Root-associated bacteria influencing mycelial growth of Tricholoma matsutake (pine mushroom)

오승윤,임영운 한국미생물학회 2018 The journal of microbiology Vol.56 No.6

Tricholoma matsutake is an ectomycorrhizal fungus usually associated with Pinus densiflora in South Korea. Fruiting bodies (mushrooms) of T. matsutake are economically important due to their attractive aroma; yet, T. matsutake is uncultivatable and its habitat is rapidly being eradicated due to global climate change. Root-associated bacteria can influence the growth of ectomycorrhizal fungi that co-exist in the host rhizosphere and distinctive bacterial communities are associated with T. matsutake. In this study, we investigated how these bacterial communities affect T. matsutake growth by isolating bacteria from the roots of P. densiflora colonized by ectomycorrhizae of T. matsutake and co-culturing rootassociated bacteria with T. matsutake isolates. Thirteen species of bacteria (27 isolates) were found in pine roots, all belonging to the orders Bacillales or Burkholderiales. Two species in the genus Paenibacillus promoted the growth of T. matsutake in glucose poor conditions, likely using soluble metabolites. In contrast, other bacteria suppressed the growth of T. matsutake using both soluble and volatile metabolites. Antifungal activity was more frequent in glucose poor conditions. In general, pine rhizospheres harbored many bacteria that had a negative impact on T. matsutake growth and the few Paenibacillus species that promoted T. matsutake growth. Paenibacillus species, therefore, may represent a promising resource toward successful cultivation of T. matsutake.

버섯 종 다양성 연구의 최근 방향

오승윤 한국버섯학회 2018 버섯 Vol.22 No.1

사상체질별 관격(關格)치료 약물인 파두(巴豆), 감수(甘遂), 과신(瓜莘)의 문헌 고찰

오승윤,이시우,주종천,Oh, Seung-Yun,Lee, Si-Woo,Joo, Jong-Cheon 사상체질의학회 2008 사상체질의학회지 Vol.20 No.2

1. Objectives I investigated the effects and mechanisms of Semen Tiglii(ST, 巴豆), Radix Euphorbiae Kansui(REK, 甘遂) and Pedicelus Melo(PM, 瓜莘) in the view of Sasang Constitutional Medicine. 2. Methods I analyzed the literature about oriental medicine and Sasang Constitutional medicine related to herbal Medicine. The contents analyzed were efficacious disease, dosage, usage, side effect, toxicity. 3. Results The first, similarities of ST, REK and PM are improving Gwangyuk, the Chest Discomfort with Constipation, Dysuria and Nausea, toxic, used in urgency, asministered by itself and used shaped powder. The second, ST and REK used the diseases related water and food metabolism in Soumin and Soyangin. PM used the diseases related Qi and fluid metabolism in Taeumin. The third, diarrhea caused by ST and REK is symptom to retrieve the diseases related water and food metabolism. The vomiting caused by PM is symptom to retrieve the diseases related Qi and fluid metabolism. 4. Conclusions The ST, REK and PM is good herbal medicine (() retrieve the urgency symptom caused by the body unbalance in Sasang Constitutional Medicine. The proper dosage is essential because of toxicity of drugs.

P2 버섯 종 다양성 연구의 최근 방향

오승윤 한국버섯학회 2018 버섯 Vol.22 No.2

동의수세보원(東醫壽世保元) 신정(新定) 처방(處方) 약재(藥材)의 약침 안전성 문헌 조사

오승윤,김구,권영미,이시우,장은수,주종천,Oh, Seung-Yun,Kim, Koo,Kwon, Young-Mi,Lee, Si-Woo,Jang, Eun-Su,Joo, Jong-Cheon 사상체질의학회 2011 사상체질의학회지 Vol.23 No.1

1. Objectives: Pharmacopuncture according to Sasang Constitutional Medicine is new field of constitutional medicine. We investigated safety of pharmacopunctures to develop this treatment. 2. Methods: We searched for papers in fifteen Korean oriental medical journals and four online searching portal articles. Hepatotoxicity, nephrotoxicity and general toxicity tests were investigated about one hundred thirty kinds of pharmacopunctures. 3. Results: Toxicity test about 36 kinds of 130 pharmacopunctures were investigated. 1) Hepatotoxicity and nephrotoxicity test of pharmacopunctures were searched in 11 of 38 Soeumin herbs, 8 of 36 Soyangin herbs, 5 of 44 Taeeumin herbs and 1 of 12 Taeyangin herbs. From among these herbs, toxicity was detected in Radix aconiti lateralis preparata(附子), Fructus alpiniae oxyphyllae(益智仁), Semen crotonis(巴豆), Radix bupleuri(柴胡) and Semen armeniacae amarum(杏仁) pharmacopunctures. 2) General toxicity test of pharmacopunctures were searched in 8 of 38 Soeumin herbs, 9 of 36 Soyangin herbs, 6 of 44 Taeeumin herbs and 2 of 12 Taeyangin herbs. from among thes herbs, toxicity was detected in Radix aconiti lateralis preparata(附子), Radix ginseng(人蔘), Semen crotonis(巴豆), Rhizoma seu radix notopterygii(羌活), Radix aralia cordatae(獨活), Semen armeniacae amarum(杏仁) and Radix scutellariae(黃芩) and Cortex acanthopanacis(五加皮) pharmacopunctures. 4. Conclusions: From these results, we must decide extraction method, concentration and dose carefully to use the toxic pharmacopuncture. More toxicity test and further study is needed about pharmacopuncture treatment in Sasang Constitutional Medicine.

야생버섯에서 분리한 미기록종 버섯기생균 Sepedonium laevigatum 보고

오승윤 한국버섯학회 2022 한국버섯학회지 Vol.20 No.4

Fungi that infect mushrooms and cause diseases are called mycoparasitic fungi. Understanding the diversity of mycoparasitic fungi in wild mushrooms is important for the mushroom industry, as they can parasitize cultivated mushrooms. However, few studies have been conducted on the diversity of mycoparasitic fungi in wild mushrooms in South Korea. Upon surveying mushroom flora, we found a mycoparasitic fungus and analyzed its strain using molecular phylogeny and morphological examination. The strain was identified as Sepedonium laevigatum, which is an unrecorded species in South Korea. In this study, we described the cultural and microscopic characteristics of this strain of fungus.

국내 데이터베이스 검색을 통한 암성통증의 약침치료 임상연구 동향

오승윤,신뢰,주종천,박수정,Oh, Seung-Yun,Shen, Lei,Joo, Jong-Cheon,Park, Soo-Jung 대한암한의학회 2020 大韓癌韓醫學會誌 Vol.25 No.2

Objectives: The effectiveness of acupuncture on cancer pain is relatively well established, while there have been few clinical studies on pharmacopuncture on that. This research was aimed to investigate a trend of pharmacopuncture treatment for cancer pain and to propose information for further research. Methods: Clinical studies on pharmacopuncture for cancer pain were collected from 5 Korean medical databases. And the studies were analyzed by classifications of cancer, chief complaints, interventions, treating skills, additional treatments, treatment session, outcome measures, results and adverse effects. Results: The literatures were 8 case reports and 1 randomized controlled trial. Interventions were pharmacopunctures of soyeom, mountain ginseng, Harpagophyti Radix, snake venom, Trionycis Carapax, bee venom, Aconitum Ciliare Decaisne. Treatment skills, procedures, and indications of each pharmacopuncture were different. An adverse effects of bruising, bleeding, mild chilling, sore throat and pain were mentioned on snake venom and bee venom treatment. Conclusions: An appropriate interventions and treatment skills should be selected according to the type of cancer pain. And treatment procedures to ensure safety must be developed.

흡입화상으로 유발된 만성 기관지염의 태음인 마황정천탕(麻黃定喘湯) 치험 1례

오승윤,곽시라,Oh, Seung-Yun,Gwak, Si-Ra 사상체질의학회 2020 사상체질의학회지 Vol.32 No.3

Objectives To report an improved case with Taeeumin Mahwangjeongcheon-tang on the patient with chronic bronchitis caused by an inhalation injury. Methods The patient had been suffered from cough, sputum, dyspnea, and chest discomfort for 15 years. He was diagnosed as Taeeumin Esophageal Cold Pattern and was given Mahwangjeongcheon-tang according to Sasang constitutional medical theory. The clinical asthma measurement scale in oriental medicine(CAMSOM-V), quality of life questionnaire for adult Korean asthmatics(QLQAKA), and Spirometry were performed to evaluate the improvement of his symptoms. Results The clinical asthma measurement scale in oriental medicine(CAMSOM-V) improved from 16 to 14 points and the quality of life questionnaire for adult Korean asthmatics(QLQAKA) improved from 55 to 67 points. In spirometry, forced vital capacity improved from 3.07 to 3.59, and forced expiratory volume in 1sec improved from 2.59 to 2.84 after 4 weeks. Conclusion This report suggests that Mahwangjeongcheon-tang is effective for chronic bronchitis.

조위승청탕(調胃升淸湯)이 심박수 미세 변화에 미치는 영향

오승윤,이시우,길은영,주종천,Oh, Seung-Yun,Lee, Si-Woo,Kil, Eun-Young,Joo, Jong-Cheon 사상체질의학회 2008 사상체질의학회지 Vol.20 No.2

1. Objectives When we give Jowiseungcheong-tang to Taeumin patients, analyzing a change of their autonomic nervous system. It is measured by the heart rate variability(HRV) analyzer. 2. Methods We give Jowiseungcheong-tang to ten Taeumin patients for three days and examine the HRV at first time and three days later. 3. Results and Conclusions We compared two HRV results. The value of RRNN(average of normal RR intervals), SDNN(the standard deviation of all normal RR intervals), RMSSD(the root mean square of successive differences between the normal heart beats) have a downward tendency. Norm HF(normalized high frequency power) have a upward tendency. norm LF (normalized low frequency power) and LHR(LF/HF ratio) have a downward tendency. It means hypofunction of autonomic nervous system. But all results are not significant. We think that restudy is needed with more patients and longer periods afterward.

간전이 바터팽대부암의 항암화학요법에 병용한 한의치료 1례

오승윤 대한암한의학회 2023 大韓癌韓醫學會誌 Vol.28 No.1

Objectives: To report the reduction of adverse effects of chemotherapy and improvement in the quality of life in recurrent liver metastasis by Korean medicine treatments. Methods: In 2018, a 75-year-old male patient underwent surgery for primary cancer in the ampulla of Vater. In 2021, he was diagnosed with recurrent liver metastasis. Following the failure of tumor treatment with gemcitabine/cisplatin and the development of severe side effects, he decided to discontinue chemotherapy. Subsequently, with a significantly enlarged liver tumor, he resumed capecitabine/oxaliplatin treatment, alongside moxibustion, acupuncture, and herbal prescriptions from August 2021 to August 2023. The changes of chief complaints, abdominal CT, and laboratory findings were investigated. Results: After combined treatment of Korean traditional medicine and chemotherapy, the mass was decreased. Laboratory findings, and chief complaints of hand-foot syndrome, fatigue, abdominal distension, nausea and anorexia were improved. Conclustions: This case study suggests that Korean traditional medicine is effective in enhancing anticancer effects, suppressing the side effects of chemotherapy, and improving general conditions.