杜仲의 수피와 잎이 난소적출로 유발한 흰쥐의 골다공증에 미치는 영향

오하식,김호철,이상인,안덕균 대한본초학회 1995 大韓本草學會誌 Vol.10 No.1

The purpose of this study is to examination what are the effects of the Eucommiae Cortex fried with salt and Eucommiae Folium on the aged ovariectomized rat model of postmenopaual osteoporosis. The results of the experiment are as follows, 1. The level of serum ALP in Eucommiae Folium group showed a significant increase. 2. The level of S-GOT in Eucommiae Folium group and Eucommiae Cortex group showed a significant decreaseand Eucommiae Folium, but the level of S-GPT in both group showed no efficacy. 3. Urinary volume in Eucommiae Folium group and Eucommiae Cortex fried with salt group showed a significant decrease. 4. The level of urine-calcium to creatinine ratio in Eucommiae Folium group and Eucommiae Cortex fried with salt group showed a significant decrease. According to the above results, effects of the Eucommiae Cortex fried with salt and Eucommiae Folium on the aged ovariectomized rat model showed a efficacy in assistance of hepatic function, prevention of bone aborption and bone mineral-loss, and prevention of urinary volume increase by ovariectomy. And Eucommiae Folium showed a significant to bone-formation, so it can be used for restraint of postmenopaual oseoporosis. As for its effects, continuous clinical study is needed.

當歸類가 혈관신생에 미치는 영향에 대한 비교연구

吳霞植,金亨煥,安德均,崔湖榮 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

A rat sponge implant model was used to examine the angiogenic effect of water extract of Angelica Gigantis Radix (EAG). Angelica Sinensis Radix (EAS) and Angelica Radix (EAA). It was based on subcutaneous implantation of sterile polyester sponges in the Sprague Dawley (SD) rat and we have characterized a dye method that incorporates 5% carmine red in 10% gelatin. Vascularity index (V.I.) expressed as ㎍ dye/㎎ dry tissue. The result of study is as follows; 1. Subcutaneous implantation of sterile polyester sponges in SD rats enhanced the angiogenesis over 14 days, as determined by measurements of relative angiogenic effects by a vascularity index. 2. Daily oral administration (100 ㎎/㎏ for 14 days) of EAS and EAA produced weak effect on sponge-induced angiogenesis but EAG caused intense neovasculalization. 3. PBS (50 μ1) and EAG (100 ㎎/㎏) - induced angiogenesis represents a special form of angiogenesis. 4. The vasoconstrictor peptidem, angiotensin Ⅱ(AⅡ), in daily doses of 100 nmol, caused an intense neovascularization and coadministration of the AⅡ (100 nmol) and EAG (100 ㎎/㎏), AⅡ and EAS (100 ㎎/㎏) and AⅡ and EAA (100 ㎎/㎏) produced angiogenic effect. Especially, AⅡ (100 nmol) and EAG (100 ㎎/㎏) caused very potent angiogenesis. We have shown that oral administration of EAG (100 ㎎/㎏) induced angiogenesis. Thus the sponge model offers a new means for continuous and reproducible studies on the controlling mechanisms of angiogenesis.

선호부위 생산량 제고를 위한 한국형 종돈개량

오하식,O, Ha-Sik 대한양돈협회 2007 養豚 Vol.29 No.9

Trophoblastic Vesicle과 Estradiol-$17\beta$의 첨가가 가토배의 발달에 미치는 영향

오하식,박충생 한국동물번식학회 1986 Reproductive & developmental biology Vol.10 No.1

This experiment was conducted to determine the effects of trophoblastic vesicles (TV) and estradiol-17$\beta$ on the development in vitro of rabbit embryos. Thirty matured female rabbits were treated with PMSG followed by HCG injection and mating. Embryos were recovered with D-PBS (Dulbecco's Phosphate Buffered Saline) after superovulation, and normally developed to two-to four-cell embryos were used in the subsequent in vitro culture. Basal medium was Medium-199 su, pp.emented with 1.5% bovine serum albumin. Embryo on Day 5 after mating (Day 0) was cut into two or three pieces to remove the embryonic disc. Each piece of tissue was cultured for 24 hours at 37$^{\circ}C$ in 0.5 mlMedium-199 in 5% CO2. During culture, peices of trophoblastic tissue changed into spherical vesicles which were used for co-culture. These spheres were called trophoblstic vesicles. Two-to four-cell embryos were cultured for 4 days in Medium-199 in the absence or presence of trophoblastic vesicle, and two-to four-cell embryos cultured with varing concentration (0, 0.1, 1, 10ng/ml) of estradiol-17$\beta$ for 4 dyas. Culture vessels used were watch glass for coculture with trophoblastic vesicles and micortube for estradiol-17$\beta$ infusion. Compared with the Medium-199 alone as basal culture medium, more blastocysts (46.7% vs 15.1%; P<0.01) and morulae (84.4% vs 56.6%; P<0.05) were developed in the co-culture with trophoblastic vesicles. Estradiol-17$\beta$ infused in culture medium was not effective for embryo development to blastocysts (78.3% in control, 50.0% in 0.1ng/ml, 61.5% in 1ng/ml and 64.4% in 10ng/ml) and also to morulae (91.3% in control, 84.2% in 0.1ng/ml, 92.3% in 1ng/ml and 91.1% in 10ng/ml). Compared with the watch glass culture mehotd, more (P<0.01) blastocysts were developed in microtube culture (78.3% vs 56.6%) and more (P<0.01) morulae in microtube culture (91.3% vs 56.6%).

냉동 돼지고기 수입 이렇게 대처하자-종돈부문

오하식,O, Ha-Sik 대한양돈협회 1995 養豚 Vol.17 No.1

사육단계 HACCP 적용 우수농가 사례

오하식,O, Ha-Sik 대한양돈협회 2006 養豚 Vol.28 No.9

돼지 품종의 교배조합간 육질특성 비교

오하식,김현열,양한술,이정일,주영국,김철욱,Oh, Ha-Sik,Kim, Hyun-Youl,Yang, Han-Sul,Lee, Jeong-Ill,Joo, Young-Kuk,Kim, Chul-Uk 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.2

This study was conducted to investigate the meat quality characteristics of pork loin in 3 crossbred groups (30 pigs/group). The crossbreeds were LY female${\times}$D male, Y female${\times}$B male and L female${\times}$B male (L: Landrace, Y: Yorkshire, B: Berkshire, D: Duroc). Pork loins were collected from animals ($110{\times}120kg$ body weight) slaughtered in a commercial slaughter house and stored at $-3^{\circ}C$. Samples were analyzed for general composition, physico-chemical properties, meat and fat color, texture characteristics, sensory evaluation and fatty acid composition at 24 hrs postmortem, and TBARS (Thiobarbituric Acid Reactive Substances) values were determined at various storage times. The LYD pigs showed a higher back fat thickness than the YB and LB pigs. There were no significant differences in intramuscular fat, intermuscular fat, subcutaneous fat and springiness among the 3 crossbreeds. In addition, there were no significant differences in general composition, pH, drip loss, cooking loss and cooked sensory evaluation among the 3 crossbreeds (p>0.05). The shear force value of LB pork was significantly lower than other crossbreeds (p<0.05). Total heme pigment was significantly higher in YB pork compared to LYD and LB pork (p<0.05). The TBARS value of YB pork was significantly lower than that of LYD pork, except after the I day of storage (p>0.05). TBARS values increased significantly during storage for all three crossbreeds. The lightness (CIE L) of YB pork was significantly lower, while the redness (CIE a) was significantly higher in YB pork relative to the other crossbreeds. With regard to textural properties, the hardness and adhesiveness values were significantly lower in YB pork compared to LB pork. In fresh meat, the color of YB pork was significantly higher than the other breeds (p<0.05). The content of myristic acid (C14:0) was significantly lower, while the content of arachidonic acid (C20:4) was significantly higher in LB pork (p<0.05). These results show that the YB breed has the highest quality meat of the three crossbreeds.

'98년도 제2검정소 종돈능력검정 결과

오하식,O, Ha-Sik 대한양돈협회 1999 養豚 Vol.21 No.3

학술발표회 : 육종 ( 구두발표 ) ; 동원체지수 및 상대적길이에 의한 돼지의 품종별 핵형분석`

김철욱,손시환,김형균,오하식 한국동물자원과학회 ( 구 한국축산학회 ) 1994 한국축산학회 심포지움자료집 Vol.- No.-

검정종료돈의 체중변화에 따른 일당중체량, 등지방두께 및 정육율의 반복력과 상관

김현철,김병우,송광림,오하식,손창준,하동우,이정규 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.5

본 연구는 1999년 8월부터 2000년 2월까지 공인 제2종돈능력검정소에서 검정 종료된 Duroc종, Landrace종 및 Yorkshire종에 대하여 체중, 등지방두께, 일당증체량 및 정육율을 측정하여, 동일 개체의 반복측정치 간의 반복력과, 상관 및 순위상관을 추정하였으며, 그 결과를 요약하면 다음과 같다. 1. 평균능력은 측정시 체중이 각각 91.0, 114.7, 123.2㎏이였으며, 측정시 일령은 각각 142.1, 173.7, 182.5일이였으며, 정육율은 각각 57.9, 56.2 55.2%였으며, 등지방두께는 각각 1.33, 1.61, 1.63㎝였으며, 일당증체량은 각각 946.6, 879.2, 879.4g으로 나타났다. 2. 각 형질의 측정차수간 상관은 정육율의 경우, 1차와 2차, 1차와 3차, 2차와 3차간 각각 0.493, 0.471, 0.619로 나타났고, 등지방두께의 경우, 각각 0.424, 0.424, 0.700으로 나타났으며, 일당증체량의 경우, 각각 0.716, 0.601, 0.861로 모든 형질에서 1차와 2차간, 1차와 3차간 보다는 2차와 3차간에 높게 추정되었다. 3. 각 측정차수의 형질간 상관은 1차 측정의 경우, 정육율과 등지방두께, 정육율과 일당증체량, 등지방두께와 일당증체량이 각각 -0.597, -0.291, 0.303으로 나타났고, 2차 측정의 경우, 각각 -0.667, -0.292, 0.396으로 나타났으며, 3차 측정의 경우, 각각 -0.711, -0.369, 0.409로 정육율과 등지방두께 및 일당증체량은 부의 상관관계에 있으며, 등지방두께와 일당증체량은 정의 상관관계에 있고, 일령이 증가할수록 상관이 높게 추정되었다. 4. 각 형질의 반복력 추정치를 살펴보면, 등지방두께가 0.374, 정육율이 0.428, 일당증체량이 0.673으로 추정되었으며, 일당증체량의 반복력은 상당히 높게 추정되어, 110㎏까지 검정의 필요성이 인정되지 않으나, 등지방두께와 정육율의 추정치는 낮게 추정되어 더욱 많은 연구가 필요할 것으로 사료된다. The respeatability, correlation and rank correlation coefficients among average daily gain, backfat thickness and lean percent were estimated on the basis of records tested from August 1999 to February 2000 with 695 pigs of Duroc, Landrace and Yorkshire boars and gilts tested at 2nd Korea Swine Test Station located in Ha-dong, Kyeongnam Province. The effect of the sex, breed and month of measured were estimated by the least square method. The repeatabilities were estimated from the component of variance among repeated measurements of the trait for the same animal. The results obtained are summarized as follow ; 1. The means of the major economic traits studied were 142.1 days, 173.7 days and 182.5 days for age at 1st, 2nd and 3rd measure, 57.9%, 56.2% and 55.2% for lean percent at 1st. 2nd and 3rd mesure, 1.33㎝, 1.61㎝ and 1.63㎝ for backfat thickness at 1st. 2nd and 3rd measure, 946.6g, 879.2g and 879.4g for average daily gain at 1st. 2nd and 3rd measure, respectively. 2. The correlation coefficients between the backfat thickness measured at 1st and 2nd, at 2nd and 3rd, at 1st and 3rd were 0.424, 0.700 and 1.424, respectively. The correlation coefficients between the lean percent measured at 1st and 2nd, at 2nd and 3rd, at 1st and 3rd were 0.493, 0.619 and 0.471, respectively. The correlation coefficients between the average daily gain measured at 1st and 2nd, at 2nd and 3rd, at 1st and 3rd were 0.716, 0.861 and 0.601, respectively. 3. The rank correlation coefficients between backfat thickness measured at 1st and 2nd, at 2nd and 3rd, at 1st and 3rd were 0.438, 0.693 and 0.441, respectively. The rank correlation coefficients between lean percent measured at1st and 2nd, at 2nd and 3rd, at 1st and 3rd were 0.508, 0.593 and 0.478, respectively. The rank correlation coefficients between average daily gain measured at 1st and 2nd, at 2nd and 3rd, at 1st and 3rd were 0.704, 0.834 and 0.571, respectively. 4. The estimated repeatabilities of the traits studied were 0.428 for the lean percent, 0.374 for the backfat thickness and 0.673 for the average daily gain, respectively.