SC-FDMA Uplink 용 직렬 간섭제거기의 성능 고찰

권혁찬(Hyuck-Chan Kwon),장일두(Il-Doo Chang) 한국통신학회 2013 한국통신학회 학술대회논문집 Vol.2013 No.1

인간 체외수정 및 배아이식에 있어서 과배란 유도 과정에 사용한 GnRH Agonist가 배란 전 난포내 과립 세포의 세포자연사에 미치는 영향

양현원,권혁찬,황경주,박종민,오기석,윤용달,Yang, Hyun-Won,Kwon, Hyuck-Chan,Hwang, Kyung-Joo,Park, Jong-Min,Oh, Kie-Suk,Yoon, Yong-Dal 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1

There have been many reports to date regarding the role of GnRH as a local regulatory factor of ovarian function as studies of human and rat ovaries revealed GnRH and its receptor. In recent studies it has been shown that GnRH directly causes apoptosis in the granulosa cells of the rat ovary, and such results leads to the suggestion that the use of GnRH agonist for more stable long term ovarian hyperstimulation in human IVF-ET programs causes granulosa cell apoptosis which may lead to follicular atresia. Therefore this study attempts to determine if granulosa-luteal cell apoptosis occurs in patients during IVF-ET programs in which GnRH agonist is employed for ovarian hyperstimulation. The quality of oocyte-cumulus complexes obtained during ovum pickup procedures were assessed morphologically and then the fertilization rate and developmental rate was determined. Apoptotic cells among the granulosa-luteal cells obtained during the same procedure were observed after staining with Hematoxylin-eosin. The fragmentation degree of DNA extracted from granulosa-luteal cells was determined and comparatively analyzed. There was no difference in the average age of the patients, the number of oocytes retrieved, and fertilization and developmental rates between the FSH/hMG group and GnRH-long group. There was also no difference in the apoptosis rate and pyknosis rate in the granulosa-luteal cells between the two groups. However, when the oocyte-cumulus complexes were morphoogically divided into the healthy group and atretic group without regard for the method of hyperstimulation, the results showed that the number of oocytes obtained averaged $11.09{\pm}8.75\;and\;10.33{\pm}4.53$ per cycle, respectively, showing no significant difference, but the fertilization rate (77.05%, 56.99%, respectively, p<0.01) and developmental rate (65.96%, 41.51%, respectively, p<0.01) was significantly increased in the healthy group when compared to the atretic group. The degree of apoptosis in the granulosa-luteal cells showed that in the healthy group it was 2.25% which was not significantly different from the atretic group (2.77%), but the pyknosis rate in the atretic group (27.81%) was significantly higher compared to the healthy group (11.35%, p<0.01). The quantity of DNA fragmentation in the FSH/hMG group was 32.22%, while in the GnRH-long group it was 34.27%, showing no significant difference. On the other hand the degree of DNA fragmentation was 39.05% and 11.83% in the healthy group and atretic group, respectively, showing significantly higher increase in the atretic group (p<0.01). The above results suggest that death of granulosa-luteal cells according to the state of the oocyte-cumulus complex is more related to pyknosis rather than apoptosis. Also, the GnRH agonist used in ovarian hyperstimulation does not seem to directly affect the apoptosis of retrieved oocytes and granulosa-luteal cells, and which is thought to be due to the suppression of the apoptogenic effect of GnRH agonist as a result of the high doses of FSH administered.

난소의 자궁내막종에 대한 다양한 치료적 적용에 따른 체외수정 및 배아이식술 결과의 비교 연구

이방현,권혁찬,이재현,김보현,이상희,박민혜,이병관,임정애,Lee, Bang-Hyun,Kwon, Hyuck-Chan,Lee, Jae-Hyun,Kim, Bo-Hyun,Lee, Sang-Hee,Park, Min-Hye,Lee, Byung-Kwan,Lim, Jung-Ae 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.2

Objective: To compare COH characteristics and IVF outcomes among IVF-ET patients who were treated with various therapeutic modalities for ovarian endometriomas and to propose effective pre-cyclic therapeutic modalities to improve IVF-ET outcomes in the patients with ovarian endometriomas. Methods: All cases that had undergone IVF-ET after laparoscopy between January 1997 to August 2003 were reviewed. Forty-eight patients with tubal factor were assigned to Group I. Twenty seven, 22 and 38 patients diagnosed as severe pelvic adhesion with ovarian endometriomas by laparoscopy received only medical therapy (Group II), cyst aspiration (Group III), and sclerotherapy (Group IV), respectively. Laparoscopic cystectomy was performed in 20 patients (Group V). Resistance index was measured on day administering hCG. Results: As compared with Group I, in Group II resistance index increased (p<0.05) but number of oocytes, good-quality oocyte ratio (mature and intermediate oocytes/total retrieval oocytes), fertilization rate, and embryo development rate decreased (p<0.05). In Group III fertilization rate and embryo development rate decreased (p<0.05). There was no difference between Group IV and Group I in all parameters except basal FSH which increased (p<0.05). In Group V basal FSH, and resistance increased (p<0.05) and number of oocytes and good-quality oocytes ratio decreased (p<0.05). Conclusion: Sclerotherapy is an effective therapeutic option which can be done prior to IVF-ET cycles in the patients with ovarian endometriomas. Further studies on a large scale are necessary to confirm these data.

인간의 착상 기전을 연구하기 위한 3차원적 자궁내막 모델 확립

박동욱,양현원,권혁찬,장기홍,김세광,조동제,오기석,Park, Dong-Wook,Yang, Hyun-Won,Kwon, Hyuck-Chan,Chang, Ki-Hong,Kim, Sei-Kwang,Cho, Dong-Jae,Oh, Kie-Suk 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1

In order to study the implantation mechanism various methods for culture of endometrial cells in vitro have been attempted. However, a disadvantage is that primary cultures of stromal and epithelial cells do not have the ability to differentiate, and therefore cannot be reproduced in the same manner as in vivo endometrium. The object of this study is to establish a three dimensional culture of endometrial cells which are both morphologically and functionally identical to in vivo endometrium. Endometrial tissues obtained after hysterectomies were cut into thin slices and treated with collagenase and trypsin-EDTA. The stromal cells and the epithelial cells were separated by centrifugation and cultured for 24 hours in DMEM media containing 10% FCS, 100 nM progesterone, and 1 nM estradiol. The cultured stromal cells were mixed with collagen gel and solidified, after which it was covered with matrigel. Epithelial cells were inoculated on the top and then cultured for 3 days. The three dimensionally cultured endometrial cells were stained for integrin ${\alpha}1,\;{\alpha}4,\;{\beta}3$, and cyclooxygenase-l, -2 by immunohistochemistry, which all showed strong expression. The cultured epithelial cells showed the formation of microvilli, tight junctions and pinopodes by electron microscopy. Studies are currently under way utilizing this three dimensional culture model to ascertain the interaction between the embryo and human endometrial cells at the time of implantation, and it is thought that further studies into a new culture environment which would allow longer periods of culture will be necessary.

불임여성의 식생활 특성

오지수,정혜자,권혁찬,박원일,궁미경,김화영,Oh, Ji-Soo,Jung, Hye-Ja,Kwon, Hyuck-Chan,Park, Won-Il,Koong, Mi-Kyoung,Kim, Wha-Young 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.4

Objective: The purpose of this study was to identify dietary factors related to infertility in Korean women through a case-control study. Methods: The case group was composed of 236 women who had been diagnosed as infertility in hospital. The control group of 181 healthy women with children were recruited from local immunization centers. Socio-economic status, medical history, dietary intakes using food frequency questionnaire and stress were surveyed by interview. Anthropometric measurements were made and the causes of infertility were identified through medical records. Fasting blood samples were taken from subgroup of the subjects. Results: The mean age of infertile and control groups was 31.1 and 32.4 years, respectively and the difference was statistically significant. The mean Body Mass Index of infertile women was not significantly different from control women, however, Waist/ Hip Ratio and Triceps Skinfolds Thickness were significantly lower in infertile women than in control women. The dietary intake status was generally satisfactory in both groups. The intakes of energy, protein, fat, carbohydrate, retinol, vitamin B2 and niacin were lower in infertile women than in control women. The infertile women also showed lower intakes of animal foods. No differences were found between two groups in serum concentrations of albumin, hemoglobin, Fe, TIBC, total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, C3, IgA, IL-2, however, infertile women showed higher levels of Zn and IgG. The stress score was higher in infertile women. Conclusions: From the results of this study, dietary factors and nutritional status do not seem to be directly related to infertility. However, the intertile women have lower nutrient intake and lower body fat content than control women. Further researches are needed according to the causes of infertility for long term to establish the relationship between dietary factors and infertility.

체외수정시술시 미세조작술에 의한 수정률 향상과 임신에 관한 연구

이호준,최규완,전종영,박종민,권혁찬,김문규,Lee, Ho-Joon,Choi, Kyoo-Wan,Jun, Jong-Yung,Park, Jong-Min,Kwon, Hyuck-Chan,Kim, Moon-Kyoo 대한생식의학회 1990 Clinical and Experimental Reproductive Medicine Vol.17 No.1

The purpose of this study is to improve fertilization rate in IVF-ET program of patients with male infertility used micromanipulation technique, partial zona dissection (PZD) or micro-insemination by sperm transfer (MIST). The results were as follows 1. The fertilization rate of non-micromanipulated oocytes and micromanipulated (PZD) oocytes were 12.5% (n=2) and 42.2% (n=19), respectively, and showed significant differences between two groups (p<0.05). 2. The fertilization rate of micromanipulated (MIST) oocytes was 30% (n=27). 3. The damage rate of Group 1 (PZD) and Group 2 (MIST) were 15.7% (3/19) and 29.6% (8/27), respectively. 4. One pregnancy resulted following replacement of micromanipulated (MIST) embryos in 4 patients.

반응성 산소족이 사람 정자의 운동성에 미치는 영향

강희규,김동훈,한성원,김묘경,권혁찬,이호준,김문규,Kang, Hee-Gyoo,Kim, Dong-Hoon,Han, Sung-Won,Kim, Myo-Kyung,Kwon, Hyuck-Chan,Lee, Ho-Joon,Kim, Moon-Kyoo 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.4

Objective: To investigate the effects of ROS on kinematic parameters in human spermatozoa. To verify the changes in above parameters, human spermatozoa were incubated with xanthine-xanthine oxidase (X-XO), $H_2O_2$, sodium nitroprusside (SNP) or lymphocyte. Otherwise, spemlatozoa were incubated under low $O_2 (5%) condition. Methods: CASA was employed to analyze sperm motion parameters. Results: Under $H_2O_2 treatment, all kinematic parameters of spermatozoa were dramatically increased during 30 min, but gradually decreased thereafter. Under the low concentration of $H_2O_2 (125 ${\mu}M$ and 250 ${\mu}M$), the movement velocity (VAP, VCL, VSL) decreased, but forward movement increased. Under the 1mM $H_2O_2, sperm showed reduced kinematic parameters except during first 30 min of incubation. In the cases of X-XO and SNP treatment, the movement velocity increased but the forward movement reduced. After incubation for 3 hr treatment, the kinematic parameters gradually decreased in high concentration of X-XO. However these parameters maintained or increased in low concentration of X-XO. There was no obvious changes in the above parameters in the high concentration of SNP. In the presence of high concentration of lymphocytes, all parameters decreased. Under the 5% $O_2 condition, the parameters of the movement velocity and movement pattern increased, but forward movement decreased. Taken togethers, it suggested that ROS increased the movement velocity but decreased the forward movement and lateral head replacement. $H_2O_2, X-XO, SNP and lymphocyte treatment significantly increased capacitated spermatozoa within I h of incubation. There was no significant difference in capacitation between low- and high $O_2 group. Conclusion: The early onset of capacitation in the presence of ROS suggest that ROS might be a positive regulator of sperm capacitation and hyperactivation. These results demonstrate that low concentration ROS facilitates the movement velocity but high concentration ROS was inhibitory.

인간 난소의 과립 세포 배양 중 Leptin이 스테로이드 생성에 미치는 영향

김세광,김명신,황경주,권혁찬,조동제,Kim, Sei-Kwang,Kim, Myong-Shin,Hwang, Kyung-Joo,Kwon, Hyuck-Chan,Cho, Dong-Jae 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

Objective: To elucidate the location of leptin and receptors of ovary specimens obtained from patients undergoing hysterectomy by immunohistochemical staining and to determine the effect of leptin on the steroidogenesis of cultured granulosa cells. Method: In the culturing process of the granulosa cells, FSH (1 IU/ml)and leptin (50 ng/ml), IGF-I (50 ng/ml) was administered to each study group (Group I: FSH; Group II: FSH, leptin; Group III: FSH, IGF-I; Group IV: FSH, IGF-I, leptin), and the levels of estradiol, progesterone, androstenedione in the culture media was measured by radioimmunoassay. Statistical analysis was conducted by one-way ANOVA with Scheffe test. Results: The results showed that leptin and leptin receptors were both found to be strongly stained in granulosa and theca cells, and also in some interstitial cells. Leptin receptors were also observed in cultured granulosa cells. While there was no statistically significant difference in the androstnedione concentrations between the groups, estradiol concentrations was significantly decreased in Group IV ($2202.0{\pm}151.14$ pg/ml) compared to Group III ($2859.0{\pm}122.6$ pg/ml), and progesterone concentrations were also significantly decreased in Group II($4696.3{\pm}190.6$ ng/ml) and Group IV ($4517{\pm}206.78$ ng/ml) compared to Group III($5546.0{\pm}179.5$ ng/ml). Conclustion: The study result of this study suggest that leptin is directly involved in the regulation of ovarian functions, in particular steroidogenesis.

착상관련 질환 환자의 자궁내막에 있어서 프로게스테론 수용체, integrinβ₃, cyclooxygenase-2(COX-2)의 발현 양상

이준서(June Seo Lee),박동욱(Dong Wook Park),권혁찬(Hyuck Chan Kwon),김미란(Mi Ran Kim),황경주(Kyoung Joo Hwang),주희재(Hee Jae Joo),조동재(Dong Jae Cho),김세광(Sei Kwang Kim),오기석(Kie Suk Oh) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6

연구목적 : 착상관련 질환을 가지고 있는 한국 여성의 자궁내막 조직과 정상적인 여성의 내막 조직을 비교하여 병인의 특성과 진단에 도움을 주는 면역 화학적 지표를 발견하고자 하였다. 연구방법 : 정상 소견을 가진 20명(1군),자궁내막 유착증 5명(2군),자궁내막증 20명(3군)을 대상으로 배란일로부터 7-8일째 성선호르몬을 측정하였고 자궁내막세포를 채취하여 PR, integrin β3, COX-2의 발현을 면역조직화학적 방법으로 염색하였다. 결과 : 성호르몬 수용체는 PR의 발현이 2군에서는 1군에 비하여 감소하였으나 3군에서는 증가되는 양상을 보인다. Integrinβ3의 발현은 2군과 3군 모두에서 정상 대조군에 비하여 유의하게 감소하였다. COX-2의 발현은 2군에서는 뚜렷하게 감소하였으나 3군에서는 선상피세포에서의 발현이 다소 증가되고, 기질세포에서는 뚜렷이 증가되었다. 결론 : PR, Integrin 3, COX-2는 자궁내막증 환자와 자궁내막 유착증 환자에 있어서 대조군에 비하여 서로 다른 발현을 보임으로써 착상관련질환을 가진 환자들의 진단 및 치료에 유용한 지표가 될 수 있다고 생각된다. Objective : We compared the expression pattern of progesterone receptor, integrin 3, cyclooxygenase-2 (COX-2) in in-phased endomerium of patient with the disease related implantation and control group, and tried to confirm the clinical efficacy of the immunohistochemical markers for discrimination of occult uterine receptivity defect in in-phase endometrium.Study design : Endometrial tissues were obtained from 60 women with normal (group 1; n = 20), uterine synechiae (group 2; n = 15), and endometriosis (group 3; n = 25). On 7 ∼ 8 days after ovulation (POD 7 ∼ 8), sex hormone levels were measured and immunohistochemical staining of PR, integrin 3, and COX-2 expression were performed. Results : PR was decreased in the group 2 and increased in the group 3 comparing with the group 1. integrin 3 expression was significantly decreased in the group 2 and 3. COX-2 expression was significantly decreased in the group 2. But, in the group 3, COX-2 expression was slightly increased in glandular epithelial cells, and significantly increased in stromal cells. Conclusions : In-phase biopsies from patients with endometriosis and uterine synechiae showed different expression pattern of integrin 3, COX-2, and PR compared to the control. The aberrant expression of immunohistochemical markers be associated with occult uterine receptivity defect and produce the useful diagnostic method.

다낭성 난소 증후군 환자에서 체중과 임신이 내분비 양상 및 당 대사에 미치는 영향

황경주(Kyung Joo Hwang),오기석(Kie Suk Oh),김세광(Sei Kwang Kim),권혁찬(Hyuck Chan Kwon),박영한(Young Han Park),양정인(Jung In Yang),유희석(Hee Suk Rhyu) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.3

N/A Objective: The purpose of this study attempts to determine the endocrinologic characteristics and changes in glucose metabolism before/during pregnancy according to different body weights in women with Polycystic ovarian syndrome (PCOS). Methods: 94 women dia with PCOS were evaluated through measuring serum hormone levels and oral glucose tolerance tests preconceptionally and gestationally. Results: In patients who were of normal weight showed significantly increased serum LH levels compared to those who were overweight (12.8+-0.9 Vs. 7.1+-0.8 mIU/ml, p= 0.000), and the serum levels of insulin was increased significantly in the overweight group (7.1+-0.7 Vs. 15.2+-2.8 ulU/ml, p= 0.000). the IGFBP-I (32.8+-10.6 Vs. 8.3+-2.5 ng/ml, p=0.034) and SHBG (55.8+-4.2 Vs. 37.1+-3.1 nmol/ml, p= 0.001) were significantly lower in the ovnweight group. The oral glucose tolerance test before/after pregnancy showed increased frequency of abnormal glucose metabolism, in both of the non-obese group (38.8%, 26,9%) and the obese group (64.2%, 53.9%) compared with normal population. Conclusion: It is thought that in the normal weight group with polycystic ovarian syndrome androgen production is stimulated in the theca cells by abnormally high LH secretion, while in the overweight group the hyperinsulinemia state which decreases the SHBG and IGFBP-I, lead to increase biologically active hee androgens and IGF-I and increase insulin binding to its receptor. And during/before pregnancy, women with PCOS showed that incidence of abnormal glucose metabolism was significantly increased in both of non-obese and obese groups.