Estrogen處理 白鼠 子宮平滑筋 膜分劃의 Nitrendipine Binding Site에 關한 硏究

盧宜善,昔廷鎬,李載欣 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

Ouabain and nitrendipine binding site of uterine membrane fraction, prepared from estrogen treated rats, were compared with those prepared from control rats. Ovary was removed in control and estrogen treated rats. 1) Estrogen treatment induced increase of maximal binding site of ouabain, but decrease of affinity to binding site. 2) Nitrendipine binding site was increased significantly in uterine membrane fraction prepared from estrogen-treated rat, but affinity to binding site was not changed. From the above results, it is suggested that estrogen can increase the number of nitrendiping binding site and/or calcium channel.

일반연제 발표 : 자궁내태아발육지연(子宮內胎兒發育遲延)의 진단에 있어서 Ponderal Index의 가치

노의선,유태식,남주현,정좌구,권순욱 대한산부인과학회 1986 대한산부인과학회 학술대회 Vol.58 No.-

병합임신 1예

노의선,이광배,김현식,이용우,정좌구 대한산부인과학회 1986 Obstetrics & Gynecology Science Vol.29 No.6

선천성 복벽결손을 동반한 양막대증후군 1 예

노의선,김영석,김홍기,조용,이성원,정민영,정우철,한성태 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.3

최근 본원 산부인과에서, 배란유도에 의하여 쌍태아를 임신하고 임신 35주째에 조기진통으로 인하여 제왕절개술을 시행받은 여성에서 복벽결손과 양막대증후군을 동시에 보이는 신생아 1예를 경험하였기에 이에 대한 문헌고찰과 함께 보고하는 바이다. The amniotic band syndrome is a collection of fetal malformations associated with fibrous bands that appear to entangle or entrap various fetal parts in utero, leading to deformation, malformation, or disruption. The fibrous bands are mesodermic in origin and thought to be emanated from external surface of ruptured amniotic membrane. Misdiagnosis and inappropriate family counseling are common features. We experienced one case of amniotic band syndrome in 32 years old primigravid woman who had twin pregnancy after ovulation induction with Pergonal. We report the case with a brief review of literatures.

초음파 아두대횡경치와 대퇴골길이에 의한 태아연령에측의 정확도에 관한 통계적 비교

노의선(YS Rho),고승권(SK Koh),김현식(HS Kim),이용우(YW Lee),남주현(CH Nam),정좌구(JK Chung) 대한산부인과학회 1987 Obstetrics & Gynecology Science Vol.30 No.3

월경력이 정확한 임신 제 25주에서 42주까지의 임부 269명을 대상으로, 태아연령을 예측하기 위하여 초음파를 이용한 BPD와 FL를 측정하여 다음과 같은 결론을 얻었다 1 임신주수와의 상관관계를 비교하여 볼 때 FL(r2=0 8955)가 BPD(r2=0 8255)보다 태아연령을 예측하는 척도로서 더 유의함을 알 수 있었다 2 FL가 GA의 상관관계를 나타내는 방정식중 직선방정식(r2=0 8955, t=48 7, P<0 01) 보다는 2차방정식(r2=0 9006, t=3069, P<0 01)이 태아연령의 예측에 더 정확한 model이었다 그러나 BPD에서는 직선방정식과 2차방정식(r2=0 8273, t=1 679, P>0 05)간에 태아연령예측의 정확도에서 유의한 차가 없었다 3 FL와 BPD를 함께 조합하여 산출한 새로운 방정식은 GA=-5 77+(1 8126) FL-(0 008372) FL2-(0 7453) BPD+(0 002966) BPD2으로써 r2=0 9106이었다 즉 태아연령의 예측에 있어서 FL나 BPD를 단독으로 사용하는 것보다 둘을 조합하였을때가 더 정확한 예측이 가능함을 알 수 있었다 4 GA에 대한 logarithmic function을 도입하여 산출한 회귀방정식은 LogGA =2 0068+ (0 0576) FL-(0 000288) FL2-(0 0172) BPD+(0 000065) BPD2으로 r2=0 9285로써 태아연령예측에 가장 적합한 공식이었다 Ultrasonic measurements of biparietal diameter(BPD) and femur length(FL) for estimation of gestational age(GS) were performed in 269 pregnant women between the 25th and 42nd weeks of gestation The results were as follows: 1 Femur length(r2=0 8955) was found to provide a more accurate index than BPD(r2=0 8255) for prediction of gestational age in the linear regression equation 2 The linear quadratic function for describing the relation between FL and GA was a more accurate model than the linear function(r2=0 9006, t=3 69, P<0 01) But no significant improvement in the accuracy for predicting GA was observed using the linear quadratic function in the relation between BPD and GA(r2=0 8283, t=1 679, P>0 05) 3 Combining the FL and BPD, the the new regression equation is GA=-5 77+(1 8126) FL-(0 008372) FL2-(0 7453) BPD+(0 002966) BPD2(r2=0 9106, P<0 01) It was found that this formula provided more accurate prediction of GA than that of using either FL or BPD 4 The multiple regression of log(GA) on EL, FL2, BPD and BPD2, Log(GA)=2 0068+(0 0576) FL-(0 000288) FL2-(0 0172) BPD+(0 000065) BPD2 showed the highest coefficient of determination(r2=0 9285) So it is concluded that this is the most accurate formula for prediction of GA

양측 난관임신 1 예

권순욱,노의선,황성한,박재정,전동호 대한산부인과학회 1991 Obstetrics & Gynecology Science Vol.34 No.5

저자들은 최근 본병원 산부인과에서 양측 난관임신을 치험하였기에 문헌적 고찰과 함께 이를 보고하는 바이다. A case of bilateral tubal pregnancy which was experienced in our hospital recently, is presented with brief references.

미세수술을 이용한 난관복원술 후 임신율에 대한 고찰

김용필,노의선,조용,이성원,박주진,김경택,양승권,이면우,정석희 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.9

From Jan. 1991 to Apr. 1996, 99 cases of microsurgical tubal reversal were performed in the Department of Obstetrics and Gynecology, Chunchon Sacred Heart Hospital of Hallym Uni- versity. 80 cases among them were followed up over 1 year postoperatively. The results were as follows : 1. The mean age of total 99 women was 32.5 years(24∼42 years), and the average interval between tubal sterilization and tubal reversal was 7.2 years(1∼17 years). 2. The methods of sterilization were laparoscopic fallope ring in 59 cases(59.6 %), lapa- roscopic electrocautery in 30 cases(30.3 %), tubal ligation during cesarean section in 9 cases(9.1 %) and minilap in 1 case. 3. The most common reason for tubal reversal was remarriage(61 case, 61.6 %) and the next was desire for son(16 cases, 16.2 %). 4. 80 cases(81 %) among the total 99 women were followed up over 1 year postope- ratively. Intrauterine pregnancy was documented in 59 cases(73.7 %), 53 viable fetuses and 6 spontaneous abortions. Ectopic pregnancy was noted in 3 cases(3.8 %). 5. Pregnancy rates according to maternal age were as follows ; 88.9 % in less than 30 years, 70.3 % in 30∼34 years, 71.4 % in 35∼39 years. Two normal pregnancies were resulted from 4 women over 39 years. 6. Pregnancy rates according to the method of sterilization were as follows ; 78 % in fallope ring, 89 % in tubal ligation during cesarean section, 55 % in electrocautery. The statistical diffe- rences between each groups were not significant(p = 0.053). 7. Pregnancy rates according to the site of anastomosis were as follows ; 81.4 % in isthmi- cisthmic, 67.1 % in isthmic-ampulla. There was significant statistical difference between them(p = 0.011). 8. Pregnancy rates according to the postoperative tubal length were as follows ; 60 % in less than 4cm, 66 % in 4∼6 cm, 95.4 % in more than 6cm(p = 0.024). 9. Among the 59 pregnant women, 38 pregnancies(65.4 %) were resulted within six months after surgery, 48(82.3 %) within one year, and 55(94.2 %) within 2 years. 10. Pregnancy rates according to the interval from tubal sterilization to tubal reversal were as follows ; 89.6 % in less than 5years of interval, 74.5 % in 5∼10 years, 44.4 % in 11∼15 years(p = 0.230).

Transferrin Receptors in the Liver Cell Membrane of Carcinogen (3-methyl-4-dimethyl-arninoazobenzene) Treated Rat

이재흔,노의선,허강민,이충식,석정호,Lee, Jae-Heun,Ro, Eu-Sun,Hur, Gang-Min,Lee, Choong-Sik,Seok, Jeong-Ho The Korean Society of Pharmacology 1993 대한약리학잡지 Vol.29 No.1

화학물질에 의한 간암 유발과정에서 transferrin receptor (TfR)의 변동을 밝히기 위해 간을 부분절제한 정상백서의 재생간과 발암물질로 3-Me-DAB를 8주간 투여한 백서 또는 약물 투여 후 부분 간절제 수술을 행하여 세포분열을 유도시킨 백서 간조직으로부터 parenchymal cell (PC)과 nonparenchymal cell (NPC)를 분리하고 각각의 세포막을 제조하여 $^{125}I-transferrin$ 결합실험을 실시한 바 다음과 같은 성적을 얻었다. 1. 3-Me-DAB 투여에 의하여 간조직에서 oval cell의 증식, 재생성 변화, 결절형성, 담관의 증식 및 담관세포암 등의 현저한 조직학적 변화가 동반되었다. 그러나 간세포증식을 더욱 촉진시키기 위하여 부분간절제 수술을 하였을 때 수술 후 경과에 따른 형태학적 변동은 큰 차이가 없었다. 2. 정상 재생간의 PC 및 NPC homogenate에서 transferrin 결합량은 부분간 절제 수술 후 1일 및 3일에 증가되었으며 수술 후 7일에 정상으로 회복되었다. 3-Me-DAB 투여에 의해 두세포군에서 모두 정상세포보다 높게 나타났으며 재생기간에 따라 계속 증가되었다. 3. 정상간의 NPC 세포막에서 transferrin 최대 결합량 (Bmax)은 PC 세포막에서 보다 많이 분포되어 있었으며, Kd는 양세포막에서 5.05 또는 6.3nM로 비슷하였다. 4. 재생간의 NPC 및 PC 세포막에서 transferrin 결합량은 부분 간절제 수술 후 1일 및 3일에 $40{\sim}50%$ 증가되었고 수술 후 7일에 정상치로 회복되었다. 5. 3-Me-DAB 처치에 의하여 NPC 및 PC 세포막의 transferrin 결합량은 정상 간세포막에서 보다 약 3배 증가되었고, 3-Me-DAB 투여후 재생간의 NPC 세포막에서는 부분 간절제 수술 후 3일까지 증가된 후 감소되는 양상인데 반해 PC 세포막에서는 수술 후 7일까지 계속 증가되었다. 6. 3-Me-DAB 투여 후 NPC 및 PC 세포막 transferrin binding site에서 Kd치가 $3.1{\sim}4.1\;nM$과 $25.4{\sim}54.1\;nM$인 두 종류가 존재하는 것으로 나타났다. 이상의 실험성적으로 TfR는 1) 간조직의 PC 및 NPC 세포에 모두 분포되어 있으며, 2) 정상 재생간 및 3-Me-DAB의 처리 후 간세포에서의 세포막 TfR의 증가는 세포내 합성량의 증가에 의하여 일어나며, 3) 정상 재생간의 세포막 TfR는 한 종류의 high affinity site $(Kd,\;<3.1{\sim}7.5\;nM)$에 의하여 증가되나, 3-Me-DAB 처리 후 간세포막에서는 정상에서와 같은 high affinity형 이외에 affinty가 낮은 다른 형태의 TfR $(Kd,\;25.4{\sim}54.1\;nM)$가 세포막으로 출현됨으로써 크게 증가되는 것으로 사료된다. To investigate the alteration of transferrin receptor (TfR) in the proliferating or transformed liver cells, $^{125}I-transferrin$ binding experiment was carried out in the isolated parenchymal cells (PC) or nonparenchymal cells (NPC) from normal regenerated rat liver after partial hepatectomy and from the liver of 3-methyl-4-dimethyl-aminoazobenzene (3-Me-DAB) treated rat. With the administration of 3-Me-DAB for 8 weeks, the liver tissue showed marked morphologic changes of oval cell proliferation, regenerations of hepatocytes, and atypical proliferations of bile ducts, but these changes were little affected by partial hepatectomy. Transferrin binding values in PC or NPC homogenate from the regenerated liver of normal rat, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With the treatement of 3-Me-DAB for 8 weeks, transferrin binding sites in homogenates were higher than those of normal rat liver and increased by 7th day after partial hepatectomy. Transferrin binding sites (Bmax) in the cell membrane of NPC were higher than those of PC of normal rat liver, but there was no significant difference in Kd values between both groups (5.05, 6.3 nM). In the normal resenerated rat liver, transferrin binding sites in the PC or NPC plasma membrane, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With 3-Me-DAB tratment, transferrin binding sites in both liver NPC and PC plasma membrane were increased about 3 folds, compared to those in each plasma membrane of normal rat liver. And after partial hepatectomy of 3-Me-DAB trated rat, transferrin binding sites were increased by the 3rd day in the NPC plasma membrane but increased by the 7th day in the PC plasma membrane. In the transferrin binding sites of the PC or NPC plasma membrane of 3-Me-DAB treated liver, two kinds of Kd values $(3.1{\sim}4.7\;nM,\;25.4{\sim}54.1\;nM)$ were detected. The present results suggest that 1) TfRs are distributed in the liver PC as well as NPC; 2) Increased TfRs in PC or NPC plasma membrane of normal regenerated liver after partial hepatectomy and 3-Me-DAB treated rat liver, may be due to increased intracellular synthesis; 3) Increased TfRs in normal regenerated liver after partial hepatectomy might be related to the expression of a single type of high affinity site $(Kd,\;3.1{\sim}7.5\;nM)$, but in 3-Me-DAB treated rat liver might be related to the expression of high and low affinity types of receptors $(Kd,\;25.4{\sim}54.1\;nm)$.

Carcinogen (3-methyl-4-dimethyl-aminoazo benzene) 처리후 간세포막에서의 Transferrin Receptor 변동에 관한 연구

이재흔,노의선,허강민,이충식,석정호 大韓藥理學會 1993 대한약리학잡지 Vol.29 No.1

화학물질에 의한 간암 유발과정에서 transferrin receptor (TfR)의 변동을 밝히기 위해 간을 부분절제한 정상백서의 재생간과 발암물질로 3-Me-DAB를 8주간 투여한 백서 또는 약물 투여 후 부분 간절제 수술을 행하여 세포분열을 유도시킨 백서 간조직으로부터 parenchymal cell (PC)과 nonparenchymal cell (NPC)를 분리하고 각각의 세포막을 제조하여 <TEX>$^{125}I-transferrin$</TEX> 결합실험을 실시한 바 다음과 같은 성적을 얻었다. 1. 3-Me-DAB 투여에 의하여 간조직에서 oval cell의 증식, 재생성 변화, 결절형성, 담관의 증식 및 담관세포암 등의 현저한 조직학적 변화가 동반되었다. 그러나 간세포증식을 더욱 촉진시키기 위하여 부분간절제 수술을 하였을 때 수술 후 경과에 따른 형태학적 변동은 큰 차이가 없었다. 2. 정상 재생간의 PC 및 NPC homogenate에서 transferrin 결합량은 부분간 절제 수술 후 1일 및 3일에 증가되었으며 수술 후 7일에 정상으로 회복되었다. 3-Me-DAB 투여에 의해 두세포군에서 모두 정상세포보다 높게 나타났으며 재생기간에 따라 계속 증가되었다. 3. 정상간의 NPC 세포막에서 transferrin 최대 결합량 (Bmax)은 PC 세포막에서 보다 많이 분포되어 있었으며, Kd는 양세포막에서 5.05 또는 6.3nM로 비슷하였다. 4. 재생간의 NPC 및 PC 세포막에서 transferrin 결합량은 부분 간절제 수술 후 1일 및 3일에 <TEX>$40{\sim}50%$</TEX> 증가되었고 수술 후 7일에 정상치로 회복되었다. 5. 3-Me-DAB 처치에 의하여 NPC 및 PC 세포막의 transferrin 결합량은 정상 간세포막에서 보다 약 3배 증가되었고, 3-Me-DAB 투여후 재생간의 NPC 세포막에서는 부분 간절제 수술 후 3일까지 증가된 후 감소되는 양상인데 반해 PC 세포막에서는 수술 후 7일까지 계속 증가되었다. 6. 3-Me-DAB 투여 후 NPC 및 PC 세포막 transferrin binding site에서 Kd치가 <TEX>$3.1{\sim}4.1\;nM$</TEX>과 <TEX>$25.4{\sim}54.1\;nM$</TEX>인 두 종류가 존재하는 것으로 나타났다. 이상의 실험성적으로 TfR는 1) 간조직의 PC 및 NPC 세포에 모두 분포되어 있으며, 2) 정상 재생간 및 3-Me-DAB의 처리 후 간세포에서의 세포막 TfR의 증가는 세포내 합성량의 증가에 의하여 일어나며, 3) 정상 재생간의 세포막 TfR는 한 종류의 high affinity site <TEX>$(Kd,\;<3.1{\sim}7.5\;nM)$</TEX>에 의하여 증가되나, 3-Me-DAB 처리 후 간세포막에서 To investigate the alteration of transferrin receptor (TfR) in the proliferating or transformed liver cells, <TEX>$^{125}I-transferrin$</TEX> binding experiment was carried out in the isolated parenchymal cells (PC) or nonparenchymal cells (NPC) from normal regenerated rat liver after partial hepatectomy and from the liver of 3-methyl-4-dimethyl-aminoazobenzene (3-Me-DAB) treated rat. With the administration of 3-Me-DAB for 8 weeks, the liver tissue showed marked morphologic changes of oval cell proliferation, regenerations of hepatocytes, and atypical proliferations of bile ducts, but these changes were little affected by partial hepatectomy. Transferrin binding values in PC or NPC homogenate from the regenerated liver of normal rat, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With the treatement of 3-Me-DAB for 8 weeks, transferrin binding sites in homogenates were higher than those of normal rat liver and increased by 7th day after partial hepatectomy. Transferrin binding sites (Bmax) in the cell membrane of NPC were higher than those of PC of normal rat liver, but there was no significant difference in Kd values between both groups (5.05, 6.3 nM). In the normal resenerated rat liver, transferrin binding sites in the PC or NPC plasma membrane, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With 3-Me-DAB tratment, transferrin binding sites in both liver NPC and PC plasma membrane were increased about 3 folds, compared to those in each plasma membrane of normal rat liver. And after partial hepatectomy of 3-Me-DAB trated rat, transferrin binding sites were increased by the 3rd day in the NPC plasma membrane but increased by the 7th day in the PC plasma membrane. In the transferrin binding sites of the PC or NPC plasma membrane of 3-Me-DAB treated liver, two kinds of Kd values <TEX>$(3.1{\sim}4.7\;nM,\;25.4{\sim}54.1\;nM)$</TEX> were detected. The present results sug

병합임신 1례

이지연,노의선,김이규,정좌구,오원섭 한림대학교의료원 1984 人間科學 Vol.8 No.12