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자궁경부에서 인유두종 바이러스 검출을 위한 다중 중합효소 연쇄반응법 (Multiplex PCR)과 Hybrid Capture법의 비교 분석
한세준 ( Sei Jun Han ),김산 ( San Kim ),강민창 ( Min Chang Kang ),임주성 ( Ju Seong Lim ),안태규 ( Tae Gyu Ahn ),이병래 ( Byung Rai Lee ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.1
Objective : To compare the multiplex-PCR and hybrid capture methods for detection of Human papillomavirus (HPV) in uterine cervical swab samples. Methods : This study determined the HPV infection and its risk group (subtype) in uterine cervical swab sampl
배양 자궁경부암 세포주에서 Cis - platinum 의 세포독성에 대한 Glutatione 의 영향
안태규(Tae Gyu Ahn),한세준(Sei Jun Han) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.8
Objective : The purpose of this study is to determine the effects of glutathione on cisplatin-induced cytotoxicity of human cervical carcinoma cell lines (SiHa: squamous cell carcinoma cell, CaSki: epidermoid metastatic carcinoma cell). Methods : Human cervical carcinoma cells (SiHa, CaSki) were incubated with culture media (RPMI1640) in the presence of cisplatin and/or buthionine sulfoximine (BSO), as a inhibitor of 7-glutamylcysteine synthetase, and/or glutathione (GSH) and/or 2-oxo 4-thiazolidine carboxylic acid (OTC). The viable cells were examined by using 3-[4,5-dirnethylthiazol-2-yll-2,5-diphenyl tetrazolium bromide (MTT) and was determined by spectrophotometer at 570 nm. Results : The incubation of cervical cells with cisplatin resulted in an decreasing cells viability by dose response. The MTT reduction rate were not different by BSO (5 mM) treatment in cervical cell lines. The viable cells were increased significantly by glutathione (5 mM) or OTC (5 mM) in cisplatin-treated cell lines. Conclusion : y -glutamylcysteine synthetase inhibitor had no effect on cisplatin toxicity. GSH and OTC had effect on cisplatin cytotoxicity. So, These result suggested that cervical cancer line cells were more susceptive to protective effects of glutathione and OTC than BSO on cisplatin induced-toxicity.
김영혜 ( Young Hye Kim ),박준선 ( Jun Seon Park ),최상준 ( Sang Joon Choi ),송창훈 ( Chang Hoon Song ),한세준 ( Sei Jun Han ),정혁 ( Hyuk Jung ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.6
Objective: Burch operation has been effective method for the treatment of stress incontinence. Burch operation is a well-accepted procedure for treating stress urinary incontinence secondary to urethral hypermobility without intrinsic sphincter deficiency and is the reference standard with which other procedures are compared. This study is to evaluate the effectiveness of the Choi`s hook in laparoscopic Burch operation. Methods: This study included 47 patients who had undergone laparoscopic Burch operation from October 2000 through March 2005. Of these 47 patients, 27 patients underwent traditional laparoscopic Burch operation (Group 1) and 20 patients underwent laparoscopic Burch operation with Choi`s Hook on anchoring the endopelvic fascia to the Cooper`s ligament (Group 2). Results: The mean age of patients of Group 1 was 55.6±9.37 years of age and that of Group 2 was 56.0±5.93 years of age. There is no difference in the mean age of patients (P>0.05). The mean operating time was 90.5±15.32 minutes for Group 1 and 38.5±10.14 minute for Group 2. The mean operating time was shorter in Group 2 than Group 1 (P<0.05). There was no difference in recurrence rate and complication rate between Group 1 and Group 2 (P>0.05). Conclusion: We performed laparoscopic Burch operation with the Choi`s Hook on anchoring the endopelvic fascia to the Cooper`s ligament. When we compared traditional laparoscopic Burch operation with laparoscopic Burch operation with Choi`s Hook, we could shorten the operation time using the Choi`s Hook.
부인과 골반수술에 있어서 질식 접근 방법에 대한 임상적 시도
김태중 ( Tae Jung Kim ),임현정 ( Hyun Jung Im ),김산 ( San Kim ),한세준 ( Sei Jun Han ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.1
Objective : To analyse the clinical aspects of vaginal approach for gynecologic pelvic surgery, we evaluated age distribution, parity, indications for hysterectomy, postoperative pathology, operating time, bleeding amount, weight of uterus, postoperative
미산부 여성에서 발생한 초기 자궁 내막암의 고용량 Megestrol Acetate와 광역동 요법 (photodynamic therapy)을 이용한 치료
안태규 ( Tae Gyu Ahn ),강민창 ( Min Chang Kang ),박준선 ( Jun Sun Park ),조영신 ( Young Shin Cho ),최범채 ( Bum Chae Choi ),김동원 ( Dong Won Kim ),한세준 ( Sei Jun Han ),( Victor Sokolov ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.4
안태규 ( Tae Gyu Ahn ),정혁 ( Hyuk Jung ),송창훈 ( Chang Hun Song ),최상준 ( Sang Joon Choi ),이병래 ( Byoung Rai Lee ),한세준 ( Sei Jun Han ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.6
Objective : The aim of this article was investigated whether changes of superoxide dismutase isozymes in the placenta of patients with preeclampsia contribute to radical-induced tissue injury. Methods : The activities of antioxidant enzymes (superoxide di