Inhibition of Xanthine Oxidase by Phenolic Phytochemicals from Broussonetia papyrifera

류형원,이지혜,강재은,진영민,박기훈 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5

The roots of Broussonetia papyrifera were extracted into four different polar solvents: chloroform, 50% ethanol in water, ethanol, and water. The ethanol extract showed the most potent inhibition (72.3% at 20 g/mL) against xanthine oxidase (XOD). Chromatography of EE yielded nine phenolic phytochemicals,which were confirmed as broussochalcone A (1), broussochalcone B (2), 3,4-dihydroxyisolonchocarpin (3), 4-hydroxyisolonchocarpin (4), 3-'(3-methylbut-2-enyl)-3',4',7-trihydroxyflavane (5), kazinol A (6), kazinol B (7), kazinol E (8), and broussoflavan A (9). All isolated compounds (19) possessed potent antioxidant activities against 2,2-diphenyl-l-picrylhydrazyl and 2,2'-azino-bis-ethylbenzthiazoline-6-sulfonic acid (ABTS) radicals with IC50 values ranging from 5.8 to 252.8M. Although most compounds exhibited potent inhibition with IC50 values ranging 0.6–164 M against XOD, compounds 1 and 3 were found to be the principal contributors to the XOD inhibition in ethanol extract. The analysis of KI and KIS values proved that the two most promising compounds (1 and 3), present at high concentrations in the root barks as analyzed by using high-performance liquid chromatography analysis, were reversible mixed type I inhibitors.

상속세및증여세법상 변칙증여에 대한 과세의 현황과 문제점

류형원 서울市立大學校 經商大學 稅務學科 2005 稅務學論集 Vol.18 No.1

상속세및증여세법상 변칙증여에 대한 과세의 현황과 문제점

Cu ( Ⅱ ) 이온이 Nalidixic Acid 의 DNA 와의 상호작용에 미치는 효과

류형원,오봉경,서정인,송창화,고동성 ( Hyeong Won Ryu,Bong Kyeong Oh,Jeong Ihn Suh,Chang Wha Song,Thong Sung Ko ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4

Nalidixic acid has been shown to be capable of direct binding with DNA in an intercalative fashion via the formation of relatively stable planar cationic 1:1 chelate complex with Cu(II) at physiological pH as studied by the absorption difference spectroscopic and fluorescence spectroscopic titration data, competitive binding experiments using ethidium bromide, and viscosity increase caused by the three-dimensional structural change of the DNA. The nearest neighbor exclusion type of direct intercalative binding of the Cu(II) complexes with double-stranded DNA between the base-pairs was not exhibited by the denatured DNA.

Cu(II) 이온이 Nalidixic Acid의 DNA와의 상호작용에 미치는 효과

류형원,오봉경,서정인,송창화,고동성,Ryu, Hyeong-Won,Oh, Bong-Kyeong,Suh, Jeong-Ihn,Song, Chang-Wha,Ko, Thong-Sung 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.4

생리적 pH에서의 흡수차이분광학적 적정 및 형광분광학적 적정 데이터로부터 $Cu^{2+}$는 nalidixic acid와 비교적 안정한 양하전의 평면성 킬레이트복합체(1 : 1)를 형성할 수 있으며 이 복합체는 유리(free) nalidixic acid의 경우와는 달리 DNA와의 직접적인 염기쌍 사이에의 nearest neighbor exclusion 방식의 intercalation 결합을 통한 상호작용을 할 수 있음을 변성 calf thymus DNA에서는 볼 수 없는 ethidium bromide의 그 본성 DNA와의 결합에 대한 이 복합체의 경쟁적 억제효과 특성으로부터 알 수 있었다. 또한 이 $Cu^{2+}$-nalidixate는 유리 nalidixic acid와는 달리 DNA에 intercalative 결합을 하므로써 DNA의 삼차원적 구조의 변형을 초래하여 점성도의 중가를 나타냈으며 변성 DNA에서는 볼 수 없는 이러한 점성도 증가의 특성으로부터 nearest neighbor exclusion intercalation의 가능성을 다시 확인할 수 있었다. Nalidixic acid has been shown to be capable of direct binding with DNA in an intercalative fashion via the formation of relatively stable planar cationic 1:1 chelate complex with Cu(II) at physiological pH as studied by the absorption difference spectroscopic and fluorescence spectroscopic titration data, competitive binding experiments using ethidium bromide, and viscosity increase caused by the three-dimensional structural change of the DNA. The nearest neighbor exclusion type of direct intercalative binding of the Cu(II) complexes with double-stranded DNA between the base-pairs was not exhibited by the denatured DNA.

염기성 폴리펩티드류에 의한 p-Nitrophenyl Acetate의 가수분해

류형원,맹학영,고동성 충남대학교 1996 忠南科學硏究誌 Vol.23 No.1

The hydrolysis of p-nitrophenyl acetate was catalytically promoted by the synthetic basic polypeptides. The first-order rate constants were linearly proportional to the tested concentration range (up to 4 mg/ml) of the basic polypeptides. The 1:1 (molar ratio of basic to hydrophobic residue) copolymer showed higher reactivity than the 4:1 copolymer. A plot of the logarithm of the second-order rate constants (Log K_cat/K_R) versus pK_a values of the basic residues showed linear-free-energy -relationship (LFER), and the slope was 0.4.

국내유통 감초의 대사체 프로파일 분석

류형원,오세량,이정훈,이윤지,정진태,박춘근,김용일,장재기 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.05

Background : To evaluate the quality of Glycyrrhizae radix (licorice), a popular herbal medicine in Korea, it is necessary to investigate the component profiles of the Glycyrrhiza species distributed in Korean market. Specifically, to control the quality of Korean Glycyrrhizae radix preparations including raw materials, glycyrrhizin (> 2.5%) and liquiritigenin (> 0.7%) has been used as a standard marker compound in Korea Food and Drug Administration (KFDA). To the best of our knowledge, there have been only a few studies regarding the fingerprinting techniques and metabolomics approach of the phytochemicals in Glycyrrhizae radix in Korean market. Therefore, it is necessary to develop a validated method for botanical raw material (BRM) standardization. Methods and Results : Twenty-one Glycyrrhizae radix (G. glabra, G. uralensis, and G. glabra × G. uralensis) were analyzed by UPLC-QTOF-MS and were estimated to statistical and multivariate analysis. Large scale multi-targeted metabolic profiling and fingerprinting techniques were utilized to access a broader insight into the chemical composition of Glycyrrhiza species. The UV/VIS spectra, and MS data (accurate mass and fragmentation pattern in negative mode) of the components in the BRM of G. glabra, G. uralensis, and G. glabra × G. uralensis (incorporation and hybridization) were analyzed with multivariate analyses. Interestingly, UPLC-QTof-MS derived PCA analysis and fingerprinting techniques was found to be effective techniques in the classification of species based on genetic and/or geographical origins. In addition, the revealed metabolite markers could be utilized as a guidance for the BRM standardization as well as a database for quality control of commercial products containing Glycyrrhizae radix. Conclusion : From the above results, the revealed metabolite markers could be utilized as a guidance for the BRM standardization as well as a database for quality control of commercial products containing Glycyrrhizae radix.

주성분 분석과 퍼지 논리를 활용한 대전 미세먼지 취약지도 제작에 관한 연구

류형원,장동호 건국대학교 기후연구소 2022 기후연구 Vol.17 No.1

A fine dust vulnerability map was generated to efficiently manage the concentration of fine dust pollution. By applying the fourteen indicators used for assessing the vulnerability to fine dust, including fine dust pollution source and concentration, fine dust vulnerability classes, and usage facilities, the vulnerable regions were derived through the analyses of the principal components and fuzzy logics. As a result of this study, it was displayed that in Daejeon, the greatest number of vulnerable regions were distributed in Yuseong-gu, followed by the order of Seo-gu, Daedeok-gu, Jung-gu, and Dong-gu. In Yuseong-gu, a traffic congestion section around the terminal, a large-scale apartment complex, and an energy industry combustion facility are located. Due to which, types of surface and mobile pollution, vulnerable groups, and point pollution appeared together. In Seo-gu, the type of surface and mobile pollution and vulnerable groups were seen in the areas adjacent to the city hall. This is because that large-scale apartment complexes, elementary schools, and administrative agencies are concentrated around the city hall, which leads to traffic congestion. It was identified that Jung-gu and Dong-gu are vulnerable groups because many large-scale apartment complexes, elementary schools, and rehabilitation hospitals are located in the regions. In Daedeok-gu, types of vulnerable groups were seen in the areas with aged population. Furthermore, types of pollution were shown in the regions where the Daedeok Industrial Complex, energy industry combustion facilities, and waste treatment facilities are located. The result of this study can be utilized as basic data to establish a customized policy for fine dust reduction according to the identified types of vulnerabilities.

미세먼지(PM₁₀) 오염농도와 토지피복간의 상관성 분석을 통한 GWR 모델의 적합성 평가

류형원 ( Ryu Hyeong-won ),장동호 ( Jang Dong-ho ) 한국사진지리학회 2019 한국사진지리학회지 Vol.29 No.1

본 연구는 미세먼지(PM₁₀) 오염농도와 토지피복간의 상관성 분석을 통해 GWR(Geographically Weighted Regression) 모델의 적합성 평가를 실시하였다. 특히, 미세먼지 특성상 공간 자기상관성이 있다고 판단되어 동일한 회귀 분석 모형에 일반최소자승법과 지리가중회귀분석 모델을 적용하여 결과를 비교하였다. 연구결과, 두 회귀분석 모델의 결과를 비교했을 때 지리가중회귀분석 모델의 결정계수 값이 높아 모형 설명력이 더 높은 것으로 나타났다. 또한 AICc(Akaike Information Criterion correction) 계수는 일반최소자승법보다 지리가중회귀분석에서 더 낮은 값을 보여 지리가중회귀분석을 통한 모델의 개선이 이루어졌다고 판단된다. Moran 지수를 이용하여 두 모델의 표준잔차에 대한 공간 자기상관성을 비교 분석한 결과, 일반최소자승법의 표준잔차에서는 정적 상관관계가 나타나 공간 자기상관성이 뚜렷하게 나타났지만, 지리가중회귀분석의 표준잔차에서는 무작위 패턴이 나타나 공간자기상관성을 모두 통제한 것으로 나타났다. 본 연구는 미세먼지 관련 연구에 있어 지리가중회귀분석의 적합성을 밝히고, 분석을 통해 얻어진 결과로 미세먼지 관련 연구에 기초데이터를 제공함으로써 국내 미세먼지 오염농도 저감에 기여하고자 하였다. This study was conducted to evaluate the suitability of the GWR model for the correlation analysis between fine particles(PM10) pollution concentration and land-cover. Especially, it was judged that there is spatial autocorrelation due to the characteristics of fine particles, and the results are compared by applying the ordinary least squares method and the geographically weighted regression analysis model to the same regression analysis model. As a result of the study, comparison of two regression analysis the geographically weighted regression model shows higher explanatory power. The Akaike information criterion correction(AICc) coefficient was also lower in the geographically weighted regression analysis than the ordinary least squares method. Therefore, it is considered that the model was improved through the geographically weighted regression analysis. The Moran index was used to compare the spatial autocorrelation of the standard residuals of the two models. In the standard residual of ordinary least squares method, static correlation appeared and spatial autocorrelation appeared. However, in the standard residuals of the geographically weighted regression analysis, random patterns appeared to control all spatial autocorrelation. This study showed that the suitability of geographically weighted regression analysis in the study of fine particles. As a result of the analysis, it was aimed to contribute to the reduction of the fine particles pollution concentration in Korea by providing basic data for the study of fine particles.

반하(Pinellia ternata)에서의 [6]-gingerol 함량과 멜라닌 저해 활성에 영향을 미치는 원산지 판별 마커로의 활용

류형원 ( Hyung Won Ryu ),안주현 ( Ju Hyeon An ),원효준 ( Hyo Jun Won ),서수경 ( Soo-kyung Seo ),김두영 ( Doo-young Kim ),구창섭 ( Chang-sub Ku ),오세량 ( Sei-ryang Oh ) 한국응용생명화학회(구 한국농화학회) 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.4

반하(Pinellia ternata Breitenbach)는 천남성과(Araceae)의 약용식물로 동아시아 일대가 원산지이며, 유럽과 북아메리카 일부지역에도 널리 분포하고 있는 식물이다. 반하의 괴경(tuber)은 약재로 사용되어 구토, 염증, 외상을 치료하는 목적으로 사용되고 있다. 보고된 약리학적 작용으로는 항경련, 항종양, 살충, 세포독성 등이 있는 것으로 알려져 있다. 반하는 약리학적으로 유용한 약용식물로 잘 알려져 있음에도 불구하고, 원산지를 판별할 수 있는 신뢰성이 있고 표준화된 방법이 없는 실정이다. 이를 위한 한국산과 중국산 반하를 판별할 수 있는 유의적인 화합물을 탐색하기 위해 UPLC-PDA와 QTof-MS에 기반을 둔 대사체 표지법을 이용하였다. 한국산으로부터 원산지 판별 화합물로 예상되는 화합물을 반복적인 역상 칼럼크로마토그래피에 기반을 둔 활성유도 분획법을 통해 분리하였다. 그리고, NMR과 MS를 포함한 물리화학적, 분광학적 정보의 결과를 토대로 [6]- gingerol이라는 화합물의 구조를 동정하였다. [6]-gingerol은 원산지를 판별할 수 있는 능력과 melanin 생합성 저해 활성을 지닌 기능성 화장품 소재로서 가치가 있다고 판단되어짐에 따라 유효성분 원산지 판별 화합물로 Fingerprint법에 의해 선정되었다. 더욱이, 한국산과 중국산 반하 유래 [6]-gingerol의 함량 비교를 위해, 유효성이 검증된 분석법을 이용하여 이에 대한 검량곡선을 작성하여 그 함량을 비교하였다. 이것은 한국산 반하판별을 위한 화합물 선정과 성공적인 유효성 검증을 다룬 최초의 보고이다. Pinellia ternata Breitenbach, the natural medicinal plant of the Araceae family, is a perennial plant originated from the East Asia, but also widely distributed in Europe and North America. Its tuber is used as traditional medicine for treatment of various diseases such as vomiting, inflammation, and traumatic injury. Pharmacological studies revealed that P. ternata possesses anticonvulsant, anti-tumor, insecticidal, and cytotoxic activities. Despite being well-known as the useful medicinal plant, there is no reliable, standardized method for origin discrimination. Ultra performance liquid chromatography-photodiode array detector and quadrupole time of flight-mass spectrometry based metaboliteprofiling was applied to explore significant metabolite for origin discrimination between Korean and Chinese P. ternata. One compound was isolated from Korean P. ternata using repeated ODS column chromatography by bioactivity guided fractionation, and determined as [6]-gingerol according to the results of spectroscopic data including nuclear magnetic resonance and MS. This compound was selected as cosmeceutical biomarker by fingerprints, and it was associated to melanin inhibitory effect determining its origin authenticity. Furthermore, the calibration curve of biomarker was prepared using validated method for the comparison of content between Korean and Chinese P. ternata. This is the report to address the selection and successful validation of the discriminant metabolite for confirmation of Korean P. ternata.

다중시기 위성영상을 이용한 제주도 중산간지역의 토지피복 변화 탐지

류형원 ( Ryu Hyeong Won ),장동호 ( Jang Dong-ho ) 한국사진지리학회 2018 한국사진지리학회지 Vol.28 No.3

본 연구는 다중시기의 위성영상(1981∼2017년)을 이용하여 제주도 중산간지역의 토지피복 변화를 탐지하고 변화요인을 분석하고자 하였다. 연구결과, 1981년부터 2017년까지 수역, 농경지, 과수원, 시가화지역, 나대지, 습지는 지속적으로 증가하였으며, 초지는 꾸준히 감소하였다. 산림지의 경우 1991년에는 증가하다가 2004년에는 감소하고, 2017년에는 다시 증가하여 결과적으로 1981년에 비해 면적이 크게 증가한 것이 특징적이었다. 이는 점진적으로 규모가 커진 조림사업, 축산업 쇠퇴에 따른 농경지 개간, 관광시설 및 공공건물 건축부지 전용, 도로 건설 및 인프라 구축, 골프장 건설 등에 의한 것으로 판단된다. 이러한 결과들을 종합하여 보면, 본 연구는 다중시기의 위성영상을 이용해 토지피복도를 작성하여 중산간지역의 토지피복 변화를 탐지하였고, 그 결과를 토대로 변화 요인을 분석할 수 있었다. 이와 같은 결과들은 제주 중산간지역의 지속가능한 개발 계획 수립 및 효율적인 관리에 기초자료로 활용될 수 있을 것이라고 기대된다. This study intended to detect the changes of land-cover in the mid-mountain area of Jeju Island and to analyze the factors of change using multi-temporal satellite images(1981∼2017). The result of this study displayed that waters, farmlands, orchards, urban areas, barelands, and wetlands continually increased from 1981 to 2017, and grasslands consistently decreased. Forests area increased in 1991 and decreased in 2004, then increased again in 2017, and as a result displayed a large increase in the surface area compared to 1981. It is determined that this is based on the cultivation of farmlands, use of tourism facilities and public buildings as lands for architectural sites, construction of roads and infrastructures, and construction of golf courses due to the gradually increased scale of the forestation industry and the declining of the livestock industry. Upon combination of the foregoing results, this study detected the change in the land coverage in the mid-mountain area of Jeju Island using multi-temporal satellite images, and was able to analyze the factors of change based on such results. These results are expected to be used as basic data for the establishment of a plan for the sustainable development and efficient management of the mid-mountain area of Jeju Island.