19-norandrostenedione이 흰쥐 정소내 스테로이드 대사에 미치는 영향

김정욱,윤용달,Kim, Jeong-Wook,Yoon, Yong-Dal 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.3

19-norandrostenedione(19-NORA) is known as an intermediate in the metabolic pathway from androstenedione to estrone. Administration of esterified 19-nortestosterone, anabolic steroid, reduces serum gonadotropin and testosterone concentration, and results in reversible azoospermia in men. 19-NORA have been isolated from testis, but its function in testis is not clear yet. Therefore, this study was designed to determine the effect of 19-NORA on steroidogenesis and on spermatogenesis. 19-NORA was administrated by single intratesticular injection to adult male rats weighing 350-400 g in dose of 1 mg/50${\mu}l$. The serum and testis were collected on 1, 3, 7, 12, 48 hr after injection. The histological differences in testis were observed by routine paraffin method. The concentrations of testosterone and estradiol in serum and in left testis were determined by the conventional radioimmunoassays. One hour after 19-NORA treatment, serum concentrations of testosterone and estradiol increased significantly, compared to those of pre-treated(0 hr) group, and reduced gradually to the control level on 7 hour after injection. The concentration of testosterone in left testis increased slightly 1 hour after injection, and estradiol level increased significantly(p<0.05). Also, testosterone and estradiol level of control group revealed no difference with pre-treated (0 hr) group. Gonad index, structure of seminiferous tubules, and the number of step 7 th spermatid were simillar to control group. The present study suggests that the elevation of testosterone level results from increment of estradiol followed by the rapid metabolism of 19-NORA at 7 hour after injection, and then testosterone concentration may be recovered to control level by feedback mechanism of hypothalamus-hypothysis-testis axis.

자연발생석면 분포지역의 효율적인 조사와 관리 방안

김정욱,정명채,김동진,전기석,이창환,Kim, Jeong-Wook,Jung, Myung-Chae,Kim, Dong-Jin,Jeon, Gee-Seok,Lee, Chang-Hwan 한국지하수토양환경학회 2011 지하수토양환경 Vol.16 No.6

This study presents a state of the art in evaluation on naturally occurring asbestos (NOA) areas and effective strategies for investigation and management of NOA areas in Korea. First of all, we defined and classified the NOA area as compiling various countries' regulations, then discussed the best methods for evaluation and management of NOA for reducing exposure of asbestos in the vicinity of NOA areas. According to the literature survey, a three-steps management strategy was suggested in this study. The first step is a confirmation stage of asbestos contamination possibility and the second step is an assessment stage of asbestos contamination using investigation, analysis, evaluation and reclamation. The final step is a following-up control stage. These three management steps for NOA area will give the maximum effectiveness of NOA control in Korea.

강섬유 및 유기섬유를 혼입한 콘크리트 및 모르타르의 내충격 성능평가

김정욱,김규용,Miyauchi Hiroyuki,남정수,김홍섭 한국구조물진단유지관리학회 2011 한국구조물진단학회 학술발표회논문집 Vol.2011 No.9

In this study, it evaluate impact resistance performance of steel fiber and organic fiber reinforced concrete and mortar. The type of organic is polypropylene and polyvinyl alcohol. Percentage of fiber is 0.1, 0.5, 1.0%. It evaluate impact resistance performance to projectile 7, 10㎜ size and 350㎧ by high speed impact testing device.

위팔두갈래근의 긴갈래와 짧은갈래의 팔굽관절 굽힘 각도에 따른 근활성도 분석

김정욱,박민철,Kim, Jeong-Wook,Park, Min-Chull 대한고유수용성신경근촉진법학회 2020 PNF and Movement Vol.18 No.1

Purpose: This study aimed to investigate the impact of resistance on the muscle activities of the long and short heads of the biceps brachii, according to the elbow angle in supination, and the difference in muscle activity between the long and the short heads. Methods: This study was conducted with 22 men in their 20s who voluntarily agreed to participate. With the glenohumeral joint neutral in a position of supination, the elbow angle was randomly moved to 0°, 30°, 60°, and 90°. Using an 8-channel surface EMG while the participants held a 2-kg. dumbbell, the muscle activities of the long and the short heads of the biceps brachii were measured. The measured data were statistically processed using SPSS for Windows 12.0. For the activities of the short and the long heads according to the angle, a one-way ANOVA was conducted, and subsequently, to check the results of an analysis of the difference between groups, an LSD post-hoc test was conducted. An independent t-test was used to compare the activities of the long head and the short head according to the angles. Results: The analysis of the impact of the load of the dumbbell at each elbow angle on the muscle activities of the long and short heads of the biceps brachii revealed significant differences in both heads (p < 0.05). The result of the post-hoc analysis showed significant differences in both heads at angles between 0° and 30°, between 0° and 60°, and between 0° and 90°. Analysis of the impact of the load of the dumbbell in supination on the muscle activities of the long and short heads showed a significant difference at the angle between 0° and 30° (p < 0.05). Conclusion: The long head of the biceps brachii mainly acts in supination; however, in supination at elbow-bending angles of 60° and 90°, there was no difference in muscle activity between the short head and the long head.

일반적인 체외수정 방법과 세포질내 정자주입술로 얻어진 배아의 동결-융해 후 이식의 결과

김정욱,한미현,변혜경,전진현,손일표,궁미경,백은찬,강인수,이호준,Kim, Jeong-Wook,Han, Mi-Hyun,Byun, Hye-Kyung,Jun, Jin-Hyun,Son, Il-Pyo,Koong, Mi-Kyoung,Paik, Eun-Chan,Kang, Inn-Soo,Lee, Ho-Joon 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.1

Intracytoplasmic sperm injection (ICSI) recently has been utilized widely as the most successful technique to overcome the unfertilization problem in cases of severe male infertility in couples who could not be treated by conventional IVF. Recently, indications of ICSI have been extended further and more fertilized oocytes become available. Thus, it is necessary to examine the efficiency of freezing the surplus embryos obtained from ICSI. We compared the survival rate and the future outcome of cryopreserved embryos obtained either after conventional IVF or ICSI during the same period. After ICSI or IVF, five best-quality embryos from each patient were transferred in the stimulation cycle and the surplus pronuclear (PN) stage oocytes or multicellular embryos were cryopreserved by slow freezing protocol with 1,2-propanediol (PROH) as a cryoprotectant. A total of 792 embryos from ICSI trial were thawed and 65.2% (516/792) survived. The survival rates of PN stage oocyte, multicellular embryo and PN + multicellular embryo were 63.5%, 68.2%, 64.0%, respectively. After 111 transfers, 34 pregnancies were achieved, corresponding to a clinical pregnancy rate of 30.6% per transfers. We thawed 1033 embryos from IVF trials and 57.5% (594/1033) survived. In IVF cycle, the survival rates of PN stage oocyte, multicellular embryo and PN + multicellular embryo were 58.2%, 65.2%, 40.2%, respectively. Thirty eight clinical pregnancies were established after 134 transfers, corresponding to a pregnancy rate of 28.4% per transfer. The cleavage rate of thawed PN stage oocytes from ICSI trial (61.3%) was significantly higher than those from conventional IVF (53.4%). The developmental rates of good embryo (${\geqq}$ grade II) in thawed PN stage oocytes obtained from conventional IVF and ICSI were 63% and 65%, respectively. We concluded that PN stage oocytes, multicellular embryos resulting from ICSI procedure can be successfully frozen/thawed with reasonable clinical pregnancy rates comparable to those of IVF.

동결수정란 이식주기에서 수정란 융해 후 생존율과 임신율에 영향을 미치는 요인

김정욱,변혜경,염혜원,전진현,박용석,송인옥,송지홍,최범채,궁미경,전종영,강인수,Kim, Jeong-Wook,Byun, Hye-Kyung,Youm, Hye-Won,Jun, Jin-Hyun,Park, Yong-Seog,Song, In-Ok,Song, Ji-Hong,Choi, Bum-Chae,Koong, Mi-Kyoung,Jun, Jong-Young,Kang, 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

Objective: The purpose of this study was to determine the important factors affecting survival and pregnancy rate in frozen-thawed embryo transfer cycles. Methods: we performed retrospective analysis in 738 cycles of frozen-thawed embryo transfers, in relation to the insemination methods, the freezing stage of embryo, patient's age, infertility factors and the origin of injected sperm in ICSI cycles. After conventional IVF or ICSI, the supernumerary PN stage zygotes or multicellular embryos were cryopreserved by slow freezing protocol with 1,2-propanediol (PROH) as a cryoprotectant. Results: The survival rates of thawed embryos were 69.3% (1585/2287) in conventional IVF group and 71.7% (1645/2295) in ICSI group. After frozen-thawed embryo transfers, 27.0% (92/341) and 32.0% (109/341) of pregnancy rates were achieved in conventional IVF and ICSI group, respectively. There were no significant difference in the survival and pregnancy rates according to the insemination methods, the freezing stage and patient's age. However, the pregnancy rate (36.2%) of male factor infertility was significantly higher than the tubal (27.2%) and other female factor infertility (22.9%). In ICSI group, the origin of injected sperm did not affect the outcome of frozen-thawed embryo transfer cycles. Conclusion: The present study demonstrates that acceptable clinical outcomes can be achieved after the transfer of frozen-thawed embryos regardless of the stage of embryos for freezing, the patient's age and the origin of injected sperm.

식도암의 외과적 치료성적

김정욱(Jeong Wook Kim),김충배(Choong Bae Kim),민진식(Jin Sik Min) 대한외과학회 1998 Annals of Surgical Treatment and Research Vol.54 No.6

Diclofenac과 methotrexate 병합 경구 투여한 백서에서의 급성 장관손상과 장내세균전위

김정욱 ( Jeong Wook Kim ) 대한내과학회 2007 대한내과학회지 Vol.73 No.3

목적: 비스테로이드성 항염제와 methotrexate는 전장관에 걸쳐 손상을 일으키고 감염성 합병증인 장내세균전위를 유발한다. 하지만 비스테로이드성 항염제의 장관손상과 장내세균전위에 대한 methotrexate의 영향에 대해서는 아직 알려진 바가 없다. 이번 연구에서는 실험동물에서 비스테로이드성 항염제의 장관손상과 장내세균전위에 대한 methotrexate의 영향을 알아보았다. 방법: 백서를 정상 대조군, methotrexate 단독 투여군, diclofenac 단독 투여군, 그리고 methotrexate와 diclofenac 병합투여군으로 나누고 methotrexate는 20 mg/kg 용량으로 일회 경구투여하고 diclofenac은 80 mg/kg 및 120 mg/kg로 일회 경구투여 하였다. 약물 투여 2일 후 장관손상을 알아보기 위하여 20시간 동안 장투과성검사와 배변횟수를 측정한 후 장관유착도도 관찰하였다. 원위부 회장과 맹장에서 호기성 및 그람음성 장내세균수와 장간막 림프절, 간, 비장, 신장과 심장에서의 그람음성균수를 측정하였으며, 실험기간 동안 식이량과 체중변화도 관찰하였다. 결과: Diclofenac이나 methotrexate 단독 투여에 의하여 장투과성과 장관유착도가 증가하고 배변횟수가 감소하였으며 장내세균의 과증식와 장내세균전위가 발생하였다. Diclofenac과 methotrexate 병합투여군에서는 diclofenac이나 methotrexate 단독 투여군보다 장투과성과 장관유착도가 증가하고 배변횟수가 감소하였다. 또한 diclofenac 80 mg/kg 투여에서 diclofenac 단독투여군보다 그람 음성 장내세균수가 감소하였고, diclofenac의 투여용량과 상관없이 간, 비장, 신장과 심장에서 장내세균전위가 감소하였다. 식이투여량과 체중감소는 연구 결과에 영향이 없었다. 결론: 백서에서 methotrexate는 비스테로이드성 항염제에 의한 장관손상을 증가시키지만 간, 비장, 신장과 심장에서의 장내세균전위는 감소시킨다. Background: NSAIDs and methotrexate induce gut damage and bacterial translocation (BT). However, there is no study examining the combined effects of methotrexate and NSAID on gut damage and BT. We examined the combined effects of methotrexate and NSAID-induced enteropathy and bacterial translocation in an experimental animal model. Methods: Rats received either no drug, NSAID alone (diclofenac 80 mg/kg and 120 mg/kg per os), methorexate alone (20 mg/kg per os) or NSAID with methotrexate. Gut barrier dysfunction, the degree of intestinal adhesion, stool pellet number, bacterial number of total aerobes and Gram negatives in the distal ileal and cecal contents and the number of Gram negatives in the mesenteric lymph nodes, liver, spleen, kidney and heart were measured. Results: Administration of diclofenac or methotrexate alone caused an increase in gut barrier dysfunction and intestinal adhesion and a decrease in stool pellet number. Administration of diclonfenac alone induced enteric bacterial overgrowth and increased BT to the mesenteric lymph nodes, liver, spleen, kidney and heart. Administration of methotrexate alone induced enteric bacterial undergrowth and BT to the mesenteric lymph nodes, liver, spleen but not to the kidney and heart. The supplements with methotrexate increased the NSAID-induced gut barrier dysfunction and intestinal adhesion, and decreased the stool pellet number. However, the reduced NSAID-induced enteric Gram negative bacterial overgrowth (with a dose of diclofenac of 80 mg/kg) and BT to the liver, spleen, kidney and heart. Conclusion: Methotrexate increases NSAID-induced intestinal damage, but reduces NSAID-induced BT to the liver, spleen, kidney and heart in experimental animals.(Korean J Med 73:258-266, 2007)

폐색성 담관질환자의 장투과성의 변화

김정욱 ( Jeong Wook Kim ),전우규 ( Woo Kyu Jeon ),장세경 ( Sae Kyung Chang ),이정안 ( Jung Ahn Lee ),박동일 ( Dong Il Park ),조용균 ( Yong Kyun Cho ),성인경 ( In Kyung Sung ),손정일 ( Chung Il Sohn ),김병익 ( Byung Ik Kim ),김은정 대한내과학회 2004 대한내과학회지 Vol.67 No.6

목적 : 장관장벽 이상은 폐색성 담관질환에서 발생하며 장내세균전위나 장관내독소혈증과 같은 감염성 합병증을 유발 한다. 본 연구에서는 폐색성 담관질환에서 장관장벽 이상과 임상양상과의 관계를 알아보았다. 방법 : 건강대조군 18명, 양성질환에 의한 폐색성 담관질환자 20명, 악성질환에 의한 폐색성 담관질환자 21명에서 장투과성 검사를 실시하였으며 질환군간 차이와 각종 임상양상과 연관성을 비교하였다. 장투과성 검사는 51Cr-EDTA (51Cr-ethylen Background : Gut barrier dysfunction occurs in experimental models and humans of obstructive biliary disease. This phenomenon promotes infectious complications including bacterial translocation and intestinal endotoxemia. The aims of this study were to ex

Enterococcus faecium 함유 프로바이오틱스 복용자에서 발생한 Enterococcus faecium 유발 원내 감염증의 항생제 내성 양상

김정욱 ( Jeong Wook Kim ) 한국정맥경장영양학회 2007 한국정맥경장영양학회지 Vol.1 No.1