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Histological Comparison Study with Primo Node and Immature Liver Tissue on Liver Surface in Rat
류연희,이성원,황성연,김민수,반효정,최선미,김재효,손인철,안성훈,Ryu, Yeon-Hee,Lee, Sung-Won,Hwang, Sung-Yeoun,Kim, Min-Su,Ban, Hyo-Jeong,Choi, Sun-Mi,Kim, Jae-Hyo,Sohn, In-Chul,Ahn, Seong-Hun Society for Meridian and Acupoint 2011 Korean Journal of Acupuncture Vol.27 No.4
목적 : 프리모 모드와 미성숙 간조직에 대한 조직학적 특성을 비교하고자 하였다. 방법 : 흰쥐에서 간절제한 후, 간장기 표면에서 관찰되는 프리모 모드와 그와 유사한 미성숙 간조직을 H&E, Oil red O, Masson trichrome and van Gieson 염색방법을 통해서 비교 연구하였다. 결과 : 상기 실험 연구를 진행한 결과, 다음과 같은 결론을 얻었다. 1. 조직의 일반적인 특징을 관찰할 수 있는 H&E 염색결과, 프리모 노드에서는 많은 수의 조그마한 동(sinus)이 관찰되었고 적혈구나 영양을 공급하는 혈관의 분포는 관찰되지 않았다. 이와 반대로 미성숙 간조직에서는 동이 관찰되지 않았으며 혈관의 분포와 적혈구가 관찰되었다. 2. 양 조직의 지방성분의 유무를 관찰하기 위한 Oil red O 염색결과, 프리모 노드에서는 지방성분이 관찰되지 않았으나 미성숙 간조직에서는 관찰되었다. 3. 양 조직의 콜라겐성분의 유무를 관찰하기 위한 Masson trichrome and van Gieson 염색결과, 프리모 노드에서는 약간의 콜라겐 성분이 관찰되었으나 elastic 성분은 관찰되지 않았으며, 미성숙 간조직에서는 콜라겐 성분과 elastic 성분이 관찰되지 않았다. 결론 : 상기 결과는 프리모 노드와 간조직과는 조직학적 성질이 다른 것으로 사료되며, 특히 콜라겐 성분의 적은 결과는 프리모 노드가 불규칙한 형태를 이루고 있는 이유에 대한 실마리를 제공하였다. 이러한 결과는 프리모 노드의 특성에 대한 기본 자료로 활용될 수 있을 것이다.
흰쥐의 간 장기 표면에서 관찰되는 프리모 노드와 미성숙 간조직과의 조직학적 비교연구
류연희 ( Yeon Hee Ryu ),이성원 ( Sung Won Lee ),황성연 ( Sung Yeoun Hwang ),김민수 ( Min Su Kim ),반효정 ( Hyo Jeong Ban ),최선미 ( Sun Mi Choi ),김재효 ( Jae Hyo Kim ),손인철 ( In Chul Sohn ),안성훈 ( Seong Hun Ahn ) 경락경혈학회 2011 Korean Journal of Acupuncture Vol.28 No.1
목적: 프리모 모드와 미성숙 간조직에 대한 조직학적 특성을 비교하고자 하였다. 방법: 흰쥐에서 간절제한 후, 간장기 표면에서 관찰되는 프리모 모드와 그와 유사한 미성숙 간조직을 H&E, Oil red O, Masson trichrome and van Gieson 염색방법을 통해서 비교 연구하였다. 결과: 상기 실험 연구를 진행한 결과, 다음과 같은 결론을 얻었다. 1. 조직의 일반적인 특징을 관찰할 수 있는 H&E 염색결과, 프리모 노드에서는 많은 수의 조그마한 동(sinus)이 관찰되었고 적혈구나 영양을 공급하는 혈관의 분포는 관찰되지 않았다. 이와 반대로 미성숙 간조직에서는 동이 관찰되지 않았으며 혈관의 분포와 적혈구가 관찰되었다. 2. 양 조직의 지방성분의 유무를 관찰하기 위한 Oil red O 염색결과, 프리모 노드에서는 지방성분이 관찰되지 않았으나 미성숙 간조직에서는 관찰되었다. 3. 양 조직의 콜라겐성분의 유무를 관찰하기 위한 Masson trichrome and van Gieson 염색결과, 프리모 노드에서는 약간의 콜라겐 성분이 관찰되었으나 elastic 성분은 관찰되지 않았으며, 미성숙 간조직에서는 콜라겐 성분과 elastic 성분이 관찰되지 않았다. 결론: 상기 결과는 프리모 노드와 간조직과는 조직학적 성질이 다른 것으로 사료되며, 특히 콜라겐 성분의 적은 결과는 프리모 노드가 불규칙한 형태를 이루고 있는 이유에 대한 실마리를 제공하였다. 이러한 결과는 프리모 노드의 특성에 대한 기본 자료로 활용될 수 있을 것이다.
Magnetocardiography(MCG)를 이용한 심병증의 진단에 관한 임상연구
송낙근,류연희,문진석,안규석,최선미,Song, Nak-Kun,Ryu, Yeon-Hee,Moon, Jin-Suk,Ahn, Kyoo-Seok,Choi, Sun-Mi 한국한의학연구원 2004 한국한의학연구원논문집 Vol.10 No.2
The aim of this study is to confirm clinical usefulness of MCG data by analyzing korea medical results of heart disease patients. We used the Heart Disease Questionnaire which asks for Qi deficiency-pattern, Blood deficiency-pattern, Yin deficiency-pattern, Yang deficiency-pattern, Qi stasis-pattern, Blood stasis-pattern, Heart heat-pattern, Phlegm-pattern. Magnetocardiography(MCG) is the measurement of magnetic fields emitted by the human heart from small currents by electrically active cells of the heart muscle. Comparing the MCG results and korea medical diagnosis, we showed clinical usefulness of MCG results and korea medical diagnosis.
신문혈(神門穴) 침자극(鍼刺戟)이 모성분리(母性分離) 스트레스로 야기된 섭식(攝食) 장애(障碍)와 시상하부 neuropeptide Y 발현에 미치는 영향
박히준,류연희,홍미숙,김승태,임사비나,Park, Hi-joon,Ryu, Yeon-hee,Hong, Mee-suk,Kim, Seung-tae,Lim, Sabina 대한침구의학회 2003 대한침구의학회지 Vol.20 No.4
Objective: The purpose of this study is to find out the effect of acupuncture at HT7 (Shinmun) on the feeding behavior and hypothalamic neuropeptide Y(NPY) expression in the maternally separated rat pups. Methods: To show the effect of acupuncture, we performed maternal separation(MS) for 7 days beginning on postnatal day 14, and observed body weight, food intake, and NPY immunoreactivity in the paraventricular nucleus (PVN) of hypothalamus after acupuncturing at HT7, the end of the transverse crease of the ulnar wrist of the forepaw. Results: MS induced a significant decreases in body weight and food intake, while acupuncture treatment at acupoint HT7 showed much more improvement in those evaluations. NPY-immunoreactivity in area PVN were decreased in the MS group, but significantly increased in the HT7 group. Conclusions: These findings suggest that acupuncture has an effect on the feeding disorders caused by MS, possibly by modulating NPY expression in the PVN.
천화분 약침액의 A549 폐암 세포주에서 apoptosis 유발효과
최태연 ( Tae Yeon Choi ),이성원 ( Sung Won Lee ),류연희 ( Yeon Hee Ryu ),반효정 ( Hyo Jeong Ban ),서근영 ( Geun Young Seo ),김재효 ( Jae Hyo Kim ),안성훈 ( Seong Hun Ahn ),손인철 ( In Chul Sohn ) 경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.4
Objectives: In order to confirm the anti-cancer effect of Trichosanthes kirilowii pharmacopuncture fluid, this study was proceeded. Methods: A549 lung cancer cells were cultured to be treated by Trichosanthes kirilowii pharmacopuncture fluid as dose dependent manner for 72 hours. And then the cell viability, nucleus fragmentaion, p21 and p53 protein expression, Bcl-2 and Bax protein expression, procaspase-3 PARP protein expression. Results: 1. Trichosanthes kirilowii pharmacopuncture fluid decrease A549 cell viability as dose dependent manner. 2. Trichosanthes kirilowii pharmacopuncture fluid induced the nucleus fragmentation in A549 lung cancer cells as dose dependent manner. 3. Trichosanthes kirilowii pharmacopuncture fluid increase the p21 and p53 protein expression. 4. Trichosanthes kirilowii pharmacopuncture fluid decrease the Bcl-2 protein expression but cannot affect the Bax protein expression. 5. Trichosanthes kirilowii pharmacopuncture fluid increase the activation of caspase-3 and PARP protein. Conclusions: As the above results, it was conclused the Trichosanthes kirilowii pharmacopuncture fluid had the anti-cancer effects to induce apoptosis.
한방임상사용 의료기기의 실태 조사를 통한 개선방향 제시
문진석,김종열,류연희,송낙근,김혁주,이정림,이재원,최선미,Moon, Jin-Seok,Kim, Jong-Yeol,Ryu, Yeon-Hee,Song, Nak-Kun,Kim, Hyeog-Ju,Lee, Jeong-Rim,Lee, Jae-Won,Choi, Sun-Mi 한국한의학연구원 2005 한국한의학연구원논문집 Vol.11 No.1
We conduct a survey of actual condition of medical devices in korea traditional medicine on 157 oriental medicine doctors and 183 manufacturers. The 61% percent of doctors indicate the trouble in use of medical devices which is applied to korea traditional medicine. The manufacturers replied that devices have a problem with diagnosis(53%) and treatment(36%). To overcome these problems, it is necessary to develop devices based on the study of basic oriental medicine and the reliable clinical research. This study contributes the improvement of quality and the revision of rules on medical devices.
침자극(鍼刺戟)이 생식능력(生殖能力) 저하(低下) 닭에 미치는 영향(影響)
강대희 ( Dae Hee Kang ),손양선 ( Yang Sun Son ),류연희 ( Yeon Hee Ryu ),전익수 ( Ik Soo Jeon ),박히준 ( Hi Joon Park ),임사비나 ( Sa Bi Na Lim ) 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.3
Objectives: To investigate the increasing effect of veterinary acupuncture (VA) on the eggs-laying rate, we carried out the experiment in old hens. Methods: Twenty four old hens (ISA Brown; 1,200-1,400 g) over 75 weeks old whose eggs-laying rates were under the 50% for recent 15 days were used. In experimental group, VA treatments were performed seven times during 10 days. Unang point at the end of bilateral wing and Migun point at the tail bone were stimulated about 30 seconds by the stainless steel needle (0.3 mm o.d.). In control group, similar manual stress were performed at same frequency. The numbers of laid eggs were observed for 26 days after the beginning of treatment. Bloods were sampled two times from wing veins during and after the treatment period and serum concentrations of luteinizing hormone (LH) and Estradiol were detected. Results: Eggs-laying rates of experimental group (34.29±2.79%) were significantly increased than that of control group (27.56±2.00%, P < 0.05) with the significant increasing of LH serum concentration in experimental group (from 0.77±0.13 IU/I to 1.42±0.22 IU/I, P<0.05). Conclusions: Increasing effect of VA treatment on the eggs-laying rate in old hens were proved in this study, and it may be the first prompt on the experimental investigation and development of VA.
백회(百會)(GV20),신會(회)(GV22) 자침이 뇌파에 미치는 영향
이상훈 ( Sang Hun Lee ),류연희 ( Yeon Hee Ryu ),권오상 ( O Sang Kwon ),손인철 ( In Chul Sohn ) 경락경혈학회 2012 Korean Journal of Acupuncture Vol.29 No.3
Objectives : The aim of this study was to examine the effects of Acupuncture at the GV20 and GV22 on normal human beings using power spectrum analysis. Methods : Electroencephalogram(EEG) power spectrum exhibits site-specific and state-related differences in various frequency bands. 8 channels Background Electroencephalogram (EEG) was carried out in 30 subjects(24 females and 4 males). Results : In δ(theta) band, the power values decreased significantly at the 8-channel average value( p=0.03) and especially at T3( p=0.02), T4( p=0.001) and P3( p=0.03). In α(alpha) band, the power values have no significant changes. In β(beta)band, the power values increased significantly at the 8-channel average value ( p=0.02) and especially at T4( p=0.003), P3 ( p= 0.03) and P4(0.02). In β/δ(beta/theta) ratio, the value increased significantly at the 8-channel average value(p=0.002) and especially at Fp2( p=0.05), F4( p=0.007), T3(0.012), T4(0.005), P3 (0.007) and P4(0.03) Conclusions : Through this data, we conclude that acupuncture at the GV20 and GV22 on normal human beings could have possibility to awake the cerebral cortex by the functional mechanism.
염전보사(捻轉補瀉)를 시행(施行)한 육음경승격혈위침자(六陰經勝格穴位鍼刺)가 고지방식이(高脂肪食餌) 비만백서(肥滿白鼠)에 미치는 영향(影響)
나창수 ( Chang Su Na ),김상훈 ( Sang Hoon Kim ),류연희 ( Yeon Hee Ryu ),최선미 ( Sun Mi Choi ),최찬헌 ( Chan Hun Choi ),윤대환 ( Dae Hwan Youn ) 대한경락경혈학회 2008 Korean Journal of Acupuncture Vol.25 No.4
Objectives: This research was performed to investigate the effect of five element constitutional acupuncture(Sa-am acupuncture) therapy at acupoints on the Yin meridians on obese rats(Sprague Dawley strain, male) induced by high fat diet. Methods: The control and acupuncture groups were provided with high fat diet, and the acupuncture groups were treated with five element constitutional acupuncture(Sa-am acupuncture) every other day for five weeks. We observed the body weight gain, food intake, triglyceride, total cholesterol, HDL-cholesterol, AST, and ALT. Result: The acupuncture group treated with five element acupuncture on the meridians of spleen, heart, pericardium showed significant decrease of body weight, the groups treated on the meridians of Lung, heart, pericardium showed signigicant decrease of food intake. Concentration of HDL-cholesterol was significantly increased in the groups treated on the meridians of spleen, heart, pericardium, Liver. Conclusion: Reviewing these experimental results, it appears that the five element constitutional acupuncture(Sa-am acupuncture) therapy at acupoints on the meridians of spleen, heart and pericardium have an efficacy on obesity induced by high fat diet.