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Broad Bean Wilt Fabaviruses and Their Specific Ultrastructures
최홍수,조점덕,이금희,김정수,Choi, Hong-Soo,Choi, Jeom-Deog,Lee, Keum-Hee,Kim, Jeong-Soo Korean Society of Electron Microscopy 2001 Applied microscopy Vol.31 No.3
잠두위조바이러스(broad bean wilt virus) 5종의 분리주에 대하여 명아주 등 29종의 지표식물반응에 의한 병원성을 분류하였다. 이들 분리주에 감염된 세포에서 3종류의 서로 다른 특이한 미세구조가 관찰되었다. 첫번째 구조는 바이러스입자로 된 $1\sim2$층의 원형관(tube)이고, 둘째는 6각형의 벌집구조(comb)로서 이것은 외부모양이 원형 또는 각으로 된 구조로 구분되었으며, 셋째는 세포원형질 내에 존재하는 다량의 막구조(membrane proliferation)이었다. Pathogenicities of the five BBWV isolates were differentiated by the reactions on the 29 host plants including Chenopodium amaranticolor. Three specific ultrastructures were observed in cells infected with BBWV The first ultrastructure was the tube made of $1\sim2$ layers of virus particles. The second one was the comb structure consists of round and angled structures. The last one was the membrane proliferation in the cytosol.
崔洪守 慶尙大學校 1984 論文集 Vol.23 No.2
Optical absorption, thermally stimulated luminescence(TSL) and TSL emission spectra of uv-grade single-crystal Al₂O₃have been conducted in the temperature range 77-300K. Two kinds of samples was studied. CS sample had been grown in reduced atomosphere environment and Linde sample in oxidizing environment. A typical absorption curve of CS sample exhibits a intense peak near 6.1eV. Linde sample shows a intense peak near 6eV and two weak peaks at 5.4eV and 4.8eV after neutron irradiation. Glow curves of CS sample resulting from uv irradiation at 77k exhibit maxima at 258K (thermal activation energy calculated by Initial Rise method: E=0.72eV, emission spectrum peak:410nm) and 285K(E could not be deduced). A new peak was appeared at 225K(E=0.56eV, 410nm) when bleached at 200K. From Linde crystal, a intense glow peak was observed at 285K(E=0.77eV, 320nm) and weak one at 220K(E=0.53eV, 315nm). The dependence of the intensity of glow peaks on the excitation wavelength was also examined. Experimental results suggest that the 258K and 225K peaks of CS sample are due to the thermal release of trapped electrons which recombine with existing F+ centers producing F centers in an excited state. De-excitation of these centers produces near 410nm radiation. The 285K and 220K peaks of Linde sample are attributed to the thermal release of trapped holes that recombine with electrons of the F centers converting them to excited-state F+ centers which relax to the ground state emitting near 320nm radiation.
Virulence Differentiation of Eight Turnip mosaic virus Isolates Infecting Cruciferous Crops
최홍수,윤무경,최장경,김국형,천정욱,Seong-Han Sohn,Jeong-Soo Kim,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.4
Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Tu 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pf1, r 9-10, Rhcq1- 2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r 9-0,Stock and Rh-cq1-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pf1 isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.
Occurrence of Bean common mosaic virus (BCMV) Infecting Peanut in Korea
최홍수,전정욱,최장경,김미경,박진우,김국형,김정수,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.1
A virus causing vein banding, sometimes yellow mosaic and rugose symptoms on peanut was prevalent around Suwon, Korea. A survey conducted in the area found disease incidence, depending on cultivar, to range from 79 to 100%. The virus was found to be seed-transmissible in all the five peanut cultivars tested with transmission rates ranging from 2 to 16%. Host range analysis failed to differentiate 9 field isolates collected from different peanuts cultivars showing various symptoms. Inclusion bodies such as scroll, pinwheel and long laminated aggregates induced by the virus in host plant cells were similar to those induced by members of the Potyvirus subdivision III. The virus showed < 95% homology with Bean common mosaic virus (BCMV), BCMV-BICMV/AzMV strains and only < 91% with Desmodium mosaic virus. Based on biological characterization, electron microscopy and molecular analyses of a Korean isolate (Daewon 1), the virus was identified as peanut stripe strain of BCMV.
최홍수,박진우,김국형,이수헌,김정수,김미경,최장경,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.1
A virus causing chlorotic ringspot, yellow mosaic and vein clearing symptoms was prevalent on mungbean plants around Taean, Korea. The isolate caused mosaic on Chenopodium quinoa, Nicotiana benthamiana, Phaseolus vulgaris and Vicia faba but no symptoms on peanut plants. Inclusion bodies such as scroll, pinwheel and laminated aggregates induced by the virus in the host cells were similar to those produced by members of the Potyvirus subdivision III. Multiple alignment as well as cluster dendrograms of the 709 nucleotide region comprising part of the coat protein gene and 3'- untranslated region (UTR) showed that the isolate belongs to the BCMV-PSt subgroup. Altogether, these results support the identification of the causal virus as peanut stripe strain of Bean common mosaic virus (BCMV-PSt).
최홍수,Choe, Hong-Su 한국작물보호협회 2012 자연과 농업 Vol.283 No.-
우리나라 파프리카에 발생하는 주요 바이러스는 오이모자이크바이러스(Cucumber mosaic virus, CMV), 고추모틀바이러스(Pepper mottle virus, PepMoV), 잠두위조바이러스2(Broad bean wilt virus2, BBWV2), 고추약한모틀바이러스(Pepper mild mottle virus, PMMoV), 토마토반점위조바이러스(Tomato spotted wilt virus, TSWV) 및 사탕무황화바이러스(Beet western yellows virus, BWYV) 등 6종이다. 생산량 감소 및 고품질 규격품 생산의 가장 큰 제한요인이 되고 있는 바이러스병의 피해를 최소화하기 하기 위하여 바이러스별 병징 및 발생원인에 따른 예방법을 알아본다.