밝기가 다른 이미지에서의 레퍼런스 이미지 결정 방법

오창재(Changjae Oh),함범섭(Bumsub Ham),신형철(Hyungchul Shin),손광훈(Kwanghoon Sohn) 한국방송·미디어공학회 2011 한국방송공학회 학술발표대회 논문집 Vol.2011 No.7

컬러는 영상처리 분야에서 중요한 단서로 사용될 수 있는 정보이다. 하지만 실제로 촬영한 영상의 경우에는 빛과 카메라 특성 등 다양한 요소들의 영향으로 인해 이미지 간 컬러 정보의 불일치가 빈번히 일어난다. 따라서 컬러가 다른 여러 장의 영상을 입력 영상으로 사용하는 경우, 입력 영상간 컬러를 동일하게 맞춰 주어야 한다. 이를 수행함에 있어서, 어떠한 이미지를 레퍼런스 이미지로 결정할 것인가는 매우 중요한 문제이다. 이에 본 논문에서는, 히스토그램 등화(histogram equalization) 기법을 이용하여 입력 이미지들의 비용을 결정해줌으로써, 레퍼런스 이미지를 결정하는 방법을 제시한다. 스테레오 매칭을 통해 다양한 밝기의 입력 영상에서 가장 좋은 결과를 얻을 수 있는 레퍼런스 이미지를 결정할 수 있음을 보였다.

GmREM1.1 and GmREM2.1, which encode the remorin proteins in soybean, have distinct roles during root nodule development

손승민,오창재,배주희,이형석,안정선 한국식물학회 2015 Journal of Plant Biology Vol.58 No.1

Remorin is involved in various biotic and abiotic stresses, including host-microbe interactions. To elucidate its roles during root nodule development, we isolated and characterized five remorin genes in soybean. The genes belonging to groups 1 and 2 were both expressed during root nodule development but with somewhat different temporal patterns. Using a biochemical assay, we showed that the homo-oligomerization of GmREM1.1 was mediated by the coiled-coil region in the C-terminal domain in combination with either the N-terminal or C-terminal anchor peptides (RemCA). Plasma membrane targeting was also sufficiently mediated by RemCA from GmREM1s but not from GmREM2.1. In addition, GmREM1.1 was highly expressed in the nodule primordia and inner cortex region of root nodules, whereas GmREM2.1 transcription was mainly detected in the infected cells. Moreover, RNAi-GmREM2.1 hairy roots showed significantly reduced nodule formation, but RNAi-GmREM1.1 had little effect on nodule formation. Taken together, our results suggest that GmREM1.1 and GmREM2.1, with different molecular features, function distinctively during root nodule development.

Molecular Cloning and Complementation Analysis of nifV Gene from Frankia EuIK1 Strain

안정선,오창재,김호방 한국분자세포생물학회 2003 Molecules and cells Vol.15 No.1

Arabidopsis thaliana Remorins Interact with SnRK1 and Play a Role in Susceptibility to Beet Curly Top Virus and Beet Severe Curly Top Virus

손승민,오창재,안정선 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.3

Remorins, a family of plant-specific proteins containing a variable N-terminal region and conserved C-terminal domain, play a role in various biotic and abiotic stresses,including host-microbe interactions. However, their functions remain to be completely elucidated, especially for the Arabidopsis thaliana remorin group 4 (AtREM4). To elucidate the role of remorins in Arabidopsis, we first showed that AtREM4s have typical molecular characteristics of the remorins, such as induction by various types of biotic and abiotic stresses, localization in plasma membrane and homo- and hetero-oligomeric interaction. Next, we showed that their loss-of-function mutants displayed reduced susceptibility to geminiviruses,Beet Curly Top Virus and Beet Severe Curly Top Virus, while overexpressors enhanced susceptibility. Moreover, we found that they interacted with SnRK1, which phosphorylated AtREM4.1, and were degraded by the 26S proteasome pathway. These results suggest that AtREM4s may be involved in the SnRK1-mediated signaling pathway and play a role as positive regulators of the cell cycle during geminivirus infection.

감귤 분자육종을 위한 분자표지 개발 현황 및 전망

김호방,김재준,오창재,윤수현,송관정,Kim, Ho Bang,Kim, Jae Joon,Oh, Chang Jae,Yun, Su-Hyun,Song, Kwan Jeong 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.3

세계적인 과수작물로서의 경제적 중요성에도 불구하고, 감귤 생산은 주로 자연교잡 실생이나 눈 돌연변이로부터의 선발 또는 단순 품종 도입 등을 통해 이루어지고 있는 실정이다. 긴 유년기, 다배성, 자가불화합성과 같은 감귤 고유의 식물학적 특성, 주요 형질들(병저항성, 수량성, 품질 등)의 QTL에 의한 조절 등은 전통 육종을 통한 우수 품종의 개발을 어렵게 하는 요인이다. 지구 온난화에 의한 생산 여건의 급격한 변화, 소비자 요구 다양화 등은 고품질 감귤의 조기 선발과 안정적 생산, 품종 다양화, 육종 비용 절감 등을 위한 체계적인 감귤 분자육종 프로그램의 도입을 요구하고 있다. 동위효소를 이용한 최초의 감귤 연관지도 작성이 이루어진 이래, 다양한 분자표지를 이용한 연관지도 작성, 생물(CTV, CiLV, ABS, 선충] 및 비생물적(염분, 저온) 스트레스, 아포믹시스, 다배성, 과실착색(카로티노이드, 안토시아닌), 무종자, 웅성불임, 신맛 적음, 생식, 형태(나무, 잎, 꽃, 열매 등), 과실 품질, 종자수, 수량성, 조기 착과 등과 연관된 분자표지 발굴, QTL 맵핑 등이 이루어졌다. CTV 저항성과 적육(안토시아닌 축적) 형질에 대해서는 유전자 클로닝이 이루어졌고, 교배 육종 효율 증대 및 비용 절감을 위해 교잡배와 주심배를 구분하기 위한 다수의 simple sequence repeat (SSR) 분자표지가 개발되었다. 최근, 스위트오렌지와 '클레멘타인' 만다린에 대한 고품질의 표준 유전체가 완성되어 유전체 기반 감귤 분자육종을 위한 토대가 마련되었다. 표준 유전체 정보를 토대로 대규모 분자표지(SNP, SSR, InDel) 기반의 표준 연관 및 물리지도 작성, 비교 유전체 지도 작성, gene annotation, 전사체 분석 등이 활발히 이루어지고 있다. 감귤 유전자원 및 핵심집단에 대해 표준 유전체 기반 비교 유전체 분석, GBS (genotyping-by-sequencing), GWAS (genome wide association study) 등을 통해 감귤의 다양한 형질과 연관된 분자마커 발굴 및 개발, 유용/변이 유전자 클로닝 등에 관한 연구가 가속화될 것으로 전망된다. 또한 표적 유전체 교정 및 VIGS (virus-induced gene silencing) 기술도 유전자 마커의 검증을 비롯한 감귤 분자육종 프로그램에 활발히 이용될 것이다. Citrus is an economically important fruit crop widely growing worldwide. However, citrus production largely depends on natural hybrid selection and bud sport mutation. Unique botanical features including long juvenility, polyembryony, and QTL that controls major agronomic traits can hinder the development of superior variety by conventional breeding. Diverse factors including drastic changes of citrus production environment due to global warming and changes in market trends require systematic molecular breeding program for early selection of elite candidates with target traits, sustainable production of high quality fruits, cultivar diversification, and cost-effective breeding. Since the construction of the first genetic linkage map using isozymes, citrus scientists have constructed linkage maps using various DNA-based markers and developed molecular markers related to biotic and abiotic stresses, polyembryony, fruit coloration, seedlessness, male sterility, acidless, morphology, fruit quality, seed number, yield, early fruit setting traits, and QTL mapping on genetic maps. Genes closely related to CTV resistance and flesh color have been cloned. SSR markers for identifying zygotic and nucellar individuals will contribute to cost-effective breeding. The two high quality citrus reference genomes recently released are being efficiently used for genomics-based molecular breeding such as construction of reference linkage/physical maps and comparative genome mapping. In the near future, the development of DNA molecular markers tightly linked to various agronomic traits and the cloning of useful and/or variant genes will be accelerated through comparative genome analysis using citrus core collection and genome-wide approaches such as genotyping-by-sequencing and genome wide association study.

Comparison of Repositioning Error According to Eccentric and Concentric Contraction of the Ankle Dorsiflexor Muscle in the Ankle Joint

오진희,김주상,오창재,이미영 대한물리치료학회 2023 대한물리치료학회지 Vol.35 No.2

Purpose: This study compared the movement control ability of the ankle joint according to the type of muscle contraction, namely, eccentric or concentric contractions. Methods: Thirty-four healthy adult subjects participated in this study. As a single group, before the experiment, the subjects were trained on achieving the required position of the ankle around the target point by manually controlling the ankle dorsiflexion by 10°. Concentric contraction starts at 0° and continues until the target point of 10° is reached. During an eccentric contraction, the ankle joint starts at 20° ankle dorsiflexion and continues till the target point is reached. Movements using eccentric contraction and concentric contraction were randomly performed 3 times each. Results: The results of comparing the difference in the movement control ability of each type of muscle contraction of ankle dorsiflexion showed that the measurement-remeasurement error was significant in eccentric contraction. Conclusion: In this study, we found a difference in the ability to control movement according to whether the contraction is eccentric or concentric. Therefore, we propose that the ability to control movement is affected by the type of muscle contraction.

감귤 분자육종을 위한 분자표지 개발 현황 및 전망

김호방,김재준,오창재,윤수현,송관정 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.3

Citrus is an economically important fruit crop widely growing worldwide. However, citrus production largely depends on natural hybrid selection and bud sport mutation. Unique botanical features including long juvenility, polyembryony, and QTL that controls major agronomic traits can hinder the development of superior variety by conventional breeding. Diverse factors including drastic changes of citrus production environment due to global warming and changes in market trends require systematic molecular breeding program for early selection of elite candidates with target traits, sustainable production of high quality fruits, cultivar diversification, and cost-effective breeding. Since the construction of the first genetic linkage map using isozymes, citrus scientists have constructed linkage maps using various DNA-based markers and developed molecular markers related to biotic and abiotic stresses, polyembryony, fruit coloration, seedlessness, male sterility, acidless, morphology, fruit quality, seed number, yield, early fruit setting traits, and QTL mapping on genetic maps. Genes closely related to CTV resistance and flesh color have been cloned. SSR markers for identifying zygotic and nucellar individuals will contribute to cost-effective breeding. The two high quality citrus reference genomes recently released are being efficiently used for genomics-based molecular breeding such as construction of reference linkage/ physical maps and comparative genome mapping. In the near future, the development of DNA molecular markers tightly linked to various agronomic traits and the cloning of useful and/or variant genes will be accelerated through comparative genome analysis using citrus core collection and genome-wide approaches such as genotyping-by-sequencing and genome wide association study. 세계적인 과수작물로서의 경제적 중요성에도 불구하고, 감귤 생산은 주로 자연교잡 실생이나 눈 돌연변이로부터의선발 또는 단순 품종 도입 등을 통해 이루어지고 있는 실정이다. 긴 유년기, 다배성, 자가불화합성과 같은 감귤 고유의식물학적 특성, 주요 형질들(병저항성, 수량성, 품질 등)의QTL에 의한 조절 등은 전통 육종을 통한 우수 품종의 개발을 어렵게 하는 요인이다. 지구 온난화에 의한 생산 여건의급격한 변화, 소비자 요구 다양화 등은 고품질 감귤의 조기선발과 안정적 생산, 품종 다양화, 육종 비용 절감 등을 위한체계적인 감귤 분자육종 프로그램의 도입을 요구하고 있다. 동위효소를 이용한 최초의 감귤 연관지도 작성이 이루어진 이래, 다양한 분자표지를 이용한 연관지도 작성, 생물(CTV, CiLV, ABS, 선충] 및 비생물적(염분, 저온) 스트레스, 아포믹시스, 다배성, 과실착색(카로티노이드, 안토시아닌), 무종자, 웅성불임, 신맛 적음, 생식, 형태(나무, 잎, 꽃, 열매등), 과실 품질, 종자수, 수량성, 조기 착과 등과 연관된 분자표지 발굴, QTL 맵핑 등이 이루어졌다. CTV 저항성과 적육(안토시아닌 축적) 형질에 대해서는 유전자 클로닝이 이루어졌고, 교배 육종 효율 증대 및 비용 절감을 위해 교잡배와주심배를 구분하기 위한 다수의 simple sequence repeat (SSR) 분자표지가 개발되었다. 최근, 스위트오렌지와 ‘클레멘타인’ 만다린에 대한 고품질의 표준 유전체가 완성되어 유전체 기반 감귤 분자육종을 위한 토대가 마련되었다. 표준 유전체 정보를 토대로 대규모 분자표지(SNP, SSR, InDel) 기반의 표준 연관 및 물리지도 작성, 비교 유전체 지도 작성, gene annotation, 전사체 분석 등이 활발히 이루어지고 있다. 감귤유전자원 및 핵심집단에 대해 표준 유전체 기반 비교 유전체 분석, GBS (genotyping-by-sequencing), GWAS (genome wide association study) 등을 통해 감귤의 다양한 형질과 연관된 분자마커 발굴 및 개발, 유용/변이 유전자 클로닝 등에 관한 연구가 가속화될 것으로 전망된다. 또한 표적 유전체 교정 및 VIGS (virus-induced gene silencing) 기술도 유전자 마커의 검증을 비롯한 감귤 분자육종 프로그램에 활발히 이용될 것이다.

Analysis of the Root Nodule-enhanced Transcriptome in Soybean

안정선,Cheol-Goo Hur,오창재,김호방,Sun-Yong Park 한국분자세포생물학회 2004 Molecules and cells Vol.18 No.1

For high throughput screening of root nodule-enhanced genes, cDNA libraries specific for three different developmental stages of soybean root nodules were constructed after inoculation with Bradyrhizobium japonicum USDA110. 5,469 cDNA clones were sequenced and grouped into 2,511 non-redundant (nr) ESTs consisting of 769 contigs and 1,742 singletons. Using similarity searches against several public databases we constructed a functional classification of the ESTs into root nodule-specific nodulin genes, stress-responsive genes and genes related to carbon and nitrogen metabolism. We also constructed a cDNA microarray with 382 selected clones that appeared to be up-regulated in the root nodule. Using the microarray we compared the transcript levels of uninfected roots and root nodules from four developmental stages. We identified 81 genes that were differentially expressed, and grouped them into seven clusters according to the similarity of their expression profiles, using a hierarchical clustering algorithm. Clusters 1, 2, 3, and 6, comprised of 58 genes, showed root nodule-enhanced expression. The information from this study will be used to analyze the roles of root nodule-specific genes and signaling pathways during root nodule development.

패널티 방법을 이용한 고속 경계 보존 평활화 기법

정형주(Hyeongju Jeong),오창재(Changjae Oh),김영중(Youngjung Kim),김선옥(Sunok Kim),손광훈(Kwanghoon Sohn) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6

In this paper, we propose an edge-preserving smoothing operator based on weighted absolute deviation. This formulation makes the algorithm robust for usage on noisy and textured image. Furthermore, we present an efficient technique to solve the proposed objective function. Specifically, we approximate the solution of nonlinear system, by iteratively solving a sequence of two subproblem. Such decomposition not only reduces the overall computation complexity but also enhances the results, comparing with conventional gradient based method.

KiwiPME1 Encoding Pectin Methylesterase Is Specifically Expressed in the Pollen of a Dioecious Plant Species, Kiwifruit (Actinidia chinensis)

김성철,엄윤경,고승희,오창재,곽용범,김홍림,이이,안창선,박훤범,김호방 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.3

Pectin methylesterases (PMEs) mediate demethylesterification of pectic polysaccharides such as homogalacturonan, a major component of the primary plant cell wall, in the apoplasm. The PMEs are implicated in a number of developmental processes, including pollen development and pollen tube growth, through the fine tuning of the methylesterification status of pectin. In this study, we isolated a full-length cDNA (KiwiPME1) encoding PME from kiwifruit and characterized its molecular features. Analyses of the primary protein structure and gene structure revealed that KiwiPME1 encodes a pre-pro-PME protein that is predicted to localize to the outside of the cell and belongs to group 2 (formerly type 1). The KiwiPME1 expression was highly detected in pollen grains of kiwifruit but not in vegetative tissues investigated. Expression pattern analysis of KiwiPME1 among different floral tissues of male and female plants revealed that KiwiPME1 was expressed specifically in the stamens of flower buds in male and female plants, whereas its expression was detected only in the stamens of male plants when the flowers opened. Expression analysis of KiwiPME1 promoter fused to the GUS reporter gene in Arabidopsis displayed a very similar pattern to that in kiwifruit. Our study suggests that the cell wall-localized KiwiPME1 is likely implicated in the pollen development and pollen tube growth of a dioecious species kiwifruit.