Development of Molecular Markers for Bentazone-Resistant Wheat (Triticum aestivum L.) Induced by Gamma-ray Irradiation

김재윤,서용원,장철성,D. S. Kim,김대연,J. B. Kim,K-R Kim 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.I

Wheat mutants generated from a cultivar ``Geumgangmil"by using a $^{60}$Co source (gamma-ray) were developed for bentazone (3-isopropyl-2,1,3-benzo-thiadiazin-4-one-2,2-dioxide) resistance. Resistant M$_2$ plants were selected from seedlings and adult plants consecutively. Bentazone-resistant wheat plants were able to survive at up to 10 times (1,600 ppm) the commercially recommended concentration. The four plants with the highest bentazone resistance were selected. An AFLP (amplified fragment length polymorphism) analysis was applied to identify molecular markers for bentazone-resistant wheat mutants. Twelve polymorphic products, which were unique to bentazone-resistant plants, were identified, cloned, and sequenced. Among the twelve polymorphic bands, eight Sequence-tagged Sites (STS) primer sets were designed. Only one STS primer set (HRMW-08) was converted a ``Bentazone-resistant wheat-specific" STS marker. The wheat mutants and markers that we obtained could be employed in a cross-breeding program incorporated with marker assisted selection (MAS) in the early stages of population segregation.

직접 산화법에 의한 경사기능 재료의 제조에 관한 연구

김재윤,김경식,김석윤 ( J . Y . Kim,K . S . Kim,S . Y . Kim ) 한국열처리공학회 1996 熱處理工學會誌 Vol.9 No.4

The direct reaction method has been used for the fabrication of Al-Mg/Al₂O₃functionally gradient materials. It was found that the reaction layer of the Al-Mg/Al₂O₃powder compact at 900℃ under air atmosphere led to the formation of reaction layers with varying ceramic phase contents. As the results of experiments by using the TGA system, the characteristics and growth behavior of the reaction layers were affected by the reaction temperature, the gas flow rate, the Mg contents and the Al₂O₃contents.

우리나라 청소년 음주의 사회경제적 비용 추계

김재윤,정우진,이선미,박종연,Kim, Jae-Yeun,Chung, Woo-Jin,Lee, Sun-Mi,Park, Chong-Yon 대한예방의학회 2010 예방의학회지 Vol.43 No.4

The purpose of this study was to estimate the socioeconomic costs resulting from alcohol drinking among adolescents as of 2006 from a societal perspective. Methods: The costs were classified into direct costs, indirect costs, and other costs. The direct costs consisted of direct medical costs and direct non-medical costs. The indirect costs were computed by future income losses from premature death, productivity losses from using medical services and reduction of productivity from drinking and hangover. The other costs consisted of property damage, public administrative expenses, and traffic accident compensation. Results: The socioeconomic costs of alcohol drinking among adolescents as of 2006 were estimated to be 387.5 billion won (0.05% of GDP). In the case of the former, the amount included 48.25% for reduction of productivity from drinking and hangover, 39.38% for future income losses from premature death, and 6.71% for hangover costs. Conclusions: The results showed that the socioeconomic costs of alcohol drinking among adolescents in Korea were a serious as compared with that of the United States. Therefore, the active interventions such as a surveillance system and a prevention program to control adolescents drinking by government and preventive medicine specialist are needed.

기계연구원의 미래기술 기획 및 연구현황

김재윤,박주형,김완두,Kim, Jae-Yun,Park, Ju-Hyeong,Kim, Wan-Du 재료연구소 2006 機械와 材料 Vol.18 No.3

원적외선의 인체작용메카니즘

김재윤,박영한,박돈목,박래준,Kim, Jae-Yoon,Park, Young-Han,Park, Don-Mork,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.2

Until now, it has not been well known for Far-infrared(FIR) how to affect to the human body. We introduced and presumed the mechanism of FIR based on molecular biology in this study, as below. The human body is composed of proteins which get easily changed by a thermal factor (about 42 $^{\circ}$C over). FIR with low temperature can deeply penetrate on the human body composed things without troublesome, since FIR has effectively operated on the human body at low temperature (35-40 $^{\circ}$C). When FIR penetrated on the human body, it would inhibit the abnormal genes and cells expression, and then information of DNA and RNA would be reexpressed for arranging DNA and RNA abnormal state. As FIR's receptors in the body, it colud be presumed that N-glycosyl linkage of purine and deoxyribose, RNA splicing process, and heat shock protein.

한국출판, 무엇이 문제인가

김재윤,유환옥,이창경,표정훈,Kim, Jae-Yun,Yu, Hwan-Ok,Lee, Chang-Gyeong,Pyo, Jeong-Hun 대한출판문화협회 2001 출판저널 Vol.293 No.-

콜로이드 양자점 기반 백색 발광다이오드 연구동향

김재윤,곽정훈,Kim, Jae-Yun,Gwak, Jeong-Hun 한국전기전자재료학회 2016 전기전자재료 Vol.29 No.5

원적외선이 흰쥐의 생존율에 미치는 영향

김재윤,박승규,김진상,박래준,Kim, Jae-Yoon,Park, Seung-Kyu,Kim, Jin-Sang,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.3

IR classified by wavelength three parts NIR, MIR. FIR. There is FIR which is radiated from healthy human body the wave length is 8-14m. The Sun's ray is composed of Infared(49%), Visible light(40%) and Ultra violet(11%), however the ray getting to the earth is FIR(60%), IR(20%), and UV(20%). Human beings has utilized FIR already from time immemorial. Hershel found out Infrared for the first time, in the Industrial Revolution the Infrared and FIR had been begun to use making products. FIR with low temperature can deeply penetrate on the human body composed things without troublesome, since FIR has effectively operated on the human body at low temperature (35-40 $^{\circ}$C). In this study, we experimented in the specific temperature FlR radiation intensity. water consumption rate, feed consumption rate. survival rate and mean of weight balance with FlR radiation instrument. According to the results, the FlR radiation to the mice assisted to increase the survival rate.

A New Record of the Genus Ectopsocus McLachlan, 1899 (Psocodea: Ectopsocidae) from South Korea

김재윤,홍기정,이원훈,Jaeyun Kim,Ki-Jeong Hong,Wonhoon Lee Korean Society of Applied Entomology 2023 한국응용곤충학회지 Vol.62 No.2

The genus Ecopsocus is recorded from Korea for the first time with E. briggsi McLachlen, 1899. This species was collected on southern regions, Geoje-si, Jinju-si, Ulsan-si, and Jeju-do, in Korea. In this study, illustration of diagnostic characters of E. briggsi is provided.

국내 밀 43개 품종에 대한 아그로박테리움 형질전환 효율성 검정

김재윤,이건희,이하늘,현도윤,Jae Yoon Kim,Geon Hee Lee,Ha Neul Lee,Do Yoon Hyun 국립한국농수산대학교 교육개발센터 2024 현장농업연구지 = Journal of practical agricultural resear Vol.25 No.4

본 연구는 국내 밀 43 품종에 대한 A. tumefaciens 형질 전환 효율을 검정하기 위해 GUS staining 분석을 수행한 것으로서 대부분의 밀 형질전환 연구가 특정 품종에 국한되어 있기 때문에 국내 장려 밀 품종에 대한 형질전환 효율에 영향을 미치는 조건에 대한 검정이 필요하다. 국내 밀 품종 중 32개에서 1개 이상의 조직에 염색된 신호가 관찰되었으며 4개 품종에서는 염색된 신호가 관찰되지 않았고 6개 품종에서 과도한 A. tumefaciens 성장이 관찰되었고, 7개 품종은 미성숙배의 크기 등의 이유로 GUS staining 분석이 불가능하여 추가 분석에서 제외하였다. 국내 밀 품종별 A. tumefaciens 감염 효율 비교 결과, 백립계 8개 품종 및 적립계 28개 품종에서는 평균적으로 유의한 차이가 없었다. 특히 조농밀, 조품밀, 새올밀, 조중밀, 새금강밀 등 적립계 품종에서 90% 이상의 높은 감염 효율이 관찰되었고, 전체 품종 중 22개는 50% 이상의 효율을 나타내었다. 본 연구를 통하여 조농밀 및 새올밀은 미성숙배에서 전체 조직에서 강한 GUS 발현으로 형질전환에 적합한 품종으로 나타났으며 금강 품종은 백립계에서 상대적으로 높은 발현을 보여 A. tumefaciens을 이용한 형질전환에 적합한 것으로 확인되었다. 추가적인 연구를 통해 품종의 조직배양 효율을 검정하고, 안정적인 GUS 발현 효율을 조사하는 것이 필요하며, 이를 통해 밀 형질전환에 이용 가능한 품종을 더욱 정밀하게 선발할 수 있을 것으로 판단된다. 해당 연구 결과는 국내 밀품종의 형질전환 효율을 높이기 위한 기초 자료로 활용 가능할 것이다. Agrobacterium-mediated transformation (AMT) is a method that allows for the stable integration of DNA fragments into the plant genome. Transgenic plants generated through AMT typically exhibit a lower copy number of the transgene compared to those induced by particle bombardment. Furthermore, AMT offers a straightforward and efficient approach for generating transgenic plants. While the transformation efficiency of wheat is comparatively lower than that of other monocot plants such as Rice (Oryza sativa L.) and Maize (Zea mays L.), the cultivars 'Bobwhites' and 'Fielder' are commonly employed for wheat transformation. To date, there have been no reported instances of successful development of transgenic plants using Korean wheat varieties through AMT. This study aims to assess the transformation efficiency of 43 Korean wheat cultivars using the GUS assay, with the goal of identifying suitable Korean wheat cultivars for AMT. The pCAMBIA1301 vector, carrying the β-glucuronidase (GUS) gene, was incorporated into Agrobacterium strain EH105. Following the inoculation of Agrobacterium into immature embryos, GUS assays were conducted 'Saeol', 'Jopum', and 'Jonong' showed 100% (the number of embryos showing GUS spots/the number of embryos used for AMT) among 43 cultivars. In addition, cultivars with more than 70% were 'Saekeumgang', 'Jojung', 'Tapdong', 'Anbaek', 'Dabun', 'Sugang', 'Keumgang', 'Jeokjung', 'Seodun', 'Joeun', 'Dajung', and 'Baekjung'. It seems that the 15 cultivars above showed the possibility of using AMT. On the other hand, 'Yeonbaek', 'Goso', 'Baekgang', and 'Johan' showed less than 20% and GUS spots were not observed in 'Gru', 'Gobun', 'Milseong', and 'Shinmichal-1'. This study explores transient GUS expression in Korean wheat cultivars seven days after AMT. The observed initial high efficiency of transient transformation suggests the potential for subsequent stable transformation efficiency. Korean wheat cultivars demonstrating elevated transient transformation efficiency could serve as promising candidates for the development of stable transgenic wheat.