Studies on Lytic, Tailed Bacillus cereus-specific Phage for Use in a Ferromagnetoelastic Biosensor as a Novel Recognition Element

( In Young Choi ),( Joo Hyeon Park ),( Kyoung Min Gwak ),( Kwang-pyo Kim ),( Jun-hyun Oh ),( Mi-kyung Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1

This study investigated the feasibility of the lytic, tailed Bacillus cereus-specific phage for use in a ferromagnetoelastic (FME) biosensor as a novel recognition element. The phage was immobilized at various concentrations through either direct adsorption or a combination of 11-mercapto-1-undecanoic acid (11-MUA) and [N-(3-dimethylaminopropyl)-N’-carbodiimide hydrochloride and N-hydroxysuccinimide (EDC/NHS)]. The effects of time and temperature on its lytic properties were investigated through the exposure of B. cereus (4 and 8 logCFU/ml) to the phage (8 logPFU/ml) for various incubation periods at 22°C and at various temperatures for 30 and 60 min. As the phage concentration increased, both immobilization methods also significantly increased the phage density (p < 0.05). SEM images confirmed that the phage density on the FME platform corresponded to the increased phage concentration. As the combination of 11-MUA and EDC/NHS enhanced the phage density and orientation by up to 4.3-fold, it was selected for use. When various incubation was conducted, no significant differences were observed in the survival rate of B. cereus within 30 min, which was in contrast to the significant decreases observed at 45 and 60 min (p < 0.05). In addition, temperature exerted no significant effects on the survival rate across the entire temperature range. This study demonstrated the feasibility of the lytic, tailed B. cereus-specific phage as a novel recognition element for use in an FME biosensor. Thus, the phage could be placed on the surface of foods for at least 30 min without any significant loss of B. cereus, as a result of the inherent lytic activity of the B. cereus-specific phage as a novel recognition element.

Hydrotalcite의 製造條件에 關한 硏究

안세민,신화우,최광식,이광표,장영수 원광대학교 식품약품안전성연구소 1993 食品藥品安全性硏究 Vol.6 No.-

Hydrotalcite is mainly used as a antacid and adsorbent. It is Well Known that the yield of hydrotalcite produced is greatly affected by the preparation conbition such as the reactant concetration, reaction temperature, reaction time, mole ratio of reactants and grying tepeeature, etc. the purpose of this study is to investigate the optimum preparation condition of hydrotalcite. Arandomived complete block design suggested by G.E.P.Box and K.B. Wilson was appliedfor this purpose. Hydrotalcite was prepared by reacting sodium carbonate. Magnesium oxide and Aluminum sulfate solutions in this study. The optimum preparation condition of hydrotalcite obtained from this study is as follows: 1) The reaction temperature range is 48-63℃ 2) The concentration of reactant solutions is about 20% 3) The optimum mole-ratio (MgO/Al. sulfate) is 7.35-8.1. 4) The reacting time tatge is 10-11 min.. 5) The drying temperature range is 78-82℃ The outcome of D.S.C. indicated a desolvation of hydrotalcite occured at about 98℃ The dehydration of the compound ceased at about 250℃, and the decarboxylation ceased at about 446℃ The physical and chemical properties of hydrotalcite as medicine were studied by use of chemical analysis, bulk volume test and acid consuming capacity measurements.

Thermomicrobium roseum 유래 Alcohol Dehydrogenase의 정제 및 특성

윤석영,홍민표,김은호,공광훈 중앙대학교 기초과학연구소 2000 基礎科學硏究所 論文集 Vol.14 No.-

Alcohol dehydrogenase from Thermomicrobium roseum was purified to electrophoretic homogeneity approximately 77-fold with a 38% activity yield by adenosine 5'-monophosphate and Mono Q column chromatography. The molecular weight of the enzyme was determined to be approximately 43,000 by SDS-polyacrylamide gel electrophoresis and 88,000 by gel chromatography, indicating a homodimeric structure. The Km and Vmax values of the enzyme for ethanol were 24.2 mM and 12.8 ㎛ol/min/mg, respectively. Pyrazole notably inhibited the enzymatic activity. Ca2+ ion activated the enzyme activity, but Cu2+ inhibited. The activity of the enzyme was optimal at pH 10.0 and 60℃.

습기제거시스템의 성능에 관한 연구

유성연,정민호,홍정표,심국진,김광영 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

The performance of moisture removal system is investigated analytically and experimentally. The program for the moist air calculation and for the condensing tube design are developed to examine the factors which affect moisture removal rate. Experimental apparatus comprises condensing tube, cooling system, vacuum pump, measurement and control devices. Thermoelectric cooling system and vapor compression refrigeration system are used to cool the air, and the performance of two systems are compared each other. Thermoelectric cooling system is compact and free from noise and vibration, but cooling rate of it is relatively slow compared to that of vapor compression refrigeration system. Air velocity, indoor temperature and humidity, temperature of coolant, size and material of condensing tube are found to affect the performance of moisture removal.

분말야금법을 이용한 TiNi기 형상기억 복합재료의 기계적특성에 관한 연구

박영철,윤두표,박민식,김광영 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.1

In this study, It is introduced that TiNi/Al-radical shape memory composite as one of new material that used shape memory alloy (SMA). It is a method to produce a better strength of composite by occuring compressive residual stress in matrix, using shape memory effect of TiNi fiber. This method os replaced of tensile residual stress happened by difference of coeffients of thermal expansion (CTE) between fiber and matrix, one of the significant faults of metal matrix composite (MMC). In this paper, shape memory composites are made by powder metallurgy. And then, an self-strengthening effect of the composites by shape memory effect above inverse transformation temperature A_f of TiNi alloy are discussed. Moreover, TiNiCo/Al composites is made by using TiNiCo alloy as fiber. And it is discussed about affection of Co in the shape memory composite. The results of the intelligent properties of TiNi/Al-radical shape memory composite, using SMA, by powder metallurgy are the tensile strength of TiNiCo wire is much higher than that of TiNi wire and the strength of TiNiCo/Al composite is generally higher than of TiNi/Al composite.

珪酸마그네슘의 合成條件에 關한 硏究

전우정,신화우,최광식,안세민,이광표,장영수 원광대학교 식품약품안전성연구소 1993 食品藥品安全性硏究 Vol.6 No.-

Magesium twisilicate is mainly uaed as a nonsytemic antacid andabsrbent. Magnesium trisilicate when teeated with anacid sxuh as diluted hydrochloric acid in gastric juice decomposes to form Magnesium chloride and colloidal Silicates. 2MgO·3SiO_2 + 4Hcl - 2MgCl_2 + H_4Si_3O_8 (or 3SiO_2 + 2H_2O) Colloidal mixture The resultng colloidal silicates can protect ahe ulcer frdm further acid and peptic attack and possibly adsorb the pepsin. It is well known that yield of Magnesium trisilcate produced is greatly affected by the Synthesis condition such as the reactant concentration, reaction temperature and moleatio of reactant solutions as well as by the emperature at which the precipitate is dried and the temperature of washing water, etc. The purpose of this study is to investigate the optimum synthesis conditions of Magnesium trisilicate. A randomized complete block design suggested by G.E.P. Bdx and K.B. Wagnesium sulfate solution with Sodium silicate solution in this study. It was found that optimal synthesis conditidns were: Reaction temperature range : 57-90 ℃. Concentration range of reactants (Sodium silicate and Magnesium sulfate) : 19.1-29% molar ratio of the reactants. [Sodium silicate]/[Magnesium sulfate]: 1.47-1.80. temperature range of washing water: 45-48℃ and drying temperature range: 65-82℃ The antacidic activity of five Magnesium trisilicate samples which shows the maximum antacidic efficacy was tested by pharmacopeia acid consuming capacity test. The five Magnesium trisilicate samples were samples were identified by chemical analysis.

Box-Wilson 계획법에 의한 沈降炭酸칼슘의 製造條件에 關한 硏究

김영윤,신화우,최광식,안세민,이광표,장영수 원광대학교 식품약품안전성연구소 1993 食品藥品安全性硏究 Vol.6 No.-

Medicinal Precipitated Clacium Carbonate is prepared by the interaction of Calcium Chloride and Ammonium Carbonate Solutions in this study. It has been known that the particle size of Precipitated Calcium Carbonate is greatly affected by the concentration, temperature, and moli-ratio of reactant solutions as well as the temperature at which the precipitate is dried and the temperature of washing water. The purpose of this study is to determine the optimum preparation Condition of medicinal Precipitated Calcium Carbonate. A randomized complete block design wuggested by G.E.P. Box and K.B.Wilson was for this purpose. The optimum preparation condition of light and fine precipitated Calcium Carbonate obtained from this study is as follows : 1) The reacting temperature range is 25∼45℃ 2) The concentration range of reactant solutions is 10∼30% 3) The optimum mole-ratio (CaCl/(NH) CO) is 1.0∼2.0. 4) The drying temperature range is 60∼80℃. 5) Temperature range of washing water is 25∼60℃.

PET/CT 검사에서 호흡에 따른 인공산물을 줄이기 위한 Average CT의 유용성

김정선,남기표,박승용,류재광,차민경,Kim, Jung-Sun,Nam, Ki-Pyo,Park, Seung-Yong,Ryu, Jae-Kwang,Cha, Min-Kyeong 대한핵의학기술학회 2010 핵의학 기술 Vol.14 No.1

종양학에서 암의 진단, 병기 결정, 재발 판정, 그리고 치료에 대한 반응의 평가에 PET/CT의 유용함은 이미 인정되고 있다. 그런데 크기가 작거나 폐의 기저 부위, 혹은 간의 상부에 위치한 종양일수록 호흡으로 인한 위치 변위와 왜곡의 정도가 크며 PET영상의 SUV에도 영향을 미치게 된다. 따라서 정상 호흡 상태에서 얻어지는 PET 또는 CT영상을 토대로 방사선 치료를 하게 되면 치료해야 할 표적이 치료 범위에서 벗어나거나 정상 조직에 과도한 방사선이 조사될 수 있으므로 치료율이 낮아지거나 방사선에 의한 부작용이 증가할 수 있다. 본 논문의 목적은 호흡에 의한 인공산물을 최소화하고 보다 정확한 SUV 측정을 위해 ACT를 이용한 감쇠 보정 방법을 적용하여 그 유용성을 평가하고자 함이다. 하 흉부에 종양이 있는 13명의 환자를 대상으로 Discovery STE8 PET/CT스캐너를 사용하여 두 가지의 PET/CT영상을 얻었다. HCT를 사용한 감쇠 보정 영상과 ACT를 사용한 감쇠 보정 영상에서 측정한 인공물의 크기와 $SUV_{max}$를 비교 분석하였다. 인공물은 모든 환자의 하 흉부의 백색 음영 영역을 측정하여 평가하였다. $SUV_{max}$는 주요 종양의 $SUV_{max}$를 측정하여 평가하였다. 분석 프로그램은 Advantage Workstation v4.3을 사용하였다. 환자에게 7.4 MBq (0.2 mCi)/kg의 $^{18}F$-FDG를 투여한 1시간 뒤 스캔하였다. 방출 스캔은 3 min/bed로 스캔하였다. HCT 보정 영상과 비교하여 ACT 보정 영상에서 인공산물의 크기가 눈에 띄게 줄어든 것을 관찰할 수 있었다. 하 흉부의 저 보정으로 인한 인공산물 크기는 ACT 보정 영상과 HCT 보정 영상 각각 $1.5{\pm}3.5$ cm과 $13.4{\pm}4.2$ cm를 나타냈다. 주요 병소의 $SUV_{max}$의 변화는 ACT 보정 영상이 HCT 보정 영상보다 눈에 띄게 상승하였다. ACT 보정 영상에서 HCT영상과 비교하여 tumor의 $SUV_{max}$가 평균 $5.3{\pm}3.9%$ 상승하였다. 가장 큰 차이를 보인 종양은 lung의 lower lobe에 있는 종양으로 $SUV_{max}$ 7.7에서 $SUV_{max}$ 8.7로 13% 상승하였다. ACT를 이용한 감쇠 보정 영상은 하 흉부의 인공산물을 눈에 띄게 줄일 수 있어 보다 정확한 병소의 SUV를 측정 및 방사선 치료 범위 설정에 도움이 될 수 있을 것이다. 또한 ACT 기법은 병소가 횡격막 부근에 있는 환자의 경우 폐와 간의 경계를 보다 정확히 구분할 수 있어 판독시 도움이 될 수 있을 것이라고 판단된다. 추가 ACT 촬영에 의한 피폭 선량이 증가하는 점을 고려하여 적용한다면 임상적으로 유용한 효과가 있다고 사료된다. Purpose: The usefulness of Positron Emission Tomography (PET) images in diagnosis, staging, recurrent and treatment response evaluation has already been known. However, tumors which are small size, located in lower lobe of lung or upper lobe of liver are shown misalignment, distortion and different Standard Uptake Value (SUV) by respiration in PET images. Therefore, if radiotherapy based on normal respiration, it may cause low treatment response or more side effects because targets which had to treat, out of treat range or over dose to normal tissue. The purpose of this study is to evaluate attenuation-correction with Average CT (ACT) for more accuracy SUV measurement and minimize artifact by respiration. Materials and Methods: 13 patients, who had tumors which are around the diaphragm, underwent ACT scan after Helical CT (HCT) scan with PET/CT (Discovery DSTE 8; GE Healthcare). We quantified the differences between attenuation corrected image with HCT and attenuation corrected image with ACT in artifact size and maximum SUV ($SUV_{max}$). Artifacts were evaluated by measurement of the curved photogenic area in the lower thorax of the PET images for all patients. $SUV_{max}$ was measured separately at the primary tumors. Analysis program was Advantage Workstation v4.3 (GE Healthcare). Patients were injected with 7.4 MBq (0.2 $mC_i$) per kg of $^{18}F$-FDG and scanned 1 hour after injection. The PET acquisition was 3 minute per bed. Results: Significantly lower artifact were observed in PET/ACT images than in PET/HCT images (below-thoracic artifacts caused by under corrected $1.5{\pm}3.5$ cm vs. $13.4{\pm}4.2$ cm). Significantly higher $SUV_{max}$ were noted in PET/ACT images than in PET/HCT images in the primary tumor. Compared with PET/HCT images, $SUV_{max}$ in PET/ACT images were higher by $5.3{\pm}3.9%$ (mean value) tumor. The highest difference was observed in Lower lobe of lung (7.7 to 8.7; 13%). Conclusion: Due to its significantly reduced artifacts in lower thoracic, attenuation corrected image with ACT images provided more reliable $SUV_{max}$ and may be helpful in monitoring treatment response. Moreover, ACT can separate upper lobe of liver and lower lobe of lung, it may be helpful in interpretation. ACT will be clinically useful, considering increased dose caused by ACT scan and adapt.

Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells

Min Jae Kim,Bong-Kwang Jung,Jaeeun Cho,Hyemi Song,Kyung-Ho Pyo,Ji Min Lee,Min-Kyung Kim,Jong-Yil Chai 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.2

주권의 정치와 대화의 정치

표광민(Kwang-Min Pyo) 한국현상학회 2018 철학과 현상학 연구 Vol.78 No.-

이 글은 슈미트와 아렌트의 정치사상을 각각 주권중심 정치와 대화중심 정치로 정의내리며, 양자 사이의 비교를 통해 대화가 정치의 본질임을 주장한다. 냉전 종식 이후 신자유주의 경제 질서는 보편가치의 지위를 획득하며 전 세계적으로 확산되었으나, 테러와의 전쟁, 금융위기 사태 등으로 인해 곧 그 한계에 다다르게 되었다. 신자유주의 세계질서를 대체하기 위한 대안 가운데 칼슈미트와 한나 아렌트의 사상이 이론적으로 관심을 모으고 있다. 두 학자는 모두 이데올로기가 추구하는 세계 보편성에 대해 의문을 제기하며, 정치의 본질을 제한된 영역에서 나타나는 공간성으로 설명한다. 그러나 두 사람은 이 정치적 공간을 구성하는 원리를 각각 주권과 대화로 다르게 제시하고 있으며, 이로 인해 정치와 인간 공동체의 본질 역시 서로 다르게 바라보게 된다. 이 글은 슈미트와 아렌트가 말하는 정치의 공간성 및 이로부터 각자 전개되는 주권정치와 대화정치의 내용을 살펴보려 한다. 단순 비교를 지양한 비판적 독해를 통해, 이글은 적과 동지의 구분에 기초한 슈미트의 주권정치는 국내정치의 소멸로 이어질 수밖에 없다는 점과 아렌트의 대화정치는 주권정치에 대한 직접적 대안으로 제시되었다는 점을 밝히려 한다. 그리고 이로부터, 정치의 본질은 결국은 폭력이 아닌 대화일 수밖에 없음을 주장하려 한다. This paper defines Carl Schmitt’s political thoughts as sovereignty-politics and Hannah Arendt’s as dialogue-politics, and argues that the dialogue is the essence of politics through a comparison between two authors. After the end of the Cold War, the neoliberal economic order gained universal value and spread worldwide, but it soon reached its limit with the war on terrorism and the financial crisis. Among the alternatives to the neoliberal World order, the ideas of Schmitt and Arendt are of theoretical interest. Both authors question the universality of the World pursued by abstract ideas, and explain the spatial dimension of politics which appears in a limited sphere. However, the two present the different principles of the political sphere : sovereignty and dialogue. This article seeks to examine the spatiality of Schmitt’s and Arendt’s politics, and their sovereignty-politics and dialogue-politics. Through the critical reading beyond a simple comparison, this article argues that Schmitt’s sovereignty-politics, based on the distinction between friend and foe, will lead to the disappearance of domestic politics, and that Arendt’s dialogue-politics is presented as a direct alternative to sovereigntypolitics. And from this, it would be elucidated that the essence of politics is the conversation, not violence.