HaCaT 세포에서 Lactobacillus 혼합배양액 추출물이 아토피관련 케모카인 발현에 미치는 효과

홍수정 ( Soo-jeong Hong ),이원재 ( Won-jae Lee ),조을화 ( Eul-hwa Jo ),안성훈 ( Seong-hun Ahn ) 대한경락경혈학회 2017 Korean Journal of Acupuncture Vol.34 No.2

Objectives : Recently the case of lactobacillus mixture culture fluid appliment was reported. In this study, anti-inflamation effects and anti-allergy effects were studied by stimulus of lactobacillus mixture culture fluid extracts in HaCaT cells. Methods : The atopic dermatitis were induced by TNF-α and interferon-γ in HaCaT cells. TARC/CCL17, MDC/CCL22, RANTES/CCL5 and ROS production were investigated to explain anti-inflamation and allergy effects of lactobacillus mixture culture fluid with cell-enzyme-linked Immunosorbent assay in 450 nm, 485 nm, 535 nm with spectro-fluorometer. Results : The extracts of lactobacillus mixture culture fluid were decreased TARC/CCL17, MDC/CCL22, RANTES/CCL5 expressions and ROS production with a concentration dependent manner. Conclusions : The effects mechanism of Lactobacillus mixed culture fluid for atopic dermatitis symptoms were considered to be explain anti-inflamation and allergy effects via control of cytokine, chemokine and ROS production, and the fluid could be applied in skin cells directly. But classified AD symptom degrees reported in clinical case before as Reaction Period, Reduction Period, Effect Period, Reproduction Period and Rebound Period could not be explained. Further study will be expected.

위암환자의 수술 후 합병증 발생에 영향을 미치는 인자

홍수정(Soo-Jung Hong),고정원(Jeong Won Ko),김종흥(Jong Heung Kim) 대한외과학회 2004 Annals of Surgical Treatment and Research(ASRT) Vol.67 No.5

소화성 궤양 천공 환자에서 응급 수술 후 사망률에 영향을 미치는 위험 인자

고정원(Jeong Won Ko),홍수정(Soo-Jung Hong),반주영(Joo Young Ban),김종흥(Jong Heung Kim) 대한외과학회 2004 Annals of Surgical Treatment and Research(ASRT) Vol.67 No.5

보행량에 따른 보행전용존 권장폭 산출식

정우진(Jeong, Woo Jin),홍수정(Hong, Su Jeong),김희수(Hee soo Kim),오흥운(Oh,Heung Un) 대한교통학회 2018 대한교통학회 학술대회지 Vol.78 No.-

본 연구의 목적은 보행만을 위한 공간인 보행전용존의 권장폭 산출식을 제시하여 근린간선지역의 보행랴엥 따른 보행전용존 권장폭을 제시하는 것이다. 국내외 문헌 검토를 통해 최대 보행량, 보행자간 평균간격, 보행자 1인 점유폭, 보행자 평균통행속도를 고려하여 보행전용존 폭 산출식을 도출한다. 도출된 산출식을 통해 근린간선지역 보행전용존의 권장폭을 제시한다. 근린간선지역의 보행전용존 폭은 보행행태가 일반걸음으로 보행량이 24명/분이하일 때 1.4m, 보행량이 24~30명/분일 때 1.7m, 보행량이 30~40명/분일 때 2.3m, 보행량이 40~50명/분일 때 2.8m, 보행량이 50~60명/분일 때 3.4m, 보행량이 60~70명/분일 때 4.0m, 보행량이 70~80명/분일 때 4.5m로 제시한다. 결론적으로 본 연구에서는 보행행태를 반영한 보행밀도를 통해 보행량에 따른 근린지역 보행로의 보행전용존 권장폭을 산출하고 있다.

전방충돌경보(FCW)의 교통안전 증진효과 추정

김형규,이수범,이혜린,홍수정,민혜령,Kim, Hyung-kyu,Lee, Soo-beom,Lee, Hye-rin,Hong, Su-jeong,Min, hye-Ryung 한국ITS학회 2021 한국ITS학회논문지 Vol.20 No.2

자율주행의 핵심기술인 첨단 운전자 지원 시스템(Advanced Driver Assistance Systems) 중 대표기술인 전방충돌경보(Forward Collision Warning)를 대상기술로 선정하여, 주행시뮬레이션 실험 기반의 교통사고 예방효과를 추정하였다. 효과척도로 ①인지반응시간(s) ②감속도(m/s2) ③충돌여부(회)로 선정하여, 전방충돌경보 미설치시와 설치시의 변화량 측정하였다. 실험 시나리오는 운전자 전방의 차량의 급정거하는 시나리오(1)과 옆차로에서 차량이 끼어드는 시나리오(2)를 진행하였으며, 주간/야간으로 구분하였다. 분석결과, 전방충돌경보장치를 설치하였을 경우, 인지반응시간(s)이 감소하였으며, 감속도(m/s2)는 감소하였다. 운전자의 위험상황을 빠르게 감지하여 여유로운 감속을 할 수 있게 되었으며, 그에 따른 전방충돌횟수도 감소한 것으로 분석되었다. 향후 운전자의 운전성향을 반영하고 실험 시나리오를 다양화하면, ADAS의 설치효과를 증대시키고 다른 기술의 효과추정에도 활용될 수 있을 것이다. The Forward Collision Warning, a representative technology of the Advanced Driver Assistance Systems, was selected as the target technology. The cognitive response time, deceleration, and impact were selected as the measures of effectiveness. And the amount of change with and without the Forward Collision Warning was measured. The experimental scenarios included a sudden stop event (1) of the vehicle in front of the driver and an event (2) in which the vehicle intervened in the next lane. All experiments were divided into day and night. As a result of the analysis, response time and the deceleration rate decreased when the forward collision warning system was installed. It was analyzed that the driver's risk situation could be detected quickly and the number of front-end collisions could be reduced as a result. Reflecting the driver's operating habits and diversifying the experimental scenarios will increase the installation effectiveness of ADAS and be used to estimate the effectiveness of other technologies.

인체의 난관수종액이 생쥐의 배아발달에 미치는 영향: II. 포배기내의 세포 수에 미치는 영향

궁미경,전진현,송상진,송지홍,홍수정,유근재,손일표,김정욱,강인수,Koong, Mi-Kyoung,Jun, Jin-Hyun,Song, Sang-Jin,Song, Ji-Hong,Hong, Soo-Jeong,Yoon, Keun-Jae,Song, Il-Pyo,Kim, Jeong-Wook,Kang, Inn-Soo 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

In our previous study, we observed that hydrosalpingeal fluid (HSF) adversely effect mouswe embryo development and hatching. The aim of this study was to evaluate the effect of HSF as assessed by the blastocyst development rate (BDR) and by cell counting in vitro. HSF was collected from ninie patients undergoing salpingoneostomy to correct hydrosalpinx. Two-cell embryos were obtained from superovulated ICR mice. T6 medium and $T6{\pm}0.4%$ bovine serum albumin were used as control media. T6 medium containing 10% or 50% HSF and 100% HSF from each patient were used as test media. Nine to 15 embryos were cultured in micro drops prepared from each of these media. To assess the total cell number within each blastocyst, the blastocysts were fixed and stained with Hoechst 33342 to facilitate cell counting. The mean BDR in two control media were 88.89% and 85.40%. The mean BDR in media containing 10%, 50%, 100% HSF were 85.87%, 89.58% and $75.57%^*$, respectively ($^*$: p<0.05). The overall mean cell count $({\pm}SEM)$ in control media were $87.6{\pm}9.65\;and\;90.12{\pm}11.38$. The BDR was affected adversely only by 100% HSF and not in media containing 10% or 50% HSF. Mean cell counts were decreased significantly only in blastocysts cultured 100% HSF ($63.8{\pm}13.66$; p<0.01) but not in blastocysts cultured in 10% or 50% HSF ($91.3{\pm}12.44\;and\;82.9{\pm}18.27$, respectively). Thus, it is concluded that HSF has no embyotoxic effect but has a mildly negatively effect on embryonic growth and development.

고식적 체외수정시술과 난자 세포질내 정자주입술에 의해 태어난 아이의 주산기 결과 및 선천성 기형 발생빈도의 비교 연구

임정은,유근재,이종표,이문섭,현우영,전진현,홍수정,송지홍,송인옥,백은찬,최범채,손일표,궁미경,강인수,전종영,박인서,Lim, Jeong-Eun,Yoo, Keun-Jai,Lee, Jong-Pyo,Lee, Moon-Seob,Hyun, Woo-Young,Jun, Jin-Hyun,Hong, Soo-Jeong,Song, Ji-Hong,Song, In-Ok,Paik, 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

The safety of ICSI as a novel procedure of assisted fertilization may be assessed by the health of the baby born. In order to evaluate the safety of ICSI, perinatal outcome and congenital anomaly of the babies born after ICSI were compared with those of babies born after IVF (control group). We analysed the clinical data from the obstetric and pediatric records, including the information obtained through telephone. The results are as follows; Mean gestational age $({\pm}SEM)$ and birth weight in singleton pregnancy were $38.8{\pm}1.9$ weeks and $3209.7{\pm}501.9gm$ in IVF group, $39.0{\pm}2.2$ weeks and $3289.9{\pm}479.5gm$ in ICSI group, respectively. Mean gestational age and birth weight in twins were $36.8{\pm}2.1$ weeks and $2512.8{\pm}468.0gm$ in IVF group, $36.5{\pm}2.8$ weeks and $2492.7{\pm}537.1gm$ in ICSI group. In IVF group, perinatal mortality rates were 8.5 in singletons and 56.6 in twins; for the ICSI singletons and ICSI twins, the perinatal mortality rates were 11.6 and 49.0, respectively. The incidence of congenital malformations was 3.6% (8/224) in IVF group and 2.1% (4/188) in ICSI group, there was no statistical difference (p>0.05, Fisher's exact test). The incidence of major congenital anomalies was 0.9% (2/224; pulmonary artery hypoplasia, renal cystic dysplasia) in IVF group and 1.1% (2/188; holoprosencephaly, Cri du chat syndrome) in ICSI groups (p>0.05, Fisher's exact test). Similarly, there was no significant difference in incidence of minor congenital anormalies 2.7% (6/224) in IVF group and 1.1% (2/188) in ICSI group respectively (p>0.05, Fisher's exact test). In conclusion, there was no difference in the perinatal outcome and the incidence of congenital anomalies between the babies born after ICSI and those after conventional IVF.

거대 태반혈관종의 임상적 의의

이환견 ( Hwan Kyoun Lee ),강창성 ( Chang Seong Kang ),홍수정 ( Soo Jeong Hong ),박선희 ( Sun Hee Park ),김은성 ( Eun Sung Kim ),한호원 ( Ho Won Han ),홍성란 ( Sung Ran Hong ) 대한주산의학회 1997 Perinatology Vol.8 No.2

생리혈에 존재하는 탈락된 자궁내막조직의 체외배양 시 나타나는 변화와 특성에 관한 연구

전진현(Jin Hyun Jun),궁미경(Mi Kyoung Koong),강인수(Inn Soo Kang),양광문(Kwang Moon Yang),홍수정(Soo Jeong Hong),김문규(Moon Kyoo Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.1

목적: 자궁내막증의 체외 모형에 적합한 생리혈 내의 탈락된 자궁내막조직을 체외배양하면서 나타나는 변화와 특성에 관하여 살펴보고자 하였다. 연구 방법: 정상적인 생리주기를 갖는 10명의 여성으로부터 Wallace catheter를 이용하여 생리혈을 채취하였다. 원심분리 방법으로 생리혈을 세척한 후, 수획한 탈락된 자궁내막조직은 10% fetal bovine serum이 첨가된 Ham's F-10배양액에서 7일 동안 배양하였다. 배양한 자궁내막조직 세포의 특성을 확인하기 위해 cytokeratin과 vimentin에 대한 항체를 이용하여 면역조직학적 염색을 실시하였다. 결과: 생리혈의 평균 양은 0.7ml이였으며, 세척 후 혈액세포와 탈락된 자궁내막조직이 포함된 침전부위의 평균 양은 0.3ml 이였다. 체외배양 한 자궁내막조직의 15% 정도가 배양접시 표면에 부착된 후 증식하였다. 일차적으로 상피세포와 섬유아세포가 배양접시의 표면에 부착하여 증식하였으며, 기질세포들은 상피세포 위에서 증식하였다. 면역조직학적 염색에서 상피세포, 기질세포, 섬유아세포 등이 확인되었으며, 공촛점주사 현미경을 이용한 체외배양된 탈락된 자궁내막조직의 3차원적인 구조 관찰에서 외부에는 상피세포가, 내부에는 기질세포가 존재함을 확인하였다. 결론: 생리혈 내의 탈락된 자궁내막조직의 체외배양 시 상피세포와 기질세포가 밀접한 연관성을 유지하면서 증식하는 것을 관찰하였으며, 이러한 체외배양 체계는 자궁내막증의 병인에 대한 연구에 유용하게 적용될 수 있는 체외 모형으로 사료된다. Objective: To evaluate the viability and the characteristics of shed endometrial tissues obtained from menstrual fluid during in-vitro culture. Methods: The menstrual fluids were collected using Wallace catheter from uterine cavity in 10 women with regular menstruation. The menstrual fluids were washed twice, and the pellets, containing blood cells and shed endometrium, were collected and diluted fivefold with Ham's F-10 medium containing 10% fetal bovine serum. The cell suspension was placed on culture dishes, and cultured for 7 days in an incubator. To evaluate the characteristics of the cultured endometrial cells, immunohistochemical (IHC) staining was performed using anti-cytokeratin and anti-vimentin antibody. Results: The mean volume of menstrual fluids and pellets were 0.7ml and 0.3ml, respectively. Only 15% of the shed endometrial tissues were attached and proliferated in culture dishes, which was considered to have viability. Initially, endometrial epithelial cells and fibroblasts were attached and proliferated, and the area of these cells was increased according to prolong the culture time. Stromal cell colonys were located and proliferated on the epithelial cells. IHC staining showed strongly positive for cytokeratin in epithelial cells and for vimentin in stromal cells. In the confocal microscopic observation of 3-dimensional structure of cultured endometrium, cytokeratin-positive cells (epithelial cells) were located in the pheriphery and cytokeratin-negative cells (stromal cells) inside of the structure. Conclusion: From our study, shed endometrial tissues in menstrual fluid showed meaningful viability and closed relationship between epithelial cells and stromal cells during in-vitro culture. Thus, we suggest that the in-vitro culture system of shed endometrium is a suitable model for researches of endometriosis.

생리혈내 탈락된 자궁내막세포의 유착력에 관한 연구: 자궁내막증의 병인 연구를 위한 실험 모델

궁미경(Mi Kyoung Koong),전진현(Jin Hyun Jun),김은수(Eun Soo Kim),고경남(Kyoung Nam Ko),홍수정(Soo Jeong Hong),송지홍(Ji Hong Song),유근재(Keun Jai Yoo),강인수(Inn Soo Kang) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.12

목적: 자궁내막증의 원인은 아직도 잘 밝혀지지 않았으나, 역류된 자궁내막세포 및 조직이 복강 내로 전이, 착상되어 형성된다는 가설이 지배적이다. 그러나, 실제적으로 복강 내에서 어떤 과정에 의해 자궁내막세포 및 조직이 복막에 유착되고 침윤되어 병변이 발생되는 가에 관하여서는 알려지지 않았다. 따라서 본 연구에서는 양막을 이용하여 생리혈에 존재하는 탈락된 자궁내막조직이 상피층에 유착될 수 있는 가를 알아보고자 하였다. 연구방법: 정상적인 생리주기를 갖는 20명의 여성으로부터 생리주기 제2일이나 제3일에 자궁으로부터 Wallace catheter를 통해 흘러나오는 생리혈을 채취하였다. 양막은 합병증이 없는 만삭분만시의 태반으로부터 채취, 분리하여 사용하였다. 채취된 생리혈은 Ham's F-10 배양액으로 2회 세척한 후 준비된 양막조각 위에서 배양액과 함께 5일간 배양하였다. 그 후 양막에 자궁내막조직이 유착된 부위를 해부현미경 하에서 관찰하였고, 유착부위가 관찰되면 이를 고정하여 조직절편으로 만들어 염색, 관찰하였다. 결과: 생리혈에 존재하는 자궁내막조직의 유착부위는 양막의 세포외 기질층 뿐 아니라 상피층 모두에서 관찰되었다. 유착부위의 크기는 매우 다양하여 현미경적 크기부터 육안 확인 가능한 크기까지 관찰되었다. 결론: 생리혈에 존재하는 자궁내막조직은 이미 탈락되었음에도 불구하고 유착력을 가지고 있으며, 이러한 유착력은 자궁내막증을 일으키는 데 관여할 것으로 사료된다. Objectives: The purpose of this study was to determine whether or not the shed endometrial tissues in menstrual fluid (MF) have adhesive potentials, using human amniotic membrane (AM). Methods: The MF from 20 patients with regular menstruation was collected with Wallace catheter by aspiration from the uterine cavity on the second or third day of the menstrual period. The AM was obtained from the placenta of term delivery without any complication. The MF was washed and diluted fivefold with Hams F-10 medium supplemented with 10% fetal bovine serum. The cell suspension was placed on either epithelial layer (EP) or extracellular matrix layer (ECM) of the AM. After 5 days of culture, the adhesion sites were observed under a stereomicroscope. For histological observation, each cultured AM was prepared for the serial paraffin section. Results: The adhesion sites of endometrial tissues in MF were found both ECM (20/20) and EP (11/20) of the AM. The size of adhesion sites in each AM were highly variable from microscopic to macroscopic size. Conclusion: We found that the shed endometrial tissues in MF have adhesive potential to epithelial layer in addition to extracellular matrix layer of amniotic membrane. This adhesive potential may be related to pathogenesis of endometriosis. We suggest that this culture system can be useful as an in-vitro model for endometriosis.