국내 6개 하천에서 어도를 이용하는 어류상

김동억,박제철,황종서,김동섭,이완옥,황길순 한국하천호수학회 2020 생태와 환경 Vol.53 No.3

The use of fishway has been investigated up to 2~9 years, continuously each month and over the long term that installed in 6 domestic rivers. The fish’s use of fishway has been investigated as direct collection by trap in exit section of the fishway. The population structure of fish using 6 fishway and the fish that live in the river showed difference. About 2 to 7 species of fish took possession of more than 80% of total use of fish in fishways. This seems to be not the results of attraction flow in fishway but specialized result of physiology characteristic followed by species of fish. In addition, the distribution of fish species using fishway was different with the fishways installed in river. This difference seems to be consequence of the place that of the river and fishway types. Generally, the fish species were less and had high ratio of home migratory fish in vertical slot type fishway, which shows relatively fast velocity of water flow. This analysis result of major fish species using fishway can be used in planning fishway and to choose major targeted species for the planning.

뇌졸중 최신지견 2009: 뇌졸중 기초/중개 연구

김동억 대한뇌졸중학회 2010 Journal of stroke Vol.12 No.1

Translational research helps translate scientific discoveries from bench to clinic. In this brief review, the results of recent translational stroke research are summarized. It covers following topics: 1) peroxisome proliferator-activated receptor-γ (PPAR-γ) to protect against ischemic cerebral infarction, 2) N-methyl-d-aspartate glutamate (NMDA) receptor-dependent activation of sterol regulatory element binding protein-1 (SREBP-1) in ischemic neuronal injuries, 3) regulatory T cells as cerebroprotective immunomodulators in stroke, 4) cerebral interleukin-17-producing γδT cells in the delayed phase of ischemic brain injury, 5) reduction of endoplasmic reticulum (ER) stress and alleviation of atherosclerosis, 6) platelet dense-granule secretion to play a critical roles in thrombosis and subsequent vascular remodeling, and 7) pericyte contraction induced by oxidative-nitrative stress and noreflow phenomenon in cerebral infarction.

The Life of Professor C. Miller Fisher: A Giant of Stroke Medicine

김동억 대한뇌졸중학회 2020 Journal of stroke Vol.22 No.3

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C17.2 신경줄기세포 및 일차 신경줄기세포의 허혈 내성

김동억 대한뇌졸중학회 2006 Journal of stroke Vol.8 No.1

Background: It has not been studied whether neural stem cells (NCSs) have ischemic tolerance phenomenon, which could protect the cells when exposed to non-permissive environments. Methods: C17.2 neural precursor cells (NPCs) and freshly-isolated NPCs growing as neurospheres were used to study: (a) if NPCs survival rate could be decreased by exposing them to in vitro ischemia, and (b) if the survival rate could be improved by a preceding hypoxia of short duration. After a baseline experiment to verify that the cells have characteristics of NSCs, 30 min or 1 hour of ischemia was applied to the cells of the preconditioning-only group and ischemia-after-preconditioning group. Forty eight hours later, 12 or 24 hours of main ischemia was given to the cells of the ischemia-after-preconditioning group and ischemia-without-preconditioning group. Control group cells were incubated under normoxic conditions for equivalent durations. After 2 or 12 hours of reperfusion period, luciferase viability assays, lactate dehydrogenase release cytotoxicity assays, and annexin/propidium-iodide flow cytometry assays of apoptosis/necrosis were performed. Results: The C17.2-Luc NPCs and primary NPCs had well-established characteristics of neural stem cells. Intact NPCs were decreased in numbers after 12 or 24 hours of in vitro ischemia followed by 2 or 12 hours of reperfusion. The cell death rate could be reduced (20%~60%) by a preceding hypoxia of 30 min or 1 hour duration. The flowcytometry study suggested that the decreased rates of aopototic or apopto-necrotic deaths were responsible for the improved cell viability. Conclusions: In summary, this study demonstrated that the hypoxic preconditioning could protect NSCs against subsequent lethal ischemia.

동맥경화 병태생리 및 치료 효과를 탐지하는 Cathepsin-B 단백분해효소 활성도 광학 분자영상

김동억,김정연,정상욱,김어진 대한신경과학회 2009 대한신경과학회지 Vol.27 No.1

Background: Protease-sensing molecular optical imaging can potentially identify vulnerable atherosclerotic plaques before cerebral infarcts occur. However, several translational issues need to be resolved before implementing human trials and clinical applications of the technique. We investigated whether molecular optical imaging reflects (1) atherosclerosis pathophysiology and (2) the atherosclerosis-modulating effect of a Western diet or atorvastatin treatment in ApoE-knockout mice. Methods: We fed 21 8-week-old ApoE-knockout mice a normal chow diet (n=7), a Western diet (n=7), or a Western diet with atorvastatin (n=7) for 14 weeks. Blood was collected for measuring cholesterol levels at 24 hours after injecting the cathepsin-B (CatB) probe into the tail vein. Near-infrared fluorescence reflectance imaging of CatB activity was performed after excising the aorta. Results: CatB molecular optical imaging precisely reflected the pathophysiologic alteration of atherosclerosis. The CatB activity signal was significantly stronger in the Western diet group (49.3±26.0 [mean±SD], arbitrary units) than in the atorvastatin group (25.3±8.7) and the normal chow diet group (20.6±21.1). The effect of the statin on CatB activity was independent of cholesterol lowering. Conclusions: CatB-sensing molecular optical imaging represents a valuable tool for evaluating atherosclerosis pathophysiology and for monitoring the effects of antiatherosclerotic therapeutic interventions. Background: Protease-sensing molecular optical imaging can potentially identify vulnerable atherosclerotic plaques before cerebral infarcts occur. However, several translational issues need to be resolved before implementing human trials and clinical applications of the technique. We investigated whether molecular optical imaging reflects (1) atherosclerosis pathophysiology and (2) the atherosclerosis-modulating effect of a Western diet or atorvastatin treatment in ApoE-knockout mice. Methods: We fed 21 8-week-old ApoE-knockout mice a normal chow diet (n=7), a Western diet (n=7), or a Western diet with atorvastatin (n=7) for 14 weeks. Blood was collected for measuring cholesterol levels at 24 hours after injecting the cathepsin-B (CatB) probe into the tail vein. Near-infrared fluorescence reflectance imaging of CatB activity was performed after excising the aorta. Results: CatB molecular optical imaging precisely reflected the pathophysiologic alteration of atherosclerosis. The CatB activity signal was significantly stronger in the Western diet group (49.3±26.0 [mean±SD], arbitrary units) than in the atorvastatin group (25.3±8.7) and the normal chow diet group (20.6±21.1). The effect of the statin on CatB activity was independent of cholesterol lowering. Conclusions: CatB-sensing molecular optical imaging represents a valuable tool for evaluating atherosclerosis pathophysiology and for monitoring the effects of antiatherosclerotic therapeutic interventions.

토양 수분함량이 토마토 묘의 생육 및 항산화 효소에 미치는 영향

김동억,강정균,신윤아,홍순중,이운용,우영회,Kim, D.E.,Kang, J.K.,Shin, Y.A.,Hong, S.J.,Lee, W.Y.,Woo, Y.H. 국립한국농수산대학교 교육개발센터 2019 현장농업연구지 = Journal of practical agricultural resear Vol.21 No.2

본 연구는 토마토 육묘 시 생육과 묘소질에 영향을 줄 수 있는 요인 중에서 수분함량이 모종의 생육과 항산화효소 형성에 미치는 영향, 엽온의 변화에 대한 관찰을 수행하였다. 실험 방법은 토마토 모종을 관수한 상태의 무게가 50g, 40g, 30g가 되도록 관수를 하였고 각 묘 및 트레이의 무게를 시간마다 측정하였으며, 열화상 카메라를 이용하여 엽온을 측정하였고, 실험 후 각 묘의 항산화효소를 측정하여 스트레스 여부를 확인하였다. 실험 결과, 상토 무게의 변화는 주간 감소량 보다 야간의 감소량이 더 적은 것으로 나타났으며 각 묘의 무게별 감소량은 차이가 없는 것으로 나타났다. 또한 상토 무게에 따른 생육 변화도 차이가 없는 것으로 나타났다. 하지만 플라보노이드 및 항산화 효소는 상토 무게 50g에서 가장 높게 나타났다. 이는 근권부에 과습 상태가 장시간 지속되어 스트레스를 받은 것으로 판단되며, 추후 장시간 생육 변화, 실시간 환경 변화 조사 및 상토 종류에 따른 증발산량 조사가 필요할 것으로 사료된다.

IPAK회관에 소프트웨어 부품 도서관을 만들자

김동억,Kim, Dong-Eok 한국IT전문가협회 1997 소프트웨어세계 Vol.3 No.-

신경과학 분야에서의 광학적 분자영상

김동억 대한신경과학회 2006 대한신경과학회지 Vol.24 No.2

Molecular imaging is leading an important role in the era of molecular medicine. Optical imaging, a rising star in the filed of molecular imaging, largely consists of bioluminescent imaging and fluorescent imaging. It has been shown that well-aimed and creatively-built optical-imaging-reporters let researchers explore and answer a lot of biologically important questions in living subjects. Despite relatively short history, molecular optical imaging is rapidly being implemented not only in many clinical areas but also in various research fields from tracking gene expression, protein-protein interaction or migrating cells to molecular diagnosis and treatment.

Fault Analysis Method for Power Distribution Grid with PCS-based Distributed Energy Resources

김동억,조남훈 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.2

In this study, we propose a fault analysis method for a power distribution grid with PCSbased DERs. We first explain the characteristics of a PCS-based DER. According to the characteristics, the DER is considered as a current-controlled voltage source, which produces varying voltages within a certain limit so that currents equal to given references flowing from the DER to the grid (currents controlled). So, we introduce the symmetrical equivalent models in the form of varying voltage source for fault analysis and then, construct a convex optimization problem to solve the fault problem associated with the equivalent models and grid conditions. Thus, the proposed method enables to perform a proper fault analysis considering the characteristics of the DER, which are currents controlled, voltage limited, and unity power factor achievement. To verify the validity of the proposed method, we perform computer simulations with the proposed method and with MATLAB Simulink, and the results are compared.

뇌졸중 실험연구: 2007년 최신지견

김동억 대한뇌졸중학회 2008 Journal of stroke Vol.10 No.2