전남 함평산 자색양파 추출물의 항균효과와 피부장벽 보호효과

윤대환,신헌태,Youn, Dae-Hwan,Shin, Heon-Tae 대한예방한의학회 2012 대한예방한의학회지 Vol.16 No.1

Objective : In this study, we assessed the anti-bacterial effects and epidermal permeability barrier function of red onion juice comparing to yellow onion juice and $Houttuynia$ $cordata$ extract $in$ $vitro$. Methods : 3types of red and yellow onion juice were prepared as antibacterial agent candidates with Houttuynia cordata hot water extract using 4 different bacterial strains ($Escherichia$ $coil$, $Salmonella$ $enterica$ $subsp.$ $enterica$, $Staphylococcus$ $epidermidis$, $Staphylococcus$ $aureus$ $subsp$) by colony counting method. The expression of filaggrin, a marker of keratinocyte differentiation, and serine palmitoyl transferase (SPT), a marker of the formation of the stratum corneum lipid barrier, in human HaCat keratinocytes were analyzed using HaCaT cell line. The expression of COX-2 and AP-1 which is a factor of COX-2 transcription were also analyzed by western blotting method. Results : There was detectable anti-bacterial effects on $Staphylococcus$ $epidermidis$, $Staphylococcus$ $aureus$ $subsp$ among 1%, 5%, 10% extracts of yellow and red onion.(81%-100%) The bacteriocidal effects were not shown on $Escherichia$ $coil$, $Salmonella$ $enterica$ $subsp.$ $enterica$ among $Houttuynia$ $cordata$, yellow onion and red onion extracts. The in vitro results showed the concentration-dependent effects on the expression of both filaggrin and SPT in HaCat cells among 0.01%, 0.05%, 0.1%, 0.5% extracts in Houttuynia cordata and red onion, reflecting the notion that $Houttuynia$ $cordata$ and red onion can induce epidermal keratinocyte differentiation and improve the recovery of skin barrier functions. The concentration-dependent effects also have been shown on the expression of both COX-2 and AP-1 among 0.01%, 0.05%, 0.1%, 0.5% extracts in $Houttuynia$ $cordata$ and red onion, while slight effect in yellow onion. Conclusion : Red onion juice could be a potential candidate enhanser for the skin care and cosmetology. 1. 추출방법, 추출농도에 따라 항균력의 차이가 있었으나, 일반양파, 자색양파의 1%, 5%, 10% 생즙추출물 모두, 피부상재균인 Staphylococcus epidermidis(KCTC1917), Staphylococcus aureus subsp(ATCC6538)에 양호한 항균력을 보였다(81%~100%). 어성초 열수추출물은 10% 고농도에서만 피부상재균인 Staphylococcus epidermidis(KCTC1917), Staphylococcus aureus subsp(ATCC6538)에 항균력을 보였다(100%). 2. 자색양파, 어성초, 일반양파의 1%, 5%, 10% 추출물 모두, 식중독 균인 Escherichia coil, Salmonella enterica subsp. enterica에서는 항균력을 보이지 않았다. 3. 자색양파 추출물과 어성초 추출물을 0.01%, 0.05%, 0.1%, 0.5%로 처리하였을 때, 피부장벽 조절인자인 filaggrin과 SPT의 발현을 농도 의존적으로 증가시켰다. 반면, 일반양파 추출물도 농도 의존적으로 filaggrin과 SPT발현양을 증가시켰지만, 자색양파 추출물에 비하여 발현양이 많지 않았다. 4. 피부 염증 반응 인자에 미치는 영향을 알아보기 위해서 COX-2의 발현과 AP-1의 발현을 분석한 결과, 자색양파와 어성초의 0.01%, 0.05%, 0.1%, 0.5% 추출물에서 농도 의존적으로 COX-2의 발현과 AP-1의 발현이 감소하였다. 반면 일반양파 추출물도 농도 의존적으로 COX-2의 발현과 AP-1의 발현이 감소하였으나, 자색 양파 추출물에 비하여 감소양이 적었다. 5. 자색양파 추출물과 어성초 추출물은 피부 장벽기능 조절인자인 filaggrin과 SPT의 발현양을 증가시켜 줌으로써, 피부의 수분 유지 기능을 강화하였고, 농도 의존적으로 피부 염증 반응 인자도 감소시켜 항염증효과가 있어, 피부미용기능재료로서의 가능성 보여주었다.

고지방식이(高脂肪食餌)로 유발(誘發)된 비만백서(肥滿白鼠)모델에서 창출(蒼朮), 의이인(薏苡仁), 마황(麻黃), 창출합의이인(蒼朮合薏苡仁) 마황합록차(麻黃合綠茶) 약침(藥鍼)이 혈청지질(血淸脂質) 및 간기능(肝機能)에 미치는 영향(影響)

윤대환,강자돈,주준성,채우석,나창수,Youn, Dae-Hwan,Kang, Ja-Don,Joo, Joon-Sung,Chae, Woo-Seok,Na, Chang-Su 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.1

Objective : This research was performed to investigate the effect of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Method : Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and Atractylodes japonica-herbal acupuncture group(AJ), high fat diet and Coix lachrymajobi-herbal acupuncture group(CL), high fat diet and Ephedra sinica-herbaI acupuncture group(ES), high fat diet and Atractylodes japonica+Coix lachrymajobi-herbal acupuncture group(AJ+CL), Ephedra sinica+Green tea-herbal acupuncture group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 132.5mg/kg body weight per 2day. Results : Body weight and food efficiency were decreased in AJ, ES, AJ+CL, ES+GT. The level of serum total cholesterol, triglyceride and free fatty acid were increased in AJ, ES, ES+GT. That of serum HDL-cholesterol was increased in AJ. The change of food intake, the level of serum phospholipid and ALP were not significant. The HDL to Total cholesterol ratio was increased in AJ and ES. Conclusion : Atractylodes japonica-herbal acupuncture in ST40 SP9 is effective on Body weight, food efficiency ratio, the level of serum lipid, protection of liver function and prevention cardiovascular risk by obesity induced by high fat diet. Herbal acupuncture mixed Ephedra sinica with Green tea can control the body weight, food efficiency ratio and the level of serum lipid.

대돈(大敦)${\cdot}$용천(湧泉) 염전(捻轉) 및 영수(迎隨) 보사수기법(補瀉手技法)이 2K1C에 의하여 유발(誘發)된 고혈압(高血壓) 백서(白鼠)에 미치는 영향(影響)

윤대환,한정희,김선희,나창수,Youn, Dae-Hwan,Han, Jeong-Hee,Kim, Suhn-Hee,Na, Chang-Su 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives : The purpose of this study was to examine effects of acupuncture by needle manipulation at $LR1{\cdot}Kl1$ on the blood pressure, plasma levels of ANP(atrial natriuretic peptide), renin and cardiomegalic index in hypertensive rat Induced by two kidney one clip(2K1C). Material and methods : The groups divided into 7 groups; Control, no treatment. Acu-1,acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 6 times. Acu-2, acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. Acu-3, acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was inserted in the opposite direction(body direction) as the channel runs, Acu-4, acupuncture at $LR1{\cdot}Kl1$ bilaterally, the needle was inserted in the opposite direction(body direction) as the channel runs and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. The acupuncture executed 5times for 2 weeks. Results : Systolic blood pressure was decreased significantly in Acu-2, Acu-3 and Acu-4. Plasma renin was decreased significantly in Acu-2 and Acu-4. Plasma ANP was increased significantly in Acu-2, however was decreased significantly in Acu-4. Cardiac hypertrophy index was decreased significantly in Acu-1, Acu-2, Acu-3 and Acu-4. Conclusions : These results suggest that acupuncture by needle manipulation at $LR1{\cdot}Kl1$ is effective in hypertension by renal blood problem.

족삼리(足三里) 침자(鍼刺)가 인체(人體)의 Skin temperature, Galvanic skin response 및 Heart rate에 미치는 영향(影響)

윤대환,나창수,Youn Dae-Hwan,Na Chang-Su 경락경혈학회 2003 대한침구의학회지 Vol.20 No.3

Objectives : The purpose of this study was to investigate the Effect of Acupuncture Chok-samni(ST36) on Skin temperature(ST), Galvanic skin response(GSR) and Heart rate(HR) in humans. ST36 is used in uneasiness, high fever, stroke, paralysis on oriental medicine. Methods : Subjects were 18 healthy oriental medical students volunteers(15male, 3female, mean age-28). Under sequence of ST36 acupuncture, the first group was observed ST, the second group was observed GSR and the third group was observed HR. Skin temperature was measured on Haegye acupoint(ST41) before and after acupuncture stimulation. Each test took 15minutes. This test was performed twice in order to increase the statistical authenticity. Results : Our result indicates that acupuncture stimulation of ST36 statistically decreased skin temperature on ST41. Also statistically decreased in HR. The change of GSR after ST36 acupuncture was decreased, but there is no statistical. Conclusions : These results suggested that acupuncture stimulation of ST 36 has effect on ST and HR in human body, and may be used by treatment as control the nerve system.

직자(直刺), 영수(迎隨) 및 염전수기법(捻轉手技法)에 따라 시행한 경거(經渠)${\cdot}$복류(復溜) 침자(鍼刺)가 중대뇌동맥(中大腦動脈) 폐새(閉塞)에 의하여 유발(誘發)된 국소(局所) 뇌허혈(腦虛血) 백서(白鼠) hippocampus의 항세포자멸사(抗細胞自滅死) 및 복경보호(福

윤대환,변정윤,최찬헌,백진웅,정지연,정연진,나창수,Youn, Dae-Hwan,Byun, Jeng-Yun,Choi, Chan-Hun,Baek, Jin-Ung,Jeong, Ji-Yeon,Jung, Yeon-Jin,Na, Chang-Su 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives : Aims of this study is to investigate the effects of $LU8{\cdot}KI7$ in rat induced by experimental focal ischemia. Materials and methods : The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The groups divided into 6groups, control; no therapy group after ischemia-induced, AT1; acupuncture therapy group at $LU8{\cdot}KI7$ after ischemia-induced, AT2; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 9times, AT3; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 9times, AT4; acupuncture therapy at$LU8{\cdot}KI7$ bilaterally and the needle was inserted to the direction following the flowing route of the meridian(digital direction), AT5; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally, the needle was inserted to the direction following the flowing route of the meridian(digital direction) and the needle was twirled and rotated forward with the thumb of the right hand 9times. Acupuncture therapy was carried out 7times during 2weeks after focal ischemia-induced. The anti-apoptotic and neuroprotective effects of acupuncture are observed by Bax, Bcl-2, mGluR5, cytochrome c, Cresyl violet and ChAT-stain. Results : The intensity of Bax was decreased in AC1, AC4, AC5 group, was increased in AC2, AC3 group. The intensity of Bcl-2 was increased in AC2, AC3, AC4, AC5 group. The intensity of mGluR5 was decreased in AC1 group, was increased in AC4, AC5 group. The intensity of Cytochrome c was increased in ACI, AC2 group, was decreased in AC4, AC5 group. The density of neurons stained by Cresyl violet was increased in all group without control group. The density of ChAT was increased in AC2, ACS group. Conclusions : Our study suggests that AC5 group show anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.

마황(麻黃), 마황합록차(麻黃合綠茶) 약침(藥鍼)이 고지혈증(高脂血症) 유발(誘發) 백서(白鼠)에 미치는 영향(影響)

윤대환 ( Dae Hwan Youn ),강자돈 ( Ja Don Kang ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.1

Objectives: This research was performed to investigate the effect of herbal acupuncture(Ephedra sinica and Green tea) at Umnungchon(SP9) and Pungnyung(ST40) on weight gain, food intakes, food efficiency ratios, HDL-cholesterol, triglyceride, free fatty acid on rats fed high fat diet for 5weeks. Methods: Experimental groups were divided into normal group(Sham), high fat diet group(Con), high fat diet and Ephedra sinica-herbal acupuncture in SP9·ST40 treated group(ES), high fat diet and Green tea-herbal acupuncture in SP9·ST40 treated group (GT), high fat diet and Ephedra sinica+Green tea-herbal acupuncture in SP9·ST40 treated group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 19.125mg/ml per 2 days during 5 weeks. Results: Body weight, feed intake and feed efficiency ratio were decreased in Sham, ES, ES+GT, compared with control group. The level of serum HDL-cholesterol was increased in sham, tender to increase in ES, ES+GT. The change on that of serum triglyceride was decreased in sham, ES, ES+GT and free fatty acid was decrease in sham, ES+GT. Conclusions: Ephedra sinica with Green tea-herbal acupuncture in SP9·ST40 can control the Body weight, feed intake and feed efficiency ratio, HDL-cholesterol, triglyceride, free fatty acid.

족삼리(足三里) 침자(鍼刺)가 인체(人體)의 Skin temperature, Galvanic skin response 및 Heart rate에 미치는 영향(影響)

윤대환 ( Dae Hwan Youn ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.3

Objectives: The purpose of this study was to investigate the Effect of Acupuncture Chok-samni(ST36) on Skin temperature(ST), Galvanic skin response(GSR) and Heart rate(HR) in humans. ST36 is used in uneasiness, high fever, stroke, paralysis on oriental medicine. Methods: Subjects were 18 healthy oriental medical students volunteers(15male, 3female, mean age-28). Under sequence of ST36 acupuncture, the first group was observed ST, the second group was observed GSR and the third group was observed HR. Skin temperature was measured on Haegye acupoint(ST41) before and after acupuncture stimulation. Each test took 15minutes. This test was performed twice in order to increase the statistical authenticity. Results: Our result indicates that acupuncture stimulation of ST36 statistically decreased skin temperature on ST41. Also statistically decreased in HR. The change of GSR after ST36 acupuncture was decreased, but there is no statistical. Conclusions: These results suggested that acupuncture stimulation of ST 36 has effect on ST and HR in human body, and may be used by treatment as control the nerve system.

대금음자가미와 이(梨)추출물 배합제제가 알코올로 유발된 간손상에 미치는 영향

윤대환 ( Dae Hwan Youn ),김왕인 ( Wang In Kim ),나창수 ( Chang Su Na ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.1

Objectives : The effect of pear extract with Daekumeumjagami and vitamin C medication(PDV) on alcohol metabolism and hepatic injury was assessed following hepatic injury induced by alcohol in mice. Methods : The model of alcoholic hepatic injury was established by orally administration with 3 g/kg 25% alcohol in mice. PDV was orally administrated once a day for 5 days. Mice were randomly divided into 5 groups : normal group, control group, and PDV groups (PDV-A, PDV-B and PDV-C). The activities of aspartate amino transferase (AST) and alanine amino transferase (ALT) and alcohol dehydrogenase (ADH) in serum, superoxide dismutase (SOD) and catalase in liver were determined after alcohol exposure. Results : Compared with control group, treatment with PDV-B and PDV-C significantly elevated activities of ADH. Moreover, the index of hepatic injury in serum was significantly decreased by treatment with PDV-B and PDV-C in ALT activity and PDV-C in AST activity. Additionally, enhanced catalase activities in liver was found in PDV-C treated mice after exposure to alcohol. Also, WBC in blood was significantly lower by treatment with PDV-B and PDV-C. Conclusions : This study suggests that PDV treatment could enhance alcohol metabolism, and prevent hepatic injury after alcoholic hepatic injury and that this effect is likely related to its modulation on the alcohol metabolizing and antioxidant enzymes.

저준위 레이져 요법이 고지방식이(高脂肪食餌)로 유발(誘發)된 고지혈증(高指血症),비만백서(肥滿白鼠)의 혈청지질(血淸脂質) 및 간기능(肝機能)에 미치는 영향(影響)

윤대환 ( Dae Hwan Youn ),이유경 ( Yu Kyung Lee ),최동희 ( Dong Hee Choi ),김성철 ( Sung Chul Kim ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.4

Objectives: Laser therapy started hi 1958 when Schawlow and Townes suggested medical value of Laser therapy. He-Ne laser has been utilized as a clinical treatment for various diseases by Flog since 1975. Low Level Laser Therapy (LLLT) has been used as medication for controlling obesity in the Korean Medicine. So this study is planned to investigate the effects of LLLT on the level of serum lipid and weight gain. Methods: Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and LLLT by helium-neon (He-Ne) on the tail is carried out once a 2 day during 5 weeks. The animals were divided into six groups: no ischemia-induced and no LLLT-treated group (Normal), the ischemia-induced and no LLLT-treated group (Control), the ischemia-induced and 5 mW 5 min LLLT-treated group (LLLT5-5), the ischemia-induced and 30 mW 5 min LLLT-treated group (LLL30-5), the ischemia-induced and 5 mW 10 min LLLT-treated group (LLLT5-10), 30 mW 10 min LLLT-treated group (LLLT30-10). The effect of LLLT is observed by weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Results: Body weight and food intake were decreased in LLL5-5, LLLT30-5, LLLT30-10. Food efficiency was decreased in LLLT30-10. The level of serum Triglyceride, Free fatty acid, AST, ALT, ALP were decreased in LLLT30-10. Serum HDL-cholesterol was increase in LLLT5-10, LLLT30-10. Also serum ALT was decrease in LLLT5-5. Conclusions: LLLT(30 mW-10 min) is effective on Body weight, food efficiency ratio, the level of serum lipid and protection of liver function by obesity induced by high fat diet, and LLLT(5 mW-5 min) act on decrease of Body weight, food intake and ALT.

지구자 및 인진호(茵蔯蒿) 배합(配合) Propolis의 구강투여(口腔投與)가 D-Galactosamine으로 유발(誘發)된 간손상(肝損傷)에 미치는 영향(影響)

윤대환 ( Dae Hwan Youn ),정종길 ( Jong Gil Jeong ),나창수 ( Chang Su Na ) 대한본초학회 2006 大韓本草學會誌 Vol.21 No.3