Ultrasound-Triggered Drug Release of Hydroxyapatite Coated Liposomes

조성근,위태인,하정,조선행,한건,한희동,신병철,Cho, Sung Keun,Wee, Tae In,Ha, Jeung,Cho, Sun Hang,Han, Kun,Han, Hee Dong,Shin, Byung Cheol Korean Chemical Society 2013 대한화학회지 Vol.57 No.4

리포솜은 표적 약물을 봉입하여 병소에 안전하게 전달할 수 있는 약물전달체로서 연구되고 있다. 그러나 일반적인 리포솜은 표적부위에서 약물방출이 제한적인 문제점이 있다. 따라서 본 연구에서는 리포솜의 안정성을 향상시키고 표적부위에서 외부 초음파로부터 약물의 방출을 극대화시키기 위하여 하이드록시아파타이트(hydroxyapatite, HA)가 코팅된 리포솜을 개발하였다. 대조군 리포솜은 인지질과 콜레스테롤을 이용하여 제조하였고, 대조군 리포솜의 표면에 칼슘 아세테이트, 포스포릭에시드, 그리고 25% 암모니아용액을 이용하여 HA를 코팅하였다. 모델 약물로는 독소루비신을 사용하였다. HA코팅 리포솜의 크기는 120 nm 이었고, 약물봉입효율은 95% 이상이었다. 30% 혈장용액 내에서 HA코팅 리포솜의 입자크기는 일정한 상태를 유지하였으며, 대조군 리포솜은 크기가 1.4배 증가하였다. 외부 초음파 자극에 의한 리포솜으로부터 약물 방출을 유도한 후, 방출된 약물의 세포 이입율은 HA 코팅된 리포솜이 3배 이상 대조군 리포솜에 비하여 증가하였다. 본 연구에서는 외부 초음파 자극에 의하여 리포솜으로부터 약물의 방출을 극대화시키기 위한 초음파 민감형 리포솜을 개발하였고, 본 제형은 표적부위에서 약물의 방출을 효과적으로 제어하기 위한 분야에 활용이 가능할 것이다. Liposomes, which can deliver payload at target site, have been studied as drug carrier. However, conventional liposomes have limitation for drug release at target site. Therefore, we developed hydroxyapatite (HA) coated ultrasound sensitive liposomes to increase drug release at target site and to enhance stability in blood stream. Control liposome was prepared using hydrogenated soy phosphatidylcholine (HSPC) and cholesterol, and then we assessed HA coating on the surface of control liposomes using calcium acetate, phosphoric acid, and 25% ammonium solution. Doxorubicin was used as a model drug. Size of HA coated liposomes was 120 nm and encapsulation efficiency of doxorubicin in liposomes was up to 95%. Size of HA coated liposomes are not changed in 30% serum solution, however, the control liposomes was 1.4 fold increased. After ultrasound triggered drug release from liposomes, intracellular efficiency of drug released from HA coated liposomes was 3 fold increased compared to control liposomes. In this study, we developed ultrasound sensitive liposomes to enhance drug release, which will be applied in controlled drug release at disease site.

임부의 치주염이 조산 및 저체중아 출산에 미치는 영향

하정은 ( Jung Eun Ha ) 한국치위생학회(구 한국치위생교육학회) 2016 한국치위생학회지 Vol.16 No.2

The purpose of the review article is to investigate the influence of periodontal diseases on preterm birth(PTB) and low birth weight(LBW). PTB and LBW are the main risk factors of infant mortality and a major public health problem. PTB is defined as delivery at less than 37 weeks and LBW is less than 2,500 grams. Over Approximately 60 percent of perinatal mortality results from PTB or LBW. Although the causes of PTB and LBW are not fully understood, infection is the leading cause of PTB and LBW. Periodontal diseases are serious disease burdens because they are caused by bacterial endotoxin, inflammatory reaction, and cytokine. The periodontal diseases are the predisposing factors of cerebrovascular and cardiovascular diseases including atherosclerosis. Over the past 15 years, previous studies revealed that periodontitis had adverse outcomes including PTB and LBW in pregnancy.

『남훈태평가(南薰太平歌)』 시조에 나타난 근대성 연구

하정의 ( Jeong Eui Ha ) 문창어문학회 2010 문창어문논집 Vol.47 No.-

This study has the purpose of looking into the aspects of modernity shown in the sijo 『Namhwoon teapyeong songs』, which was compiled aiming at commercial potential and popularity in the 19th-century social change imminent to the modern age. The 19th century was the period for wrapping up the middle age and setting the scene for the modern period with too complex aspects to illuminate socially and culturally from a single angle of view. That is, it was the period of going through epochal changes from middle into modern times, such as developing commerce and industry, forming classes of new merchants and rich farmers, shaking status system, etc. 『Namhwoon teapyeong songs』is a commercial block book compiled in such socio-cultural environment. Investigating the modern nature of this collection of songs will be significant in approaching complicated and multifacetedhistory of sijo literature in the 19th century, together with the future course of sijo chang (traditional narrative songs) contrasted with melody chang, exclusive belongings to upper-class culture. The result of this study can be summarized as the following: First, Chapter II dealt with the social and cultural backgrounds that brought the 『Namhwoon teapyeong songs』into being. First, through socio-cultural changes in the latter 19th century, the motive to form the 『Namhwoon teapyeong songs』was diagnosed in detail. Then, culture of publishing commercial block book was discussed in the historical context of literature, illuminating the modern meaning of literary commercialization. Second, in Chapter III, as a prerequisite for grasping the modern aspects shown in the sijo 『Namhwoon teapyeong songs』, the term of``modern age/modernity``was defined, establishing the preconditions for detailed analysis of the work (subjectivity, actuality, rationality, popularity and traits of prose). In full-dress discussion, based on the preconditions presented earlier, composition of 『Namhwoon teapyeong songs』was divided into works of accommodation, modification and first productto consider the modern aspects. Unlike other collections of songs, 『Namhwoon teapyeong songs』was the one reflecting the active attitude of stressing the populace`s autonomous will of practice and individual awareness in adaptation and creation. It was also seeking for freedom in expressing personal emotions and desires with concrete perception of reality. Besides, publishing a block book based on commercial potential and popularity, use of conversational style exposing the atmosphere of the scene as it was, and open attitude of acceptance oriented toward traits of prose are interpreted as a head start for aiming at modern times shedding the middle age. Seen from above, 『Namhwoon teapyeong songs』is considered in an important position to reveal the modern meaning of 19-century sijo literature in the sense that it aims at the new, generally taking off the earlier formality of sijo literature and reflecting open thinking.

복수 (複數) 의 근대와 민족문학

하정일(Jeong Il Ha) 민족문학사학회·민족문학사연구소 2000 민족문학사연구 Vol.17 No.-

N/A

일제말기 임화의 문학비평과 이중과제론

하정일(Ha Jeong-il) 한국근대문학회 2009 한국근대문학연구 Vol.10 No.2

임신중 체중증가 및 임신전 체질량지수와 주산기 예후의 연관성

하정이 ( Jeong Yi Ha ),김효진 ( Hyo Jin Kim ),강창성 ( Chang Seong Kang ),박성철 ( Sung Chul Park ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.10

목적 임신중 체중증가 및 임신전 체질량지수와 주산기 예후의 연관성을 확인하고자 한다. 연구방법 2001년부터 2009년까지 비합병 만삭분만한 3,428예의 한국인 산모를 대상으로 하였다. 임신전 체질량지수를 기준으로 저체중(< 18.5 kg/m2), 정상체중(18.5-22.9 kg/m2), 과체중(23.0-24.9 kg/m2), 비만군(≥ 25.0 kg/m2) 산모군 등 4개의 군으로 나누었고, 임신중 체중증가의 제1, 제2, 제3 사분위 수에 따라 4개의 군으로 분류하였다. 임신중 체중증가 및 임신전 체질량지수와 특정 주산기 예후의 독립적 연관성은 로지스틱 회귀분석을 사용하였다. 결과 임신중 체중이 많이 증가할수록, 임신전 체질량지수가 클수록 부당경량아의 위험도는 낮았고, 미산모 제왕절개수술 및 부당과량아의 위험도는 높았다. 결론 임신중 체중증가 및 임신전 체질량지수와 주산기 예후의 연관성이 있었다. 과다하거나 과소한 임신중 체중증가와 연관된 산모나 신생아 합병증을 예방하기 위해서 한국인 산모를 위한 임신중 체중증가 권장치를 설정하기 위해 다기관 공동연구가 필요할 것으로 생각된다. Objective To investigate an association of gestational weight gain (GWG) and prepregnancy body mass index (BMI) with perinatal outcomes. Methods The sample included 3,428 Korean patients who had uncomplicated singleton term pregnancy from 2001 to 2009. The patients were categorized into four groups according to prepregnancy BMI; underweight (< 18.5 kg/m2), normal weight (18.5-22.9 kg/m2), overweight (23.0-24.9 kg/m2) and obese (≥ 25.0 kg/m2) and were also categorized into four groups according to the 25th, 50th, 75th percentile of GWG. Adjusted associations of GWG and prepregnancy BMI with perinatal outcomes were estimated by logistic regression analyses. Results The greater GWG and the heavier prepregnancy BMI decreased the risk of small for gestational age and increased the risk of cesarean section for nulliparas and large for gestational age. Conclusion There was an association of GWG and prepregnancy BMI with perinatal outcomes. To prevent maternal or neonatal complications associated with excessive or inadequate GWG, a multicenter study is needed to establish a Korean standard value for the recommended range of GWG.

일부 시판세치제의 비교치아마모도에 관한 연구

하정은 ( Jung Eun Ha ),강연주 ( Yeon Joo Kang ),진보형 ( Bo Hyoung Jin ),백대일 ( Dai Il Paik ),배광학 ( Kwang Hak Bae ) 대한예방치과·구강보건학회 2011 大韓口腔保健學會誌 Vol.35 No.1

Objectives. The purpose of this study was to measure the relative tooth abrasivity of the domestic dentifrices marketed in Korea. Methods. The authors measured the relative tooth abrasivity of thirty-two types of dentifrices. Assessment of tooth dentin abrasivity produced by different dentifrices was carried out on extracted bovine teeth in the laboratory and was tested by the surface profile measurement method. Calcium pyrophosphate was used as a reference abrasive and was assigned a score of 1.000 for the profilometry method. Results. The degree of the relative tooth abrasivity of the dentifrices varied from 0.048 to 1.095. The dentifrices were divided into 4 groups according to the Euclidean distance data mining. Conclusions. The abrasivity of all the tested dentifrices was at an acceptable level for consumers.

평면 소자형 락테이트 바이오센서

하정한(Jeonghan Ha),허황(Hwang Huh),강태영(Tae Young Kang),이용석(Yong Seok Lee),윤순호(Soon Ho Yoon),신정원(Jungwon Shin),남학현(Hakhyun Nam),차근식(Geun Sig Cha) 한국분석과학회 2006 분석과학 Vol.19 No.6

일제 말기 임화의 생산문학론과 근대극복론

하정일 ( Jeong Il Ha ) 민족문학사학회·민족문학사연구소 2006 민족문학사연구 Vol.31 No.-

현대일본어에 있어서 실례와 무례의 의미확장 -개념적 중심성과 기능적 중심성-

하정일 ( Jeong Il Ha ) 한국일어일문학회 2015 日語日文學硏究 Vol.93 No.1

본 논문은 실례와 무례의 개념적 중심성 및 기능적 중심성의 의미확장을 분 석하고 두 가지 용법의 의미확장이 상이점을 유발하는 원인에 대해서 고찰하였 다. 실례와 무례의 기본적인 의미는 예의를 벗어나는 것, 이른바 비례 또는 결례 라는 개념적 중심성을 지닌다. 따라서 두 가지의 용법은 공통으로 비례 또는 결례라는 개념적 중심성으로부터 “사회적 규범에 관한 부정적 평가”, “체면침해 행위에 관한 부정적 평가” 라는 “부정적 평가” 용법의 기능적 중심성의 의미확 장이 나타난다. 그러나 실례 용법은 “인사”, “주의환기”, “말걸기”, “서론제기” 라는 기능적 중심성의 “의례” 용법의 의미확장이 나타나지만, 무례 용법에서는 이러한 용법이 나타나지 않는다. 또한, 실례 용법은 사과의 대상에 따라서, 화자 의 청자에 대한 “예의에 관한 사과”, “과실에 관한 사과”, “공손전략에 관한 사 과” 라는 기능적 중심성의 “사과” 용법의 의미확장이 나타난다. 이른바 비례 또는 결례에 어긋나지 않는 “의례” 용법의 의미확장이 예의를 벗어나는 행동이 아닌 단순한 “과실에 관한 사과”, “공손전략에 관한 사과” 의 의미확장을 가능하 게 한다. 한편, 무례는 “의례” 용법의 의미확장을 지니지 않으므로 “과실에 관한 사과”, “공손전략에 관한 사과” 의 의미확장이 나타나지 않고, 단지 “예의에 관 한 사과” 만의 의미확장이 나타난다. 이러한 기능적 중심성의 의미확장에서의 상이점은, 두 가지 용법에서의 시간적인 축의 인식에 기인한다. This paper analyzes the extension of the meanings of shitsurei and burei from conceptual centrality to functional centrality, thereby inquiring into the cause of the difference in expansion between their respective meanings. The two words shitsurei and burei basically mean to lack good manners, thus possessing the conceptual centrality of bad or improper manners. The usage of the two words then expanded from the conceptual centrality of bad or improper manners to the functional centrality of “negative evaluation,” as in “negative evaluation in terms of social norms” or “negative evaluation due to face threatening acts.” However, the meaning of shitsureiexpanded in the direction of the usage of “rituals” with the functional centrality of “farewells,” “attracting attention,” “calling,” and “exordium,” while on the other hand, this did not occur with the usage of burei. Further, the usage of shitsurei is, depending on the subject of apology, expanded to that of “apology” with the functional centrality of “apology in rituals,” “apology for mistakes,” and “apology for volition” extended by the speaker to the listener. That is to say, the expansion of the meaning of shitsurei to the usage of “rituals,” rather than focusing on making bad or improper manners, allows for the expansion to merely an “apology for mistakes,” not for behaviors lacking good manners. On the other hand, since the meaning of burei did not expand to the usage of “rituals,” it did not expand to “apology for mistakes” or “apology for volition,” but only to the usage of “apology in rituals.” Thus, the difference in the expansion of meaning to functional centrality between the two usages is caused by their perceptions of the temporal axis.