加味慈桃丸 構成藥物의 血管新生 抑制敎果에 관한 硏究(Ⅱ)

왕덕중,박준혁,유영법,심범상,최승훈,안규석 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.2

We has proved the anti-metastatic and anti-angiogenic effects of the ingredients of the Jiaweicitaowan(JWCTW) and known about what ingredients are more effective. and had further experiments with that ingredients of JWCTW. We performed the following experiments and the results are listed as below: In capillary-like tube formation assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the dramatic inhibition effects on tube formation of ECV304 at the concentration of 40㎍/㎖. In ex vivo rat aortic ring assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the inhibition effects on angiogenesis of rat aorta at the concentration of 40㎍/㎖. Key word : Jiaweicitaowan(加味慈桃丸), cancer, angiogenesis

가미자도환(加味慈桃丸) 구성약물(構成藥物)의 혈관신생(血管新生) 억제효과(抑制效果)에 관한 연구(硏究)

왕덕중,강희,심범상,김성훈,최승훈,안규석,Wang, Teh-Chung,Kang, Hee,Shim, Bum-Sang,Kim, Sung-Hoon,Choi, Seung-Hoon,Ahn, Kyoo-Seok 대한암한의학회 2006 大韓癌韓醫學會誌 Vol.11 No.1

Jiaweicitaowan (JWCTW) has been used to inhibit recurrence and metastasis of cancer in clinical practice. Further study has shown its anti-metastatic and anti-angiogenic effects. By applying in vitro and in vivo anti-angiogenic evaluation model, the author assayed the each ingredients of JWCTW. The author performed the following experiments and the results are listed as below: Cell viability assay showed that the viability was almost identical between that of the control and that of the ingredients extract 40 ${\mu}g/m{\ell}$ treated, except of hexane fraction of Curcumae Radix (40 ${\mu}g/m{\ell}$, 2.0% of control), ethylacetate fraction (40 ${\mu}g/m{\ell}$, 26.7%), butanol fraction (20 ${\mu}g/m{\ell}$, 87.2%; 40 ${\mu}g/m{\ell}$, 12.5%) of Cremastrae appendiculatae Tuber, water fraction of Persicae Semen (40 ${\mu}g/m{\ell}$, 82.7%), ethylacetate fraction of Hippocampus (40 ${\mu}g/m{\ell}$, 85.3%). The results of gelatin zymogram assay showed that the ingredients of JWCTW decreases the gelatinolytic activity of MMP-9 from ECV304, at the concentration of 10 ${\mu}g/m{\ell}$. In in vitro invasion assay, the ingredients of JWCTW effectively inhibited the invasion of cancer cells as compared with the control (+PMA) groups. In capillary-like tube formation assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the dramatic inhibition effects on tube formation of ECV304 at the concentration of 40 ${\mu}g/m{\ell}$. In ex vivo rat aortic ring assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the inhibition effects on angiogenesis of rat aorta at the concentration of 40 ${\mu}g/m{\ell}$. According to the above research, the anti-angiogenic effects of the ingredients of JWCTW was proved and it suggested that the more effective prescription for anti angiogenesis could be developed.

加味慈桃丸 構成藥物의 血管新生 抑制效果에 관한 硏究Ⅰ

王德仲,朴俊赫,劉永法,沈範相,安圭錫,崔昇勳 대한동의병리학회 2000 동의생리병리학회지 Vol.14 No.2

angiogenesis 抑制 效果를 실험적으로 검증한 加味慈桃丸의 效能에 대한 硏究의 다음 단계로서, 韓藥物을 이용한 新藥 製材 開發의 전초단계로 본 실험을 시행하였다. 慈桃丸의 加味方으로 모두 6개의 藥物^1)로 構成된 加味慈桃丸를 ECV304 cell line을 利用하여 MTT assay에 의한 viability 測定, Gelatin zymogram assay, Invasion assay에 대한 實驗을 試行한 결과, 加味慈桃丸 構成藥物의 세포독성은 약물 A의 hexane (40 ㎍/㎖) 분획추출물, 약물 B의 ethyl acetate (40 ㎍/㎖)·butanol (20·40 ㎍/㎖) 분획추출물, 약물 C의 water (40 ㎍/㎖) 분획추출물, 약물 E의 ethylacetate (40 ㎍/㎖) 분획추출물이 대조군에 비해 세포생존율이 저하로 나타났으며, Gelatinolytic activity 억제기전에 미치는 영향에서 약물 A의 hexane 분획추출물은 20 ㎍/㎖·ethylacetate 분획추출물은 10·20·40 ㎍/㎖, 약물 B의 hexane 분획추출물은 10·20·40 ㎍/㎖·ethylacetate 분획추출물은 10·20㎍/㎖, 약물 C의 ethylacetate 분획추출물은 40 ㎍/㎖ 농도에서 MMP-9의 활성 저해 효과가 나타났다. Invasion 억제기전에 미치는 영향에서는 대조군의 50%이하로 감소한 것은 약물 A의 haxane 분획추출물 (35% of control+PMA), 약물 B의 water 분획추출물 (47%), 약물 C의 hexane 분획추출물 (30%)·ethylacetate 분획추출물 (29%)·butanol 분획추출물 (31%), 약물 D의 hexane 추출물 (34%)·butanol 분획추출물 (49%), 약물 F의 ethylacetate 분획추출물 (35%)로 나타났다. 이상이 實驗 結果를 통해 볼 때 加味慈桃丸의 angiogenese 抑制 效果는 많은 부분 약물 A, 약물 B, 약물 C의 haxane 및 ethylacetate 분획에서 나타남을 알 수 있으며, 특히 약물 A의 ethylacetate 추출물이 全 實驗을 통해 가장 강력한 效果를 나타내는 것으로 나타났다. Jiaweicitaowan (JWCTW) has been used to inhibit recurrence and metastasis of cancer in clinical practice. Further study has shown its anti-metastatic and anti-angiogenic effects. By applying in vitro and in vivo anti-angiogenic evaluation model, the author assayed the each ingredients of JWCTW. The author performed the following experiments and the results are listed as below; 1. Cell viability assay showed that the viability was almost identical between that of the control and that of the ingredients extract 40 ㎍/㎖ treated, except of hexane fraction of Curcumae Radix (40 ㎍/㎖, 2.0% of control), ethylacetate fraction (40 ㎍/㎖, 26.7%), butanol fraction (20 ㎍/㎖, 87.2%; 40 ㎍/㎖, 12.5%) of Cremastrae appendiculatae Tuber, water fraction of Persicae Semen (40 ㎍/㎖, 82.7%), ethylacetate fraction of Hippocampus (40 ㎍/㎖, 85.3%). 2, The results of gelatin zymogram assay showed that the ingredients of JWCTW decreases the gelatinolytic activity of MMP-9 from ECV304, at the concentration of 10 ㎍/㎖. 3. In in vitro invasion assay, the ingredients of JWCTW effectively inhibited the invasion of cancer cells as compared with the control (+PMA) groups. 4. In capillary-like tube formation assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the dramatic inhibition effects on tube formation of ECV304 at the concentration of 40 ㎍/㎖. 5. In ex vivo rat aortic ring assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrace appendiculatae Tuber and Persicae Semen showed the inhibition effects on angiogenesis of rat aorta at the concentration of 40 ㎍/㎖. According to the above research, the anti-angiogenic effects of the ingredients of JWCTW was proved and it suggested that the more effective prescription for anti angiogenesis could be developed.

어혈비로 변증한 Brown-Sequard Syndrome 치험 1예

왕덕중,정병주,장혜진,이지영,Wang, Teh-Chung,Jeong, Byeong-Ju,Jang, Hae-Jin,Lee, Ji-Young 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.2

Brown-Sequard syndrome is an incomplete spinal cord lesion characterized by ipsilateral weakness, loss of proprioceptive and vibratory sensation on the same side. and loss of pain and temperature sensation on the contralateral side. This clinical case was analysed on a patient with Brown-Sequard syndrome. The patient was admitted on January 26th, 2005. and departed on March 28th, 2005. He was treated as Eo-Hyeol Bee-Jeung. After treatment, His motor grade and weakness were improved and sensory ability about pain and temperature were regaine. We suggest that oriental medicine therapy is effective in treatment on Brown-Sequard syndrome.

가미자도환(加味慈桃丸) 구성약물(構成藥物)의 혈관신생(血管新生) 억제효과(抑制效果)에 관한 연구(硏究)

왕덕중,심범상,안규석,최승훈,Wang, Teh-Chung,Shim, Bum-Sang,Ahn, Kyoo-Seok,Choi, Seong-Hoon 대한암한의학회 2002 大韓癌韓醫學會誌 Vol.8 No.1

Jiaweicitaowan (JWCTW) has been used to inhibit recurrence and metastasis of cancer in clinical practice, Further study has shown its anti-metastatic and anti-angiogenic effects. By applying in vitro and in vivo anti-angiogenic evaluation model, the author assayed the each ingredients of JWCTW. The author performed the following experiments and the results are listed as below: 1. Cell viability assay showed that the viability was almost identical between that of the control and that of the ingredients extract $40{\mu}g/ml$ treated, except of hexane fraction of Curcumae Radix $(40{\mu}/ml,\;2.0\%\;of\;control)$, ethyl acetate fraction $(40{\mu}g/ml,\;26.7%)$, butanol fraction $(20{\mu}g/ml,\;87.2%;\;40{\mu}g/ml,\;12.5%)$ of Cremastrae appendiculatae Tuber, water fraction of Persicae Semen $(40{\mu}g/ml,\;82.7%)$, ethylacetate fraction of Hippocampus $(40{\mu}g/ml,\;85.3%)$. 2. The results of gelatin zymogram assay showed that the ingredients of JWCTW decreases the gelatinolytic activity of MMP-9 from ECV304, at the concentration of $10{\mu}g/ml$. 3, In in vitro invasion assay, the ingredients of JWCTW effectively inhibited the invasion of cancer cells as compared with the control (+PMA) groups. 4. In capillary-like tube formation assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the dramatic inhibition effects on tube formation of ECV304 at the concentration of $40{\mu}g/ml$. 5. In ex vivo rat aortic ring assay, the hexane and ethyl acetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the inhibition effects on angiogenesis of rat aorta at the concentration of $40{\mu}g/ml$. According to the above research, the anti-angiogenic effects of the ingredients of JWCTW was proved and it suggested that the more effective prescription for anti angiogensis could be developed.

수면장애를 동반한 당뇨병성 소양증 환자에 대한 육미지황탕가미방(六味地黃湯加味方) 투여 1례

왕덕중,김광호,김진우,이순이,정병주,장혜진,이지영,Wang, Teh-Chung,Kim, Kqang-Ho,Kim, Jin-Woo,Lee, Soon-Yee,Jeong, Byeong-Ju,Jang, Hae-Jin,Lee, Ji-Young 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.3

This study was designed to evaluate the effects of oriental medicine therapy on insomnia and diabetic pruritus. This clinical case was analysed on a patient with diabetus mellitus whose main symptom was sleep disorder, which was attributed to pruritus. The patient was admitted on July 16th, 2004, and departed on September 3rd, 2004. He was treated internally and externally with herbal medicine, acupuncture, and bloodletting therapy. After treatment, pruritus and sleep disorder decreased. The study suggests that orental medicime therapy is effective in treatment of diabetic pruritus accompanied by insomnia.

계류유산 후 발생한 뇌졸중 환자에 대한 임상적 고찰

이순이,이철웅,왕덕중,이지영,김진우 대한한방부인과학회 2005 大韓韓方婦人科學會誌 Vol.18 No.2

Purpose : The purpose of this study is to report the effect of oriental and western medicine combination therapy for the postpartum stroke after missed abrotion. Methods : The patient in this case, 31-years-old female, was admitted into the Joong-hwa oriental hospital. The symptoms of patient in this case are right hemi paresis, aphasia and dysphagia. This patient have treated with oriental and western medicine combination treatments such as herb medication, acupuncture, manual therapy, moax treatment, and so forth. Results : After oriental and western medicine combination therapy, the patient have improved in all disturbance of the postpartum stroke after missed abortion. Conclusion : The postpartum stroke after missed abortion would be treated more carefully in oriental medicine considering women's well-being.

중풍 편마비 환자의 손 기능 회복에 대한 八邪穴 刺鍼의 임상적 효과

김자영,정선미,박찬규,민은경,왕덕중 대한침구의학회 2008 대한침구의학회지 Vol.25 No.5

Objectives : Investigate the effectiveness of an acupuncture at Palsa(BaXie) in hemiparetic patients after stroke. Methods : Acupuncture at Palsa(BaXie) applied to thirty one hemiparetic patients who had been by general treatment after stroke. The other thirty one hemiparetic patients had only general treatment. Outcomes were assessed by Medical research council scale motor grade(MRCSMG) of wrist, grip power, Fugl-Meyer Motor Scale(FMS) of hand, and Motricity Index(MI) of fingers. Results : The patients exhibited substantial improvements on grip power and Fugl-Meyer Motor Scale of hand, but Medical research council scale motor grade of wrist and Motricity Index of fingers have not significantly meaningful differences between sample and control group. Conclusions : Acupuncture at Palsa(BaXie) may be an effective method of improving hand function of hemiparetic patients after stroke.

火病을 紫河車 藥鍼을 使用하여 治療한 1例

김진우,강형원,안대종,이순이,왕덕중,이지영 대한한방신경정신과학회 2005 동의신경정신과학회지 Vol.16 No.1

Hwa-byeong, the syndrome usually found in Korean society, is a culture-relateddisease. The common symptoms of Hwa-byeong are flushed face with fever, chest discomfort, palpitation, insomnia, headache, dizziness, and dyspepsia.In this case, we diagnosed Hwa-byeong by HBDIS(Hwa-Byeong Diagnostic Interview(Schedule) and treated the patient with Jahageo Herbal acupuncture at Shindo(Shendao)Yongdae(Lingtai), Chiyang(Zhiyang), Kumi(Jiuwei) and Chonjung(Shanzhong). After that, her subjective symptoms improved.With this result, It was considered that Jahageo Herbal acupuncture was very effective for treatment of Hwa-byeong.

불면증에 이침 시술을 한 환자 20례 임상고찰

김진우,강형원,안대종,왕덕중,이순이 대한한방신경정신과학회 2004 동의신경정신과학회지 Vol.15 No.1

OBJECTIVE: This study is aimed to evaluate the clinical improvement of insomnia and patients' original disease after the therapy of auricular acupuncture. METHODS: A questionnaire regarding patients' sleeping behavior was executed to secure the objectivity. 20 patients were selected from 55 patients in Joong Hwa oriental medicine hospital. 20 patients who had undergone auricular acupuncture for insomnia for 6 days were asked before the first therapy to fill in a form regarding their sleeping behavior and retrospectively after 6 days a questionnaire regarding the success of therapy. Auricular acupuncture therapy consisted of 3 consultations at an interval of 2 days. RESULTS: 12 patients were good conditions, 5 were same conditions and 3 were rather poor conditions of 20 patients after auricular acupuncture therapy. CONCLUSIONS: 60% of object improved insomnia after auricular acupuncture therapy. Auricular acupuncture therapy can be applied and adapted individually, furthermore it is economical and small side effects.