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일본산메추리(Coturnix coturnix japonica)의 염색체 다형현상
손시환 한국가금학회 1990 韓國家禽學會誌 Vol.17 No.4
Constitutive heterochromatin의 염색체 다형현상에 대해 사람을 비롯하여 돼지, 생쥐, 말, 닭 등에서 보고된 바 있다. 본 연구에서는 일본산메추리의 C-band 다형체 뿐만 아니라 염색체의 형태적 다형체를 발견하여 이의 다형현상을 밝혔다. 일반적인 염색체 분석방법 및 C-banding 방법으로서 밝힌 3가지 염색체 다형체는 4번 염색체 +/- 동형체, +/- 이형체 및 -/- 동형체이다. 이와 같은 다형체들은 무작위 집단 내에서 일반적이고 지속적으로 나타나며 Mendel 법칙에 따른 유전양상을 보인다. 따라서 본 연구에서 밝힌 염색체 다형체들은 여러 세포유전학적 연구에 표식인자(chromosome marker)로서 유용하게 이용되어질 수 있을 것으로 생각된다. Comosomal polymorphism involving constitutive heterochromatin has been reported in mu, pigs, mouse, horse, chicken and so on. The chromosomal polymorphism of Japanese quail which includes constitutive heterochromatin as well the chromosomes without banding treatment has now been found. Through the use of a general technique that permits visualization of chromosome morphology and heterochromatin, three chromosomal variants have been found among birds ; +/+ homozygous from, +/- heterozygous form and -/- homozygous form in chromosome 4. This variants appear to be common in the randombred population and stably inherited in a Mendelian fashion. These results suggest that the variants would be useful as chromosomal markers for various types of cytogenetic studies.
가시오갈피와 두충의 첨가 급여가 브로일러의 생산 능력, 혈장 생화학 지표 및 텔로미어 함량에 미치는 영향
손시환,장인석,문양수,김영주,이수희,고영현,강선영,강혜경 한국가금학회 2008 한국가금학회 정기총회 및 학술발표회 Vol.25 No.-
An experiment was conducted to investigate the effect of Siberian ginseng leaf and Eucommia leaf at level of 0.5% and 1% per feed in Ross commercial broiler for 4 to 35 days of age on performance, organ weight, blood biochemical profiles and telomere quantity. Chickens consuming diets containing 1% Siberian ginseng had higher feed conversion ratio than the other treated chicken during experimental period whereas no significant differences were detected in body weight, weight gain and feed intake. The weight of bursa of fabricius was significantly increased in chickens with dietary supplementation compared with chickens fed control but this was not seen in liver, spleen and thymus. In blood biochemical profiles, chickens with dietary supplementation had higher concentration than chickens fed control in triglyceride, cholesterol and glucose. The concentration of aspartate aminotransferase, alanine aminotransferase, albumin and total protein, however, was not significantly different between dietary supplemented chickens and control chickens. The relative amount of telomeric DNA of lymphocytes in chickens with dietary supplementation was significantly higher than that of control chickens but the difference was not found in liver, heart and testis tissues. In conclusion, dietary supplementation of Siberian ginseng and Eucommia in broiler improved immune activity and telomere length without decreasing chicken growth performance.
손시환,김기곤,신가빈,이슬기,이준호,장수용,허정민,추효준 한국가금학회 2023 韓國家禽學會誌 Vol.50 No.3
본 연구는 산란능력이 우수한 토종 산란형 종계를 개발하기위하여 토착 로드종(Rhode-C, -D), 토착 레그혼종(Leghorn-F, K) 및 한국재래계 황갈색종(KNC-Y) 총 5계통을 이용하여 5× 5 이면교배조합(dialel cros-mating) 검정시험을 실시하였다. 생산능력의 검정은 10개 교배조합 1,060수를 대상으로 생존율, 체중, 초산일령, 난중 및 산란율을 조사하고, 조합별 생산능력 및 결합능력을 분석하였다. 분석 결과, 발생 후부터 48주령까지 전체 조합의 평균 생존율은 72.1±24.0%이고, 조합 간 유의적 차이를 보이며 YC조합의 생존율이 가장 높았고, DK조합의 생존율이 가장 낮았다(P<0.01). 결합능력 분석 결과, YC조합의 일반결합능력이 가장 높게 추정되었고, YD조합은 두 계통 간 특정결합능력이 가장 높았다. 체중의 경우, 모든 측정 주령에서 조합 간 유의한 차이가 있었는데 토착 Leghorn종과의 조합 자손들이 토착 Rhode종 및 한국재래계와의 조합 자손들보다 낮은 체중을 보였다 (P<0.01). 초산일령 또한 조합 간 유의한 차이를 보이며 KNC-Y와의 교잡 자손들이 토착 Rhode종과의 교잡 자손들보다 대체로 빠른 초산을 나타내었다(P<0.01). 산란율에 있어, 평균 일계산란율은 70.7±12.0%로써, 조합 간에 유의한 차이를 보이며 이들 중 CK조합이 86%로 가장 높은 산란율을 나타내었다(P<0.01). 산란율의 일반결합능력과 특정결합 능력이 조합가에 미치는 영향은 거의 비슷한 것으로 일반결합능력은 Rhode-C가 가장 우수하였고, 교배조합 간 특정결합능력은 YD조합이 가장 높게 나타났다. 시산 및 40주령 난중 모두 조합 간의 유의한 차이를 보였고(P<0.01), 토착Rhode종과 토착 Leghorn종 간 교잡 자손의 달걀이 KNC-Y 교잡 자손들의 달걀보다 무거웠다. 이상의 결과에 따라 산란형 토종닭 종계로서 부계는 생존율이 우수하고 산란율이 양호한 YC나 YD조합이, 모계는 산란능력이 우수하며 적절한 난중을 지닌 CF와 CK조합이 가장 바람직한 조합으로 사료된다. We conducted a 5 × 5 dialel cros-combination test using 1,060 chickens from pure lines of Korean Rhode-C, -D, Korean Leghorn-F, -K, and Korean Native Yelowish-brown chicken (KNC-Y) to develop a new Korean native chicken layer breder. The laying performance and combining abilty, including livabilty, body weight, age of first eg-laying, hen-day egg production, and eg weight, were analyzed. The livabilty from birth to 48 weks was 72.1±24.0%, with the highest observed in the YC and the lowest in the DK combination (P<0.01). The YC combination exhibited the highest general combining abilty (GCA), while the YD combination showed the highest specific combining abilty (SCA). Regarding body weight, combinations involving Leghorn showed lighter weights compared to combinations with Rhode and KNC-Y (P<0.01). Aditonaly, the ofspring from the KNC-Y combination reached sexual maturity earlier than those from the Rhode combination. The hen-day eg production was 70.7±12.0%, with the highest sen in the CK combination at 86% (P<0.01). The efects of GCA and SCA on hen-day eg production were similar, with the SCA being highest in the YD combination and the GCA being highest in the Rhode-C. Significant diferences in eg weight were observed among the combinations, with the egs from Rhode and Leghorn combinations being heavier than those from combinations with KNC-Y (P<0.01). In conclusion, the YC and YD combinations, characterized by excelent livabilty, are highly desirable paternal strains, while the CF and CK combinations, with excelent laying performance and moderate eg weight, are prefered maternal strains for Korean native chicken layer breeders.
혈액세포를 이용한 염색체 분리 분석에 관한 방법적 고찰
손시환,정구민,Sohn, S.H.,Chung, K.M. 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.2
This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37$^{\circ}C$ for 3 days. And the concentration of colcemid and reincubation time also affected the chromosome morphology. In harvest, chromosome patterns were mainly affected on hypotonic treatment which included treated time and temperature, dropwise of fixative solution, and drying after slide preparation.