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DNA 직접추출법에 따른 산림토양 부식층 내 세균군집의 계통학적 다양성 비교
손희성,한송이,황경숙,Son, Hee-Seong,Han, Song-Ih,Whang, Kyung-Sook 한국미생물학회 2007 미생물학회지 Vol.43 No.3
개량된 manual법과 ISOIL kit를 이용하여 산림토양의 부식층 토양시료로부터 추출한 DNA를 대상으로 16S rDNA PCR 증폭산물을 cloning하고 구축된 clone에 대해 ARDRA cluster분식을 수행한 결과, 개량된 manual법에 의해 구축된 136 clones은 45개 ARDRA cluster로, ISOIL kit를 이용한 경우 충 76 clones은 44개 ARDRA cluster로 분류되었다. 각clone cluster로부터 대표 clone을 선발하여 16S rDNA염기서열을 결정한 결과, ISOIL kit의 경우 44개 대표clone은 ${\alpha}-,\;{\beta}-,\;{\gamma}-,\;{\delta}-Proteobacteria$, Acidobacteria 및 Actinobacteria의 3개 phylum계통군이 확인되었으며, 개량된 manual법에 의한 45개 대표 clone은 ${\alpha}-,\;{\beta}-,\;{\gamma}-,\;{\delta}-Proteobacteria$, Acidobacteria, Bacteroides, Verrucomicrobia, Planctomycetes, 그리고 Gemmatomonadetes의 충 6개 phylum의 다양한 계통군이 검출되었다. 이상의 결과로부터 개량된 manual법에 의래 추출된 DNA를 대상으로 계통학적 군집해석을 수행한 결과가 보다 더 다양한 계통군을 검출할 수 있음이 밝혀졌다. 한편, ISOIL kit를 이용하여 구축된 총clone중 약40%가${\alpha}-proteobacteria$ 계통군에 속하였으며, 약 30%가 ${\gamma}-Proteobacteria$ 계통군에 속하여 우점 계통군으로 확인된 반면, manual법에 의해 구축된 clone의 41%가 Acidobacteria 계통군에 속하였고 ${\alpha}-proteobacteria$(28%)가 우점 계통군으로 분포하는 계통학적 특징을 나타내어 DNA추출법에 따라 토양 세균군집 구조의 계통학적 특성 이 상이하게 나타나고 있음을 알 수 있었다. The principal objective of this study was to analyze 16S rDNA-ARDRA of the humus forest soil via an improved manual method and an ISOIL kit on the basis of the UPGMA clustering of the 16S rDNA combined profile, 44 ARDRA clusters of 76 clones via the ISOIL kit method and 45 ARDRA clusters of 136 clones via the improved manual method. On the basis of the 16S rDNA sequences, 44 clones from the ARDRA clusters by the ISOIL kit were classified into 3 phyla : ${\alpha}-,\;{\beta}-,\;{\gamma}-,\;{\delta}-Proteobacteria$, Acidobacteria and Actinobacteria. Using the improved manual method, the specimens were classified into 6 phyla : the ${\alpha}-,\;{\beta}-,\;{\gamma}-,\;{\delta}-Proteobacteria$, Acidobacteria, Bacteroides, Verrucomicrobia, Planctomycetes and Gemmatomonadetes. As a result, the modified manual method indicated greater phylogenetic diversity than was detected by the ISOIL kit. Approximately 40 percent of the total clones were identified as ${\alpha}-Proteobacteria$ and 30 percent of the total clones were ${\gamma}-Proteobacteria$ and assigned to dominant phylogenetic groups using the ISOIL kit. Using the modified manual method, 41 percent of the total clones were identified as Acidobacteria and 28 percent of total clones were identified as ${\alpha}-proteobacteria$ and assigned to dominant phylogenetic groups.
정상 성인에서 식이섬유 섭취량이 배변 습관 및 장관통과시간에 미치는 영향
김재준,최규완,백승운,고광철,이풍렬,성인경,심상군,임효근,이종철,전성국,손희성 대한소화기학회 2000 대한소화기학회지 Vol.35 No.1
Background/Aims: This study was designed to assess the normal bowel habit and effects of dietary fiber on bowel transit in healthy Koreans because such data are not sufficiently available. Methods: For 30 healthy volunteers (15 males, 15 females), we examined small bowel transit time using lactulose hydrogen breath test and colonic transit time using radiopaque markers. Total dietary fiber intake was assessed by dietary record for three days and bowel habit was assessed using questionnaires. Results: The most common bowel habit was once a day (24 hour cycle). Mean daily consumption of total dietary fiber was 15.2±5.2 g. Mean small bowel transit time was 89.4±24.4 min with no significant difference according to sex. Mean colonic transit time was 35.7±16.5 hours. Furthermore, there was a significant correlation between colonic transit time and the amount of total dietary fiber consumption. Conclusions: The significant correlation between the amount of total dietary fiber consumption and colonic transit time gests that decreased consumption of dietary fiber may be related to prolonging colonic transit time.