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자궁경부에서 인유두종 바이러스 검출을 위한 다중 중합효소 연쇄반응법 (Multiplex PCR)과 Hybrid Capture법의 비교 분석
한세준 ( Sei Jun Han ),김산 ( San Kim ),강민창 ( Min Chang Kang ),임주성 ( Ju Seong Lim ),안태규 ( Tae Gyu Ahn ),이병래 ( Byung Rai Lee ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.1
목적 : 인유두종바이러스감염의 검출법으로 알려져 있는 HPV hybrid capture systerm과 multiplex-PCR법을 자궁경부 시료에서 HPV 감염의 검출법으로 비교하였다. 연구 방법 : 91명의 한국여성중에서 자궁경부 시료를 채취하여 HPV hybrid capture Ⅰ법과 multiplex-PCR 방법에 의하여 HPV를 검출하였다. 결과 : Multiplex-PCR에 의한 유병률은 hybrid capture Ⅰ 방법에 의한 것보다 더 Objective : To compare the multiplex-PCR and hybrid capture methods for detection of Human papillomavirus (HPV) in uterine cervical swab samples. Methods : This study determined the HPV infection and its risk group (subtype) in uterine cervical swab sampl
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사람唾液 Superoxide Dismutase의 精製 및 性狀
이봉재,이병래,전현우,양종대,이근배 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1
The purification and some properties of superoxide dismutase(O_(2-)-O_(2-) oxidoreductase, ECI. 15.1.1) of human saliva have been studied. Human saliva was collected from medical students, male and female together with smoking male students. The activity of superoxide dismutase was measured by the method of McCord and Fridovich. The superoxide dismutase was purified by ammonium sulfate precifitation, DEAE-cellulose and sephadex C-100 column chromatography. The results are summerized as follows : 1. Human saliva contain measurable amounts of superoxide dismutase. 2. The activities of superoxide dismutase. were 0.72U/㎖ in male and 0.73U/㎖ in female saliva. The activity of this enzyme was 0.81U/㎖ in smoker. Accodingly, there was no recognizabIe differences in the enzyme activity between smoker and non-smoker. 4. The human salivary superoxide dismutase was purified approximately 90 folds, and the molecular weight of the purified enzyme was estimated to be approximately 32,000 by gel filtretion. 4. Purified salivary superoxide dismutase has contained 1.83 atoms of copper and 2.00 atoms of zinc per molecule and was strikingly similar to the superoxide dismutase previonsly isolated from other cukaryotes by other investigatury. 5. The ultraviolet absorption spectrum of purified salivan- superoxide dismutase was 268㎚ which was similar to the value previously seen with the cytosolic enzyme of eukaryotes.
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배양 흰쥐 인슐린종세포에서 싸이클로스포린에 의한 세포독성에 미치는 글루타치온의 영향
최동헌,이병래,장대용,김종오,김병수,정기영,임태영,신병철,배학연 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.1
연구배경:Cycloporine A는 장기이식 환자에게 면역거부 반응을 억제할 목적으로 사용되고 있는데 이는 당뇨병, 고혈압, 신장세포 손상 등 여러 가지 부작용을 초래한다. Cyclosporine A의 이런 부작용에 대한 독성 기전은 아직 명확치 않으나 산화적 세포손상이 한 요인으로 생각되고 있다. 본 실험에서는 Cyclosprine A에 의한 췌장 베타세포의 독성이 미치는 GSH의 영향을 알아 보았다. 방법:흰쥐 인슐린종(RIN5mF)세포를 배양한 후 배양액에 글루타치온 합성효소 억제제인 buthione sulfoximine(BSO)과 cyclosporine A(CsA)및 GSH을 첨가하여 배양한 후 생존한 세포를 MTT법으로 측정하였다. 결과:CsA 용량이 증가함에 따라서 흰쥐 인슐린종 세포의 생존율은 감소되었다. BSO+CsA 투여군의 MTT 환원율은 CsA 단독 투여군보다 감소되었다. GSH+CsA 투여군의 MTT 환원율은 CsA 단독 투여군보다 증가되고, GSH+CsA+BSO 투여군의 MTT 환원율은 CsA+BSO 투여군보다 증가되었다. 결론:CsA는 췌장 베타세포에 직접적인 손상을 유발할 수 있으며, GSH은 CsA에 의한 베타손상방지 기능이 있는 것으로 추측된다. Background : Cyclosporin A (CsA) is a immunosuppressive agent that is most widely used in organ transplanted patients to prevent immunorejection, However, it has some side effects, including diabetes mellitus, nephrotoxicity and hypertension. The mechanism of CsA cytotoxicity is unclear but it has been suggested that reactive oxygen species are involved in the cytotoxic reactions. The purpose of this study was to determine the effects of glutathione, as a physiological antioxidant on CsA induced β-cell toxicity. Methods : Rat insulinoma (RINm5F) cells were incubated with culture media (RPMI1640) in the presence of CsA and/or buthionine sulfoximine (BSO), which is an inhibitor of r-glutamyl cysteine synthetase, and reduced glutathione. The viable cells were examined using 3-〔4,5-dimethylthiazol-2-yl〕-2,5-diphenyl tetrazolium bromide (MTT) and was determined by a spectrophotometer at a wavelength of 570 nm. Results : Incubating the RINm5F cells with CsA resulted in a decrease in cell viability with increasing dose. This deceased cells viability, induced by CsA was potentiated by BSO treatment. The CsA and BSO induced cells toxicity was reduced significantly by the reduced glutathione. Conclusion : The results suggest that pancreatic β-cell may be injured by CsA and glutathione may have some role in cytotoxicity (J Kor Diabetes Asso 26:57~63, 2002).
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