탄소원의 고갈에 따른 대장균 세포 내 단백질 분해 활성도의 조절

손종경,최경희,정진하 ( Jong Kyung Sonn,Kyung Hee Choi,Chin Ha Chung ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.4

E. coli contains two distinct groups of proteolytic enzymes: one is serine proteases that hydrolyze globin and casein and the other is metalloproteases degrading insulin and glucagon. The levels of both activities were increased several-fold when the cells were grown to stationary phase in Luria broth (LB) but not at all when they were cultured in M9-glucose medium. `The raise in globin-hydrolyzing activity of serine proteases was completely prevented by the addition of glucose in LB, while insulin-degrading activity of metalloproteases remained increasing under the same growth condition. Starvation for carbon source of the cells grown in M9-glucose medium casused a marked increase in the activity of serine proteases but not of metalloproteases. Furthermore, the level of serine proteases did not increase in a mutant cell that lacks adenylate cyclase when the cell was grown in LB to stationary phase while the activity of metalloproteases was persistently raised in the same cell. Thus, the level of serine proteases in E. coli appears to be regulated by a mechanism similar to catabolite repression and that of metalloproteases by a change of intracellular pool of free amino acids.

계배 wing bud 간충직세포의 연골화에 미치는 cAMP와 staurosporine의 영향

孫鍾京 경북대학교 과학교육연구소 1992 科學敎育硏究誌 Vol.16 No.-

To investigate the mechanisms of enhancing effect by cAMP and staurosporine on chondrogenesis, cultures of HH-stage 23/24 chick embryo limb bud mesenchymal cells were treated with cAMP, staurosporine or PMA on different culture days and quantitative analysis of chondrogenesis and morphological study were employed. Staurosporine showed chondrogenic effect in any time during the culture. In the cross section of staurosporine treated micromass cultures, larger and denserly Alcine blue stained nodules were observed. Several other inhibitors of PKC or protein tyrosine kinase did not show any significant effect on chondrogenesis. Chondrogenesis was promoted when cAMP was added to the cultures after 1 day of culture but there was no clear nodule in cAMP treated cultures. These data indicate that staurosporine promoted chondrogenesis by inducing cell condensation during the early stage of differentiation and cAMP affects chondrogenesis after cell condensation possibly by modulating gap junctional communication.

Regulation of Proteolytic Activities in Escherichia coli During Starvation of Carbon Source

손종경,최경희,정진하,Sonn, Jong-Kyung,Choi, Kyung-Hee,Chung, Chin-Ha 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.4

대장균세포는 globin과 casein을 분해하는 serine proteases(SP)와 insulin과 glucagon을 분해하는 metalloproteases(MP)의 두 부류의 단백질 분해효소들을 포함하고 있다. 대장균을 LB에서 stationary phase까지 배양할 때 나타나는 단백질 분해 효소들의 활성도는 log phase까지 배양할 때 보다 약 3-5배 증가되었다. 그러나, M9-glucose medium에서 배양하였을 때에는 전혀 증가를 보이지 않았다. 한편, LB에 포도당을 첨가하여 대장균을 배양하면 MP들의 활성도는 여전히 증가되었으나 SP의 것은 전혀 증가되지 않았다. 그리고, M9-glucose medium으로부터 탄소원을 고갈시킬 경우에, SP의 활성도는 현저히 증가되나, MP의 것은 증가되지 않았다. 뿐만 아니라, cAMP를 합성할 수 없는 cya돌연변이를 LB에서 stationary phase까지 배양할 때에는 SP의 활성도는 증가되지 않았으나 MP의 것은 여전히 증가되었다. 따라서, 대장균 세포 내의 SP의 발현은 catabolite repression과 유사한 기작으로 조절되며, MP의 발현은 세포 내 아미노산 농도의 변화 따라 조절되는 것으로 추측된다. E. coli contains two distinct groups of proteolytic enzymes: one is serine proteases that hydrolyze globin and casein and the other is metalloproteases degrading insulin and glucagon. The levels of both activities were increased several-fold when the cells were grown to stationary phase in Luria broth (LB) but not at all when they were cultured in M9-glucose medium. The raise in globin-hydrolyzing activity of serine proteases was completely prevented by the addition of glucose in LB, while insulin-degrading activity of metalloproteases remained increasing under the same growth condition. Starvation for carbon source of the cells grown in M9-glucose medium casused a marked increase in the activity of serine proteases but not of metalloproteases. Furthermore, the level of serine proteases did not increase in a mutant cell that lacks adenylate cyclase when the cell was grown in LB to stationary phase while the actvity of metalloproteases was persistently raised in the same cell. Thus, the level of serine proteases in E. coli appears to be regulated by a mechanism similar to catabolite repression and that of metalloproteases by a change of intracellular pool of free amino acids.

계배 wing bub 간충직세포의 연골화에 미치는 cAMP와 staurosporine의 영향

손종경 ( Jong Kyung Sonn ) 경북대학교 과학교육연구소 1992 科學敎育硏究誌 Vol.16 No.-

To investigate the mechanisms of enhancing effect by cAMP and staurosporine on chondrogenesis, cultures of HR-stage 23/24 chick embryo limb bud mesenchymal cells were treated with cAMP, staurosporine or PMA on different culture days and quantitative analysis of chondrogenesis and morphological study were employed. Staurosporine showed chondrogenic effect in any time during the culture. In the cross section of staurosporine treated micromass cul-tures, larger and denserly Alcine blue stained nodules were observed. Several other inhibitors of PKC or protein tyrosine kinase did not show any significant effect on chondrogenesis. Chondrogenesis was promoted when cAMP was added to the cultures after 1 day of culture but there was no clear nodule in cAMP treated cultures. These data indicate that staurosporine pro-moted chondrogenesis by inducing cell condensation during the early stage of differentiation and cAMP affects chondrogenesis after cell condensation possibly by modulating gap junctional com-munication.

A Role of Ca^(2+)/calmodulin-dependent Protein Kinase II in the Chondrogenic Differentiation of Chick Mesenchymal Cells In Vitro

Kim, Soo Dong,Sonn, Jong Kyung,Kang, Shin-Sung,Park, Tae Kyu 경북대학교 기초과학연구소 1994 慶北大學敎 基礎科學硏究所 硏究論文集 Vol.15 No.-

배양중인 계배간충직세포의 protein kinase C 활성도 분석

朴大圭,孫鍾京,鄭在昌 경북대학교 1992 논문집 Vol.24 No.-

To investigate the relationship between protein kinse C (PKC) and chondrogenesis, PKC activity was assayed in cultures of stage 23/24 chick limb bud mesenchymal cells under various conditions. PKC activities of cytosolic and particulate fractions were low in 1 day cultured cells. As chondrogenesis proceeds, cytosolic PKC activity increased more than 2-fold, while that of the particulate fraction increased only slightly. Three days' treatment of cultures with PMA(5×10 exp (-8)M) inhibited chondrogenesis judged by the accumlation of Alcian blue bound to the extracellular matrix and depressed PKC activity in cytosolic fraction. When cells were grown for 3 days in control medium after 3 days' treatment with PMA, chondrogenesis resumed and PKC activity recovered to normal values. PKC activity in cultures plated at low density (5×10 exp (6) cells/ml) or that in PMA treated cells. On the other hand, staurosporine promoted chondrgenesis without affecting PKC activity. Furthermore, reversal of PMA's inhibitory effect on chondrogenesis by staurosporine was not accompanied by recovery of PKC activity. These data indicate that increases in PKC activity is closely related to chondrogenesis and that PMA inhibits chondrogenesis by depressing PKC. However, staurosporine's enchancing effect of chondrogenesis is not related of PKC activity.

초,중등학교의 동,식물 영역에서 지도해야 할 개념에 대한 중등교사의 인식 조사

김영신,윤정주,손종경 韓國生物敎育學會 2009 생물교육 Vol.37 No.2

Scientific literacy is emphasized in mainstream science education all over the world, including Korea. BSCS classified the biological concepts to be taught in secondary schools and universities into essential, optional, and non-essential concepts for scientific-literacy training, and defined the range of biological concepts to be taught by school level. The purpose of this study was to confirm the animals and plants that should be taught in each school level, and to classify the concepts into essential, optional, and non-essential ones. A survey was conducted among secondary-school Biology teachers (146 respondents) and university Biology professors (29 respondents) from all over Korea to study the concepts of the animal and plant areas in elementary, middle, and high school. The results of the survey revealed the following. With regard to the teachers' recognition of the animal and plant domain concepts to be taught in elementary, middle, and high school, the number of essential concepts increased in every biological field as the school level went up. Moreover, there are much more animal and plant domain concepts that the teachers think should be taught in elementary and secondary school compared to those suggested in the curriculum. The biggest difference between the responses of the middle and high school teachers relates to the concepts that they think should be taught in middle school.

培養 鷄胚 筋細胞의 分化에 따른 數種 筋特異 蛋白質의 合成에 관하여

하두봉,유병재,손종경,강호성,이영섭 한국통합생물학회 1983 동물학회지 Vol.26 No.1

細胞의 分化에 관한 硏究의 一環으로 鷄胚의 筋細胞를 培養하면서 미오신, 악틴, 트로포미오신, 트로포닌 等 筋特異 蛋白質의 合成과 培養細胞에서 培養液內로 放出되는 蛋白質을 形態分化와 竝行하여 分析하였다. 筋特異 蛋白質중 미오신과 악틴은 細胞融合前에 활발히 合成되며 融合後에는 相對的으로 떨어지고, 트로포닌은 融合直後부터 활발히 合成되기 시작하며, 트로포미오신은 分化의 全期間에 걸쳐 合成율이 거의 一定하였다. 培養筋細胞는 細胞融合이 일어나기 前에 分子量 18,000 달톤과 20,000달톤 그리고 그 이상의 몇 가지 蛋白質을 培養液內에 放出시틴다는 것이 거의 確實하다. 이들 蛋白質은 培養筋細胞의 膜蛋白質일 것으로 推定되며 筋細胞의 同時 融合을 誘導하는 機能을 가진 것으로 생각된다. The synthesis of myosin, actin, tropomyosin and troponin in the cultured muscle cells of chick embryo during the differentiation were analyzed. The synthesis of myosin and actin were very active prior to the myoblast fusion while the troponin synthesis became active after the fusion. Tropomyosin was synthesized practically constantly throughout the culture period. Several proteins were detected in the muscle-conditioned medium strongly suggesting that the cells in culture released polypeptides which might act on the membrane of neighboring cells cells to initiate the fusion.

중등학교 과학과 교과서에서 환경단원의 실험을 위한 Kit 제작과 적용

양홍준,이윤호,권덕기,손종경,송방호,정화숙,박성호 韓國生物敎育學會 1999 생물교육 Vol.27 No.4

This paper was accomplished to manufacture of the kit for experimentation of the biological science laboratory classes in middle and high school since May 1996. The kit developed was named Bio-chamber. This kit was developed for inquiry learning and physiological experiments such as photosynthesis and transpiration of plants in biological and environmental chapters, and this kit was expected a lot of learning effect not only regular classes in text book but also extracurricular activities of students. We analyzed the question papers about the efficiency which are interests, understandings, application, and learning attitudes from the students who was educated by Bio-chamber. According to the result of investigation, most students preferred to experiment on Bio-chamber, 74.87% for affirmative. A case of where classify affirmative responses by localities, 79.38% for small and medium cities, 76.19% for rural communities, and 79.48% for large cities, large cities was low. On the other hand, negative responses showed in order 10.81% for large cities, 6.67% for rural communities, and 3.33% for small and medium cities.

X선에 의한 연골세포 분화 억제 작용경로

하종렬,임영빈,이윤애,손종경,이준일 대한방사선과학회(구 대한방사선기술학회) 2003 방사선기술과학 Vol.26 No.1