인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

봉약침 자극이 사상체질별 건강인의 심박변이도, 맥파, 뇌혈류에 미치는 영향

이상민,김구,오승윤,권영미,주종천,Lee, Sang-Min,Kim, Koo,Oh, Seung-Yun,Kwon, Young-Mi,Joo, Jong-Cheon 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.1

1. Objectives To evaluate effects of bee venom acupuncture on cardiovascular system and differences according to each constitution. 2. Methods Heart rate variability, pulse wave and the velocity of cerebral blood flow were measured before bee venom acupuncture(BVA), right after and after 30 minuets, had been applied to 20 subjects. 3. Results 1. BVA did not have effects on measurement variables of heart rate variability. 2. BVA had effects on pulse wave, showing total time, radial augmentation index up and height of percussion wave, time to percussion wave, sum of pulse pressure down. 3. BVA did not have effects on the cerebral blood flow velocity when considering not Sasang Constitution. 4. Considering Sasang Constitution, BVA demonstrates different responses in time to preincisura wave, mean blood flow velocity, peak systolic velocity and end diastolic velocity. 4.Conclusion From those results, the following conclusions are obtained. Cause BVA alters pulse wave and makes differences in the cerebral blood flow velocity according to Sasang Constitution. Various methods of BVA treatment are needed considering Sasang Constitution.

기존학교 시설개선사업의 통합적 추진을 통한 효율화 방안 -K-TEGS 평가기법을 통한-

이상민,김성중,맹준호,Lee, Sang-Min,Kim, Seong-Joong,Meang, Joon-Ho 한국교육녹색환경연구원 2011 교육·녹색환경연구 Vol.10 No.1

The importance of education and training facilities and education facilities on the relationship between effective as demonstrated in many studies can be very big. In this study, the status of existing school facilities improvement project is done individually, based on the systematic integration of the improvement project was to evaluate the feasibility. Furthermore, it was based on looking for ways to effectively pursue. Training School Facilities Improvement Program to be competitive, the country continued to be pursued systematically and very large scale as a whole must be a great national project. So much to promote efficient business environment, quality of education for children with detractors of the national budget to ensure efficient and most importantly in terms of economic investment that can be. In this study, the proposed K-TEGS existing school facilities, such as the development of techniques for the assessment based on this effort to promote an efficient business should be done on a continuous basis.

소화기질환(消化器疾患)에 사용(使用)된 약주요법(藥酒療法)에 대한 문헌적(文獻的) 고찰(考察)

이상민,강재춘,Lee Sang-Min,Kang Jae-Chun 대한예방한의학회 1999 대한예방한의학회지 Vol.3 No.1

To investigate the documental records of the medi-alcohol theraphy on the Gastrointestinal disease, the 33 kinds of medical books editioned from the old to the present were used. the results were followed Many herbs used on the Gastrointestinal disease were sequently Atractylodis Macrocephalae Rhizoma, Zingiberis Rhizoma, Ginseng Radix. The herbs of invigorating and warming body, regulating the flow of qi etc. were many occupied(used) in treating the Gastrointestinal disease. In analyzing treatment symptom as medi-alcohol theraphy were sequently many used abdominal pain, inappetence, gastrointestinal cancer, indigestion, fatigue, vomiting, diarrhea, abdominal dropsy. The kinds of using medi-alcohol on the Gastrointestinal disease were totally 120 ones.

사료의 $\alpha-cellulose$ 함량이 조피볼락 Sebastes schlegeli의 성장, 사료 효율 및 체성분에 미치는 영향

이상민,이종윤,LEE Sang-Min,LEE Jong Yun 한국양식학회 1994 韓國養殖學會誌 Vol.7 No.2

사료의 $\alpha-cellulose$ 함량이 조피볼락의 성장, 사료 효율, 영양소 이용 효율 및 체조성에 미치는 영향을 조사하기 위해 209 전후의 조피볼락 치어를 대상으로 사료의 $\alpha-cellulose$ 함량이 $0\~20\%$가 되도록 총 5종의 실험 사료를 제조하여 10주간 사육 실험한 결과는 다음과 같다. $\alpha-cellulose$ 함량 $10\%$ 첨가구까지는 대조구와 유의차없이 성장 및 사료 효율이 양호하였고, $\alpha-cellulose$ $15\%$이상 첨가 실험구들은 유의하게 낮은 값을 보였다(P<0.05). 일간 먹이 섭취량은 $\alpha-cellulose$ $15\%$ 이상 첨가구에서 유의하게 높은 결과를 보였다(P<0.05). 영양소 축적효율 중 단백질 및 에너지 축적 효율은 $\alpha-cellulose$ $10\%$ 첨가구까지 대조구와 유의차 없이 좋은 결과를 보인 반면, 지질 축적 효율은 a-cellulose 함량이 증가함에 따라 계속 감소하였다 (P< 0.05). 전어체의 단백질 함량은 실험 구간별로 차이가 없었지만, 지질 함량은$\alpha-cellulose$ $10\%$ 첨가구에서 유의하게 높은 값을 보였다(P<0.05). Experimental diets containing 5 different levels of a-cellulose $(0\%,\;5\%,\;10\%,\;15\%\;or\;20\%)$ were fed to juvenile Korean rockfish (20.5g of initial mean weight) for 10 weeks to study their ability to utilize dietary $\alpha-cellulose$. Weight gain, feed efficiency, and protein and energy retention efficiency in the fish fed diets containing $0\%,\;5\%,\;or\;10\%$ of $\alpha-cellulose$ were significantly higher than those in the fish fed diets containing $15\%\;or\;20\%$ of $\alpha-cellulose$ (P<0.05). Whole body lipid content of the fish fed $10\%$ $\alpha-cellulose$ diet were significantly higher than that of fish fed other diets (P<0.05). The results suggest that the optimum dietary fiber levels for Korean rockfish would be less than $10\%$.

소양인(少陽人) 임신오조(姙娠惡阻) 환자를 형방사백산(荊防瀉白散)으로 치료한 치험례

이상민,배영춘,유관석,주종천,서은희,Lee, Sang-Min,Bae, Young-Chun,Yoo, Gwan-Seok,Joo, Jong-Cheon,Seo, Eun-Hee 사상체질의학회 2004 사상체질의학회지 Vol.16 No.2

1. Objectives Hyperemesis Gravidarum is one of common symptoms clinically and caused by pregnancy. This is defined as vomiting sufficiently pernicious to produce weight loss, dehydration, acid-base imbalance. In severe case, it also leads to neurologic abnormality including confusion, gate disturbance, nystagmus. We have tried researching effect of herbal medicines, Soyangin Hyungbangsabaek-san. 2. Methods To heal the Hyperemesis Gravidarum, we used Soyangin Hyungbangsabaek-san. 3. Results Hyperemesis Gravidarum of a Soyangin patient was treated with a herb-medicine, and change of her subjective symptom & general condition was managed. 4. Conclusions The constitutional treatment with herb-medicine (Soyangin Hyungbangsabaek-san) was efficacious against Hyperemesis Gravidarum. Further study on management of this disease is needed.

고온관 누설에 의한 가압열충격 사고시 원자로 용기의 건전성 평가를 위한 결정론적 파괴역학 해석

이상민,최재붕,김영진,박윤원,정명조,Lee, Sang-Min,Choi, Jae-Boong,Kim, Young-Jin,Park, Youn-Won,Jhung, Myung-Jo 대한기계학회 2002 大韓機械學會論文集A Vol.26 No.11

In a nuclear power plant, reactor pressure vessel (RPV) is the primary pressure boundary component that must be protected against failure. The neutron irradiation on RPV in the beltline region, however, tends to cause localized damage accumulation, leading to crack initiation and propagation which raises RPV integrity issues. The objective of this paper is to estimate the integrity of RPV under hot leg leaking accident by applying the finite element analysis. In this paper, a parametric study was performed for various crack configurations based on 3-dimensional finite element models. The crack configuration, the crack orientation, the crack aspect ratio and the clad thickness were considered in the parametric study. The effect of these parameters on the maximum allowable nil-ductility transition reference temperature ($(RT_{NDT})$) was investigated on the basis of finite element analyses.

파워 분배망을 고려한 디지털 회로 시스템의 설계와 분석

이상민,문규,위재경,Lee, Sang-Min,Moon, Gyu,Wee, Jae-Kyung 대한전자공학회 2004 電子工學會論文誌-SD (Semiconductor and devices) Vol.41 No.4

This paper presents the channel analysis considering power distribution network(PDN) system of PCB. For achieve the target PDN system we proposed the useful design approach for acquiring the characteristic target of power distribution network in overall frequency ranges. The proposed method is based on the hierarchical approach related to frequency ranges and the path-based equivalent circuit model to consider the interference of the current paths between the decoupling capacitors and the board through it is a lumped model for fast and easy calculation, experimental results show that the proposed model is almost as precise as the numerical analysis. The analysis of PDN system shows that although the effective inductance of package dominatly affects the power noise and the signal transfer through data channel, the board PDNs also can not be neglected for achieving the accurate channel signaling. Therefore, we must design concurrently the chip, package, and board from the initial spec design of high speed digital system. 이 논문은 PCB의 PDN(Power Distribution Network) 시스템을 고려한 채널 분석을 나타내었다. 설계자가 원하는 PDN 시스템을 설계하기 위하여, 전체 주파수 범위의 PDN이 요구하는 임피던스를 얻는 유용한 설계방법을 제안하였다. 제안된 방법은 주파수 영역과 관계된 계층적 배치 접관방식과 보트와 decoupling 커패시터 사이의 current 흐름의 간섭을 고려한 path-based equivalent 회로를 기본으로 하였다. 비록 빠르고 쉬운 계산을 위한 lumped model일지라도, 실험 결과는 제안된 모델이 numerical 분석처럼 거의 정확함을 보였다. PDN 시스텐의 분석은 패키지 인덕턴스가 파워 노이즈, 데이터 채널을 통한 신호 이동에 영향을 받는다는 것을 보여주고 있으나, 보드 PDN 또한 정확한 채널 신호를 위해 무시할 수 없다는 것을 보여준다. 따라서 설계자는 반드시 초고속 디지털 시스템의 첫 스팩 설계로부터 보드, 패키지, 칩 등을 동시에 디자인을 해야 한다.

학교시설 수선주기 설정에 관한 연구

이상민,Lee, Sang Min 한국교육녹색환경연구원 2018 교육·녹색환경연구 Vol.17 No.2

본 연구는 안전성, 노후도, 경제성 등 시설 여건을 종합적으로 고려하여 개선이 필요한 중장기적인 사업물량 및 사업예산 규모를 추정할 수 있도록 하기 위해 국가수준의 시설개선 기준(수선주기)을 설정하여 체계적인 시설개선계획 수립을 통한 적정수준의 교육환경 확보에 기여할 수 있도록 하는 것을 주목적으로 한다. 적정 수선주기는 기본적으로 최근 2년간 교육청에서 실제 집행한 교육환경개선사업의 실적자료를 분석하여 직전 보수년도와 사업시행연도를 근거로 수선주기 기초자료를 산출하였다. 이를 근거로 17개 시도교육청 실무자를 대상으로 델파이 조사를 실시하여 시도교육청의 의견을 수렴하였으며 최종 적정 수선주기는 관련규정과 실적자료, 델파이조사 결과를 종합적으로 검토하여 설정하였다. In Korea, it is difficult to secure student health and safe educational environment due to the rapid deterioration of many school facilities built in the 70s and 80s in the past. As a result, through the improvement of school facilities, social needs for safe schools from various disasters, accidents, Is increasing. The purpose of this study was to establish a standard for the establishment of the national and school facilities for the mid - to long - term plan for the improvement of the old school facilities, and to propose a framework for mid - to long - term and rational planning that can be used by the national and local government offices. The purpose of this study is to establish a mid - and long - term plan for the improvement of old school facilities. The appropriate repair cycle for the improvement of the school facilities set up in this study can be utilized as a standard for the mid - and long - term planning for improvement of the school facilities in the Ministry of Education and the School of Education. To this end, a standard model, It is possible to roughly estimate the overall improvement in the mid- to long-term facilities at the national and regional levels.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.