참나무 톱밥을 이용한 표고 지면재배 과정의 주요 미생물 군집 분석

신지혜,윤서연,남지현,구창덕,이동훈,Shin, Ji-Hye,Yun, Seo-Yeon,Nam, Ji-Hyun,Koo, Chang-Duck,Lee, Dong-Hun 한국미생물학회 2015 미생물학회지 Vol.51 No.3

톱밥발효를 이용한 표고버섯의 지면재배방법은 적은 노동력을 소요하고 표고의 생산력을 증대시킬 수 있는 방법이다. 이 방법은 미생물에 의한 참나무 톱밥의 발효과정과 표고균사를 접종하고 생장시키는 과정의 두 단계로 나누어진다. 본 연구에서는 참나무 톱밥을 이용한 표고 지면재배과정의 각 단계에서 우점하는 주요 미생물을 확인하고 효율적인 표고버섯 재배를 위한 정보를 제공하고자 하였다. 발효과정이 진행되며 온도가 상승함에 따라 톱밥의 고온 세균의 비율은 10%에서 80%까지 증가하여 중온성 세균에서 고온성 세균으로 군집의 천이가 확인되었다. 16S rRNA 유전자를 이용한 T-RFLP 방법과 염기서열 분석 방법으로 참나무 톱밥 지면재배과정의 단계별 미생물 군집의 변화를 확인했다. 발효 전 참나무 톱밥시료에서는 중온성 세균인 Enterobacteriaceae 과의 세균이 우점(100%)하였고, 발효가 진행되며 Amycolatopsis (49.0%), Saccharopolyspora (26.5%) 등의 고온성 방선균으로 미생물 군집의 천이가 발생되었다. 특히, Amycolatopsis 속이 최고온도가 유지되는 발효과정 중에도 항상 우점한 결과를 고려하면 발효를 주도하는 미생물이라고 생각된다. 균사생장시기에서는 저온성 세균인 Leuconostoc이 우점(75.0%)하였다. 표고 균사의 활발한 성장을 위해서는 참나무 톱밥의 발효과정이 매우 중요하기 때문에 발효과정에서 우점한 고온성 방선균인 Amycolatopsis 속에 관한 다양한 연구가 필요할 것으로 생각된다. Ground bed cultivation method for shiitake mushroom (Phogo; Lentinula edodes) has been advanced to use the oak-sawdust composted by microorganisms. This method has some advantages in saving the labors and improving productivity. The aim of this study is to analyze microbial community in oak sawdust and to provide the information for efficient cultivation of shiitake mushroom. In process of composting, the proportions of thermophiles increased from 10% to 80%. This results show the microbial community succession from mesophilic bacteria to thermophilic bacteria. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of dominant bacteria coupled with shifts in each stages. The family of Enterobacteriaceae as a mesophilic bacteria were dominant (100%) in oak sawdust sample, but Amycolatopsis (49.0%) and Saccharopolyspora (26.5%) as a thermophilic Actinomycetes were dominant in the last day of composting. In hyphal growth stage, Leuconostoc (psychrophilic bacteria) was dominant (75.0%). The composting process of sawdust is very important for growth of mushroom mycelium. Therefore, there is need for the further study for the Amycolatopsis as thermophilic Actinomycetes.

조선후기 산릉의 여성공간, 나인가가(內人假家)의 변화에 관한 연구

신지혜,Shin, Ji-Hye 한국건축역사학회 2012 건축역사연구 Vol.21 No.5

On behalf of the royal women, SangGung(尙宮:The head of female servants in the palace) and NaIns(內人: Female servants caring for royal families in the palace) were dispatched in order to attend a funeral at the royal tomb. The NaInGaGa(內人假家) is the temporary building for SangGung and NaIns in the royal tomb. It is comprised of lodgings for them and also workrooms and warehouses to prepare ritual offering for the dead King or Queen. In the early Joseon dynasty, the NaInGaGa was utilized until a funeral at the royal tomb. Since 1674, NaInGaGa for the 3 years-period lamentation was started constructing separately. At these processes, the plan and placement of NaInGaGa was changed. This study based on the SanReungDoGam-EuiGwae (山陵都監-儀軌: The report on constructing royal tomb). The SanReungDoGam-EuiGwae written since 1800 have illustration about NaInGaGa. The illustration and explanation about NaInGaGa become a important clue that make suppose detailed space of NaInGaGa.

산양유의 체세포수 등급 설정에 관한 연구

신지혜,정석근,한기성,장애라,채현석,유영모,안종남,우광태,최석호,이완규,함준상,Shin, Ji-Hye,Jeong, Seok-Geun,Han, Gi-Sung,Jang, Ae-Ra,Chae, Hyun-Seok,Yoo, Young-Mo,Ahn, Chong-Nam,Woo, Kwang-Tae,Choi, Seok-Ho,Lee, Wan-Kyu,Ham, Jun-Sang 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.2

The aim of this work was to establish the standard for goat milk somatic cell counts. The data were obtained from MGEN, which were collected from Dec. 2006 to Nov. 2007. A total of three hundred and forty somatic cell counts from 12 goat milk farms were analyzed. A goat milk grading system by somatic cell count is proposed; less than 1,000,000/mL, 1,000,000-1,500,000/mL, 1,500,000-2,000,000/mL, 2,000,000/mL-2,500,000/mL, and over 2,500,000/mL. Under the grading system, the ratio of first grade goat milk would be 26.2%, and that of the fifth grade would be 11.8%. The first grade ratio is low and the fifth grade ratio is high compared to the cow milk grading system. It is expected that somatic cell counts of domestic goat milk will be improved by the proposed grading system.

음식물 쓰레기 퇴비화 과정에 따른 세균군집 구조의 변화

신지혜,이진우,남지현,박세용,이동훈,Shin, Ji-Hye,Lee, Jin-Woo,Nam, Ji-Hyun,Park, Se-Yong,Lee, Dong-Hun 한국미생물학회 2009 미생물학회지 Vol.45 No.2

퇴비화 과정은 유기성 폐기물을 비료와 같은 유용한 자원으로 전환하는 생물학적 과정이다. 본 연구에서는 음식 물 쓰레기를 2달 동안 퇴비화시켜 세균군집의 변화를 조사하였다. 온도의 변화를 기준으로 하여 퇴비화 과정은 1단계($2\sim55^{\circ}C$), 2단계($55\sim97^{\circ}C$), 3단계($50\sim89^{\circ}C$)로 나뉘었다. 각 단계별 총세균수는 1단계 $1.66\times10^{11}$ cell/g, 2단계 $0.29\times10^{11}$ cell/g, 3단계 $0.28\times10^{11}$ cell/g으로 관찰되었다. 또한 총세균수에 대한 고온미생물의 비율은 초기에 33% 였으나 2단계 시료에서 최대비율인 89%로 증가하였다. 16S rRNA 유전자를 대상으로 T-RFLP 방법과 염기서열 분석방법을 이용하여 세균군집의 구조가 퇴비화 과정에 따라 변화됨을 확인할 수 있었다. 초고온인 2단계의 세균군집의 발달은 스타터 접종의 영향을 받았으며, Bacillus 및 Pseudomonas와 유연관계가 가까운 세균군집이 퇴비화 과정을 이끄는 주요 미생물임을 확인하였다. Composting is a biological process converting solid organic waste into valuable materials such as fertilizer. The change of bacterial populations in a composting reactor of food waste was investigated for 2 months. Based on shifts in temperature profile, the composting process could be divided into the first phase ($2^{\circ}C\sim55^{\circ}C$), the second phase ($55^{\circ}C\sim97^{\circ}C$), and the third phase ($50^{\circ}C\sim89^{\circ}C$). The number of total bacteria was $1.66\times10^{11}$ cell/g, $0.29\times10^{11}$ cell/g, and $0.28\times10^{11}$ cell/g in the first, second, and third stages, respectively. The proportions of thermophiles increased from 33% to 89% in the second stage. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of bacterial community structure was coupled with shifts in composting stages. The structure of bacterial community in the ultra-thermophilic second stage reflected that of seeding starter. The major decomposers driving the ultra-thermophilic composting were identified as phylotypes related to Bacillus and Pseudomonas.

감마선 조사에 의한 분유 단백질의 항원성 저감화

신지혜,정석근,한기성,장애라,채현석,유영모,안종남,이주운,조철훈,이완규,함준상,Shin, Ji-Hye,Jeong, Seok-Geun,Han, Gi-Sung,Jang, Ae-Ra,Chae, Hyun-Seok,Yoo, Young-Mo,Ahn, Chong-Nam,Lee, Ju-Woon,Jo, Cheo-Run,Lee, Wan-Kyu,Ham, Jun-Sang 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.3

The aim of this work was to examine the reduction in antigenicity of milk proteins in powdered milk by gamma irradiation which is increasingly used for food safety. Skim milk powder samples were exposed to irradiation doses of 1, 5, and 10 kGy. A greater reduction of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein was found than ${\alpha}_{S0}$-casein and ${\beta}_{A2}$-casein by capillary electrophoresis. Competitive indirect ELISA and passive cutaneous anaphylaxis tests using guinea pigs showed a reduction in antigenicity of powdered milk by 10kGy gamma irradiation. These results indicated that gamma irradiation reduce allergenicity of milk proteins by structural changes of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein, and can be useful for dairy products.

조선후기 영악전(靈幄殿) 기능수용에 따른 정자각 평면변화 고찰

신지혜,Shin, Ji-Hye 한국건축역사학회 2009 건축역사연구 Vol.18 No.4

The "Jeongjagak(丁字閣, T shaped building)" was important building from Goryeo Dynasty to Joseon Dynasty. For long period, the scale and form of the building had been changing bit by bit. The change of building results from the function. As the Jeongjagak accepted the functions of the Yeongakjeon(靈幄殿), there appeared changes of plane. The main function of Yeongakjeon was suppling space for the dead king's coffin. The Yeongakjeon was not built in the first year of King Sukjong(1674). At that time, the Jeongjagak was responsible for the function of the Yeongakjeon as an alternative. Starting from this, the Jeongjagak was used as space for the dead king's coffin. Because the coffin should place on from south to north, it demanded long inner space in south-north direction. Therefore the effort to make enough length in south-north direction was begun from the first year of King Sukjong(1674). In order to solve the problem, the Toigu(退構) was made from the reign of King Sukjong to the 28th year King Youngjo(1852). The Toigu was temporary inner space which was made in one compartment at the Baewichung(拜位廳, the part of the Jeongjagak). But the length of the Jeongjagak's south-north direction was reduced to 61.8m(20尺6寸) at the 33th year of King Youngjo(1857) when the "Gukjosangryebopyon(國朝喪禮補編)" was completed. Also it extended to 84m(28尺) during the reign of King Jeongjo(1774~1800). Following these process of extension and reduction, the length was standardized as 72m(24尺) at the reign of King Sunjo(1800~1834). These facts explains that the main cause of plane change at the Jeongjagak was acceptance of functions that was used as space for the dead king's coffin. Also, the important points of change at the Jeongjagak were the first year of King Sukjong, the 33th year of King Youngjo and the first year of King Sunjo. When it was the first year of King Sukjong and the 33th year of King Youngjo, there were two national funerals. Because of concern about the increasing labor and tax of the nation, the scale of the Jeongjagak was changed to decreasing size. Due to the improvement of drawings and annotation on a Eugwe(儀軌) at the first year of King Sunjo, the size of Jeongjagak was standardized.

뇌교경색 이후 발생한 편마비, 현훈 및 딸꾹질 치험 1례

신지혜,남해인,조윤영,선승호,백태현,김선혁,Shin, Ji-hye,Nam, Hae-in,Cho, Yoon-young,Sun, Seung-ho,Baek, Tae-hyun,Kim, Seon-hyeok 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.2

Objective: : This clinical study reports on the effect of Sunghyangjungki-san-gami and Banhabaeckchulchunma-tang-gami on hemiplegia, dizziness, and hiccupping caused by pontine infarction.Methods: This study was performed on one patient with hemiplegia, dizziness, and hiccupping caused by pontine infarction. The patient was treated by Sunghyangjungki-san-gami and Banhabaeckchulchunma-tang-gami from 6 January to 2 March 2015. The National Institutes of Health stroke scale (NIHSS), motor grade, and numerical rating scale (NRS) were used to assess the effectiveness of this treatment.Results: After treatment, the patient’s discomfort decreased significantly, and NIHSS, motor grade, and NRS scores were all improved.Conclusion: This clinical study suggests that traditional Korean medicine may be effective in treating hemiplegia, dizziness, and hiccupping caused by pontine infarction.

Gigabit Ethernet-PON을 위한 IPACT 매체접근제어 방식의 성능분석

신지혜,이재용,김병철,Shin Ji hye,Lee Jae yong,Kim Byung chul 한국통신학회 2005 韓國通信學會論文誌 Vol.30 No.3B

본 논문에서는 gigabit Ethernet-PON의 상향 트래픽 제어를 위해 제안 된 Interleaved Polling with Adaptive Cycle Time (IPACT) 방식을 살펴보고 IPACT의 gated service와 limited service에 관한 성능을 수학적으로 분석하였다. 성능분석 방법은 IPACT polling system으로 모델링하고, mean value analysis를 사용하였다. Arrival rate λ의 값을 크기에 따라 세 구간으로 나누곤 구간마다의 수학적 분석과정을 제시하였다. 분석결과 gated service와 limited service의 평균 패킷 지연시간, 평균 큐 사이즈, 평균 싸이클 시간 등을 구하고, 수학적 성능분석의 정확성을 검증하기 위해 시뮬레이션을 수행하여 수학적 분석결과와 시뮬레이션 결과가 상당히 일치함을 확인하였다. 기존에 IPACT의 성능평가는 시뮬레이션에만 의존하였는데, 이 방법은 시간과 노력이 많이 필요하였지만, 수학적 성능분석은 성능지표를 단시간 내에 다양하게 구할 수 있어서 시스템 설계에 널리 활용될 수 있을 것이다. In this paper, we examine Interleaved Polling with Adaptive Cycle Time (IPACT) algorithm which was proposed to control upstream traffic for Gigabit Ethernet-PONs, and we analyze the performance of the gated service and the limited service of the IPACT mathematically. For the mathematical performance analysis, we model IPACT algorithm as a polling system and use mean-value analysis. We divide arrival rate λ value into three regions and analyze each region accordingly. We obtain average packet delay, average queue size and average cycle time of both the gated and the limited service. We compare analytical results with simulation to verify the accuracy of the mathematical analysis. Upon now, simulation analysis have been used to evaluate the performance of EPONs, which require much time sud effort. Mathematical analysis can be widely used in the design of EPON systems since system designers can obtain various performance results rapidly.

동해 울릉분지 메탄 하이드레이트 퇴적토의 미생물 군집 특성

신지혜,남지현,이진우,이동훈,Shin, Ji-Hye,Nam, Ji-Hyun,Lee, Jin-Woo,Lee, Dong-Hun 한국미생물학회 2014 미생물학회지 Vol.50 No.3

가스 하이드레이트는 높은 지구 온난화 잠재력을 가지고 있는 메탄가스를 해수 또는 대기 중으로 유입시킬 수 있어 전 지구적 탄소순환과정과 기후 변화에 중요한 역할을 한다. 따라서 해양 또는 대기로 방출되는 메탄의 90% 이상을 미생물 반응을 통해 산화시킬 수 있는 혐기적 메탄산화 과정이 매우 중요하다. 본 연구에서는 동해 울릉분지내 메탄 가스 하이드레이트 퇴적토에 서식하는 미생물 군집의 mcrA 유전자와 16S rRNA 유전자를 분석하였다. 혐기적 메탄산화 고세균(Anaerobic methane oxidizer: ANME) 군집의 수직적 분포를 조사한 결과, 표층과 황산염 메탄전이대(Sulfate methane transition zone: SMTZ)에서는 ANME-1 그룹이, high methane 층에서는 ANME-2c 그룹이 우점하였다. 16S rRNA 유전자를 이용한 고세균의 군집분석 결과, 혐기적 메탄산화가 일어나는 지역에서 주로 발견되는 marine benthic group-B가 50% 이상의 비율로 우점하였다. 세균의 경우 질산염을 환원시킬 수 있는 세균이 SMTZ (Halomonas 속: 56.5%)와 high methane 층(Achromobacter 속: 52.6%)에서 우점하였으며 황산염 환원 세균 군집은 확인되지 않았다. 동해 울릉분지 메탄가스 하이드레이트의 혐기적 메탄산화과정은 일반적으로 해양 퇴적토에서 알려진 혐기적 메탄산화 고세균과 황산염 환원 세균과의 공생에 의한 반응이 아닌 혐기적 메탄산화 고세균과 질산염 환원세균에 의한 반응이 주도할 것이라 생각된다. Gas hydrates play a significant role in the global carbon cycle and climate change because methane, a greenhouse gas, can be released from the dissociation of gas hydrate. Anaerobic oxidation of methane (AOM) is an important process that consumes more than 90% of the methane released into the hydrosphere and atmosphere. In this study, the microbial community associated with the methane gas hydrate sediment in the Ulleung basin, East Sea of Korea (UBGH) was analyzed by phylogenetic analysis of the mcrA and 16S rRNA gene libraries. A vertical stratification of the dominating anaerobic methane oxidizer (ANME)-1 group was observed at the surface and the sulfate methane transition zone (SMTZ). The ANME-2c group was found to be dominant in the high methane layer. The archaea of marine benthic group B, which is commonly observed in the AOM region, accounted for more than 50% of the identifications in all sediments. Nitrate reducing bacteria were predominant at SMTZ (Halomonas: 56.5%) and high methane layer (Achromobacter: 52.6%), while sulfate reducing bacteria were not found in UBGH sediments. These results suggest that the AOM process may be carried out by a syntrophic consortium of ANME and nitrate reducing bacteria in the gas hydrates of the Ulleung Basin of the East Sea.

비염의 침 치료에 대한 최근 임상 연구 동향 - RCT, Review 중심으로 -

신지혜,이장천,김기봉,Shin, Ji-Hye,Lee, Jang-Cheon,Kim, Ki-Bong 대한한방소아과학회 2012 대한한방소아과학회지 Vol.26 No.1

Objectives The purpose of this study is to investigate recent clinical studies on effect of acupuncture for rhinitis in other countries. We have analyzed the studies on effect of acupuncture for rhinitis within randomized controlled trial(RCT), reviewed for 11 years (from 2001 to 2011). Methods The search database includes PubMed. To narrow the search, the following key search terms were used: 'rhinitis, acupuncture'. The search was limited to the publication date from 2001 to 2011. Results Total 20 studies were selected: RCT(13), review(5), case report(1), and cost effectiveness study(1). In RCT, 9 studies used acupuncture only, and 8 studies of them have revealed that acupuncture is effective on rhinitis. In review, 3 studies are systematic review, 2 studies are no systematic review. Among systematic review, 2 studies have revealed partial effectiveness of acupuncture for rhinitis, but they suggested the necessity of further studies. Conclusions Domestic studies on effect of acupuncture for rhinitis are necessary. For more accurate results, controlled, large scale and well designed studies are recommended.