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        Rapid real-time detection method of ACLSV and ASSVd for apple quarantine field

        허성,정용석 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.2

        Apples are produced using a high-density plantation system using M.9 rootstock in Korea. However, the virus infection rate of apples tends to be high because the rootstocks produced from virus-infected mother stocks were widely used and vegeta�tive propagation based on the grafting is common with scion cultivars. The most efective way to control plant virus and viroid depends on conducting a massively rapid and precise diagnostic inspection. Therefore, this study aims to develop a real-time PCR assay that can be precisely and inexpensively tested for ACLSV and ASSVd. To expand the detection range, we developed a singleplex real-time PCR method targeting the conserved region of ACLSV and ASSVd, respectively. The detection methods using two kinds of fuorescent chemical dye, SYBR Green I and TaqMan probe, have been developed in this study. Comparing these methods developed with two dyes, the assay using SYBR Green I was efcient for detection as much as the method using TaqMan probe and faster. The feld test demonstrated real-time detecting methods developed using SYBR Green I dye were applicable to reliable diagnosis and quantifcation for virus and viroid in quarantine felds.

      • KCI등재

        從業員 스톡옵션 報償의 決定要因에 관한 연구-코스닥 벤처기업을 중심으로-

        허성,지성권 한국회계정보학회 2007 회계정보연구 Vol.25 No.1

        The purpose of this study is to investigate what are the economic and non-economic determinants of stock option compensation granted to employees(non-executive employees). The stock option compensation granted to the employees is an increasing function of the complexity of organization structure and the degree of decentralization of decision making(1). The stock option compensation granted to the employees increases as the investment opportunity set gets larger(2), effective tax rate gets higher(3), borrowing cost of capital increases(4), cash flow shortage gets serious(5), stock return of the firm increases(6). As the variance of stock return increases, the stock option compensation granted to the employees decreases(7).The first and second hypotheses are to test the relationship between employee stock option grants and the measurement difficulty of effort exerted by employees. The third, fourth and fifth hypotheses are to investigate what are the relationship between employee stock option grants and the resource constraints of the firms. The sixth hypothesis is to test whether or not the relative costs of stock option compensation as compared to cash compensation determines the grant of employee stock option. The seventh hypothesis is to test whether or not the relative contribution of employees to the firm value as compared to managers determines the grant of employee stock option. We used four different measures for the dependent variable. The results support second, fourth, fifth, sixth, and seventh hypothesis. 우리나라에 스톡옵션 제도가 도입된 후, 경영자를 대상으로 한 스톡옵션 보상에 대하여는 연구가 있었으나, 종업원을 대상으로 한 연구는 전무한 실정이다. 이것은 스톡옵션을 종업원 보상으로 사용하는 기업이 많지 않기 때문이기도 하며, 주식관련 보상이라는 특성상 종업원 보다는 경영자 보상으로서의 성격이 강하기 때문이기도 하다. 그러나 벤처기업에서는 스톡옵션을 종업원 보상의 한 방법으로 많이 사용하며, 많은 벤처기업이 스톡옵션 보상을 사용하는 추세이다. 본 논문은 코스닥에 상장되어 있는 벤처기업을 대상으로 종업원 스톡옵션 보상을 결정하는 경제적, 비경제적 결정요인이 무엇인지를 규명했다. 독립변수로는 투입노력측정의 난이도, 유동성제약을 해결하기 위한 방법, 보상의 상대적 비용, 그리고 종업원 투입 노력이 미래 기업가치 증대에 공헌하는 상대적 공헌도와 관련된 요인들이 종업원 스톡옵션 보상에 어떠한 영향을 미치는 지를 분석하였다. 종속변수로는 총발행주식수 대비 스톡옵션수의 비율, 종업원 1인당 스톡옵션주식 수, 전체 스톡옵션 대비 종업원 스톡옵션 비율 그리고 블랙-숄즈 가치이다.연구 결과에 따르면 분권화가 잘 되어 있으면 전체 스톡옵션 중 종업원 스톡옵션의 비중이 커진다. 성장성이 높으면 종업원 1인당 스톡옵션의 수가 많아진다. 외부의 자금 조달 비용이 높아지거나 유동성에 제약이 있으면 모든 종속변수에서 유의적인 관련성을 보였다. 주식수익률이 높으면 종업원 1인당 스톡옵션의 수도 많아지며, 전체 스톡옵션 중 종업원 스톡옵션의 비중도 커진다. 주식가경의 변동성이 높아지면 종업원 1인당 스톡옵션의 수가 증가한다. 이상의 연구결과에서 보면 종업원을 위한 스톡옵션 보상은 기업이 처한 유동성제약을 해결하고, 상대적으로 적은 비용으로 큰 보상을 하기 위한 방법으로 사용되었음을 알 수 있다.

      • KCI등재

        High‑resolution melting analysis for identification of apple cultivars using simple sequence repeat markers

        허성,김창수,정용석 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.4

        Apple trees are perennial plant and have a characteristic of requiring long period from seed germination to flowering and fruiting. Most Korean apple cultivars are derived from closely related varieties genetically; consequently, they are distributed into grafted seedlings at juvenile stage. Therefore, it is difficult for them to distinguish by morphological traits due to the high similarities without fruit characteristics. We have integrated the high-resolution melting (HRM) analysis with simple sequence repeat (SSR) marker genotyping to identify thirteen apple cultivars originated from South Korea. The patterns of temperature-shifted melting curves were investigated from HRM analysis. The SSR loci used in this study generated a unique melting curve profile for each cultivar allowing their comparison and classification. The combination of the allelic patterns obtained with the just three SSR markers was able to identify thirteen apple cultivars. These results suggest that SSR markers used in combination with HRM analysis can be a fast and accurate method for high-throughput cultivar identification and biodiversity assessment.

      • KCI등재

        The effect of gamma-irradiation on the changes of photosynthetic efficiency in Kanpei (Citrus unshiu × C. sinensis)

        허성,최정민,Le Anh Tuan,은창호,김인중,정용석 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.2

        Fruit trees are perennial plant to have a characteristic requiring a long juvenile period until fruit set in general. Developing to release a new fruit cultivar is a laborious and cost- and time-consuming work. Especially, citrus breeding is not always feasible due to its cross-incompatible, sterile, and polyembryonic characteristics. Therefore, mutagenesis has been widely applied to citrus breeding to increase mutation frequency and genetic variability enabling development of new genotypes as an alternative method. Recently, various tangor (Citrus unshiu× C. sinensis) cultivars were released through interspecific hybridization. Many attempts have been made to induce mutations in developing tangor cultivars, while their physiological effects have not been verified yet. To investigate the effect of gamma-irradiation exerted on tangor cultivar ‘Kanpei’, its scions were irradiated with 20, 40, 60, 80, and 100 Gy of gamma-rays. Then, we examined the SPAD values between the gamma- irradiated M0 plants comparing with control ‘Kanpei’ (0 Gy). Interestingly, we found that there are variations in SPAD values among different treatments. This implies that mutation breeding should consider different factors other than the target traits.

      • KCI등재

        사과 단과지성 중간모본 "원교 가-단과지 1호"

        허성,황정환,신일섭,신용억,김기홍 한국육종학회 2009 한국육종학회지 Vol.41 No.4

        "원교 가-단과지 1호"는 농촌진흥청 국립원예특작과학원에서 고품질 중생종 "Starkrimson"에 무측지성 "McIntosh Wijick"을 1992년에 교배하여, 2000년부터 2008년까지 그 특성을 조사한 결과, 과실 특성이 뛰어난 계통을 중간모본으로 최종 선발 하였다. 만개기는 "홍로"와 비슷하거나 2일 정도 느리고, 숙기는 "홍로"보다 일주일 정도 느리다. 수세는 중, 수자는 반개 장형이고, 단과지 형성 및 착과량은 "홍로"와 같이 많은 편이 Intermediate parent "Wonkyo Ga-Dangwagi 1" (Malus domestica Borkh.) was released from Fruit Research Division, National Institute of Horticultural & Herbal Science, Korea in 2008. It was derived from the cross between "Starkrimson" and columnar type "McIntosh Wijcik" in 1992 after selection tests from 2000 to 2008. Trees are moderately vigorous and have spreading branching habit. It bears abundant flower buds on one-year-old branches and fruits mainly on spurs or short branches. Harvesting time of this variety is late September, 7 days later than that of "Hongro" in Suwon. The fruit is conical to narrow conical in shape and bright red in skin color. Fruit size is small with weight of 200~240g on an average and fruits have 14ºBx soluble solid and medium acidity. It is moderately susceptible to alternaria leaf spot (Alternaria alternata). "Wonkyo Ga-Dangwagi 1" is a promising apple cultivar and will appeal to young people with good sugar-acid balance.

      • KCI등재

        Validation of MADS‑box genes from apple fruit pedicels during early fruit abscission by transcriptome analysis and real‑time PCR

        허성,정용석 한국유전학회 2019 Genes & Genomics Vol.41 No.11

        Background Fruit abscission in an isolated region called abscission zone (AZ) is regulated by several genes including JOINTLESS, MACROCALYX and SEPALLATA , MADS-box genes, in tomato. Objective The surviving central pedicels and the abscised lateral pedicels were examined in fruit clusters in order to investigate apple MADS-box genes from fruit pedicels of self-abscising apple ‘Saika’ during early fruit abscission. Methods After performing RNA-Seq, transcription profiling was conducted on the MADS-box genes from apple central and lateral pedicels. The JOINTLESS homolog of apple (MdJOINTLESS) was amplified using degenerate primers annealing to a highly conserved domain based on the orthologous genes of various crops, including JOINTLESS gene of tomato. The expression pattern of MdJOINTLESS was investigated in central and lateral pedicles by real-time PCR. Results Some homologs were found which similar to JOINTLESS, MACROCALYX and SEPALLATA of tomato MADS-box genes from transcriptome analysis and RACE. Using phylogenetic analyses with the MADS-box gene family, MdJOINTLESS was classified into the SHORT VEGETATIVE PHASE (SVP) clade that included Arabidopsis and other crops. The expression level of MdJOINTLESS in central pedicel was more than twice as high as that of lateral pedicel. Conclusion In the current study, we could find apple homologs of JOINTLESS, MACROCALYX, SEPALLATA , which were known to regulate pedicel AZ development in tomato. Furthermore, MdJOINTLESS might contribute to auxin gradation, influencing hierarchical ranking of auxin transport between fruit pedicels of self-abscising apple.

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