월동저온, 저온기간 및 가지직경이 참다래 궤양병의 진전과 조직내 병원균 이행에 미치는 영향

고숙주,강범룡,이용환,김용환,김기청 한국식물병리학회 2000 식물병연구 Vol.6 No.2

전남지역의 수박에 발생하는 바이러스 병 발생 실태

고숙주,이수헌,박진우,이용환,차광홍,양광열 한국식물병리학회 2004 식물병연구 Vol.10 No.1

Recent occurrence of virus diseases on watermelon plants cultivated in Jeonnam province was investigated from 1998 to 2002. While virus diseases were severely occurred on watermelon cultivated in green house in 1998, those of open field were severer than in green house since 2000. When 128 samples collected from different fields were examined by electron microscopy, 87.8% of the samples contained rod-shaped or filamentous virus particles. RT-PCR analysis of the samples revealed that Cucumber green mottle mosaic virus (CGMMV) was only detected from collected samples at May. Watermelon mosaic virus (WMV) was most frequently found and CGMMV and Zucchini yellow mosaic virus (ZYMV) were slightly at June and July. However Cucumber mosaic virus (CMV) and Papaya ringspot virus (PRSV) have not been detected.

CGMMV 감염시기가 수박과 오이의 생육 및 품질에 미치는 영향

고숙주,박진우,최홍수,이용환,이태선,양광열 한국식물병리학회 2004 식물병연구 Vol.10 No.1

We investigated the effect of infection time of CGMMV on the growth and quality of watermelon and cucumber plants. The effect (damages by CGMMV) was estimated on the watermelon where CGMMV had been inoculated at different growth stages, vegetative (transplanting stage, vegetative growth stage) and reproductive growth stage (fruiting stage and fruit hypertrophy stage). In the case of cucumber, CGMMV was inoculated at transplanting stage and first flowering stage, respectively. When watermelon was infected with CGMMV at vegetative growth stage, vine length, internode length, leaf area, and fruit weight of the plants largely decreased compared with control plants, while the infected plant growth was not very different from control plants when it was infected at reproductive growth stage. Brix of the fruit of watermelon also decreased when the plants was infected with the virus earlier than fruiting stage. The occurrence of ‘Pisubag’, internal discoloration and decomposition of watermelon fruits, tended to be increased as earlier infection time with CGMMV. In the case of cucumber, infection time with CGMMV did not influence earlier growth of the plants, but did later growth showing that plant height, vine length, internode length, number of leaf, leaf wide, and leaf length of the plants decreased as infection time became to be earlier.

오이 종자로부터 CGMMV의 검출과 품종 저항성 검정

고숙주,박진우,이용환,촤광홍,최형국 한국식물병리학회 2004 식물병연구 Vol.10 No.2

To analyze the relationship between CGMMV occurrence and seed contamination by cucumber cultivars, cucumber cultivars of CGMMV infected field and diseased degree were investigated in cucumber main cultivation regions of Jeonnam province from 1999 to 2002. While the diseased degree was different by years and cultivars, CGMMV occurred for 4 years in ‘Janghyeongnakhap’, for 3 years in ‘Jangjukcheongjang’, and for 2 years in ‘Gyeoulsali’ and ‘Gyeoulnagi’. CGMMV was detected in seeds of ‘Janghyeongnakhap’ and ‘Jangjukcheongjang’ by ELISA test, and seed of ‘Jangjukcheongjang’ showed positive reaction in bioassay. As a result of resistant test of 34 cultivars to CGMMV, all cultivars showed mosaic symptoms in pot test but only ‘Hanboksamcheok’ showed mild mosaic symptom in field test.

수박 CGMMV의 즙액전염 양상과 전염 억제제의 효과

고숙주,이용환,촤광홍,이태선,박인진 한국식물병리학회 2004 식물병연구 Vol.10 No.2

This study was conducted to investigate the sap transmission pattern of CGMMV and to select the disinfectant of sap transmission on watermelon cultivation. Being investigated CGMMV occurrence change after transplanting two diseased plants among 47 plants on the watermelon house, disease spread rapidly from early growth stage of 4% to late growth stage of 83%. When the grafting knife and topping scissor was dipped onto the several disinfectants before grafting or topping, disease suppressed 87-95% on whole milk, skim milk, tri-sodium phosphate, and sodium hypo-chloride solution. Disinfection effects of 88.2% and 91.2% on whole milk and skim milk were showed when the hand dipped on the disinfectants.

전남 지역의 토마토반점위조바이러스병 발생 양상

고숙주,강범용,최덕수,김도익,이관석,김창석,최홍수 한국식물병리학회 2013 식물병연구 Vol.19 No.4

Tomato spotted wilt virus (TSWV) was occurred at 8 areas including Naju, Suncheon, Younggwang, Youngam,and Shinan in Jeonnam province and the crops of Younggwang were severely damaged by TSWV. The hotpepper (Capsicum annuum), bell pepper (Capsicum annuum v ar. angulosum) and tomato (Solanumlycopersicon) in greenhouse and hot pepper in open field were infected by TSWV. Especially, hot pepper wasseverely damaged by TSWV infection. The survey data indicated that 1.1−30% in the nursery field at Naju,Suncheon, and Jangheung were infected by TSWV. Plants were infected by TSWV from early June toAugust. However, TSWV-infected seedlings from nursery fields showed the disease symptoms from May aftertransplanting. In pepper greenhouses, Frankliniella occidentalis was more dominant insect vector thanFrankliniella intonsa. But in open field, the population of insect vector was opposed to greenhouse. Inaddition, the removal of weeds was able to delay the incidence of TSWV via side-window of greenhouse inWinter. Taken together, the control of weed and insect vector nearby side-window of greenhouse is importantto prevent TSWV infection of plants. 전남 지역에서 토마토반점위조바이러스병은 나주, 순천,영광, 신안 등 8개 시군에서 발생되었으며, 영광 지역이가장 심하였다. 시설재배에서는 주로 고추, 피망, 토마토에 발생하였고, 노지재배에서는 고추의 피해가 컸다. 고추 육묘장에서 Tomato spotted wilt virus(TSWV) 발생을조사한 결과, 나주, 순천, 장흥 지역에서 발병주율은 1.1−30%로 조사되었다. TSWV은 일반적으로 6월 상순에 초발하여 8월까지 지속적으로 발병이 증가하였으나, TSWV에 감염된 묘가 정식된 경우 정식 초기인 5월 상순부터발생을 시작하였다. 시설재배지에서 꽃노랑총채벌레가 대만총채벌레보다 황색점착트랩에 높은 밀도로 채집되었고,노지고추에서는 대만총채벌레가 꽃노랑총채벌레보다 우점하였다. 동절기 휴경기에 하우스 내와 측창 사이의 잡초를 완전히 제거한 경우에는 방치한 포장에 비해 바이러스 초발일이 1개월 정도 지연되는 경향을 보였고, 측창쪽에서 가까운 열이 바이러스 발생율이 높았고 측창에서 멀어질수록 발생율이 낮아졌다.

재배작형에 따른 토마토황화잎말림바이러스병의 발병양상

고숙주,최덕수,마경철,김도익,김미경,최홍수,김현우 한국식물병리학회 2014 식물병연구 Vol.20 No.4

Tomato yellow leaf curl virus (TYLCV) disease survey of tomato plants cultivated at Youngam, Changseong andHwasun in Jeonnam Province was conducted in 2012 to 2013. To detect TYLCV in the field, PCR assay was performedusing genomic DNAs extracted from tomato samples. According to cropping pattern, 23 and 59.4% ofTYLCV infections were shown in semi-forcing and retarding cropping systems, respectively, and the frequenciesof diseased plants were 0.4 and and 13.1% in the two cropping systems, respectively. Especially, TYLCVincidences in the susceptible varieties were 24.3 and 83.0% in semi-forcing and retarding cropping systems,respectively. Resistant varieties showed 9.1% infection in retarding cropping system but did not in semi-forcing cropping system. Also, TYLCV was occurred on early June after transplanting on April and at approximate 20 days after transplanting on August or September. Tomato yellow leaf curl virus (TYLCV) disease survey of tomato plants cultivated at Youngam, Changseong and Hwasun in Jeonnam Province was conducted in 2012 to 2013. To detect TYLCV in the field, PCR assay was performed using genomic DNAs extracted from tomato samples. According to cropping pattern, 23 and 59.4% of TYLCV infections were shown in semi-forcing and retarding cropping systems, respectively, and the frequenciesof diseased plants were 0.4 and and 13.1% in the two cropping systems, respectively. Especially, TYLCVincidences in the susceptible varieties were 24.3 and 83.0% in semi-forcing and retarding cropping systems,respectively. Resistant varieties showed 9.1% infection in retarding cropping system but did not in semi-forcingcropping system. Also, TYLCV was occurred on early June after transplanting on April and at approximate20 days after transplanting on August or September.

전남지역의 벼 줄무늬잎마름병 발생 분석

고숙주,강범룡,김도익,최덕수,김선곤,이관석,김창석,최홍수,김홍재,김정수 한국식물병리학회 2011 식물병연구 Vol.17 No.3

전남지역에서 벼 줄무늬잎마름병에 대한 연구를 2008년부터 2011년까지 4년간 수행하였다. 전남지역 벼에서 벼 줄무늬잎마름병 발생은 서남해안지역인 진도, 완도, 신안, 해남, 무안에서 2008년부터 2009년까지 피해가 심하였으나, 2010년은 거의 피해가 없었다. 월동 애멸구의 벼 줄무늬잎마름바이러스 보독율은 2008년부터 2011년까지 연도별로 각각 4.7%, 11.3%, 8.7%, 12.8%이었으며, 진도, 완도, 신안 등 서남해안 지역에서 보독충율이 높게 나타났다. 동력흡충기를 이용한 논에서 ㎡ 당 월동 애멸구 밀도는 3월에 높았고, 4월에는 낮아지는 경향이었으며 이는 4월에 경운에 의해 밀도가 낮아지는 것으로 추정되었다. 논에서 애멸구 ㎡당 평균밀도는 3년동안 7.7, 5.4, 4.0마리였다 Occurrence of Rice stripe virus (RSV) was investigated in Jeonnam province from 2008 to 2011. Incidence of RSV was surveyed in paddy fields during growth stage. In western and southern coast parts of 5 districts,Jindo, Wando, Shinan, Haenam and Muan, rice was severely damaged by RSV from 2008 to 2009. But, RSV didn't almost occur in 2010. Viruliferous ratio (VIR) of RSV from overwintered small brown plant hopper (SBPH, Laodelphax striatellus) showed 4.7%, 11.3%, 8.7%, and 2.8% from 2008 to 2011. It was highly recorded in Jindo, Wando, and Shinan of southwestern area. SBPH was collected by sucking machine at 1 m2with 3 replications per paddy field in March. The density of SBPH was high on March, but low on April in non-cultivated barley field from winter to spring. Farmer's plowing this season assumed to be a cause of SBPH density decrease. The number of SBPH was 7.7, 5.4, and 4.0 per m2 during three years, respectively.

ZYMV 감염시기가 오이 생육 및 수량에 미치는 영향

고숙주,김도익,강범룡,김선곤,이용환,최홍수 한국식물병리학회 2009 식물병연구 Vol.15 No.3

We investigated the effect of the infection times and infection degrees at transplanting time of Zucchini yellow mosaic virus (ZYMV) on the growth and yield of cucumber plants at the semi-forcing and the retarding culture in 2007. When cucumber was inoculated with ZYMV at transplanting time, 15, 30, 45, 60, and 75 days after transplanting, vine length, internode length, number of leaf of the plants and marketable yield largely decreased as the cucumber infected earlier. The regression models were obtained between the disease incidence levels at 20 days after transplanting as the inoculation degrees and the marketable yield of cucumber: y = 9333.1e−0.0317x (R2 = 0.8946) at the semi-forcing culture and y = 14695e−0.0303x (R2 = 0.8735) at the retarding culture. And the cucumber yield loss regression models between the symptoms incidence days from the final harvesting time and the rates of yield decrease were expressed as y=1.0851x − 6.7067 (R2 = 0.9567) at the semi-forcing culture andy=1.0439x + 2.1321 (R2 = 0.9674) at the retarding culture.

토마토품종의 토마토황화잎말림바이러스병에 대한 저항성 평가

고숙주,김효정,이진희,마경철,최덕수,박영훈,최승국,김미경,최홍수 한국식물병리학회 2016 식물병연구 Vol.22 No.4

Tomato yellow leaf curl virus (TYLCV) is a viral disease causing severe economic losses on tomato. Practical prevention of the TYLCV disease is to control tabacco whitefly (Bemisia tabaci) or to cultivate TYLCV-resistant tomato cultivars, because no agrochemical products are available to control TYLCV. In this study, TYLCV resistance of the commercial tomato cultivars were evaluated using the DNA markers tightly linked to TYLCV resistance genes Ty-1 and Ty-3 and infection with the TYLCV clones mediated by Agrobacterium. In marker genotyping, resistance alleles were detected from 4 oval type tomato cultivars (Titichal, TY tinny, TY saengsaeng II, TY sense Q). Four cheery type cultiavrs (TY endorphin, TY smartsama, Tiara TY, Olleh TY) and 6 round type cultivars (TY kingdom, TY ace, TY homerun, TY altorang, Dotaerang TY winner, Styx TY). The seedling bioassay indicated that tomato cultivars of the oval type and cherry type showed consistancy in marker genotype and phenotype while slight disease symptom was observed from some round type cultivras (TY ace, TY homerun, Styx TY) with resistance marker genotype. For fruit yields, TY tinny was greater than its control cultivar Titichal in oval types, TY smartsama was greater than its control Smile in cherry type, and TY ace and TY kingdom were greater than their control Dabok. These cutliavrs can be a good choice for high-yielding TYLCVresistant tomato cultivars.