간세포암과 간경변증의 감별 진단에 있어서 Lens culinaris agglutinin-A 에 반응하는 AFP 분획 측정의 임상적 의의

송근암(Geum A m Song),김태오(Tae Oh Kim),송철수(Chul Soo Song),조몽(Mong Cho),양웅석(Ung Suk Yang),류수형(Soo Hyung Ryu),이성훈(Seong Hwun Lee),조병만(Byung Mann Cho) 대한내과학회 1999 대한내과학회지 Vol.57 No.5

N/A AFP has been the most useful tumor marker of hepatocellular carcinoma (HCC) because AFP is tumor specific and organ specific. However it can be elevated in liver cirrhosis (LC) and chronic hepatitis as well. AFP in hepatocellular carcinoma has been known to be highly reactive Lens Culinaris Agglutinin-A (LCA-A). The aim of our study is to find whether LCA-A reactive AFP (AFP-L3) is useful in differentiation of HCC and LC with high serum AFP level (>20 ng/ml). Methods : The material consists of sera from 18 patients with HCC and 16 patients with LC whose serum AFP concentration was above 20 ng/ml. Sera were tested for AFP-L3 by lectin-affinity electrophoresis, coupled with antibody affinity blotting with alpha-fetoprotein differentiation kit L (Wako Pure Chemical Industries, Osaka, Japan). AFP-L3 fraction percentage was checked by densitometry (580nm, Cliniscan II, Helena). Results : AFP-L3 detection rate in membrane was 61.1% in HCC and 37.5% in LC. When cut-off value level is 15%, the positive rate was 61.1% in HCC and 31.2% in LC, and sensitivity and specificity of AFP-L3 fraction in HCC was 61.1% and 68.8% respectively. In according to these results, AFP-L3 was a useful marker in differentiation HCC and LC with high serum AFP level. There was no significant relation in AFP-L3 fraction and tumor size, but there was significant relation between AFP-L3 fraction and total AFP serum level(P=0.049). Conclusion : These results lead us to conclude that the level of AFP-L3 is a useful marker in differentiation of HCC and LC with high serum AFP level. (Korean. J. Med 57:875-880, 1999)

상부 위장관 질환에서 IL-1β 및 IL-1RN 유전자 다형성

이준홍 ( Joon Hong Lee ),김광하 ( Gwang Ha Kim ),허정 ( Jeong Heo ),박도윤 ( Do Youn Park ),전은숙 ( Eun Sook Jun ),강대환 ( Dae Hwan Kang ),송근암 ( Geum Am Song ),조몽 ( Mong Cho ),양웅석 ( Ung Suk Yang ) 대한내과학회 2003 대한내과학회지 Vol.65 No.1

목적 : H. pylori 감염은 위,십이지장궤야, 위암 등 여러 종류의 상부 위장관 질환과 연관이 있다고 알려져 있으나, 일부의 감염자들에서만 병변을 일으킨다. 일한 H. pylori의 다양하고 예측하기 어려운 병인을 주로 세균의 병독성의 차이로 설명하려는 시도가 활발히 이루어져 왔으나 아직 확실한 결과를 얻지 못하고 있는 실정이다. 최근 연구에 의하면 IL-1β및 IL-1RN 유전자 다형성은 위산 분비, 장형화생, 만성 위축성 위염과 위암의 발생과 Background : IL-1 β and IL-1 receptor antagonist (IL-1RN) genetic polymorphisms have been associated with development of gastric atrophy and increased risk of gastric carcinoma. This study aimed to determine the effects of these polymorphisms in gastroduo

위암에서 Helicobacter pylori cagA 유전자와 숙주 Interleukin-1 Beta 및 Interleukin-1 수용체 길항제 유전자 다형성

이재승 ( Jae Seung Lee ),김광하 ( Gwang Ha Kim ),허정 ( Jeong Heo ),박도윤 ( Do Youn Park ),전은숙 ( Eun Sook Jun ),강대환 ( Dae Hwan Kang ),송근암 ( Geum Am Song ),조몽 ( Mong Cho ),양웅석 ( Ung Suk Yang ) 대한소화기학회 2003 대한소화기학회지 Vol.41 No.6

Background/Aims: Both Helicobacter pylori (H. pylori ) cagA genotype and host nterleukin-1 beta/interleukin-1 receptor antagonist (IL-1B/IL-1RN ) gene polymorphisms play a role in determining the clinical consequences of H. pylori infection. In this study, we investigated H. pylori cagA genotype and host IL-1B /IL-1RN polymorphisms in Korean patients to clarify their involvement in the occurrence of gastric carcinoma. Methods: The population comprised of 134 patients with H. pylori infection: 82 with gastric carcinoma and 52 with gastritis. The DNA was isolated from gastric biopsy sample and H. pylori cagA genotype was determined by PCR. The IL-1B ?11 polymorphism was genotyped by PCR-RFLP and the IL-1RN polymorphism was analyzed. Results: Infection with cagA+ H. pylori was not associated with an increased risk for gastric carcinoma. The IL-1B -511 *T carriers and IL-1RN *2 carriers did not show an increased risk for gastric carcinoma. Combining bacterial/host genotypes, cagA+ /IL-1B -511 *T carriers and cagA+ /IL-1RN *2 carriers showed no increased risk of gastric carcinoma. Classifying of gastric carcinoma into intestinal and diffuse type, the bacterial/host genotypes were also not associated with increased risk of each type. Conclusions: Combined H. pylori cagA gene and host IL-1B /IL-1RN polymorphisms showed no increased risk of gastric carcinoma. Therefore, it seems other endogenous or exogenous factors might play more important roles in the development of gastric carcinoma in Korean. (Korean J Gastroenterol 2003;41:438-446)

측방발육형 대장 종양의 임상-병리학적 특징 및 내시경적 치료 성적 비교

송근암,김동욱 부산대학교 병원 암연구소 2008 부산대병원학술지 Vol.- No.24

Purpose: Among colorectal neoplasms, laterally spreading tumors (LSTs) are defined as tumors over 10mm in diameter that are low in height and grow superficially. We analyzed the clinicopathologic characteristics of LSTs in colorectum and the outcomes of endoscopic treatments for LSTs. Subject and Methods: One hundred twenty one LSTs were analyzed according to their endoscopic and pathologic findings. Results: The most frequent location was asceding colon(41.3%), followed by rectum(24.8%). Morphologic types were GH types 28.0%(34/121), MN types 33.1% (41/121), FL types 19.8% (24/121), and PD types 18.1% (34/121). Sixty eight LSTs (56.2%) were less than 20 mm in diameter, 28(23.1%) were 20~30 mm, 14 (11.6%) were 30-40mm, and 11 (9.1%) were larger than 40 mm. The overall malignancy rate was 19.8% (24/121). Malignancy rates were 16.7% (16/96) in the lesions less than 30 mm, and 32% (8/25) in larger than 30 rom. Malignancy rates were 2.9% (1/34) in GH type, 31.7%(3/41) in MN type, 8.3% (2/24) in FL type, and 36.3%(8/22) in PD type. Conclusions: LSTs larger than 30 rom had high malignant potential. Furthermore, LSTs showed different clinicopathologic characteristics depending on the morphologic classification. MN or PD type had higher malignant potential than GH or FL type.

부산 경남지역의 Gas Chromatography 와 Mass Spectrophotometer(GC/MS)를 이용한 담낭담석 분석

김상현,강대환,강영진,양웅석,조몽,송철수,송근암,배상문 대한내과학회 1999 대한내과학회지 Vol.56 No.1

Objectives : The composition of gallstones is very variable depending on many factors such as diet, sex, race, genetic background, and so on. The compositional changes of gallstones have been reported in Korean people. Author studied the compositional characteristics of gallstones in Pusan area to see geographical difference. Material and Methods : Gas chromatography and Mass spectrophotometry was performed on the gallstones obtained from the 34 patients who had been taken cholecystectomy because of acute or chronic cholecystitis. Cholesterol gallstone was defined as the composition of the cholesterol is more than 50%. Results : The cholesterol gallstone was 67.6% (23/34) in the 34 patients. There was sexual difference in the cholesterol gallstone group, which the ratio of male and female was 1:2.3(7/16), while there was no difference in the non-cholesterol stone(5:6). The cholesterol stone consisted with cholesterol, bilirubin, bile acid, and palmitic acid and the propotion of them was 75.6%, 4.6%, 15.0%, and 1.8% respectively. While the proportion of non-cholesterol stone was 30.2%, 26.8%, 37.5%, and 5.5% respectively. Conclusions : The cholesterol stone was major fraction of gallstone of the patients in Pusan area. There was no difference on the compostion of gallstones between those of patients in Pusan area and in the other area of Korea.

위염과 위암 환자의 위점막 조직에서 cagA 양성 Helicobacter pylori 감염과 세포증식의 관계

김태오,강대환,박승근,양웅석,조몽,송철수,김형욱,송근암,김양정 대한내과학회 1999 대한내과학회지 Vol.57 No.2

Background: It has been suggested that cytotoxin associated gene A (cagA) is a marker of more virulent strains of H. pylori and cagA bearing H. pylori is more pathogenic in the gastrointestinal diseases. On the other hand, according to several reports H. pylori causes the cell proliferation, which may be an important mechanism of gastric carcinogenesis. So, we studied to elucidate whether there is the association of the cagA positive H. pylori infection with cell proliferation on the gastric mucosae of the patients with gastritis and gastric cancer or not. Methods: In this study, 27 gastritis and 35 gastric cancer patients were included. PCR assay for the detection of H. pylori(ureA PCR) and cagA bearing H. pylori(eagA PCR) were performed on the gastric mucosal biopsy specimen. Immunohistochemical study using the MIB 1 Ab against Ki 67 antigen was carried out to evaluate the cell proliferation. Results : The prevalence of H. pylori infection was 85.2%(23/27) in the patients with gastritis and 54.3%(19/ 35) in the patients with gastric cancer. The prevalence of cagA+ strain of H. pylori was 52.2%(12/23) and 47.4%(9/ 19) in the patients with gastritis and gastric cancer. In the patients with gastritis, the degree of cell proliferation was not different in the ureA positive(24.8%) and ureA negative(21.7%) gastric mucosae. Moreover, the difference of cell proliferation was not observed according to the presence or absence of cagA gene(29.4% vs 19.9%) among the ureA positive gastric mucosae. In the patients with gastric cancer, cell proliferation indices were 25.5% and 27.5% in the ureA postive and cagA negative gastric mucosae, 28.1% and 22.2% in the cagA positive and cagA negative group among the ureA positive gastric mucosae. There was no significant difference statistically. Conclusions : There was no association of cagA+ strain of H. pylori with cell proliferation in the gastric mucosae of the patients with gastritis and gastric cancer. It was presumed that more studies are needed to elucidate the role of H. pylori infection in the gastric carcinogenesis.