A Reum,Kim, Jung Sub,Kim, Kwang Su,Zhang, Kan,Park, Juhyun,Park, Jong Hyeok,Lee, Joong Kee,Yoo, Pil J. American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.3

<P>Although Si is a promising high-capacity anode material for Li-ion batteries (LIB), it suffers from capacity fading due to excessively large volumetric changes upon Li insertion. Nanocarbon materials have been used to enhance the cyclic stability of LIB anodes, but they have an inherently low specific capacity. To address these issues, we present a novel ternary nanocomposite of Si, Mn, and reduced graphene oxide (rGO) for LIB anodes, in which the Si–Mn alloy offers high capacity characteristics and embedded rGO nanosheets confer structural stability. Si–Mn/rGO ternary nanocomposites were synthesized by mechanical complexation and subsequent thermal reduction of mixtures of Si nanoparticles, MnO<SUB>2</SUB> nanorods, and rGO nanosheets. Resulting ternary nanocomposite anodes displayed a specific capacity of 600 mAh/g with ∼90% capacity retention after 50 cycles at a current density of 100 mA/g. The enhanced performance is attributed to facilitated Li-ion reactions with the MnSi alloy phase and the formation of a structurally reinforced electroconductive matrix of rGO nanosheets. The ternary nanocomposite design paradigm presented in this study can be exploited for the development of high-capacity and long-life anode materials for versatile LIB applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-3/am404608d/production/images/medium/am-2013-04608d_0008.gif'></P>

Brief Report : Delayed Allergic Reaction to Secondary Administrated Epidural Hyaluronidase

( A Reum Park ),( Woong Mo Kim ),( Bong Ha Heo ) 대한통증학회 2015 The Korean Journal of Pain Vol.28 No.2

We are reporting a rare case of a delayed hypersensitivity reaction caused by hyaluronidase allergy following a lumbar transforaminal epidural block. Using an intradermal skin test, we have provided evidence that the systemic allergic reaction resulted from hypersensitivity to hyaluronidase. To our knowledge, this is a rare case of a delayed hypersensitivity reaction to epidural hyaluronidase, comprised of an initial exposure to hyaluronidase with no subsequent allergic response in prior block followed by a subsequent delayed reaction to hyaluronidase during a second epidural block. (Korean J Pain 2015; 28: 153-155)