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경구투여한 V. vulnificus 백신의 면역원성 및 감염방어효능
이나경(Na Gyong Lee),정상보(Sang Bo Jung),안보영(Bo Young Ahn),김영지(Young Gi Kim),이윤하(Youn Ha Lee),전영중(Young Joong Jeon),박완제(Wan Je Park) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.2
Vibrion vulnificus is an estuarine gram0negative human pathogen that affects people with chronic hepatitis, alcoholic cirrhosis, diabetes mellitus or other underlying diseases, V. vulnificus infection is mediated primarily by consumption of raw fish or by exposure of pre-existing wounds to seawater, causing permanent tissue damages or fatal septic shock. We have been developing a vaccine against V. vulnificus composed of whole cell lysate of a V. vulnificus O-antigen serotype 4 strain. Oral administration of the V. vulnificus vaccine elicited a high serum antibody response in rabbits. The induced antibodies were reactive not only to the homologous strain but also to heterologous O-antigen serotype strains, indicating cross-reactivities among serotypes. Western blot analysis revealed that the antibodies are mainly specific for outer membrane proteins (OMPs) and reacted equally well with OPMs purified from 9 O-antigen serotypes The rabbit antisera showed opsonophagocytic killing activity against heterologous strains as well as the homologous strain. Passively transferred rabbit antisera into mice were protective against a lethal V. vulnificus infection. These data demonstrate that oral administration of the V. vulnificus infections, suggesting that this vaccine preparation could be used to develop an oral vaccine against V. vulnificus. Keywords □ V. vulnificus. oral vaccine, immunogenicity, protective efficacy
D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구
곽미선,이승구,정상철,서승현,이재흥,전영중,김영호,성문희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-
To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two therrnophiles, Bacillus sp. LK-I and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-0l, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.
염료의 색도 제거능력이 우수한 백색부후균 분리 및 특성 연구
오광근,김현수,조무환,채영규,전영중 한국산업미생물학회 1999 한국미생물·생명공학회지 Vol.27 No.6
한국에서 자생하는 버섯으로부터 염료의 색도 제거능력이 우수한 균주를 분리하기 위하여 각종 염료를 함유한 평판배지 상에서 성장성과 색도 변화를 관찰한 결과 우수한 균체 성장과 색도 제거능력을 지닌 3가지 균주를 최종 선별하였으며, 선별된 균주의 특성을 조사하여 Trametes sp. CJ-105, Pleurotus sp. CJ-212, Fomes sp. CJ-315로 동정하였다. 이 균주들은 각각 remazol brilliant blue R 및 Acid red 264를 함유한 액체배지에서 배양한 결과, 배양 2∼8일째에 거의 색도가 제거되었고 리그닌 분해효소인 laccase의 활성이 다량 존재하였다. 선별된 균주의 색도 제거능력과 laccase, LiP, MnP의 활성은 상관관계가 있는 것으로 나타났으며, 염료의 색도 제거는 리그닌 PAH 및 PCP의 분해에도 좋은 활성을 가지며 난분해 물질의 분해에도 리그닌 분해효소의 활성이 상관관계가 있음을 확인하였다. 따라서, 선별된 균주가 향후 염색폐수의 색도 제거, 난분해 물질의 분해 또는 매립지 침출수의 처리등에 이용될 수 있을 것으로 기대된다. Several white-rot fungi collected from the mountains of Korea were evaluated for their ability to decolorize azo, polymeric, and reactive dyes. Strains CJ-105, CJ-212 and CJ-315, identified as Trametes sp., Pleurotus sp. and Formes sp., respectively, showed higher potential for decolorization of those dyes in either solid or liquid media. For Trametes sp. CJ-105, 100 ppm of Remazol Brilliant Blue R and 500 ppm of Acid Red 264 were completely decolorized after 2 days under liquid culture. The dominating ligninolytic enzyme existing in the culture broth was laccase (E.C. 1.10.3.2). Also, Pleurotus sp. CJ-212 and Fomes sp. CJ-315 showed similar patterns in decolorization of Remazol Brilliant Blue R and Acid Red 264. The extent of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic enzymes produced by each strain. In addition to that Trametes sp. CJ-105 was highly effective in degradation of polycyclic aromatic hydrocarbons and pentachlorophenol by the activity of the ligninolytic enzymes produced. In this study, we found that white-rot fungi, Trametes sp. CJ-105(KFCC 10941), Pleurotus sp. CJ-212(KFCC 10943) and Fomes sp. CJ-315(KFCC 10942), were effective in decolorizing a wide range of structurally different synthetic dyes, as well as some chemical compounds which are known to be hardly degradable.
신현철,하구선,김성준,조영제,전영중,이재흥 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.6
Corynebacterium glutamicum CH 1516(L-leucine 생산주)를 사용하여 7ℓ 발효조에서 배양온도 및 pH, 산소전달속도 등을 최적화한 결과 각각 30℃ , 7.0, 0.21 kmole O_2/㎥·hr이었다. 산소전달속도가 0.19 kmole O_2/㎥·hr보다 낮은 조건에서는 상당량의 lactic acid가 축적되었고 0.23 kmole O_2/㎥·hr보다 높은조건에서는 glutamic acid가 생성되고 PCV가 크게 증가하였다. 한편 1200ℓ 실험공장에의 L-leucine 생산을 위해 7ℓ 발효조에서 최적화된 배양조건을 적용하여 산소전달속도를 지표로 공정확대를 실시한 결과 7ℓ 발효조와 거의 대등한 결과를 얻을 수 있었으며 산화환원전위 -150∼-170mV에서 L-leucine이 왕성하게 생산되었다. The effects of pH, temperature and oxygen transfer rate(OTR) on L-leucine fermentation were investigated employing Corynebacterium glutamicum CH 1516 in 7ℓ fermentor. The optimum pH, temperature and OTR were determined to 7.0, 30℃ and 0.21 kmole O_2/㎥·hr, respectively. For the values of OTR lower than 0.19 kmole O_2/㎥·hr a significant amount of lactic acid was accumulated, while the packed cell volume(PCV) was rapidly increased at higher OTR values above 0.23 kmole O_2/㎥·hr and glutamic acid was produced to some extent. Scale-up studies for L-leucine fermentation which was carried out in 1200ℓ pilot scale fermentor reaffirmed the results of 7ℓ fermentation. The optimum redox potential value for L-leucine production was found to be -150 to -170 mV.