http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Multilayered Cell Sheets of Cardiac Reprogrammed Cells for the Evaluation of Drug Cytotoxicity
권성필,Song Seuk Young,유진,Kim Han Young,이주로,강미경,Sohn Hee Su,Go Seokhyoung,정문교,Hong Jihye,임송현,Kim Cheesue,문상준,차국헌,김병수 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5
Background: Various cell-culture systems have been used to evaluate drug toxicity in vitro. However, factors that affect cytotoxicity outcomes in drug toxicity evaluation systems remain elusive. In this study, we used multilayered sheets of cardiac-mimetic cells, which were reprogrammed from human fibroblasts, to investigate the effects of the layer number on drug cytotoxicity outcomes. Methods: Cell sheets of cardiac-mimetic cells were fabricated by reprogramming of human fibroblasts into cardiac-mimetic cells via coculture with cardiac cells and electric stimulation, as previously described. Double-layered cell sheets were prepared by stacking the cell sheets. The mono- and double-layered cell sheets were treated with 5-fluorouracil (5-FU), an anticancer drug, in vitro. Subsequently, apoptosis and lipid peroxidation were analyzed. Furthermore, effects of cardiac-mimetic cell density on cytotoxicity outcomes were evaluated by culturing cells in monolayer at various cell densities. Results: The double-layered cell sheets exhibited lower cytotoxicity in terms of apoptosis and lipid peroxidation than the mono-layered sheets at the same 5-FU dose. In addition, the double-layered cell sheets showed better preservation of mitochondrial function and plasma membrane integrity than the monolayer sheets. The lower cytotoxicity outcomes in the double-layered cell sheets may be due to the higher intercellular interactions, as the cytotoxicity of 5-FU decreased with cell density in monolayer cultures of cardiac-mimetic cells. Conclusion: The layer number of cardiac-mimetic cell sheets affects drug cytotoxicity outcomes in drug toxicity tests. The in vitro cellular configuration that more closely mimics the in vivo configuration in the evaluation systems seems to exhibit lower cytotoxicity in response to drug.
권성필(Kwon, Seong-Pil),김형권(Kim, Hyeong-Gweon),윤헌주(Yoon, Heon-Joo),리용운(Ra, Yong-Woon),손윤석(Sohn, Yoon-Suk),신동일(Shin, Dong-Il) 한국화재소방학회 2009 한국화재소방학회 학술대회 논문집 Vol.2009 No.춘계
본 연구에서는 친환경 인테리어용으로 개발된 벽포지에 대해 방염성능이 시험되었다. 실제로 방염성능은 "45<TEX>$^{\circ}$</TEX> 연소시험" 및 "접염시험", 그리고 "연기밀도시험"에 의해서 수행되었다. 벽포지의 주성분이 고온에서 쉽게 용융하는 PET(폴리에스터) 이다. PET의 이런 물성으로 인하여 "45<TEX>$^{\circ}$</TEX> 연소시험"과 "연기밀도시험"의 요구조건들은 쉽게 만족시킬 수 있었지만, 용융 물품에 대해 추가로 요구되는 "접염시험"은 통과하기 어려웠다. 총 47종 109점에 대해 방염성능시험을 수행한 결과 18종 54점이 요구조건을 만족하였다. 일반적으로 보드에 배접된 상태로 시공되는 모듈의 난연성능을 개선하기 위해서는 벽포지의 방염성능뿐만 아니라 보드, 접착제 등의 특성에 관한 보다 많은 연구가 필요하다.
권성필(Kwon, Seong-Pil),윤헌주(Yoon, Hun-Ju),김형권(Kim, Hyeong-Gweon),라용운(Ra, Yong-Woon),사공성호(SaKong, Seong-Ho),신동일(Shin, Dong-Il) 한국화재소방학회 2008 한국화재소방학회 학술대회 논문집 Vol.2008 No.추계
In this work wood crib burning behaviors have been simulated by using the FDS(Fire Dynamic Simulator) program. Wood cribs are regularly stacked arrays of wood sticks, and available for the performance rating of fire-extinguishers. On the basis of an angle iron supporter 26 layers of wood sticks have been stacked up. Each layer consists of 5 or 6 wood sticks which are placed in parallel, with a constant distance, and in alternating rows. They are laid between the horizontally adjacent sticks at the before last layer. The wood crib is ignited instantaneously by an amount of burning gasoline below. A comprehensive simulation of such a practical sophisticated combustion is still too difficult to realize with any currently available computer, although the performance of modern processors is getting better everyday. We could carry it out here through parallel computing on the HPC(High Performance Computing) cluster as the feasible alternative. At last the validation has been executed by means of temperature distribution data measured by the thermal video camera.